Polr2a (NM_009089) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	220kDa; Rpb1; Rpo2-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225050 representing NM_009089 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACGGGGGTGGCCCCCCCTCCGGGGACAGCGCATGCCCGCTGCGCACCATCAAGAGAGTGCAGTTCG GAGTCCTGAGTCCGGATGAATTGAAGCGGATGTCTGTGACAGAGGGTGGTATCAAATACCCAGAAACAAC GGAGGGAGGTCGCCCCAAACTTGGGGGACTAATGGATCCACGGCAGGGGGTGATTGAGCGGACTGGCCGC TGCCAAACATGTGCAGGAAACATGACCGAGTGTCCTGGCCACTTTGGCCACATCGAATTGGCCAAACCTG TGTTTCATGTGGGCTTCCTGGTGAAGACAATGAAGGTTTTGCGCTGTGTCTGCTTCTTCTGCTCCAAACT GCTTGTAGATTCTAATAACCCGAAGATTAAGGACATCCTGGCCAAATCTAAGGGGCAGCCAAAGAAACGG CTTACGCATGTCTATGACCTGTGCAAGGGCAAAAACATCTGTGAAGGTGGAGAGGAGATGGACAACAAGT TTGGTGTGGAGCAGCCGGAGGGTGATGAGGATCTGACCAAAGAGAAGGGCCATGGCGGCTGTGGGCGGTA CCAACCCCGAATCCGGCGCTCTGGCCTAGAGTTGTATGCTGAATGGAAGCATGTTAATGAGGACTCTCAG GAGAAAAAGATCTTGCTGAGTCCAGAACGAGTGCATGAAATCTTCAAACGCATCTCAGATGAGGAGTGTT TTGTGTTGGGCATGGAGCCTCGCTATGCCCGGCCTGAGTGGATGATTGTCACAGTGTTGCCTGTGCCTCC TCTCTCAGTGAGACCTGCTGTGGTGATGCAGGGTTCTGCTCGAAACCAGGATGATCTGACTCACAAACTG GCTGACATTGTGAAGATCAACAATCAGCTGCGGCGGAACGAGCAGAATGGTGCAGCTGCCCATGTCATAG CAGAAGATGTAAAACTTCTCCAGTTCCATGTGGCCACCATGGTGGACAATGAACTGCCCGGCTTACCCCG TGCCATGCAGAAGTCTGGCCGTCCCCTCAAGTCCCTGAAGCAACGGTTGAAGGGCAAAGAAGGACGGGTT CGAGGGAATCTAATGGGCAAGCGAGTGGACTTCTCAGCCCGCACTGTCATCACCCCTGACCCTAACCTAT CCATTGACCAAGTGGGTGTGCCCCGCTCCATTGCTGCCAACATGACCTTTGCAGAGATTGTCACACCCTT CAATATTGACAGACTTCAGGAATTAGTACGCAGGGGAAACAGCCAGTATCCAGGAGCCAAGTACATCATC CGCGATAATGGCGATCGAATTGACTTGCGTTTCCATCCTAAGCCCAGTGACCTTCATCTGCAGACTGGCT ATAAGGTGGAACGGCACATGTGTGATGGGGACATTGTTATCTTCAATCGTCAGCCAACTTTGCACAAAAT GTCCATGATGGGGCATCGGGTCCGAATCCTCCCCTGGTCTACTTTTCGGTTGAATCTTAGTGTGACAACT CCATACAATGCAGACTTTGATGGAGATGAGATGAATTTGCATCTGCCACAGTCTCTGGAAACACGGGCAG AGATCCAAGAGTTGGCTATGGTGCCTCGAATGATTGTCACCCCCCAGAGCAATCGACCTGTCATGGGTAT TGTGCAAGACACACTCACGGCAGTACGCAAATTCACTAAGAGAGATGTCTTCCTGGAGCGGGGTGAAGTC ATGAACCTCTTAATGTTTCTGTCCACGTGGGATGGCAAGGTCCCACAACCAGCTATCCTCAAGCCCCGGC CCCTGTGGACAGGCAAGCAGATCTTCTCCCTCATCATACCTGGACACATCAATTGTATCCGTACCCACAG CACCCATCCTGATGATGAAGATAGTGGCCCTTACAAGCACATCTCTCCTGGGGACACCAAAGTTGTAGTG GAGAATGGGGAGCTTATCATGGGCATCCTTTGTAAGAAGTCCCTGGGCACCTCAGCAGGCTCGCTGGTCC