Cacna1f (NM_019582) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A930034B14; Cav1.4; nerg1; nob2; Sfc17
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225104 representing NM_019582 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAATCTGAAGTCGGGAAAGATACAACCCCAGAGCCCAGTCCAGCCAATGGGACTGGCCCTGGCC CTGAATGGGGGCTCTGTCCTGGGCCTCCAACTGTGGGGACTGATACCAGCGGGGCGTCAGGCCTGGGGAC CCCAAGAAGAAGGACCCAGCACAACAAACACAAGACTGTGGCGGTGGCCAGTGCTCAGAGATCACCTCGA GCGCTCTTCTGCCTCACCCTTACTAATCCCATTCGTCGGTCCTGCATCAGCATTGTAGAGTGGAAGCCTT TTGATATTCTCATCCTCCTGACAATCTTTGCCAACTGCGTGGCATTGGGGGTATATATCCCCTTCCCTGA GGACGACTCCAACACTGCTAACCACAACTTGGAACAGGTAGAATACGTGTTCCTGGTGATTTTCACCGTG GAGACAGTGCTCAAGATCGTAGCCTATGGGCTGGTGCTCCATCCCAGCGCCTATATTCGCAATGGCTGGA ACCTGCTCGACTTCATCATCGTCGTGGTCGGGCTGTTCAGCGTGCTGCTGGAACAAGGACCTGGGCGGCC AGGAGATGCCCCGCATACTGGAGGAAAGCCAGGAGGCTTCGATGTAAAGGCACTGCGGGCATTTAGGGTG CTACGACCTCTAAGGCTAGTGTCTGGGGTCCCGAGTCTGCACATAGTGCTCAATTCCATCATGAAGGCGC TTGTGCCGCTGCTGCACATTGCCCTGTTGGTGCTCTTCGTCATTATCATTTACGCCATCATCGGACTCGA GCTATTCCTCGGACGAATGCACAAGACATGCTACTTCCTGGGATCTGATATGGAAGCAGAGGAGGACCCA TCACCTTGTGCATCTTCTGGCTCTGGGCGTTCATGCACACTGAACCATACCGAGTGCCGCGGGCGCTGGC CAGGACCCAACGGTGGCATCACGAACTTCGACAATTTTTTCTTTGCCATGCTAACTGTGTTCCAGTGTAT TACCATGGAAGGCTGGACAGACGTCCTCTACTGGATGCAGGATGCCATGGGGTATGAGCTGCCTTGGGTG TACTTTGTGAGCCTTGTCATCTTTGGGTCCTTCTTTGTCCTCAACCTTGTGCTTGGAGTCCTAAGCGGGG AGTTCTCCAAGGAAAGAGAAAAGGCAAAAGCACGAGGTGACTTTCAGAAGCTTCGGGAGAAGCAGCAGAT GGAAGAAGACCTTCGGGGCTACCTGGACTGGATCACACAGGCTGAGGAGTTAGACCTTCATGACCCCTCA GTAGACGGCAACTTGGCTTCTCTTGCTGAAGAGGGACGGGCGGGCCATCGGCCACAACTGTCAGAGCTGA CCAATAGGAGGCGCGGACGGCTGCGATGGTTCAGCCACTCTACTCGCTCCACACACTCCACCAGCAGCCA CGCCAGCCTCCCAGCCAGTGACACTGGCTCCATGACAGACACCCCTGGAGATGAGGATGAAGAAGAGGGG ACCATGGCTAGCTGTACACGCTGCCTAAACAAGATTATGAAAACAAGGATCTGCCGCCACTTCCGCCGAG CCAACCGGGGTCTCCGTGCACGCTGCCGCCGGGCCGTCAAGTCCAACGCCTGCTACTGGGCTGTACTGTT GCTCGTCTTCCTCAACACGTTGACCATAGCTTCAGAGCACCATGGGCAGCCTTTGTGGCTCACCCAGACC CAAGAGTATGCCAACAAAGTTCTGCTCTGCCTCTTCACTGTGGAGATGCTCCTCAAACTGTACGGCCTGG GCCCCTCTGTCTACGTTGCCTCCTTTTTCAACCGCTTTGACTGCTTCGTGGTCTGTGGGGGCATCCTAGA AACCACTTTGGTGGAGGTGGGGGCCATGCAGCCTCTTGGCATCTCAGTGCTCCGATGTGTACGTCTCCTC