Chd5 (NM_029216) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930532L22Rik; AW060752; B230399N07Rik; CHD-5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225040 representing NM_029216 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCCCGCTGGGCACCGAGGAGGAGCTGCCGCGGCTGTTCGCGGAGGAGATGGAGAACGAGGAGG AGATGTCAGAAGAAGAAGACGGTGGCCTTGAAGGCTTTGAGGACTTCTTCCCTGCAGAGCCCGTGAGTCT TCCCAAGAAGAAGCCGAAGAAGCTGAAAGAGAGCAAGTCCTCCAAAGGTAAAAGGAAGAAGAAAGAGGGG AGCAATGACGAGATGTCAGACAACGAGGAGGACCTGGAAGAGAAGTCAGAGAGCGAAGGTAGCGACTACT CCCCCACCAAGAAGAAGAAGAAGAAACTGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGCGGAA AAAGAGGGGTGAGGATGAAGATGATAACGACGACGGAGGCTTGAAGGAGCCCAAGTCCTCGGGACAACTC ATGGCGGAGTGGGGGCTGGACGACGTGGACTACCTGTTTTCTGAGGATGACTACCACACACTGACCAACT ACAAAGCCTTCAGCCAGTTCCTCAGGCCCCTTATTGCCAAGAAGAATCCGAAGATCCCCATGTCCAAAAT GATGACTGTCCTGGGGGCCAAGTGGCGAGAATTCAGCGCCAACAACCCGTTTAAGGGTAGCTCGGCAGCA GCAGCTGCGGCGGCCGTGGCCGCGGCCGTGGAGACAGTGACCATTGCTCCTCCCTTGGCCATCAGTCCCC AGCAAGTGCCCCAGACTCTGCCCATACGAAAGGCCAAGACCAAAGAGGGCAAGGGGCCCGGAGTGAGGAA GAAGAACAAAGGCGCCAAAGATTCCAAGAAAAAGGGCAGGGGCAAGAGAGTGGCAGGGCTCAAGTTCCGC TTCGGAGGGATCAGCAAGAGGAAGAAGGGTTCCTCGAGCGAGGAGGATGAACGGGAGGACTCAGACTTGG ACAATGCCAGCATCCACAGCTCTTCTGTGCGTTCTGAGTGCTCTGCAGCTCTGGGCAAGAAGAACAAGAG GAGGCGCAAGAAGAAGAGGATTGACGATGGTGACGGCTATGAGACAGACCACCAGGACTACTGTGAGGTG TGCCAGCAGGGAGGCGAGATCATTCTCTGTGACACCTGCCCGAGGGCATATCACCTAGTCTGCCTGGACC CGGAGCTGGAGAAGGCTCCAGAGGGCAAGTGGAGCTGCCCACACTGTGAGAAGGAGGGGATCCAGTGGGA GCCGAAGGATGATGATGAAGAGGAGGAGGAGGGCGGCTGCGAGGAAGAGGAGGATGACCACATGGAGTTC TGCCGCGTGTGCAAGGACGGTGGCGAGCTCCTGTGCTGTGATGCCTGTCCCTCCTCCTACCACTTGCACT GCCTCAACCCGCCGCTGCCGGAGATACCGAACGGTGAATGGCTCTGCCCGCGCTGTACATGTCCCCCGCT GAAGGGCAAAGTTCAGCGGATCCTACACTGGAGGTGGACAGAACCCCCGGCTCCCTTTGTGGTGGGGCTG CCAGGACCAGAGGTGGAGCCAGGCATGCCCCCGCCCAGGCCTCTGGAGGGCATTCCTGAGAGAGAGTTCT TTGTCAAGTGGGCTGGTCTCTCCTACTGGCACTGCTCCTGGGTGAAGGAGCTACAGTTGGAGCTGTACCA CACCGTGATGTATCGCAACTACCAAAGAAAGAATGACATGGACGAACCGCCACCCTTTGATTACGGCTCT GGCGATGAGGATGGTAAGAGCGAGAAACGGAAGAACAAGGACCCTCTCTACGCCAAGATGGAGGAACGCT TCTACCGCTACGGCATCAAGCCCGAGTGGATGATGGTTCACCGCATCCTGAACCACAGCTTTGACAAGAA