ATATCTCCTACCTGGAGATGGGGCATGACATCACCCGCCTCTTCTACTCCAACATTCAGACTGTCATTAA CAACTGGCTTCTCATCGAGGGTCATACCATTGGCATTGGGGACTCCATTGCAGATTCAAAGACTTACCAG GACATTCAGAACACTATTAAGAAAGCTAAACAAGATGTAATAGAGGTCATTGAGAAGGCTCATAACAATG AGCTAGAACCCACTCCAGGAAACACATTGCGTCAGACATTTGAGAATCAAGTGAATCGTATTCTCAATGA TGCTCGAGACAAAACTGGCTCCTCTGCACAGAAATCCCTCTCTGAATATAACAACTTCAAGTCTATGGTG GTGTCTGGAGCCAAGGGTTCCAAGATCAACATCTCCCAGGTCATTGCTGTTGTCGGGCAGCAGAATGTAG AGGGCAAGCGGATCCCATTTGGATTCAAGCATCGGACTCTTCCTCACTTTATCAAGGATGATTATGGTCC TGAGAGCCGAGGCTTTGTAGAAAATTCATACTTGGCTGGTCTCACACCCACTGAGTTCTTCTTCCATGCC ATGGGAGGACGAGAAGGTCTCATTGACACGGCTGTTAAGACTGCTGAGACTGGGTATATTCAGCGAAGGC TGATAAAGTCCATGGAGTCGGTGATGGTGAAATATGATGCAACTGTGAGGAACTCCATCAACCAGGTGGT ACAGCTGCGCTATGGAGAGGACGGCCTGGCAGGGGAGAGTGTTGAGTTCCAGAATCTGGCTACACTTAAA CCTTCTAATAAAGCTTTTGAGAAGAAGTTCCGTTTTGATTATACCAATGAGAGGGCCCTGCGGCGCACCC TTCAGGAGGACCTGGTAAAGGATGTGCTGAGCAATGCCCATATACAGAATGAGCTGGAACGGGAATTTGA ACGGATGCGTGAAGATCGGGAAGTGCTCAGGGTCATCTTCCCAACTGGTGACAGCAAGGTGGTCCTCCCT TGTAACCTTCTGCGGATGATCTGGAATGCTCAGAAAATCTTCCACATCAACCCTCGCCTTCCCTCTGACC TGCATCCCATCAAAGTGGTAGAGGGAGTCAAAGAATTGAGCAAGAAGTTGGTAATTGTGAATGGAGATGA CCCACTAAGTCGGCAGGCACAGGAGAATGCCACATTGCTCTTCAACATCCACCTAAGATCCACACTCTGC TCTCGCCGCATGGCTGAGGAGTTTCGGCTGAGTGGAGAGGCCTTTGACTGGCTGCTTGGGGAGATAGAGT CCAAGTTCAACCAAGCCATTGCCCATCCTGGAGAAATGGTGGGAGCTCTGGCTGCTCAGTCTCTTGGAGA ACCTGCCACTCAGATGACCCTGAACACTTTCCACTATGCTGGTGTATCTGCCAAGAATGTGACGCTGGGT GTGCCTCGACTTAAGGAGCTTATCAACATTTCCAAGAAGCCAAAGACTCCTTCACTCACTGTCTTCCTGT TAGGTCAATCTGCACGAGATGCTGAAAGAGCCAAGGACATTCTTTGCCGCTTGGAACATACAACATTGAG GAAGGTGACTGCCAATACAGCCATCTACTATGACCCTAACCCCCAGAGCACAGTGGTGGCAGAAGATCAG GAGTGGGTGAATGTGTACTATGAGATGCCCGACTTTGACGTGGCCCGGATATCTCCCTGGCTGCTGCGTG TGGAGCTCGACCGAAAGCACATGACTGACCGGAAGCTTACCATGGAACAGATTGCTGAGAAAATCAATGC AGGTTTTGGCGATGATTTGAATTGTATCTTCAACGATGATAATGCAGAGAAGCTGGTCCTTCGAATCCGC ATCATGAACAGTGATGAAAACAAGATGCAAGAGGAGGAAGAGGTGGTGGATAAAATGGATGATGATGTCT TCCTGCGATGCATTGAGTCCAACATGCTGACAGATATGACCCTGCAGGGTATCGAGCAGATCAGCAAGGT GTACATGCACTTACCTCAGACAGACAACAAGAAGAAGATCATCATCACAGAGGATGGGGAGTTCAAGGCC CTGCAGGAATGGATCTTAGAGACGGATGGTGTGAGCCTGATGCGGGTGCTGAGTGAGAAGGATGTAGACC CTGTGCGCACCACGTCCAATGATATTGTGGAGATCTTCACGGTACTGGGCATTGAGGCTGTGCGGAAGGC TCTGGAGCGAGAACTGTACCATGTCATCTCCTTTGATGGTTCTTATGTCAATTATCGGCATTTGGCGCTC