AGGATCTTCAAGGTCACCAGGCACTGGGCATCCCTGAGCAATCTGGTGGCATCTTTGCTCAATTCCATGA AGTCCATCGCCTCCTTGCTGCTTCTCCTCTTTCTCTTCATCATCATCTTCTCCCTGCTTGGCATGCAGCT GTTTGGGGGCAAGTTCAACTTTGACCAGACCCACACCAAGAGGAGCACCTTTGATACCTTCCCCCAAGCC CTCCTCACTGTCTTTCAGATCCTGACTGGTGAGGATTGGAACGTTGTCATGTATGATGGTATCATGGCCT ACGGTGGGCCCTTCTTCCCAGGGATGCTGGTGTGTGTTTATTTCATCATCCTCTTCATCTGTGGCAACTA CATCCTGCTGAACGTGTTTCTTGCCATTGCCGTGGATAACCTAGCCAGCGGGGATGCAGGCACTGCCAAA GACAAGGGCAGAGAGAAGAGCAGTGAAGGAAACCCTCCAAAGGAGAACAAAGTATTGGTGCCTGGTGGAG AGAATGAGGACGCAAAGGGTGCAAGAAGTGAAGGAGCAGCACCAGGCATGGAGGAGGAGGAGGAGGAGGA AGAAGAAGAAGAGGAGGAGGAGGAAGAGGAAAATGGTGCAGGACATGTGGAACTCTTGCAGGAAGTAGTA CCCAAGGAGAAGGTGGTACCCATCCCTGAAGGCAGTGCCTTCTTCTGCCTTAGCCAAACCAACCCGCTTC GGAAGGCCTGCCACACACTCATACATCACCATATCTTCACCAGTCTCATCCTAGTGTTCATCATCCTCAG TAGTGTGTCCCTGGCTGCTGAGGACCCCATCCGAGCTCACTCCTTCCGAAACCATATTCTGGGATATTTT GATTATGCCTTCACCTCCATATTCACTGTGGAGATTCTACTCAAGATGACAGTGTTTGGGGCCTTCCTGC ACCGAGGCTCTTTCTGCCGTAGCTGGTTCAATCTGTTGGATCTCCTTGTGGTCAGTGTGTCCCTCATCTC CTTCGGCATCCACTCCAGTGCCATCTCAGTTGTGAAGATTCTCCGAGTCCTCCGAGTCCTGCGGCCTCTC CGAGCCATCAACAGAGCCAAGGGACTCAAGCATGTGGTGCAGTGTGTGTTCGTGGCCATCCGGACCATCG GAAACATCATGATTGTCACCACCCTCTTGCAGTTCATGTTCGCCTGCATTGGTGTTCAGCTGTTCAAGGG AAAATTCTACAGTTGCACTGATGAGGCCAAACACACCCTGAAAGAATGCAAGGGCTCCTTCCTCATCTAC CCTGATGGAGATGTGTCACGACCTTTGGTCCGGGAGCGGCTCTGGGTCAACAGTGATTTTAACTTTGACA ACGTCCTTTCAGCCATGATGGCCCTGTTCACTGTCTCTACCTTTGAAGGCTGGCCTGCGCTACTATACAA GGCCATAGATGCAAACGCAGAAGATGAGGGCCCTATCTACAATTACCATGTGGAGATATCAGTATTCTTC ATTGTCTACATCATCATCATCGCCTTCTTCATGATGAACATCTTTGTGGGCTTTGTTATCATCACATTCC GTGCCCAGGGAGAGCAGGAGTACCAAAACTGTGAACTGGACAAGAACCAGCGCCAGTGTGTGGAATATGC CCTCAAAGCTCAGCCACTCCGCCGATACATCCCTAAGAATCCTCATCAGTACCGCGTGTGGGCCACTGTG AACTCTGCTGCCTTTGAGTACCTCATGTTTCTGCTCATCCTGCTCAACACGGTGGCCCTAGCCATGCAGC ACTATGAACAGACTGCTCCCTTTAACTATGCCATGGACATCCTCAACATGGTCTTCACTGGCCTCTTCAC CATTGAGATGGTGCTCAAAATCATCGCCTTTAAACCCAAGCATTACTTTGCAGATGCCTGGAATACGTTT GATGCTCTCATTGTAGTGGGCAGTGTAGTCGACATCGCCGTCACAGAAGTCAATAACGGAGGCCATCTTG GCGAGAGTTCAGAGGACAGCTCCCGCATATCTATCACGTTCTTTCGCCTCTTCCGAGTCATGAGGCTGGT CAAGCTTCTGAGTAAGGGTGAGGGGATCCGCACACTGCTCTGGACATTCATCAAGTCTTTCCAGGCCTTG CCCTATGTGGCACTTCTCATAGCAATGATATTCTTCATCTATGCAGTCATTGGCATGCAGATGTTTGGCA