GGGGGACATACATTACCTGATCAAGTGGAAGGACCTGCCCTACGACCAGTGCACCTGGGAGATTGATGAG ATTGACATTCCCTACTACGACAACCTGAAGCAGGCCTACTGGGGCCACAGGGAGCTGATGCTGGGGGAAG ATGCCAGGCTGCCCAAGCGGCTGGTGAAGAAGGGCAAGAAGCTGAAGGACGATAAACAAGAGAAGCCACC CGATACGCCCATCGTGGACCCCACAGTCAAGTTCGACAAGCAGCCGTGGTACATCGACGCCACAGGAGGC ACGCTGCACCCTTACCAGCTGGAGGGCCTTAACTGGTTACGCTTCTCCTGGGCCCAGGGCACCGACACTA TCCTGGCTGACGAGATGGGTTTGGGGAAGACAGTACAGACCATTGTGTTTCTCTATTCTCTGTACAAGGA GGGCCACTCCAAGGGGCCTTACCTGGTCAGCGCGCCCCTGTCCACCATCATCAACTGGGAACGGGAGTTT GAGATGTGGGCGCCTGACTTTTACGTGGTCACCTACACGGGGGACAAGGAGAGCCGCTCTGTGATCCGTG AGAACGAGTTTTCCTTTGAGGACAACGCCATTCGCGGTGGCAAGAAAGTATTCCGCATGAAGAAGGAAGT GCAGATCAAATTCCACGTGCTGCTCACCTCTTATGAGCTCATCACCATTGACCAAGCCATCCTGGGCTCC ATCGAGTGGGCCTGCCTCGTGGTGGACGAGGCCCACCGGCTCAAGAACAACCAGTCCAAGTTCTTTAGGG TCTTGAATAGCTACAAGATCGACTACAAGCTGCTGCTGACAGGGACCCCCCTCCAGAACAACCTGGAGGA ACTGTTCCATCTCCTCAACTTCCTGACTCCAGAGAGGTTCAACAATCTGGAAGGCTTCTTGGAGGAGTTT GCTGACATATCCAAGGAAGATCAGATCAAAAAGCTGCATGATCTATTGGGGCCGCACATGCTTCGGCGGC TCAAGGCCGACGTGTTCAAGAACATGCCGGCTAAGACAGAGCTGATTGTCCGAGTGGAGCTGAGCCAGAT GCAGAAGAAGTACTACAAGTTCATCCTGACTCGGAACTTTGAGGCGCTCAATTCCAAGGGGGGTGGCAAC CAGGTGTCTCTACTCAATATCATGATGGATCTGAAGAAGTGCTGCAACCATCCGTACCTCTTCCCTGTGG CTGCTGTGGAAGCCCCTGTACTGCCCAATGGCTCCTACGATGGCAGCTCCCTGGTCAAGTCTTCGGGGAA GCTCATGCTATTGCAGAAGATGCTCAAGAAACTGCGGGATGAAGGGCACAGAGTGCTGATCTTCTCCCAG ATGACCAAGATGTTGGACCTCTTGGAGGACTTCCTGGAGTACGAGGGCTACAAGTATGAGCGGATCGACG GTGGCATCACTGGGGGCCTCCGGCAAGAGGCCATCGACAGATTCAACGCTCCTGGGGCCCAACAGTTCTG CTTCCTGCTCTCAACACGTGCCGGTGGCCTGGGCATCAACCTGGCCACAGCAGACACGGTCATCATCTAT GACTCGGACTGGAACCCTCACAATGACATCCAGGCCTTCAGCCGTGCTCACCGCATCGGGCAGAACAAGA AGGTCATGATCTATCGCTTCGTGACACGGGCCTCCGTGGAGGAGCGCATCACTCAGGTGGCCAAGCGTAA GATGATGCTCACACACCTGGTGGTGCGGCCTGGCTTGGGCTCCAAGTCGGGCTCCATGACCAAGCAGGAA CTGGATGATATCCTTAAGTTCGGGACAGAGGAGCTTTTCAAGGACGACGTGGAAGGCGATAACAAAGATG TAGAAGACAGCAGTGTGATCCATTATGACGACGCAGCCATATCCAAGCTGCTGGATCGGAACCAGGACGC CACGGATGACACCGAGCTGCAGAACATGAACGAGTACCTGAGCTCCTTCAAGGTGGCACAGTATGTGGTC CGCGAGGAAGATGGCGTGGAGGAGGTAGAGAGGGAGGTGATCAAGCAGGAGGAGAACGTGGACCCTGACT ACTGGGAGAAGCTGCTGCGCCATCACTATGAACAGCAGCAGGAGGACTTGGCCCGCAACCTGGGCAAGGG CAAGCGCATCCGCAAGCAGGTCAACTACAATGATGCCTCCCAGGAGGACCAGGAGTGGCAGGATGAGCTC