Myof (NM_001099634) Mouse Untagged Clone

CAT#: MC225147

Myof (untagged) - Mouse myoferlin (Myof), (10ug)



  "NM_001099634" in other vectors (2)

CNY 32,780.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2310004N10Rik; 2310051D19Rik; E030042N20Rik; Fer1; Fer1l3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225147 representing NM_001099634
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCGAGTGATTGTGGAATCTGCCACTAACATCCCTAAAACAAAATTTGGGAAGCCGGATCCTATCG
TTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAACTCAACCCTGTCTGGAA
TGAGATTTTGGAATTTGACTTGAGGGGGATACCACTGGACTCTTCATCCTCCCTGGTGATTGTTGTCAAA
GATTTTGAGACAATTGGACAAAATAAATTAATTGGCACAGCAACTGTCTCCCTGAAGGACCTGATTGGGG
ACCAGAACAGGTCTTTGCCCTACAAACAGACCTCCCTGCTAAATGAAAAGGGACAAGACACTGGGGCCAC
CATTGACCTGGTGATCGGCTATACCCCACCTTCAGCTCCACATCCAAATGATCCGAGCGGGACCAGTGTG
CCAGGCATGGGAGAAGAAGAGGAAGAAGATCAAGGAGATGAAGACAGGGTAGATGGCATAGTCAGAGGCC
CTGGGCCCAAGGGGCCATCTGGGACAGTGTCAGAAGCTCAGCTGGCTCGGAGGATCACCAAAGGGAAGAG
CAGCCGAAGGATGCTGTCCAACAAGCCACAGGATTTCCAGATCCGTGTCAGAGTGATCGAGGGCCGTCAG
TTATGCGGCAACAACATAAGGCCCGTGGTGAAAGTTCACATCTGTGGTCAGACGCACCGCACGAGAATCA
AGAGAGGGAACAATCCGTTTTTTGACGAGTTATTTTTCTACAATGTGCACATCACACCCTCTGAGCTCAT
GGATGAGATCATCAGTATCCGGGTTTATAATTCCCATTCTTTGAGGGCAGATTGTCTGATGGGGGAATTT
AAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCACGCTGTCATGAGGAAATGGCTCCTTCTCAATG
ACCCAGAAGACACCAGCTCAGGCGCAAAAGGTTACATGAAAGTCAGCATGTTTGTCCTGGGAACCGGAGA
CGAACCCCCTCCTGAGAAACGAGACCGTGACAATGACAGTGATGACGTGGAGAGCAACTTGTTACTTCCG
GCAGGCATTGCCCTCCGGTGGGTGACCTTCATGCTGAAGATCTACCGAGCCGAGGACATCCCCCAGATGG
ATGATGCCTTCTCACAAACAGTGAAGGAAATATTTGGTGGCAACGCAGATAAAAAAAATCTTGTGGATCC
TTTTGTAGAAGTCTCCTTTGCTGGGAAAAAGGTGTGCACAAATATAATTGAGCGGAATGCAAACCCTGAA
TGGAATCAAGTTGTCAACCTTCAGATCAAGTTTCCCTCCATGTGTGAAAAAATAAAACTAACAGTATATG
ACTGGGACCGGCTTACTAAAAATGATGTAGTTGGGACCACGTACCTGTACCTCTCTAAAATTGCTGCCTC
TGGGGGAGAAGTGGAAGCAACTACAGGAGAGACAGAGGTGGGGTTTGTCCCAACATTCGGACCTTGTTAT
TTGAACCTTTACGGAAGCCCCAGAGAGTACACAGGATTTCCAGATCCCTATGATGAGCTCAACTCAGGAA
