Mia3 (NM_177389) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9130229H14Rik; A930039G15Rik; AU022327; B230399H06Rik; C80126; Gm1525; Tango; TANGO1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225054 representing NM_177389 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGCGCCGGGGCTGCTCTTCTGGCTGTTCGTGCTGGGGGCGCTCTGGTGGGTCCCGGGCCAGT CGGATCTCAGCCACGGACGGCGTTTCTCGGACCTCAAAGTGTGCGGGGACGAAGAGTGCAGCATGTTAAT GTACCGTGGGAAAGCTCTTGAAGACTTCACGGGCCCTGATTGTCGTTTTGTGAATTTTAAAAAAGGTGAC GATGTATATGTCTACTACAAACTGGCAGGGGGATCCCTTGAACTTTGGGCTGGAAGTGTTGAACACAGTT TTGGATATTTTCCAAAAGATTTGATCAAGGTACTTCATAAATACACGGAAGAAGAGCTACATATTCCAGC AGATGAGACAGACTTTGTCTGCTTTGAAGGAGGAAGAGATGATTTTAATAGTTATAATGTAGAAGAGCTT TTAGGATCTTTGGAACTGGAGGACTCTGTACCTGAAGAGTCGAAGAAAGCTGAAGAAGTTTCTCAGCACA GAGAGAAATCTCCTGAGGAGTCTCGGGGGCGTGAACTTGACCCTGTGCCTGAGCCCGAGGCATTCAGAGC TGATTCAGAGGATGGAGAAGGTGCTTTCTCAGAGAGCACCGAGGGGCTGCAGGGACAGCCCTCAGCTCAG GAGAGCCACCCTCACACCAGCGGTCCTGCGGCTAACGCTCAGGGAGTGCAGTCTTCGTTGGACACTTTTG AAGAAATTCTGCACGATAAATTGAAAGTGCCGGGAAGCGAAAGCAGAACTGGCAATAGTTCTCCTGCCTC GGTGGAGCGGGAGAAGACAGATGCTTACAAAGTCCTGAAAACAGAAATGAGTCTTGACCTGAAAACCAAG TTTGGCTCAACTGCCGACGCCCTTGTGTCTGACGACGAGGCAACCAGACTGGTCACTTCATTAGAAGACG GCTTCGATGAAGCGCTGGATGCTGAGTATTACCCGATGGAGGAGGAGGAGGAGGTAGAGGAGGATGCAGA CAGCTCTGATGAGCTACCATTACTAACCTTCTCAGACAAAGATGAGAAAGTCCCCGGGAAGCCCATGATA GAGAAATATTTAACAGATAAGGATCCGAATTTGAGTGAAGAGGATAAAGTTGAACCCCCTACCTGGGGGG ATGCTTTTTTTTCTATTGTCACAGGAGGTGAAGGGAAACCAGGTGTGGTGGATTTAGAAAGATCTATCGA GGAGGAGGAAGATGTGTCAGTTTCATCTAGCCATCAGAGGAAGCCACAGCCAGCAGCTGGCTATACAGAC AGTGAGGATGAAGGTGACGATCTTTTTGTAGAAGAGCCCAAAACAAATGATGTAAAGGACTCAGAAACAG ACCCAGAACTTGTCATTACAGGAGAAGAAAAGGACATCCAGGAATCCAGGAAAGGCCTGGTACAGCCTGA GTCACAATCAGAGGATGCCAAGTCAGAAACGGCGAGCGCTTATCGTCTTCAGGGTAGCAAGCTCAACCCT CTGTCAGCTGCTGAAAAGGGCAGAGACTTTACACTAAAAGCCGTCTTTGAAAAAAAAGAAAATGGCCTAA AAGAATCAGTTATCCATATCTCAAAGGAAACACTACATGAAGACAAGACCAGAGAGATCCAGAGAGACAG CTTGGAGAGTGAATTGGTACACAGAGCTTTAGGGAGTTCAGTGACAGAAAATAACAAGCCCAAATCCTTG GGTGTTGCACCACTCTTGGGAAATAACAAGCCCGATGCATCCAAAGACAGTACAGAGGTCCCAGACGGTT CCGTCAGTGGACCAAAAGCAGGCCAGCAGGAGGGATTTCTAGAACCAGGTCTTAAAACTCAGCACCAACC TAGATTCTCCCCTCCAGAGGAAACTGGTCCCTCAAGAGAACTGGGGGGAAAAGTTCCTATTTCGGGAAGA