TCGGACAACCAGTCGGAGTATTCCATCGGTTCTGAGGATGAGGACGAGGACTTTGAGGAGCGACCAGAAG GGCAGAGTGGACGACGACAATCCAGGAGGCAGCTTAAGAGTGACAGGGACAAGCCCCTGCCTCCCCTTCT GGCTCGGGTTGGGGGCAACATTGAGGTTCTGGGCTTCAATGCCAGACAGAGGAAGGCTTTTTTGAACGCC ATCATGCGCTGGGGCATGCCGCCTCAGGATGCCTTCAACTCCCACTGGCTGGTGCGGGACCTTCGAGGGA AGAGTGAGAAGGAATTTAGGGCCTATGTATCACTCTTCATGCGCCACCTGTGTGAGCCGGGCGCAGACGG CGCGGAGACCTTCGCAGACGGCGTGCCCCGGGAGGGCCTGTCTAGGCAGCACGTGCTCACGCGTATTGGG GTCATGTCGCTGGTTAGGAAGAAGGTGCAGGAGTTTGAGCATGTCAACGGCAAGTACAGCACCCCAGACC TGGTCCCCGAGGGGGCCGAGGGCAAGAAGCCGGGCGAGGTCATCTCCTCAGACCCCAACACACCTGTGCC TGCCAGCCCTGCACAGCTTCCACCGGCCCCACTGGGCCTGACAGACAAAATGGAAGCCCAGCTGGGCTAC ACAGATGAGAAGGAGTCAGGCATGCAGAAGCCAAAGAAGTCCCTGGAAATCCAGACCCTGCCAACTGCCC TGGACAGAGTAGAGGGTGAAGACAAACATCAGAGCTCAGACAGCAAGGACAGGGCTCGGGAGGAGAGGAC AGAAGAGGTGGAGAAGGCCCAGGGGTCTCCAGAGCAGCCACTGAAAGAGGAAGTGCTGCCGGACAAGGAG CCGATCCCAGACAAGCCGGAGCTGAGCTTGGGTCACAGCGGGGATTTCAGGCCAGATGACCCCAAGACTG AGGAGAAGGAGCCCGGGGAGACTCAGCAAAACGGCGACAGAGAGGAAGATGAGGAAGGCAAGAAAGAAGA CAAAAATGGGAAATTCAAATTCATGTTCAACATTGCAGATGGCGGTTTCACAGAGCTGCACACTCTGTGG CAGAACGAGGAGCGGGCGGCTGTGTCCTCGGGGAAAATCTACGAAATCTGGCACCGCCGTCACGACTACT GGCTGCTGGCAGGCATTGTGACGCATGGCTATGCCCGCTGGCAGGACATTCAGAATGACCCGAGATACAT GATCCTAAATGAACCTTTCAAGTCTGAGATCCACAAGGGCAACTACTTGGAGATGAAGAACAAGTTTTTG GCCCGTAGGTTCAAGCTGCTGGAGCAGGCGCTGGTGATTGAGGAGCAGCTCCGGAGGGCAGCGTACCTCA ACATGACCCAGGATCCCAACCACCCAGCCATGGCGCTCAATGCTCGCCTGGCAGAGGTGGAGTGCCTTGC CGAGAGCCACCAGCACCTATCCAAGGAGTCCCTCGCCGGAAACAAGCCTGCCAATGCTGTCCTGCACAAG GTCCTAAACCAGCTGGAGGAGCTGCTGAGTGACATGAAAGCAGATGTGACACGCTTGCCCTCCATGTTGT CACGCATCCCCCCGGTGGCCGCCCGTCTGCAGATGTCTGAACGCAGCATCTTGAGTCGCCTGACCAACCG AGCTGGTGACCCCACCATCCAGCAGGGCGCTTTCGGCTCCTCTCAGATGTACAACAACAGCTTTGGGCCC AACTTCCGGGGCCCTGGACCGGGAGGGATTGTCAACTACAACCAGATGCCCCTGGGGCCCTATGTGACTG ACATCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_029216
Insert Size	5748 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029216.2, NP_083492.2
RefSeq Size	9375 bp
RefSeq ORF	5748 bp
Locus ID	269610
Gene Summary	Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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