Scn2a (NM_001099298) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6430408L10; A230052E19Rik; Nav; Nav1.2; Scn; Scn2a1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225118 representing NM_001099298 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAATCAGTGCTGGTACCGCCAGGACCTGACAGCTTCCGCTTCTTTACCAGGGAATCCCTTGCTG CTATTGAACAACGCATTGCAGAAGAGAAAGCTAAGAGACCCAAACAGGAACGCAAGGACGAAGACGACGA AAATGGCCCAAAGCCAAATAGTGACTTGGAAGCAGGAAAATCCCTTCCTTTTATTTATGGAGACATTCCT CCAGAGATGGTGTCAGAGCCCCTGGAGGACCTGGACCCCTACTATATCAATAAGAAAACCTTTATAGTAT TGAATAAAGGGAAAGCAATCTCTCGGTTCAGTGCCACCTCCGCCCTGTACATTTTAACTCCCTTCAACCC CATTAGAAAATTAGCTATTAAGATTTTGGTACATTCTTTATTCAATGTGCTTATCATGTGCACAATTCTT ACCAACTGTGTATTTATGACCATGAGCAACCCTCCAGACTGGACAAAGAATGTGGAGTATACTTTTACAG GAATTTATACTTTTGAATCACTTATTAAAATACTTGCAAGGGGCTTTTGTCTAGAAGATTTCACATTTCT ACGGGATCCCTGGAACTGGCTGGATTTCACAGTCATTACCTTTGCGTATGTAACAGAATTTGTAAACCTA GGCAATGTTTCAGCTCTTCGAACTTTCAGAGTCTTGAGAGCTTTGAAAACTATTTCTGTAATTCCAGGCC TGAAGACCATCGTGGGGGCCCTGATCCAGTCGGTGAAGAAGCTGTCTGACGTCATGATACTCACTGTGTT CTGTCTGAGTGTCTTTGCTCTCATCGGGCTGCAGCTCTTCATGGGCAACCTGAGGAATAAATGCTTGCAG TGGCCCCCAGACAATTCTACCTTTGAAATAAACATCACTTCCTTCTTTAATAACTCATTGGATTGGAATG GTACTGCCTTCAATAGGACAATGAACATGTTTAACTGGGATGAATATATTGAAGATAAAAGTCACTTTTA TTTTTTGGAAGGACAAAACGATGCTCTGCTTTGTGGTAACAGCTCGGATGCTGGCCAGTGTCCGGAAGGA TACATCTGTGTGAAGGCTGGGAGAAACCCCAACTATGGCTACACGAGTTTTGACACCTTCAGCTGGGCCT TCTTGTCCTTATTTCGTCTTATGACTCAAGACTTCTGGGAAAACCTTTATCAGCTGACCTTGCGTGCTGC TGGGAAAACATATATGATATTTTTCGTGCTTGTGATTTTCTTGGGCTCATTCTACCTAATAAATTTGATC CTGGCTGTGGTGGCCATGGCCTATGAAGAACAGAACCAGGCCACGCTGGAGGAAGCTGAACAGAAGGAGG CAGAGTTTCAGCAGATGCTGGAGCAACTTAAGAAGCAGCAAGAAGAAGCTCAGGCAGCAGCTGCAGCAGC GTCTGCAGAATCCAGAGACTTCAGTGGAGCAGGTGGGATAGGTGTTTTCTCGGAGAGTTCTTCCGTAGCT TCAAAGTTAAGTTCCAAGAGTGAAAAGGAGCTGAAAAACAGAAGGAAGAAAAAGAAACAGAAAGAGCAGG CTGGAGAGGAAGAGAAGGAAGATGCTGTGCGGAAATCTGCCTCTGAAGACAGCATACGAAAGAAAGGCTT CCGGTTTTCCCTAGAAGGGAGTAGACTGACCTACGAGAAGAGGTTTTCCTCTCCACACCAGTCTCTCTTG AGCATCCGAGGCTCCCTTTTTTCTCCGAGACGCAACAGTAGAGCAAGCCTTTTCAGCTTCAAAGGTCGGG TGAAGGATATTGGTTCTGAAAATGACTTTGCGGACGATGAACACAGCACGTTTGAGGACAACGACAGCAG GAGAGACTCTCTATTTGTACCACACAGACATGGAGAAAGGCGTCCCAGCAACGTTAGCCAGGCCAGCCGT GCCTCCCGGGGGATACCCACTCTACCCATGAATGGGAAGATGCACAGTGCAGTGGACTGCAACGGTGTGG TCTCCCTAGTTGGAGGCCCTTCTGCTCTCACATCTCCTGTGGGGCAGCTACTACCAGAGGGCACAACTAC TGAAACAGAAATAAGGAAGAGGAGGTCCAGTTCTTACCATGTTTCTATGGACTTGTTGGAAGACCCTACA