AGGGAGAAGGGGTGGCCTACAGAGGCAGGATCTTCGTGGAACTGAACACGTTCCTTGAAAAGAAACCACC
AGAAAAGAAGCTGGAGCCCATTTCCAGTGATGACCTTCTGGTTGTTGAGAAGTACCAGCGAAGGCGGAAG
TATAGCCTGTCCGCCGTGTTCCATTCAGCCACCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAG
TCAGCATTGGGAACTATGGCAACAAGTTTGATGCAACCTGTAAACCTTTGGCATCAACCACTCAATACAG
CCGTGCAGTATTTGACGGGAACTACTATTACTACTTACCTTGGGCCCACACGAAGCCCGTGGTCACCCTG
ACTTCCTACTGGGAGGATATTAGTCACCGCCTGGATGCAGTGAACACTCTCCTAGTCATGGCAGAACGGC
TGCAATCAAACATAGAGGCTGTAAAATCAGGGATCCAGGGTAAAATTCCTGCAAACCAGTTGGCGGAGGT
GTGGCTGAAGCTGATAGATGAAGTTATAGAAGACACGAGATACACCTTGCCTGTCACAGAAGGAAAAGCC
AACGTCACGGTTCTGGATACTCAGATCCGAAAGTTGCGGTCCAGGTTTCTCTCCCAGATACACGAGGCAG
CTCTGAGGATGAGATCTGAAGCCACGGATGTGAAATCCACCTTGTTGGAAATCGAGGAGTGGCTTGATAA
ACTGATGCAGCTTACTGAAGAGCCTCAGAACAGCATGCCTGACATCATCATCTGGATGATCCGTGGAGAG
AAAAGACTGGCTTATGCACGAATCCCTGCCCACCAAGTCTTGTACTCCACCAGCGGTGGGAACGCCTCTG
GAAAATACTGTGGGAAAACCCAAACCATCCTTCTGAAGTATCCACAAGAAAAAACCAATGGGCCCAAAGT
GCCTGTGGAGCTGCGCGTGAACATCTGGCTGGGTTTAAGTGCTGTGGAAAAGAAGTTCAATAGCTTCGCG
GAAGGCACCTTCACCGTCTTTGCTGAAATGTATGAAAACCAAGCCCTTGTGTTTGGGAAGTGGGGCACCT
CTGGACTGGTGGGACGCCATAAATTCTCTGATGTCACAGGAAAAATAAAACTCAAGAGAGAATTCTTTTT
ACCTCCCAAAGGCTGGGAGTGGGAAGGAGACTGGGTGGTTGACCCTGAACGAAGCCTCCTGACTGAGGCT
GACGCAGGTCACACCGAGTTCACTGATGAAGTCTACCAGAATGAGAATCGCTACCCCGGAGGCGAGTGGA
AGCAGGCAGAGGACACCTATACGGATGCGAATGGAGATAAGGCGGCATCACCCAGTGAGATGACCTGCCC
TCCAGGTTGGGAGTGGGAGGATGACGCATGGATATATGACATAAATCGAGCAGTGGATGAGAAAGGCTGG
GAGTATGGGATTACCATTCCTCCTGATAATAAGCCCAAATCCTGGGTTGCAGCAGAAAAGATGTATCACA
CTCACAGGCGGCGGAGACTGGTCCGGAAACGCAAGAAAGATCTGACACAGACGGCTTCTAGTACCGCAAG
GGCCATGGAAGAACTTGAAGACCGGGAGGGCTGGGAATATGCTTCGTTAATCGGCTGGAAATTCCACTGG
AAACAGCGCAGTTCAGACACCTTTCGCCGCAGGCGCTGGAGGAGGAAAATGGCCCCTTCTGAGACACACG
GTGCCGCTGCCATCTTTAAACTCGAAGGTGCCCTTGGGGCAGACACCACAGAGGATGGAGAAGAGAAGGG
CCCGGAGAAGCAGAAGCACAGCGCCACCACTGTGTTTGGAGCAAACACGCCCATTGTTTCCTGTAATTTC
GACAGAGTCTACATCTACCACCTGCGCTGCTACATTTATCAAGCCAGGAACCTCATGGCTTTGGACAAAG
ACAGCTTTTCAGATCCATACGCCCATGTCTCCTTCCTCCATCGAAGCAAAACCACTGAGATCATCCACTC
