Anapc1 (NM_008569) Mouse Untagged Clone

CAT#: MC225074

Anapc1 (untagged) - Mouse anaphase promoting complex subunit 1 (Anapc1), (10ug)



  "NM_008569" in other vectors (2)

CNY 31,260.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2610021O03Rik; AI047775; AI853536; Apc1; AW547281; Mcpr; tsg24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225074 representing NM_008569
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTG
GCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGA
GTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAG
AAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCT
ATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCACTGGCAGTGTACAAAGCGTT
CACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAG
ATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCA
TAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTT
GCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATG
TTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCAT
CACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCAT
GACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAAT
GCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCAC
ATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAG
CCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTA
AGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCC
ACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGC
ACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATA
AGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCT
TTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTAT
CTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTC
CTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGAC
TTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTAC
TCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTG
AGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACA
TTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGAT
CACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCA
AAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTC
ACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATG
GACCCGAAGTTTTGACTTTGAAGGATCACTTTCCCCAGTCATTGCACCCAAAAAAGCAAGGCCTTCTGAT
ACTGGATCTGACGAGGACTGGGAGTACTTACTGAACTCAGAGTACCATCGCAATGTTGAGTCTCATCTTT
TGAACAAATCTCTATGTTTGACTGCTTTGGAAGTTTCAAATGCAAAGGATGAAGATTTTTCGCAGAACCT
CAGTCTGGATTCTTCTACCCTTCTCTTTGCTCACATTCCTGCAATTTTCTTTGTTCTTCACCTGGTTTAT
GAAGAGCTCAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCTCTTATCGACCTCCTTGTTCAGTTGG
CAAGGGACTTAAAATTGGACTCTTACTTGGACCATTACTATCGAGATTCCCCAACTCTTGTCAAAACCAC
TGGACAAGTATGCACCATTGACCAAGGTCAGATGGGATTTATGCATCATCCCCCATTTTTTACTTCTGAG
CCACCAAGTATTTATCAGTGGGTGAGTTCATGCCTGAAGGGTGAAGGGATGCCACCCTATCCTTACCTTC
CTGGGATTTGCGAGAGGAGCAGGCTAGTGGTCTTGAGCATTGCCCTCTATACCCTTGGTGATGAGAGCTG
TGTGTCTGATGAAACTTGCCAGTACTTATCCAAAGTAACCTCAACTCCCCAAAAGCCACAAGCAGAGCAA
GAAGAAAACAGGTTTACTTTCCGGCATTCTGCTTCCGTTTCTGTTCTGGCCGAGAGATTAGTTGTCTGGA
TGGCCAGTGTAGGATTCACTTTAAGAGATCTGGAGACTCTTCCCTTTGGGATTGCTCTTCCCATCAGAGA
TGCGATTTACCACTGTCGGGAGCAGCCTGATTCAGATTGGTCAGAAGCGGTCTGTCTCTTGATTGGACGT
CAGGATCTTTCCAAGCAGGCTTGTGAAGGAAATTTACCCAGAGGCAAATCTGTGCTCTCATCAGAGGTGT
CTTCAGGAACTGAGGCAGAGGAGGAAGATGATGGCATGAATGACTTGAACCATGAGGTTATGTCATTAAT