TCAAGGCAAAGAGCAATGAGTATGGCCAGCATTTTGACCAACACTATGGAAGAACTTGAAGAATCCAGAC AGAAATGCCCTCCATGCTGGTATAAATTTGCTAATATGTGCCTGATTTGGGACTGTTGTAAGCCATGGCT AAAGGTAAAACATGTTGTCAATCTGGTAGTGATGGATCCATTCGTTGACCTGGCCATCACCATCTGCATT GTGTTAAATACACTCTTCATGGCCATGGAGCACTACCCGATGACGGAGCAGTTCAGCAGTGTGCTGTCTG TTGGGAATTTGGTCTTCACTGGGATCTTCACCGCAGAAATGTTTCTCAAGATAATAGCCATGGATCCATA TTATTACTTTCAAGAAGGCTGGAATATCTTTGATGGCTTTATAGTGAGCCTTAGTTTAATGGAACTCGGC TTGGCGAATGTGGAAGGACTGTCAGTTCTCCGATCTTTCCGACTGCTTAGAGTCTTCAAGTTGGCAAAAT CCTGGCCCACACTGAATATGCTCATTAAGATCATCGGCAACTCGGTGGGCGCGCTGGGCAACCTGACCCT GGTGCTGGCCATCATCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTTTGGAAAGAGCTACAAGGAG TGTGTTTGCAAGATTTCCAATGATTGTGAGCTGCCGCGCTGGCACATGCATGACTTCTTCCACTCGTTCC TGATCGTGTTCCGCGTGCTGTGTGGGGAGTGGATAGAGACCATGTGGGACTGCATGGAGGTCGCAGGCCA GACCATGTGTCTTACTGTCTTCATGATGGTCATGGTGATTGGGAACCTTGTCGTTCTGAACCTCTTCTTG GCCTTGCTCCTCAGTTCTTTCAGCTCGGACAACCTGGCTGCCACAGATGACGATAACGAAATGAACAACC TCCAGATAGCCGTGGGAAGGATGCAGAAGGGGATTGATTTTGTTAAAAGGAAGATACGTGAATTCATTCA GAAAGCCTTTGTCAGAAAGCAGAAAGCTCTAGATGAAATCAAACCCCTTGAAGATCTGAATAACAAGAAA GACAGTTGTATCTCCAACCACACAACCATAGAAATAGGCAAGGACCTGAATTACCTCAAAGATGGAAACG GGACGACCAGTGGCATAGGCAGCAGTGTGGAGAAGTACGTGGTAGATGAGAGTGATTACATGTCATTCAT CAACAACCCCAGCCTCACCGTGACTGTGCCCATTGCTGTGGGAGAGTCTGACTTTGAAAACTTAAATACA GAAGAATTCAGCAGTGAATCAGATATGGAAGAAAGCAAGGAGAAATTGAATGCAACTAGTTCATCTGAAG GCAGTACAGTTGATATAGGTGCTCCTGCAGAGGGAGAGCAACCAGAGGCCGAACCAGAAGAGTCCCTTGA GCCAGAAGCCTGTTTCACAGAAGACTGTGTAAGAAAGTTCAAGTGTTGTCAGATAAGCATAGAAGAAGGC AAAGGCAAACTCTGGTGGAACTTGAGGAAAACCTGCTACAAGATAGTAGAGCACAACTGGTTTGAAACCT TCATCGTCTTCATGATTCTGCTCAGCAGCGGTGCTCTGGCCTTTGAAGACATTTACATCGAGCAGCGAAA AACCATCAAGACCATGCTGGAGTATGCTGACAAGGTCTTCACTTACATCTTCATCCTGGAGATGTTGCTA AAGTGGGTTGCATATGGTTTCCAAATGTACTTCACCAATGCCTGGTGCTGGCTGGACTTCCTGATCGTTG ATGTCTCACTGGTTAGCTTAACTGCAAATGCCTTGGGCTATTCAGAACTTGGTGCCATCAAATCCCTCCG AACATTAAGAGCACTGAGGCCTCTACGAGCCTTATCCCGATTTGAAGGAATGAGGGTTGTTGTAAACGCT CTCCTAGGTGCAATCCCATCCATAATGAACGTACTTCTGGTCTGCCTGATCTTTTGGCTAATATTCAGTA TCATGGGGGTGAATCTCTTTGCCGGCAAGTTCTATCATTGCATCAACTACACCACCGGGGAAATGTTTGA TGTGAGCGTGGTCAACAACTACAGTGAGTGCCAGGCTCTCATAGAGAGCAATCAGACAGCCAGGTGGAAG AATGTGAAAGTCAACTTTGACAACGTGGGACTTGGATATCTTTCTCTGCTTCAAGTAGCCACATTTAAAG GATGGATGGATATCATGTATGCAGCTGTTGACTCAAGAAATGTAGAACTGCAACCCAAATATGAAGACAA TCTATACATGTACCTTTACTTTGTCATCTTTATTATTTTCGGCTCATTCTTCACACTGAACCTGTTCATC GGCGTCATCATAGATAACTTCAACCAACAGAAGAAGAAGTTTGGAGGTCAAGACATTTTTATGACAGAAG