AATCTTTCTTGGCAGCAAGAGCAAGACGTAGCTGCTGTAGTCGGTAAGCATGCGAATGAGAAGACAGGGT TTCCGGAGGAAGAGAGCAGGGAAGACGGGACAGATGCGGAGCAAGCCAGGGCCATACGAAGGCCACAGGA GGCTGAGAGCCCGGAAGTGCTGAGTGTCCAACCTGGAAGGCCAGACGAGGAGGAGGAGGAGGAAGAGGGC GACAACTATCCCCCCGAGGGGCTGATGGAGGATGAAAATGCTGTGAGCGCACAGCAGTCTAGAGAAAATT CCCCCAGCGCTCGCGACGGGAGGTCAGATATGAATTCTCAAGTTTTTGAAAAAGTAATTTTGGGGACCCT TAATCTTAACACTGAAAAAACCAAACAACCAGCTAATATGATTTTGGAGACTGGACAAGAGAGTGAAACC ACATCCGAAGAGGCAGGTGACGTGGGCAAGGAGTCAGGTCACAGTGTGGTGGTGGACAGTGAAGAAAGCC ATCTGGCAGATATGAGAGCCCAGAGACCCTCTCAAGTCCACGGCCTTCGTGACGAGACAGCAGCTCAGAC TCCCGGCTCCGGGGAAGCTGTTCTGAGTAAAAATCCTAATGACCTACAAAAAGACAACCCAGAGGAAGAA CTGGTCAACACCTTGGGACTTGAAGACCCTGGCGTAGGAGAAATCTCAGAAGGGGAGCCGGAAGATACCA AGGAGTTTGGAGTCTCAGAAAGCCAGGGGACTGATGCTGAAGACCTTAGGGATGACCCATCCCGGCAAGC TACACCGGAAATTCCTGACATAGTGCTCAAGAGTATAAGGGAAGACTTGCCTATCATAAACAGCTTCTTC AAGGACGACCAACAGTCCCTGCATCGTTTCCTGAAGTACTTCGATGTTCGTGAGCTGGAGGGCTTGCTTG AAGACATGTCGATAAGGCTGAGATCAGCGCACCAGAACAGCCTGCCGTACAACATGGAGAAAGTCCTAGA TAAAGTCTTCCGTGCCTCTGAGTCACGAATTCTAAGTATGGCGGAGAAAATGCTTGATACGGGAGTGGCC AAAAATAGGGACCTGGGCTCCAAAGAGAGCAGTCCGTTGGAAGAAGCCGAGGTGTTGGATGACATACAAG ACCTGATCTATTTTGTCAGGTACCAATACTCTGGAGTGGAGACTGCCCCATTAGTCACACCTCCACCTCC GGAGGAAGGCTGGGCCAGACCAGGAGAAGAGAGACAGCCACCCCAGCAAGACAGCTTACCACAAGAAAAC ACAGGAGACCTCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACCAGTGTGCAGC CTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTC GGATCAACCTGTGACCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACTGGGTAC ACAAGTACCTCAGAGGTATCACAGAAGCCAGATACCAAGAAAGACATAGATCTAGGTCCTGTAATGGAAG GTGGTCCTGTTGGGGCTGGAGATGTACAAAAGCAACTAGAGACAATTGCAGAGGAGCCAGCAGCTGTCCC ACCTCTGGAAAGTGCATTTGGTTCCCTCTATGCCTTCATCCTTTATTTAAGTAAGATGCTCCTTGCCACG TTGCCTGACAATGTTCAGCCTGGCCCTGACTTTTACGGACTGCCCTGGCAACCTGTGATCATTACTGCAG TCTTGGGAATTGTTTCATTTGCAATTTTCTCCTGGAGAACTATTCTTGTTGTAAAGAGTAGAGTATATCA AGTTACTGAGAAACAAATTTCTGAAAAGTTGGAGAATATCAAGAAAGAAAATGCAGAACTTATGCAAAAA TTGTCAAGTTATGAACAAAAGATCAAGGAGTCAAAGAAGTATGTTCAAGAAACCAAGAAACAAAACATGA TTCTCTCCGATGAAGCTGTTAAATATAAGGATAAAATCAAGATCCTTGAAGAAACTAATGTAAGTTTGGG TGACAAAGCCAAAAGCCTTCGTCTTCAGTTAGAGTCTGAGAGGGAGCAGAACGTCAAGAACCAGGACCTG ATACTGGAAAACAAGAAATCTATTGAGAAGTTGAAGGATGTCATCTCAATGAATGCATCAGAACTTTCAG