ATGGAGTGAAGATTTACGGGTGCAGGATGTGCGAAGGCTGCTTCAAAGTGCACAGCCTGTCCGTGTCAAT
GTGGTGCAGTACCCAGAACTCAGTGACCATGAGTTCATTGAAGAAAAGGAAAACAGACTGCTCCAGTTGT
GTCAGCGAACTATGGCCCTTCCAGTAGGACGAGGAATGTTTACCTTGTTCTCATATCATCCTGTTCCAAC
AGAGCCGTTGCCTGTTCCTAAATTGAATCTGACAGGGCGAGCCCCTCCACGAAACACAACTGTAGATCTT
AATAGCGGAAACATCGACGTGCCTCCCAACATGGCCAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCCG
GCCTGAAGATAGCCCCAGCCTCCCAGATAGACTCAGCTTGGATCGTTTACAACAAGCCAAAGCATGCTGA
GTTAGCCAATGAGTATGCCGGCTTTCTCATGGCCCTGGGTCTGAATGGGCACCTTACCAAGCTGGCTACT
CTCAATATCCATGACTATTTGACCAAGGGCCATGAAATGACAAGCATTGGATTGCTACTTGGTGTTTCTG
CTGCAAAGCTTGGCACCATGGACATGTCAATTACCCGGCTCCTCAGCATTCACGTTCCTGCTCTCTTACC
CCCGACATCCACAGAGCTTGACGTGCCTCACAATGTCCAAGTGGCGGCTGTGGTTGGCATTGGCCTTGTG
TATCAGGGCACAGCTCACAGACACACTGCGGAAGTCCTGTTGGCTGAAATAGGGCGGCCCCCTGGTCCAG
AAATGGAATACTGCACTGACAGAGAGTCGTACTCCTTAGCTGCTGGCCTGGCCCTGGGCATGGTTTGCTT
GGGGCATGGCAGCAATTTGATTGGCATGTCTGATCTCAATGTGCCTGAGCAGCTGTATCAGTACATGGTT
GGAGGCCATAGGCGATTTCAAACTGGAATGCACAGGGAGAAACATAAGTCTCCAAGTTATCAGATCAAAG
AAGGAGACACCATAAACGTGGATGTGACTTGTCCCGGTGCTACTCTGGCGTTGGCTATGATCTACTTAAA
AACCAATAACAGGTCCATTGCTGATTGGCTGCGTGCTCCTGATACCATGTATTTGCTAGACTTTGTGAAA
CCAGAATTCCTTTTGCTTAGGACACTTGCTCGGTGCTTGATTTTGTGGGATGATATCTTACCAAATTCCA
AGTGGGTTGACAGCAATGTTCCTCAGATTATAAGAGAAAATAGTATCTCTCTGAGTGAAATTGAATTGCC
TTGTTCAGAGGACTTGAATTTGGAAACCTTGTCGCAAGCACACGTCTACATCATTGCAGGAGCCTGCTTG
TCTCTAGGGTTTCGATTTGCTGGCTCAGAAAACTTATCAGCATTTAGCTGTCTGCATAAATTTGCAAAAG
ATTTTATGAATTATTTATCTGCACCCAATGCTTCTGTAACAGGGCCCTATAACCTCGAAACCTGCCTGAG
TGTGGTCCTGCTGTCTCTTGCCATGGTGATGGCTGGCTCTGGGAACCTGAAGGTGTTGCAGCTCTGTCGC
TTTCTGCACATGAAGACTGGTGGAGAGATGAACTATGGCTTCCACTTGGCCCACCACATGGCCCTGGGCC
TTCTCTTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCCAACTCATCCATTGCTGCCCTTCTCTGCGC
CCTTTACCCACATTTCCCAGCCCACAGCACTGACAACCGGTATCATCTCCAGGCCCTTCGGCACCTCTAT
GTGCTAGCTGCAGAACCAAGGCTCCTGGTACCTGTGGATGTGGATACAAACACACCCTGCTATGCCCTTA
TAGAAGTTACTTACAAGGGCACTCAGTGGTATGAACAGACCAAAGAAGAACTGATGGCTCCAACCCTTCT
TCCAGAACTTCATCTTTTAAAGCAGATGAAAGTTAAAGGGCCAAGATACTGGGAACTGCTCATAGATTTA
AGCAAGGGAGAACAGCACTTGAGGTCTATTCTTTCGAAGGATGGAGTTTTATATGTAAAGCTCAGGGCAG
GACAGCTCTCCTACAAAGAAGACCCAATGGGGTGGCAGAGTTTGTTGGCACAAACTGTTGCTAATAGGAA
CTCTGAAGCCCGGGCTTTCAAGCCTGAAACAATTTCATCATTCACTTCTGATCCAGCACTTCTGTCATTT
GCTGAATATTTCTGCAAGCCGACTGTGAGCATGGGTCCAAAACAGGAGATTCTGGACCTCTTTTCTTCGA
TACTTTATGAGTGTGTTGCTCAGGAAACCCCAGAGATGTTGCCTGCCTACATAGCAATGGATCAGGCTCT
AAGAAGTCTTAAAAAGAGAGACATGTCAGACACATCTGATCTTTGGCAGATAAAGTTGATATTAGAATTT
TTCAGTTCCCGAAGCCACCAGGATAGGCAGCATACCTATCCTAAGCGAGGGCTCTTCATAAATTCTGAGT
TCCTACCTGTGGTGAAGTGCACTGTTGATGCTACCCTGGACCAGTGGCTACAAGCTGGCGGTGATGTGTG
TGTGCATGCCTACCTCAGTGGGCAGCCTGTTGAGAAGTCTCAGCTAAACATGCTGGCCTGCTTCCTTGTC
TACCACTCTGTGCCAGCACCACGACACTTGCCCCCCATGGGACTGGAAGGGAGCACAAGCTTTGCTGAAC
TCCTCTACAGGTTCCGACACCTGAAGATGCCCGTCCGGGCTTTGCTAAGACTGGCTCCTGTGCTGCTTGG
GAACCCACAGCCGATGGTCATGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_008569
Insert Size 5835 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008569.2, NP_032595.2
RefSeq Size 8967 bp
RefSeq ORF 5835 bp
Locus ID 17222
UniProt ID P53995
Gene Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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