CTGTGTGACACTATGACATGCCGTGGCCACTTGATGGCCATCACTCGTCATGGGGTCAACCGCCAGGACA CTGGACCGCTCATGAAATGCTCCTTTGAGGAAACGGTGGATGTCCTTATGGAAGCAGCAGCACATGGGGA GAGTGACCCGATGAAGGGAGTCTCTGAGAATATTATGCTGGGCCAGCTCGCTCCAGCTGGTACTGGCTGT TTTGACCTCCTGCTTGATGCTGAGAAGTGCAAATACGGCATGGAAATCCCCACCAATATCCCTGGCCTGG GGGCTGCTGGACGGAGCGGGATGACCCCAGGAGCGGCCGGCTTCTCTCCCAGTGCTGCATCTGATGCCAG TGGCTTCAGCCCAGGTTACTCCCCTGCATGGTCTCCCACACCAGGCTCTCCGGGCTCCCCTGGACCCTCA AGCCCATACATCCCCTCACCAGGTGGTGCTATGTCTCCCAGCTACTCACCGACATCACCAGCCTATGAGC CACGCTCCCCTGGGGGCTATACACCCCAGAGCCCCTCCTACTCCCCTACTTCACCTTCCTACTCCCCAAC CTCTCCATCTTACTCTCCAACCAGTCCCAACTACAGCCCTACCTCTCCTAGCTACTCGCCCACCTCCCCT AGCTACTCGCCAACCTCTCCTTCCTACTCCCCCACCTCTCCAAGCTATTCCCCAACCTCTCCTAGCTACT CCCCAACCTCTCCAAGCTATTCTCCAACATCACCTAGCTATTCTCCAACTTCTCCCAGCTACTCACCGAC ATCTCCTAGCTACTCCCCAACTTCTCCCAGCTACTCACCAACTTCACCAAGCTATTCTCCCACCTCCCCC AGCTACTCACCGACATCTCCCAGCTACTCACCAACTTCTCCAAGCTACTCACCAACTTCTCCAAGCTACT CACCCACCAGCCCTAACTATTCTCCAACTAGTCCCAACTATACCCCGACATCACCCAGCTACAGCCCAAC CTCACCCAGTTACTCACCTACAAGTCCCAACTATACACCCACCAGCCCTAATTACAGCCCAACCTCTCCA AGCTATTCCCCAACCTCACCCAGTTATTCCCCCACCTCACCAAGCTACTCCCCCTCCAGCCCACGATATA CACCACAGTCTCCAACCTACACACCAAGCTCACCAAGCTACAGTCCCAGCTCACCAAGCTACAGCCCCAC TTCACCCAAGTACACCCCAACTAGTCCTTCCTACAGTCCCAGCTCACCAGAGTACACCCCAGCTTCTCCC AAATACTCACCTACAAGCCCTAAATATTCACCCACTTCTCCCAAGTATTCTCCTACCAGCCCCACTTACT CACCTACCACCCCAAAATATTCTCCAACCTCCCCGACATACTCACCAACCTCTCCAGTCTATACCCCGAC CTCTCCCAAGTACTCCCCAACCAGCCCTACCTACTCGCCCACTTCTCCCAAGTACTCGCCCACCAGTCCC ACCTACTCACCCACCTCTCCCAAGGGCTCCACCTACTCTCCCACTTCTCCTGGCTACTCACCCACTAGCC CCACCTACAGCCTCACCAGCCCAGCCATCAGCCCAGATGACAGCGATGAGGAGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009089
Insert Size	5799 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009089.2, NP_033115.1
RefSeq Size	5799 bp
RefSeq ORF	5799 bp
Locus ID	20020
Gene Summary	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates (By similarity). Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (By similarity). Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (PubMed:26687004). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (PubMed:26687004).[UniProtKB/Swiss-Prot Function]

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