AGGTTCAAGTTGCCCTTAATGAAGCCAAGCTCAGTGAAGAGAATGTAAAATCTGAGTGCCATCGGGTTCA AGAAGAAAATGCCAGGCTTAAGAAGAAGAAAGAGCAGTTGCAGCAGCAGGTAGAAGAGTGGAGTAAATCG CATGCCGAGCTCACTGGACAGATCAAGTCATTTGAGAAGTCCCAGGAAGACTTGGAAATAGCTCTGACTC ACAAAGACGACAATATTAGTGCTTTGACTAATTGCATCACACAGCTGAATCGGTTAGAATGTGAACTTGA ATCTGAGGATCCAGATAAAGGAGGAAATGAGTCAGATGATCTGGCCAATGGAGAAACGGGAGGTGACCGG AGCGAGAAGATAAGAAATAGAATTAAGCAGATGATGGACGTCTCTCGGACACAAACTGCAGTCTCAATAG TTGAAGAGGATCTAAAACTTTTACAGCTTAAGCTAAGAGCATCGATGTCCACTAAATGTAACCTGGAAGA CCAAATCAAGAAATTAGAAGATGACCGCAGCTCGCTGCAGACTGCTAAAGCTGGGTTGGAAGACGAGTGC AAAACTCTGAGGCAGAAAGTGGAGATCCTGAATGAGCTGTACCAGCAGAAGGAGATGGCGCTGCAGAAGA AACTGAGTCAAGAAGAGTATGAGCGGCAAGACAGAGAGCAGAGGCTCACGGCGGCCGATGAGAAGGTGGT TCTGGCTGCAGAGGAAGTGAAAACGTACAAGCGGCGAATCGAAGAAATGGAAGAAGAATTACAGAAAACA GAACGTTCATTTAAAAACCAGATTGCTGCTCATGAGAAGAAAGCTCATGATAATTGGCTCAAAGCTCGTG CTGCAGAGAGAGCCATGGCAGAGGAGAAGAGAGAAGCTGCTAACTTAAGGCACAAATTACTGGAAATGAC TCAAAAGATGGCAATGAGGCAAGATGAGCCCGTGATTGTAAAGCCGATGCCAGGAAGACCGAACACACAG AACCCTCCCCGGCGAGGTCTGCTGAGCCAGAATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGGGAAT GCTCCCCTCCCCTACCAGCAGAGCCGCCTGGGAGACCTCTCTCTGCCACACTCAGTCGAAGAGACACTCC TAGAAGTGAATTTGGGTCACTGGACAGGCATTTACCTCGCCCTCGATGGCCATCAGAGGCATCTGGCAAA CACTCTGCTTCTGACCCGGGTCCCGCTCCTGTGGTGAACAGCAGCTCCAGGAGCTCTTCTCCCGCTAAGG CCGTGGATGAGGGCAAGGTTAATATGGCTCCCAAAGGGCCGCCTCCATTTCCAGGGGTGCCTCTCATGGG AGGCCCAGTGCCACCACCCATACGATATGGACCACCTCCCCAGCTATGTGGTGGGCCTTTTGGGCCTCGC CCACTTCCTCCACCATTTGTTCCAGGCATGCATCCACCACTAGGCGTAAGAGAATATGCACCAGGTGTTC TGCCTGGGAAACGGGACCTGCCTCTTGATCCTCGGGAATTTTTACCAGGACATACACCATTTAGACCTCC AGGTTCACTCGGTCCAAGAGAGTTCTTTATTCCTGGTACCCGATTACCACCTCCAACCCATGGTCCTCAG GAATACCCACCACCACCACCTGCTGTAAGAGACTCACTGCCTTCAGGCCCGAGAGAGGAAGCCAAACCTG CCTCTCCAAGCAGTGTCCAGGACCGCTCACAGGCTTCAAAGCCCACCCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177389
Insert Size	5793 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177389.3, NP_796363.2
RefSeq Size	6933 bp
RefSeq ORF	5793 bp
Locus ID	338366
UniProt ID	Q8BI84
Gene Summary	Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum. Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES.[UniProtKB/Swiss-Prot Function]

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