Mia3 (NM_177389) Mouse Untagged Clone

CAT#: MC225054

Mia3 (untagged) - Mouse melanoma inhibitory activity 3 (Mia3), (10ug)



  "NM_177389" in other vectors (2)

CNY 31,070.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9130229H14Rik; A930039G15Rik; AU022327; B230399H06Rik; C80126; Gm1525; Tango; TANGO1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225054 representing NM_177389
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGCGCCGGGGCTGCTCTTCTGGCTGTTCGTGCTGGGGGCGCTCTGGTGGGTCCCGGGCCAGT
CGGATCTCAGCCACGGACGGCGTTTCTCGGACCTCAAAGTGTGCGGGGACGAAGAGTGCAGCATGTTAAT
GTACCGTGGGAAAGCTCTTGAAGACTTCACGGGCCCTGATTGTCGTTTTGTGAATTTTAAAAAAGGTGAC
GATGTATATGTCTACTACAAACTGGCAGGGGGATCCCTTGAACTTTGGGCTGGAAGTGTTGAACACAGTT
TTGGATATTTTCCAAAAGATTTGATCAAGGTACTTCATAAATACACGGAAGAAGAGCTACATATTCCAGC
AGATGAGACAGACTTTGTCTGCTTTGAAGGAGGAAGAGATGATTTTAATAGTTATAATGTAGAAGAGCTT
TTAGGATCTTTGGAACTGGAGGACTCTGTACCTGAAGAGTCGAAGAAAGCTGAAGAAGTTTCTCAGCACA
GAGAGAAATCTCCTGAGGAGTCTCGGGGGCGTGAACTTGACCCTGTGCCTGAGCCCGAGGCATTCAGAGC
TGATTCAGAGGATGGAGAAGGTGCTTTCTCAGAGAGCACCGAGGGGCTGCAGGGACAGCCCTCAGCTCAG
GAGAGCCACCCTCACACCAGCGGTCCTGCGGCTAACGCTCAGGGAGTGCAGTCTTCGTTGGACACTTTTG
AAGAAATTCTGCACGATAAATTGAAAGTGCCGGGAAGCGAAAGCAGAACTGGCAATAGTTCTCCTGCCTC
GGTGGAGCGGGAGAAGACAGATGCTTACAAAGTCCTGAAAACAGAAATGAGTCTTGACCTGAAAACCAAG
TTTGGCTCAACTGCCGACGCCCTTGTGTCTGACGACGAGGCAACCAGACTGGTCACTTCATTAGAAGACG
GCTTCGATGAAGCGCTGGATGCTGAGTATTACCCGATGGAGGAGGAGGAGGAGGTAGAGGAGGATGCAGA
CAGCTCTGATGAGCTACCATTACTAACCTTCTCAGACAAAGATGAGAAAGTCCCCGGGAAGCCCATGATA
GAGAAATATTTAACAGATAAGGATCCGAATTTGAGTGAAGAGGATAAAGTTGAACCCCCTACCTGGGGGG
ATGCTTTTTTTTCTATTGTCACAGGAGGTGAAGGGAAACCAGGTGTGGTGGATTTAGAAAGATCTATCGA
GGAGGAGGAAGATGTGTCAGTTTCATCTAGCCATCAGAGGAAGCCACAGCCAGCAGCTGGCTATACAGAC
AGTGAGGATGAAGGTGACGATCTTTTTGTAGAAGAGCCCAAAACAAATGATGTAAAGGACTCAGAAACAG
ACCCAGAACTTGTCATTACAGGAGAAGAAAAGGACATCCAGGAATCCAGGAAAGGCCTGGTACAGCCTGA
GTCACAATCAGAGGATGCCAAGTCAGAAACGGCGAGCGCTTATCGTCTTCAGGGTAGCAAGCTCAACCCT
CTGTCAGCTGCTGAAAAGGGCAGAGACTTTACACTAAAAGCCGTCTTTGAAAAAAAAGAAAATGGCCTAA
AAGAATCAGTTATCCATATCTCAAAGGAAACACTACATGAAGACAAGACCAGAGAGATCCAGAGAGACAG
CTTGGAGAGTGAATTGGTACACAGAGCTTTAGGGAGTTCAGTGACAGAAAATAACAAGCCCAAATCCTTG
GGTGTTGCACCACTCTTGGGAAATAACAAGCCCGATGCATCCAAAGACAGTACAGAGGTCCCAGACGGTT
