Anapc1 (NM_008569) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610021O03Rik; AI047775; AI853536; Apc1; AW547281; Mcpr; tsg24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225074 representing NM_008569 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTG GCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGA GTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAG AAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCT ATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCACTGGCAGTGTACAAAGCGTT CACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAG ATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCA TAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTT GCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATG TTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCAT CACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCAT GACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAAT GCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCAC ATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAG CCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTA AGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCC ACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGC ACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATA AGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCT TTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTAT CTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTC CTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGAC TTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTAC TCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTG AGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACA TTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGAT CACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCA AAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTC ACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATG GACCCGAAGTTTTGACTTTGAAGGATCACTTTCCCCAGTCATTGCACCCAAAAAAGCAAGGCCTTCTGAT ACTGGATCTGACGAGGACTGGGAGTACTTACTGAACTCAGAGTACCATCGCAATGTTGAGTCTCATCTTT TGAACAAATCTCTATGTTTGACTGCTTTGGAAGTTTCAAATGCAAAGGATGAAGATTTTTCGCAGAACCT CAGTCTGGATTCTTCTACCCTTCTCTTTGCTCACATTCCTGCAATTTTCTTTGTTCTTCACCTGGTTTAT GAAGAGCTCAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCTCTTATCGACCTCCTTGTTCAGTTGG CAAGGGACTTAAAATTGGACTCTTACTTGGACCATTACTATCGAGATTCCCCAACTCTTGTCAAAACCAC TGGACAAGTATGCACCATTGACCAAGGTCAGATGGGATTTATGCATCATCCCCCATTTTTTACTTCTGAG CCACCAAGTATTTATCAGTGGGTGAGTTCATGCCTGAAGGGTGAAGGGATGCCACCCTATCCTTACCTTC CTGGGATTTGCGAGAGGAGCAGGCTAGTGGTCTTGAGCATTGCCCTCTATACCCTTGGTGATGAGAGCTG TGTGTCTGATGAAACTTGCCAGTACTTATCCAAAGTAACCTCAACTCCCCAAAAGCCACAAGCAGAGCAA GAAGAAAACAGGTTTACTTTCCGGCATTCTGCTTCCGTTTCTGTTCTGGCCGAGAGATTAGTTGTCTGGA TGGCCAGTGTAGGATTCACTTTAAGAGATCTGGAGACTCTTCCCTTTGGGATTGCTCTTCCCATCAGAGA TGCGATTTACCACTGTCGGGAGCAGCCTGATTCAGATTGGTCAGAAGCGGTCTGTCTCTTGATTGGACGT CAGGATCTTTCCAAGCAGGCTTGTGAAGGAAATTTACCCAGAGGCAAATCTGTGCTCTCATCAGAGGTGT CTTCAGGAACTGAGGCAGAGGAGGAAGATGATGGCATGAATGACTTGAACCATGAGGTTATGTCATTAAT ATGGAGTGAAGATTTACGGGTGCAGGATGTGCGAAGGCTGCTTCAAAGTGCACAGCCTGTCCGTGTCAAT