Cacna1h (NM_001163691) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	alpha13.2; Cav3; Cav3.2; MNCb-1209
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225116 representing NM_001163691 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGAGGGCACGCTGGCAGCGGACGAGGTCCGGGTGCCCCTGGGCGCTTCGCCTTCGGCTCCTGCAG CGCCGGTGAGGGCTTCCCCAGCGAGCCCTGGGGTGCCGGGGCGCGAGGAGCAGCGAGGATCCGGGTCGAG CGTGTTGGCTCCCGAGAGCCCAGGGACCGAGTGTGGTGCGGACCTGGGCGCCGACGAGGAACAGCCCGTC CCATACCCCGCTCTGGCTGCCACAGTCTTCTTCTGCCTCGGGCAAACCACGCGGCCCCGCAGCTGGTGCC TCCGACTGGTTTGTAACCCGTGGTTCGAGCACATTAGCATGCTGGTCATCATGCTGAACTGCGTGACACT GGGCATGTTCCGGCCCTGTGAGGATGTTGAGTGCCGTTCAGAACGTTGCAGCATCTTGGAGGCCTTTGAT GACTTCATCTTTGCCTTCTTTGCTGTGGAGATGGTTATCAAGATGGTGGCTTTGGGACTGTTCGGCCAGA AATGCTACCTGGGTGACACCTGGAACAGGCTGGACTTCTTCATTGTCATGGCTGGCATGATGGAATACTC TCTGGACGGACACAACGTGAGCCTCTCTGCCATCCGAACAGTGCGTGTGCTGCGGCCCCTCCGCGCCATC AACCGGGTCCCCAGCATGCGAATCCTGGTCACACTGCTGCTGGACACACTGCCCATGCTTGGGAACGTGC TTCTTCTCTGCTTCTTCGTCTTCTTCATCTTCGGCATCGTTGGGGTCCAGCTCTGGGCTGGCCTGCTGCG GAACCGATGCTTCCTGGACAGCGCCTTCGTCAGGAACAACAACCTGACCTTCTTGCGGCCATACTACCAG ACAGAGGAGGGCGAGGAGAACCCATTCATCTGCTCCTCACGCCGCGACAACGGCATGCAGAAATGCTCAC ACATCCCCAGCCGCCGTGAGCTTCGCGTGCAGTGCACGCTCGGCTGGGAGGCCTACGGGCAGCCACAGGC TGAGGATGGGGGTGCCGGCCGAAATGCCTGCATCAACTGGAACCAGTACTACAACGTGTGCCGCTCGGGG GAATTCAACCCTCACAATGGTGCCATCAACTTTGACAACATTGGCTATGCTTGGATCGCCATCTTCCAGG TCATCACACTGGAGGGCTGGGTGGACATCATGTACTACGTCATGGATGCCCACTCGTTCTACAACTTCAT CTACTTCATCCTCCTCATCATTGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATAGCCACA CAGTTCTCAGAGACAAAGCAGAGGGAAAACCAGCTGATGCGTGAACAGCGGGCCCGCTATCTGTCCAACG ACAGCACTCTGGCCAGCTTCTCGGAGCCTGGCAGCTGCTACGAGGAGCTCCTCAAGTATGTGGGCCACAT CTTCCGAAAGGTTAAACGCCGGAGCCTGCGCCTTTACGCCCGCTGGCAGAGCCGTTGGCGTAAGAAGGTA GATCCCAGCAGCACTCTGCATGGCCAAGGCCCCAGGCGGCGGCCACGGCGCGCAGGCAGGCGCACAGCTT CTGTGCACCATCTGGTCTACCACCACCACCACCACCATCACCACCATTACCACTTCAGCCACGGTGGCCC ACGCAGGCCTAGCCCAGAGCCAGGTGCTGGTGACACCAGGTTGGTCAGGGCCTGTGTGCCACCCTCGCCA CCATCCCCAGGCCACGGGCCACCAGACTCTGAGTCTGTGCACAGTATCTACCATGCTGACTGCCACGTGG AGGGGCCACAGGAAAGAGCCCGTGTGGCGCACACCATAGCCACTGCTGCCAGCCTCAAGCTGGCCTCAGG CTTGGGCACCATGAACTACCCCACCATCCTACCTTCAGGAGCAGTCAACAGCAAAGGCAGTACCAGCTCG CGACCCAAGGGGCTTCGGAGTGCTGGCACCCCAGGGGCCACAGCACACAGCCCTCTGAGCCTGGGGAGCC CCAGCCCCTATGAGAAGATCCAGCACGTGGTTGGAGAACAAGGACTAGGCCGAGCCTCTAGTCACCTGTC