CCGTCAGTGGACCAAAAGCAGGCCAGCAGGAGGGATTTCTAGAACCAGGTCTTAAAACTCAGCACCAACC
TAGATTCTCCCCTCCAGAGGAAACTGGTCCCTCAAGAGAACTGGGGGGAAAAGTTCCTATTTCGGGAAGA
AATCTTTCTTGGCAGCAAGAGCAAGACGTAGCTGCTGTAGTCGGTAAGCATGCGAATGAGAAGACAGGGT
TTCCGGAGGAAGAGAGCAGGGAAGACGGGACAGATGCGGAGCAAGCCAGGGCCATACGAAGGCCACAGGA
GGCTGAGAGCCCGGAAGTGCTGAGTGTCCAACCTGGAAGGCCAGACGAGGAGGAGGAGGAGGAAGAGGGC
GACAACTATCCCCCCGAGGGGCTGATGGAGGATGAAAATGCTGTGAGCGCACAGCAGTCTAGAGAAAATT
CCCCCAGCGCTCGCGACGGGAGGTCAGATATGAATTCTCAAGTTTTTGAAAAAGTAATTTTGGGGACCCT
TAATCTTAACACTGAAAAAACCAAACAACCAGCTAATATGATTTTGGAGACTGGACAAGAGAGTGAAACC
ACATCCGAAGAGGCAGGTGACGTGGGCAAGGAGTCAGGTCACAGTGTGGTGGTGGACAGTGAAGAAAGCC
ATCTGGCAGATATGAGAGCCCAGAGACCCTCTCAAGTCCACGGCCTTCGTGACGAGACAGCAGCTCAGAC
TCCCGGCTCCGGGGAAGCTGTTCTGAGTAAAAATCCTAATGACCTACAAAAAGACAACCCAGAGGAAGAA
CTGGTCAACACCTTGGGACTTGAAGACCCTGGCGTAGGAGAAATCTCAGAAGGGGAGCCGGAAGATACCA
AGGAGTTTGGAGTCTCAGAAAGCCAGGGGACTGATGCTGAAGACCTTAGGGATGACCCATCCCGGCAAGC
TACACCGGAAATTCCTGACATAGTGCTCAAGAGTATAAGGGAAGACTTGCCTATCATAAACAGCTTCTTC
AAGGACGACCAACAGTCCCTGCATCGTTTCCTGAAGTACTTCGATGTTCGTGAGCTGGAGGGCTTGCTTG
AAGACATGTCGATAAGGCTGAGATCAGCGCACCAGAACAGCCTGCCGTACAACATGGAGAAAGTCCTAGA
TAAAGTCTTCCGTGCCTCTGAGTCACGAATTCTAAGTATGGCGGAGAAAATGCTTGATACGGGAGTGGCC
AAAAATAGGGACCTGGGCTCCAAAGAGAGCAGTCCGTTGGAAGAAGCCGAGGTGTTGGATGACATACAAG
ACCTGATCTATTTTGTCAGGTACCAATACTCTGGAGTGGAGACTGCCCCATTAGTCACACCTCCACCTCC
GGAGGAAGGCTGGGCCAGACCAGGAGAAGAGAGACAGCCACCCCAGCAAGACAGCTTACCACAAGAAAAC
ACAGGAGACCTCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACCAGTGTGCAGC
CTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTC
GGATCAACCTGTGACCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACTGGGTAC
ACAAGTACCTCAGAGGTATCACAGAAGCCAGATACCAAGAAAGACATAGATCTAGGTCCTGTAATGGAAG
GTGGTCCTGTTGGGGCTGGAGATGTACAAAAGCAACTAGAGACAATTGCAGAGGAGCCAGCAGCTGTCCC
ACCTCTGGAAAGTGCATTTGGTTCCCTCTATGCCTTCATCCTTTATTTAAGTAAGATGCTCCTTGCCACG
TTGCCTGACAATGTTCAGCCTGGCCCTGACTTTTACGGACTGCCCTGGCAACCTGTGATCATTACTGCAG
TCTTGGGAATTGTTTCATTTGCAATTTTCTCCTGGAGAACTATTCTTGTTGTAAAGAGTAGAGTATATCA
AGTTACTGAGAAACAAATTTCTGAAAAGTTGGAGAATATCAAGAAAGAAAATGCAGAACTTATGCAAAAA
TTGTCAAGTTATGAACAAAAGATCAAGGAGTCAAAGAAGTATGTTCAAGAAACCAAGAAACAAAACATGA
TTCTCTCCGATGAAGCTGTTAAATATAAGGATAAAATCAAGATCCTTGAAGAAACTAATGTAAGTTTGGG
TGACAAAGCCAAAAGCCTTCGTCTTCAGTTAGAGTCTGAGAGGGAGCAGAACGTCAAGAACCAGGACCTG