GTGGTGCAGTACCCAGAACTCAGTGACCATGAGTTCATTGAAGAAAAGGAAAACAGACTGCTCCAGTTGT GTCAGCGAACTATGGCCCTTCCAGTAGGACGAGGAATGTTTACCTTGTTCTCATATCATCCTGTTCCAAC AGAGCCGTTGCCTGTTCCTAAATTGAATCTGACAGGGCGAGCCCCTCCACGAAACACAACTGTAGATCTT AATAGCGGAAACATCGACGTGCCTCCCAACATGGCCAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCCG GCCTGAAGATAGCCCCAGCCTCCCAGATAGACTCAGCTTGGATCGTTTACAACAAGCCAAAGCATGCTGA GTTAGCCAATGAGTATGCCGGCTTTCTCATGGCCCTGGGTCTGAATGGGCACCTTACCAAGCTGGCTACT CTCAATATCCATGACTATTTGACCAAGGGCCATGAAATGACAAGCATTGGATTGCTACTTGGTGTTTCTG CTGCAAAGCTTGGCACCATGGACATGTCAATTACCCGGCTCCTCAGCATTCACGTTCCTGCTCTCTTACC CCCGACATCCACAGAGCTTGACGTGCCTCACAATGTCCAAGTGGCGGCTGTGGTTGGCATTGGCCTTGTG TATCAGGGCACAGCTCACAGACACACTGCGGAAGTCCTGTTGGCTGAAATAGGGCGGCCCCCTGGTCCAG AAATGGAATACTGCACTGACAGAGAGTCGTACTCCTTAGCTGCTGGCCTGGCCCTGGGCATGGTTTGCTT GGGGCATGGCAGCAATTTGATTGGCATGTCTGATCTCAATGTGCCTGAGCAGCTGTATCAGTACATGGTT GGAGGCCATAGGCGATTTCAAACTGGAATGCACAGGGAGAAACATAAGTCTCCAAGTTATCAGATCAAAG AAGGAGACACCATAAACGTGGATGTGACTTGTCCCGGTGCTACTCTGGCGTTGGCTATGATCTACTTAAA AACCAATAACAGGTCCATTGCTGATTGGCTGCGTGCTCCTGATACCATGTATTTGCTAGACTTTGTGAAA CCAGAATTCCTTTTGCTTAGGACACTTGCTCGGTGCTTGATTTTGTGGGATGATATCTTACCAAATTCCA AGTGGGTTGACAGCAATGTTCCTCAGATTATAAGAGAAAATAGTATCTCTCTGAGTGAAATTGAATTGCC TTGTTCAGAGGACTTGAATTTGGAAACCTTGTCGCAAGCACACGTCTACATCATTGCAGGAGCCTGCTTG TCTCTAGGGTTTCGATTTGCTGGCTCAGAAAACTTATCAGCATTTAGCTGTCTGCATAAATTTGCAAAAG ATTTTATGAATTATTTATCTGCACCCAATGCTTCTGTAACAGGGCCCTATAACCTCGAAACCTGCCTGAG TGTGGTCCTGCTGTCTCTTGCCATGGTGATGGCTGGCTCTGGGAACCTGAAGGTGTTGCAGCTCTGTCGC TTTCTGCACATGAAGACTGGTGGAGAGATGAACTATGGCTTCCACTTGGCCCACCACATGGCCCTGGGCC TTCTCTTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCCAACTCATCCATTGCTGCCCTTCTCTGCGC CCTTTACCCACATTTCCCAGCCCACAGCACTGACAACCGGTATCATCTCCAGGCCCTTCGGCACCTCTAT GTGCTAGCTGCAGAACCAAGGCTCCTGGTACCTGTGGATGTGGATACAAACACACCCTGCTATGCCCTTA TAGAAGTTACTTACAAGGGCACTCAGTGGTATGAACAGACCAAAGAAGAACTGATGGCTCCAACCCTTCT TCCAGAACTTCATCTTTTAAAGCAGATGAAAGTTAAAGGGCCAAGATACTGGGAACTGCTCATAGATTTA AGCAAGGGAGAACAGCACTTGAGGTCTATTCTTTCGAAGGATGGAGTTTTATATGTAAAGCTCAGGGCAG GACAGCTCTCCTACAAAGAAGACCCAATGGGGTGGCAGAGTTTGTTGGCACAAACTGTTGCTAATAGGAA CTCTGAAGCCCGGGCTTTCAAGCCTGAAACAATTTCATCATTCACTTCTGATCCAGCACTTCTGTCATTT GCTGAATATTTCTGCAAGCCGACTGTGAGCATGGGTCCAAAACAGGAGATTCTGGACCTCTTTTCTTCGA TACTTTATGAGTGTGTTGCTCAGGAAACCCCAGAGATGTTGCCTGCCTACATAGCAATGGATCAGGCTCT AAGAAGTCTTAAAAAGAGAGACATGTCAGACACATCTGATCTTTGGCAGATAAAGTTGATATTAGAATTT TTCAGTTCCCGAAGCCACCAGGATAGGCAGCATACCTATCCTAAGCGAGGGCTCTTCATAAATTCTGAGT TCCTACCTGTGGTGAAGTGCACTGTTGATGCTACCCTGGACCAGTGGCTACAAGCTGGCGGTGATGTGTG TGTGCATGCCTACCTCAGTGGGCAGCCTGTTGAGAAGTCTCAGCTAAACATGCTGGCCTGCTTCCTTGTC TACCACTCTGTGCCAGCACCACGACACTTGCCCCCCATGGGACTGGAAGGGAGCACAAGCTTTGCTGAAC TCCTCTACAGGTTCCGACACCTGAAGATGCCCGTCCGGGCTTTGCTAAGACTGGCTCCTGTGCTGCTTGG GAACCCACAGCCGATGGTCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_008569
Insert Size	5835 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008569.2, NP_032595.2
RefSeq Size	8967 bp
RefSeq ORF	5835 bp
Locus ID	17222
UniProt ID	P53995
Gene Summary	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]

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