AGGCCTGAGTGTGCCTTGCCCCCTGCCCAGCCCCCAGGCTGGCACACTGACCTGTGAGCTGAAGAGCTGC CCATATTGTGCCAGCGCCCTGGAGGACCCCGAGTTTGAGTTCAGTGGCTCAGAGAGTGGGGACTCGGATG CCCATGGAGTCTATGAGTTTACACAGGACGTACGGCATGGGGATTGCCGAGATCCTGTGCAGCAGCCCCA TGAAGGGGGCACGCCGGGCCATGGCAACGAACGGTGGCGGCCACCACTGCGGACAGCCTCACAGCCAGGA GGGTTAGGCCGCCTCTGGGCTTCCTTTAGTAGCAAGCTGCGTCGCATTGTAGACAGCAAGTACTTCAACC GAGGCATCATGGCAGCCATCCTCGTCAATACTCTGAGCATGGGTGTTGAGTATCATGAGCAGCCTGATGA GCTGACTAACGCGCTGGAGATAAGCAACATCGTGTTCACCAGCATGTTTGCCTTGGAGATGCTACTGAAG TTGCTGGCCTGTGGCCCGCTGGGCTACATTCGGAACCCCTACAACATCTTTGATGGCATTGTTGTCATCA TAAGCGTCTGGGAGATTGTGGGGCAGGCAGATGGTGGCCTGTCAGTGCTCCGTACGTTCCGGCTGCTGCG GGTGCTGAAGCTGGTGCGCTTCCTGCCAGCCCTGCGGCGGCAGCTGGTGGTGCTCATGAGGACCATGGAC AATGTGGCCACCTTCTGCATGCTGCTAATGCTCTTCATCTTCATCTTCAGCATCCTGGGCATGCACCTGT TTGGCTGTAAGTTCAGCCTGAAGACAGACTCTGGAGACACCGTCCCTGACAGGAAGAACTTCGACTCTCT ACTGTGGGCCATCGTCACTGTGTTTCAGATCTTGACACAGGAAGACTGGAATGTGGTTCTTTACAACGGC ATGGCTTCCACCTCGTCCTGGGCTGCCCTTTACTTTGTGGCCCTCATGACCTTTGGGAACTATGTGCTCT TCAACCTGCTGGTAGCCATCCTGGTGGAAGGCTTCCAGGCAGAGGGTGATGCCACCAGATCTGACACCGA TGAGGATAAGACATCTACCCACCTAGAGGAAGATTTCGATAAGCTCAGAGACGTTCAAGCCACAGAGATG AAGATGTACTCACTGGCTGTGACCCCTAATGGGCACCTAGAGGGCCGAGGCAGTCTGCCGCCGCCTCTCA TCACGCACACAGCGGCTACGCCTATGCCTACCCCCAAGAGCTCCCCTCATCTGGACATGGCCCACACTCT TTTGGACTCACGGCGCAGCAGCAGTGGCTCTGTGGACCCCCAACTCGGGGACCAGAAGTCTCTGGCTAGC CTGCGCAGCTCCCCTTGTGCCCCATGGGGCCCCAACAGTGCAGGAAGCAGCCGGCGCTCCAGCTGGAACA GCCTGGGCCGCGCACCCAGCCTCAAACGCCGCAGCCAGTGTGGGGAGCGCGAGTCCCTGCTCTCTGGTGA GGGGAAGGGCAGCACGGATGATGAGGCCGAGGACAGCAGACCAAACTCAGGGACCCACCCAGGAGCTTCA CCCGGGCCCCGGGCCACACCCCTGCGGCGGGCTGAGTCGTTGGGCCACCGCAGCACAATGGACCTGTGCC CCCCACGGCCTGCCACCCTCCTGCCCACCAAGTTCCGTGACTGCAACGGGCAGATGGTGGCCCTGCCCAG CGAGTTCTTCCTGCGCATCGACAGCCACAAGGAGGACGCAGCAGAGTTTGATGATGACATAGAAGATAGC TGCTGCTTCCGCCTGCACAAAGTGCTGGAGCCCTATGCACCCCAGTGGTGCAGCAGCCGGGAGTCCTGGG CCCTGTATCTCTTCCCGCCGCAGAACAGGCTACGCGTCTCCTGCCAGAAAGTCATCGCACACAAGATGTT TGACCACGTGGTCCTTGTCTTCATCTTCCTCAACTGTATCACCATTGCCCTGGAGAGGCCAGACATCGAT CCGGGCAGCACTGAACGGGCCTTTCTCAGCGTCTCCAACTACATCTTCACAGCCATCTTCGTGGTGGAGA TGATGGTGAAGGTGGTAGCCCTGGGGCTGCTGTGGGGTGAGCATGCCTACCTGCAGAGCAGCTGGAATGT GCTGGACGGGCTGCTTGTCCTGGTGTCCCTGGTTGACATCATTGTGGCCGTGGCCTCTGCCGGTGGTGCC AAGATCCTAGGCGTCCTGCGCGTGCTGCGCCTGCTGCGGACCCTGAGGCCTCTGAGGGTCATCAGCCGAG CTCCAGGCCTCAAGCTGGTCGTGGAGACTCTGATATCATCGCTCAGGCCCATCGGGAACATCGTCCTCAT CTGCTGCGCCTTCTTCATTATCTTTGGCATCCTCGGGGTGCAGCTTTTCAAGGGCAAATTCTACTACTGC