Myh8 (NM_177369) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4832426G23Rik; AI327267; MHCp; Myh; MyHC-; MyHC-pn; Myhs; Myhs-p; Myhsp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225063 representing NM_177369 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCGGGTTCGGACGCGGAGATGGCTATTTTTGGCGAAGCTGCTCCTTACCTCCGAAAATCGGAGA AGGAGAGGATTGAGGCCCAAAATAAGCCTTTTGACGCAAAAACATCTGTCTTTGTGGCGGAGCCCAAGGA GTCTTATGTGAAAAGCGTTATACAAAGCAAGGATGGAGGGAAAGTGACCGTGAAGACGGAAAGTGGAGCA ACTCTCACCGTCAAGGAAGATCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGG CCATGATGACCCACCTCCATGAGCCCGGAGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGAT CTATACCTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAG GTGGTGGCAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAACG CCTACCAGTTCATGCTGACGGATCGTGAGAACCAGTCCATCCTGATCACCGGAGAATCCGGGGCCGGGAA GACTGTGAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAG GAGTCTGGCAAAATGCAGGGGACCCTGGAAGATCAAATCATCAGTGCTAACCCCCTGCTGGAGGCCTTTG GGAACGCCAAGACCGTGAGGAATGACAACTCATCTCGCTTTGGCAAGTTCATCAGAATCCACTTCGGTAC CACGGGGAAACTGGCATCAGCTGATATTGAAACCTATCTTCTAGAGAAGTCTAGAGTCACTTTCCAGCTA AAAGCTGAAAGAAGTTACCACATTTTTTATCAGATCACCTCCAATAAGAAGCCAGAGCTAATTGAAATGC TCCTGATCACCACCAACCCGTATGACTACGCCTTTGTCAGTCAGGGGGAGATCACGGTGCCCAGCATTGA TGACCAAGAAGAGTTGATGGCCACAGATAGTGCCATTGATATCCTTGGCTTTTCACCTGAAGAGAAAGTG TCCATCTATAAACTCACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAAAAGCAGCGGGAGG AGCAGGCTGAGCCAGATGGCACTGAAGTGGCTGACAAGGCTGCCTATCTCCAGTGTCTGAACTCTGCTGA TCTGCTCAAAGCCCTCTGCTATCCCAGGGTCAAGGTTGGCAATGAATATGTCACCAAAGGCCAGACGGTT CAACAAGTGTATAATGCGGTGGGTGCCCTGGCCAAGGCTGTCTATGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATTGAGAAGCCACTGGGCATCTTCTCCATCCT GGAAGAAGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTACGACCAACATCTG GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTACCAAAGGCAAGGCCGAGGCCCACTTCTCCCTGGTGC ACTATGCGGGCACTGTGGATTACAACATCACCGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGACAC CGTGGTGGGGCTGTACCAGAAGTCAGCAATGAAAACTCTGGCCAGCCTCTTTTCCACATACGCTAGTGCT GAAGCAGATGGTGGTGCAAAGAAAGGAGCTAAGAAGAAGGGCTCTTCTTTCCAAACTGTGTCAGCCCTTT TCAGGGAAAACTTAAATAAACTGATGACCAATCTGAGAAGTACACACCCTCACTTCGTACGGTGTATCAT TCCCAATGAAACGAAGACTCCCGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTGAGGTGTAACGGG GTGCTGGAGGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCCGGATCCTGTATGGGGACTTTAAGCAGA GATACAAGGTTTTAAATGCAAGTGCTATTCCAGAGGGACAGTTCATCGACAGCAAGAAGGCCTCTGAGAA GCTTCTGGGCTCTATTGATATCGATCACACTCAGTATAAATTTGGACACACCAAGGTTTTCTTCAAAGCT GGTCTCCTGGGTCTCCTGGAAGAAATGAGAGATGAAAAGCTGGCCCAGATTATAACAAGAACCCAGGCGG TCTGTAGGGGATACCTAATGAGAGTGGAGTATCAGAAGATGCTGCTGAGAAGAGAATCCATCTTCTGTAT CCAGTACAACGTCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAA CCCCTGCTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGG ATGAGCTGGCCAAGTCGGAGGCAAAGCGGAAGGAGCTGGAGGAGAAGATGGTCACTCTTCTCAAAGAGAA AAATGACCTGCAACTCCAGGTTCAATCTGAAGCAGACAGCTTGGCCGATGCTGAGGAAAGGTGTGAACAA TTGATCAAAAACAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGGGCCGAGGACGAGGAGG AGATCAACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAGGACAT CGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTGAAAAAC CTGACAGAGGAGATGGCAGGCCTGGATGAAAATATCGCCAAGCTGACCAAGGAGAAGAAGGCCCTCCAGG AGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGCCAA AACCAAGCTGGAACAGCAAGTGGATGACCTTGAGGGATCGCTAGAGCAAGAAAAGAAGCTGAGGATGGAC TTAGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAGAAA ACGATAAACAGCAGCTTGATGAGAAACTCAAAAAGAAGGAGTTTGAAATCAGCAACCTGATAAGCAAAAT TGAAGATGAGCAAGCTGTGGAGATCCAGCTGCAGAAGAAGATCAAAGAGTTGCAGGCCCGCATCGAGGAG CTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCTCCC GGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGCGGGGCCACCTCTGCTCAGGTGGAGATGAA CAAGAAGCGGGAAACTGAGTTTCAGAAACTTCGCCGGGACCTGGAGGAGGCCACGCTGCAGCACGAAGCC ACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGTGTGGCTGAGCTCGGGGAGCAGATCGACAACCTGC AGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGACCTTAGCAGTAA TGCAGAGGCCATCGCCAAAGCCAAGGGAAATCTGGAGAAGATGTGCCGCACTCTGGAGGACCAGGTGAGT GAACTGAAGAGCAAGGAGGAGGAGCAGCAGCGGCTCATCAACGAGCTGACAGCGCAGAGAGCACGCCTGC AGACAGAAGCAGGTGAGTACTCCAGACAGCTGGATGAGAAAGATGCTTTAGTCTCTCAGTTGTCAAGGAG CAAGCAGGCATCCACGCAGCAGATTGAGGAGCTGAAGCGTCAGCTCGAGGAAGAAACCAAGGCCAAGAAC GCGCTGGCCCACGCCCTGCAGTCCTCCCGCCACGACTGTGATCTGCTGCGGGAACAGTATGAAGAGGAGC AGGAAGGCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAA ATATGAGACCGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGTCTG CAGGCGGCCGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGCGCTTCCCTGGAGAAGACGAAGCAGCGGC TGCAGAACGAGGTGGAGGACCTCATGATTGATGTGGAGAGGACCAACGCGGCCTGCGCCGCCCTGGACAA GAAGCAGAGAAACTTCGACAAGGTTCTATCAGAATGGAGACAGAAGTATGAGGAAACTCAGGCTGAGCTT GAGTCCTGCCAGAAGGAGTCCCGAACTCTCAGCACGGAGCTGTTCAAGGTGAAGAACGCCTACGAGGAGT CTCTGGACCACCTGGAGACGCTGAGGAGAGAGAACAAGAATCTGCAACAGGAGATCTCTGACCTGACTGA GCAGATTGCAGAGGGAGGGAAGCACATCCATGAGCTGGAGAAAATCAAGAAGCAAGTGGAACAGGAGAAG TGTGAGATACAGGCTGCCTTAGAGGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAAAGATCCTGCGCA TCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGATGAGGAGATCGA CCAGCTGAAGAGGAACCACGTTAGAGTCGTGGAGACCATGCAGAGCACGCTGGATGCCGAGATCAGGAGC AGGAATGATGCTCTGAGAGTCAAGAAGAAGATGGAGGGAGACCTGAACGAGATGGAGATCCAGCTGAACC ACGCCAACCGCTTGGCTGCTGAAAGTTTGAGGAACTACAGGAACACACAGGGCATCCTCAAGGACACCCA GCTGCATCTGGATGACGCTCTCCGGGGCCAGGAGGATCTGAAGGAGCAGCTGGCCATCGTGGAGCGCAGA GCCAACCTGCTGCAGGCTGAGATTGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGAGCAGGAAGA TTGCAGAGCAGGAGCTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAACACGAGCCTCAT CAACACCAAGAAGAAGCTGGAAAATGATGTTTCACAGCTGCAGAGTGAAGTGGAAGAAGTGATTCAAGAG TCACGCAATGCAGAAGAGAAGGCTAAGAAAGCCATTACTGATGCCGCCATGATGGCGGAGGAGCTGAAGA AGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAACAGACGGTGAAGGACCTGCA GCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAGGCC AGGGTACGTGAACTTGAAGGAGAGGTCGAAAATGAACAGAAACGCAATGCTGAGGCTGTTAAAGGGTTAA GGAAGCATGAAAGAAGAGTAAAGGAACTTACCTACCAGACTGAGGAAGACCGCAAGAATGTGCTCCGGCT GCAGGACCTGGTGGACAAATTACAGGCGAAGGTGAAATCCTACAAGAGACAGGCTGAGGAGGCTGAGGAA CAATCCAATGCCAACCTGGCCAAGTTCCGCAAGCTGCAGCACGAGCTGGAGGAAGCCGAGGAGCGGGCTG ACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGGGAGGTTCACACCAAAATCAGCGCAGA GTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177369
Insert Size	5814 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177369.3, NP_796343.2
RefSeq Size	6149 bp
RefSeq ORF	5814 bp
Locus ID	17885
UniProt ID	P13542
Gene Summary	This gene encodes a myosin heavy chain. The encoded protein forms a hexamer with two heavy chains, two alkali light chains, and two regulatory light chain components. This complex functions in muscle contraction. This gene is located in a cluster of related genes on chromosome 11. [provided by RefSeq, Jun 2013]

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