AACAGAAGAAATACTACAATGCAATGAAGAAGCTAGGCTCAAAGAAACCACAAAAACCGATACCTCGCCC TGCTAACAAGTTTCAAGGGATGGTCTTTGATTTTGTAACCAAACAAGTGTTTGACATCAGCATCATGATC CTCATCTGCCTCAACATGGTGACCATGATGGTGGAAACGGACGACCAGAGCCAAGAGATGACCAACATCC TGTATTGGATCAACCTGGTGTTCATCGTCCTGTTCACCGGCGAGTGTGTGCTCAAGCTTATCTCACTCCG TCATTATTATTTCACCATTGGATGGAACATTTTTGATTTTGTGGTGGTCATCCTCTCCATTGTAGGGATG TTTCTCGCGGAGCTGATAGAGAAGTATTTCGTGTCCCCTACCCTGTTCCGAGTCATCCGCCTGGCCAGGA TTGGACGAATCCTACGCCTGATCAAAGGCGCCAAGGGGATCCGCACGCTGCTCTTTGCTCTGATGATGTC CCTTCCTGCGCTGTTTAACATCGGCCTCCTGCTTTTCCTGGTCATGTTCATCTACGCCATCTTTGGGATG TCCAACTTTGCCTACGTTAAGAGGGAAGTTGGGATTGATGACATGTTCAACTTTGAGACCTTTGGCAACA GCATGATCTGCCTGTTCCAAATCACCACTTCTGCGGGCTGGGATGGACTGCTGGCCCCTATACTAAATAG TGGACCTCCCGATTGTGACCCTGAAAAAGATCATCCTGGAAGCTCGGTGAAGGGAGACTGTGGGAACCCA TCTGTGGGGATTTTCTTTTTTGTCAGCTACATCATCATATCCTTCCTGGTTGTGGTGAACATGTACATTG CTGTCATCCTGGAGAACTTCAGCGTTGCCACAGAAGAAAGTGCAGAGCCTCTGAGTGAGGACGACTTTGA GATGTTCTACGAAGTCTGGGAGAAGTTCGACCCTGACGCCACCCAGTTCATAGAGTTCTGCAAGCTCTCT GACTTTGCAGCTGCCCTGGATCCTCCCCTCCTCATCGCAAAGCCAAACAAAGTGCAGCTCATTGCCATGG ACCTGCCCATGGTGAGTGGAGACCGCATCCACTGCCTGGACATCTTATTTGCTTTTACAAAGCGGGTCCT GGGTGAGAGTGGAGAGATGGATGCTCTTCGAATCCAGATGGAAGAGCGGTTCATGGCTTCCAATCCCTCC AAGGTCTCTTATGAGCCCATTACCACCACTCTGAAGCGCAAACAAGAGGAGGTGTCTGCTATTGTCATCC AGCGAGCTTACAGACGCTATCTTCTGAAACAGAAAGTTAAGAAGGTTTCGTCTATATATAAAAAAGACAA GGGTAAAGAAGATGAGGGAACGCCCATCAAAGAAGACATCATCACGGATAAACTGAATGAGAATTCAACT CCAGAGAAGACTGACGTGACACCCTCCACCACTTCGCCACCTTCCTACGATAGCGTGACCAAACCGGAGA AGGAAAAATTTGAAAAGGACAAATCAGAAAAAGAGGACAAGGGGAAAGATATCAGGGAAAGTAAAAAGTA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001099298
Insert Size	6021 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001099298.2, NP_001092768.1
RefSeq Size	8690 bp
RefSeq ORF	6021 bp
Locus ID	110876
UniProt ID	B1AWN6
Gene Summary	Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with four repeat domains, each of which is composed of six membrane-spanning segments, and one or more regulatory beta subunits. Voltage-gated sodium channels are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. In humans, variants of this gene are associated with seizure disorders and autism spectrum disorder. Mice homozygous for a knockout mutation die with severe hypoxia and extensive neuronal cell death, while gain of function mutations result in progressive seizure disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.