GAGGGTACAGATACCAGGAATATCACCACCAAGGCCGAGTGCCATGCCGCCCACTACCGCTGGGTGAGGC GCAAATACAACTTTGACAACCTGGGTCAGGCGCTGATGTCTCTGTTCGTGCTGTCATCCAAGGATGGCTG GGTAAACATCATGTACGACGGGCTGGACGCCGTGGGCATCGACCAGCAGCCTGTGCAGAACCACAACCCC TGGATGCTGCTCTACTTCATCTCCTTCCTGCTCATCGTCAGCTTCTTCGTGCTCAACATGTTTGTGGGCG TGGTGGTGGAGAACTTCCACAAGTGCCGGCAGCACCAGGAGGCGGAGGAAGCGCGGCGGCGCGAGGAGAA ACGGCTGCGGCGCCTGGAGAGGAGGCGCAGGAAGGCCCAGCGCCGGCCCTACTACGCAGACTATTCACAC ACGCGCCGCTCCATCCATTCGCTGTGCACCAGCCACTACCTGGACCTCTTCATCACCTTCATCATCTGCC TCAATGTCATCACCATGTCCATGGAGCACTACAACCAGCCCAAGTCTCTGGACGAGGCCCTTAAGTACTG CAACTACGTCTTTACCATCGTCTTCGTCTTTGAGGCCGCACTGAAGCTGGTGGCCTTTGGATTCCGGAGG TTCTTCAAGGACAGGTGGAACCAGCTGGACTTGGCCATCGTCCTCCTGTCCATCATGGGCATTGCGCTGG AGGAGATTGAGATGAATGCTGCCCTCCCCATCAACCCCACCATCATCCGCATCATGCGCGTGCTTCGCAT CGCCCGTGTGCTGAAGCTACTGAAGATGGCCACAGGCATGCGCGCCCTGCTGGACACTGTGGTTCAAGCT CTGCCTCAGGTAGGGAACCTTGGTCTTCTTTTCATGCTCCTGTTTTTTATCTATGCTGCCCTGGGAGTGG AGCTGTTTGGGAGGCTAGAATGCAGCGAGGATAATCCCTGTGAGGGCCTGAGCAGACACGCCACCTTCAC CAACTTTGGCATGGCCTTCCTCACGTTGTTCCGAGTGTCCACTGGGGACAACTGGAATGGGATCATGAAG GATACTCTCCGTGAATGTACCCGTGAAGACAAGCACTGCCTCAGCTACCTGCCCGCGCTCTCCCCCGTCT ACTTCGTCACCTTCGTGCTGGTCGCCCAGTTCGTGCTGGTCAACGTGGTGGTGGCCGTGCTCATGAAGCA CCTGGAGGAGAGCAACAAGGAGGCCCGGGAGGATGCGGAGATGGATGCCGAGATCGAGCTGGAGATCGCA CAGGGGTCCACAGCCCAGCCCCCGTCCACAGCACAGGAAAGCCAAGGTACCGAGCCAGACACCCCGAACC TCCTGGTCGTGCGCAAAGTATCTGTGTCCAGGATGCTCTCGCTACCCAATGACAGCTACATGTTCCGGCC GGTGGCTCCTGCGGCTGCCCCGCATTCCCACCCACTGCAGGAAGTGGAGATGGAGACCTACACAGGTCCC ATCAGCTGCGTCCTCCCCAGCCAGGGTCAGCGACCCCCTTTGTGCCCTCTCACCCCGGGATACGCCCCGC TCTCTGAGCCTCTCACGGATACTCTGCAGACAGGAGGCCATGCACGCAGAGTCCCTGGAAGGGCAGATAG ATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001163691
Insert Size	6024 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001163691.1, NP_001157163.1
RefSeq Size	8187 bp
RefSeq ORF	6024 bp
Locus ID	58226
Gene Summary	Voltage-dependent Ca(2+) channels mediate the entry of Ca(2+) ions into excitable cells and are involved in a variety of Ca(2+)-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. The protein encoded by this gene is an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009] Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of a coding exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

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