ATACTGGAAAACAAGAAATCTATTGAGAAGTTGAAGGATGTCATCTCAATGAATGCATCAGAACTTTCAG
AGGTTCAAGTTGCCCTTAATGAAGCCAAGCTCAGTGAAGAGAATGTAAAATCTGAGTGCCATCGGGTTCA
AGAAGAAAATGCCAGGCTTAAGAAGAAGAAAGAGCAGTTGCAGCAGCAGGTAGAAGAGTGGAGTAAATCG
CATGCCGAGCTCACTGGACAGATCAAGTCATTTGAGAAGTCCCAGGAAGACTTGGAAATAGCTCTGACTC
ACAAAGACGACAATATTAGTGCTTTGACTAATTGCATCACACAGCTGAATCGGTTAGAATGTGAACTTGA
ATCTGAGGATCCAGATAAAGGAGGAAATGAGTCAGATGATCTGGCCAATGGAGAAACGGGAGGTGACCGG
AGCGAGAAGATAAGAAATAGAATTAAGCAGATGATGGACGTCTCTCGGACACAAACTGCAGTCTCAATAG
TTGAAGAGGATCTAAAACTTTTACAGCTTAAGCTAAGAGCATCGATGTCCACTAAATGTAACCTGGAAGA
CCAAATCAAGAAATTAGAAGATGACCGCAGCTCGCTGCAGACTGCTAAAGCTGGGTTGGAAGACGAGTGC
AAAACTCTGAGGCAGAAAGTGGAGATCCTGAATGAGCTGTACCAGCAGAAGGAGATGGCGCTGCAGAAGA
AACTGAGTCAAGAAGAGTATGAGCGGCAAGACAGAGAGCAGAGGCTCACGGCGGCCGATGAGAAGGTGGT
TCTGGCTGCAGAGGAAGTGAAAACGTACAAGCGGCGAATCGAAGAAATGGAAGAAGAATTACAGAAAACA
GAACGTTCATTTAAAAACCAGATTGCTGCTCATGAGAAGAAAGCTCATGATAATTGGCTCAAAGCTCGTG
CTGCAGAGAGAGCCATGGCAGAGGAGAAGAGAGAAGCTGCTAACTTAAGGCACAAATTACTGGAAATGAC
TCAAAAGATGGCAATGAGGCAAGATGAGCCCGTGATTGTAAAGCCGATGCCAGGAAGACCGAACACACAG
AACCCTCCCCGGCGAGGTCTGCTGAGCCAGAATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGGGAAT
GCTCCCCTCCCCTACCAGCAGAGCCGCCTGGGAGACCTCTCTCTGCCACACTCAGTCGAAGAGACACTCC
TAGAAGTGAATTTGGGTCACTGGACAGGCATTTACCTCGCCCTCGATGGCCATCAGAGGCATCTGGCAAA
CACTCTGCTTCTGACCCGGGTCCCGCTCCTGTGGTGAACAGCAGCTCCAGGAGCTCTTCTCCCGCTAAGG
CCGTGGATGAGGGCAAGGTTAATATGGCTCCCAAAGGGCCGCCTCCATTTCCAGGGGTGCCTCTCATGGG
AGGCCCAGTGCCACCACCCATACGATATGGACCACCTCCCCAGCTATGTGGTGGGCCTTTTGGGCCTCGC
CCACTTCCTCCACCATTTGTTCCAGGCATGCATCCACCACTAGGCGTAAGAGAATATGCACCAGGTGTTC
TGCCTGGGAAACGGGACCTGCCTCTTGATCCTCGGGAATTTTTACCAGGACATACACCATTTAGACCTCC
AGGTTCACTCGGTCCAAGAGAGTTCTTTATTCCTGGTACCCGATTACCACCTCCAACCCATGGTCCTCAG
GAATACCCACCACCACCACCTGCTGTAAGAGACTCACTGCCTTCAGGCCCGAGAGAGGAAGCCAAACCTG
CCTCTCCAAGCAGTGTCCAGGACCGCTCACAGGCTTCAAAGCCCACCCCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_177389
Insert Size 5793 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177389.3, NP_796363.2
RefSeq Size 6933 bp
RefSeq ORF 5793 bp
Locus ID 338366
UniProt ID Q8BI84
Gene Summary Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum. Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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