AGGTGGCTCTTCAGGACGGCACGCAGATAAATCGAAACAACAATTTCCAGACCTTTCCGCAGGCTGTGCT GCTTCTGTTCAGGTGTGCCACTGGTGAGGCCTGGCAAGAGATAATGCTAGCCAGCCTTCCAGGAAATCGA TGTGACCCTGAGTCTGACTTTGGCCCAGGCGAGGAATTTACCTGTGGTAGCAGTTTTGCCATCGTCTACT TCATCAGCTTCTTTATGCTCTGTGCCTTCCTGATTATAAATCTCTTTGTGGCTGTAATCATGGATAACTT TGATTACCTAACCAGAGATTGGTCTATCCTGGGACCCCACCACCTTGATGAATTCAAGAGGATCTGGTCT GAATATGACCCCGGAGCCAAGGGCCGCATCAAGCACTTGGATGTGGTTGCCCTGCTGAGACGCATCCAGC CCCCATTGGGATTTGGAAAGCTATGCCCACACCGAGTGGCCTGCAAGAGACTCGTGGCAATGAATGTGCC CCTCAACTCAGATGGAACAGTGACATTCAACGCTACACTCTTTGCCCTGGTGCGGACATCCCTGAAGATC AAGACAGAAGGGAACCTGGATCAAGCCAACCAGGAGCTTCGGATGGTCATCAAAAAGATCTGGAAGCGGA TAAAGCAGAAATTGTTGGATGAGGTCATCCCTCCTCCCGATGAGGAGGAGGTCACTGTGGGAAAATTCTA TGCCACATTCCTGATCCAAGATTATTTCCGAAAATTCCGGAGAAGGAAAGAAAAGGGGCTACTAGGAAGA GAGGCCCCAACAAGCACATCCTCTGCCCTCCAGGCTGGTCTAAGGAGCCTGCAGGACTTGGGTCCTGAGA TCCGTCAAGCCCTCACCTATGACACTGAGGAAGAAGAGGAAGAGGAAGAGGCAGTGGGTCAGGAGGCTGA GGAAGAGGAAGCTGAGAACAACCCAGAACCATACAAAGACTCCATAGACTCCCAGCCCCAATCTCGATGG AACTCTAGGATTTCGGTGTCTCTACCTGTTAAGGAGAAACTTCCAGATTCTCTCTCAACTGGGCCGAGTG ATGATGATGGGCTGGCTCCCAACTCCAGGCAGCCCAGTGTGATACAGGCTGGCTCCCAACCACACAGGAG AAGCTCTGGGGTTTTCATGTTCACTATCCCGGAAGAAGGAAGTATTCAGCTCAAGGGAACTCAAGGGCAG GACAATCAGAATGAGGAACAGGAAGTCCCTGACTGGACTCCTGACCTGGATGAGCAGGCCGGGACTCCTT CGAACCCAGTCCTTTTACCACCTCACTGGTCCCAGCAACACGTAAACGGGCACCATGTGCCACGCCGACG TTTGCTGCCCCCCACGCCTGCAGGTCGGAAGCCCTCCTTCACCATCCAGTGTCTGCAACGCCAGGGCAGT TGTGAAGATTTACCTATCCCAGGCACCTACCATCGTGGACGGACCTCAGGACCAAGCAGGGCTCAGGGTT CCTGGGCAGCCCCTCCTCAGAAGGGTCGACTGCTATATGCCCCCCTGTTGTTGGTGGAGGAATCTACAGT GGGTGAAGGATACCTTGGCAAACTTGGCGGCCCACTGCGTACCTTCACCTGTCTGCAAGTGCCTGGAGCT CATCCGAATCCCAGCCACCGCAAGAGGGGCAGTGCTGACAGTTTGGTGGAGGCTGTGCTCATCTCCGAAG GCCTAGGTCTCTTTGCCCAAGACCCACGATTTGTGGCCCTGGCCAAGCAGGAGATTGCAGATGCATGTCA CCTGACCCTGGATGAGATGGACAGTGCTGCCAGTGACCTGCTGGCACAGAGAACCACCTCCCTTTACAGT GATGAGGAGTCTATTCTTTCCCGCTTTGATGAAGAGGACCTGGGAGATGAGATGGCCTGTGTCCATGCCC TCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019582
Insert Size	5955 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019582.2, NP_062528.2
RefSeq Size	6075 bp
RefSeq ORF	5955 bp
Locus ID	54652

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