CACCCTGAATCCCACCTGGGACCAAACCATTATATTCGATGAGGTTGAAATCTTTGGGGAACCCCAAACA
GTTCTACAGAACCCACCCAACGTTACCATAGAACTTTTTGACAATGACCAAGTGGGCAAAGATGAATTTT
TAGGTCGAAGCATTTGCTCTCCCTTGGTGAAACTGAACTCAGAAACGGACATCACACCTAAGCTTCTCTG
GCATCCTGTTATGAATGGAGACAAGGCCTGTGGGGATGTCCTCGTAACAGCAGAGTTGATTCTGAGGAAC
AAGGATGGCTCCAACCTTCCCATCCTCCCCTCTCAAAGAGCACCAAACTTATACATGGTCCCTCAGGGGA
TCAGGCCTGTGGTTCAGCTCACTGCCATTGAGATCCTAGCTTGGGGCTTAAGAAATATGAAAAACTACCA
GATGGCTTCTGTCACATCACCCAGCCTGGTGGTGGAATGCGGAGGAGAGAGAGTAGAATCGGTGGTGATC
AAAAGCCTTAAGAAGACACCCAACTTCCCAAGTTCTGTTCTCTTCATGAAAGTGTTCTTGCCCAAGGAAG
AATTGTACATGCCTCCGCTGGTGATCAAGGTCATTGATCACAGGCAGTTTGGGCGGAAGCCTGTGGTTGG
CCAATGCACCATTGACCACCTGGACCGCTTTCGGTGTGATCCATATGCAGGAAAGGAGGATATTGTACCA
CAACTAAAAGCCTCTCTGATGTCTGCACCTCCATGCCGGGAAGTCGTTATTGAAATAGAAGACACCAAAC
CATTACTGGCTTCTAAGCTGTCGGAAAAGGAGGAAGAAATCGTTGACTGGTGGAGTAAATTTTATGCTTC
CTCTGGAGAGCATGAAAAATGTGGACAGTATATTCAGAAAGGCTATTCCAAACTCAAGATCTATGACTGT
GAACTGGAGGATGTAGCCGACTTTGAAGGCCTGACAGATTTCTCAGATACCTTCAAGCTGTACAGAGGCA
AGTCCGATGAGAATGAAGATCCATCTGTGGTAGGAGAGTTTAAAGGCTCCTTCCGAATCTACCCCTTGCC
GGATGACCCCAGTGTGCCAGCCCCTCCCAGGCAGTTTCGGGAACTGCCTGACAGTGTCCCACAGGAATGC
ACTGTTAGGATTTACATTGTTCAAGGCTTACAGCTCCAGCCTCAGGACAACAATGGCCTGTGTGATCCTT
ACATAAAAATAACCTTGGGCAAGAAAGTAATTGAAGACCGAGACCACTACATTCCCAACACTCTCAACCC
AGTGTTTGGCAGAATGTACGAACTGAGCTGCTACTTGCCTCAAGAGAAAGACCTGAAGATCTCTGTCTAT
GACTACGATACCTTCACCCGAGATGAGAAGGTGGGAGAGACAACCATTGACCTGGAGAACCGCTTCCTTT
CCCGGTTCGGGTCTCACTGCGGAATCCCTGAGCAGTACTGTGTTTCCGGAGTAAATACCTGGCGAGATCA
GCTGAGACCAACGCAGTTGCTTCAAAACGTTGCAAGATTCAAAGGCTTCCCGCCACCAGTTCTTTCTGAA
GACGGAAGCAGAATCCGATACGGAGGTCGAGACTACCATTTGGATGAGTTTGAGGCCAACAAAATCCTGC
ACCAGCACCTTGGAGCCCCCGAAGAGCGTCTTGCCCTCCATATTCTCAGGACTCAGGGGCTGGTCCCTGA
GCACGTGGAGACAAGGACTTTGCACAGCACCTTCCAGCCCAATATTTCCCAGGGGAAACTTCAGATGTGG
GTGGATGTCTTCCCCAAGAGTTTAGGACCACCAGGCCCCCCTTTCAACATCACACCCAGGAAAGCCAAGA
AGTACTACCTGCGTGTGATCATCTGGAATACCAAGGACGTTATCTTGGATGAGAAGAGCATCACTGGGGA
GGACATGAGTGACATCTACGTCAAAGGCTGGATTTCCGGCAGCGAAGAAAACAAACAGAAAACAGATGTC
CACTACAGGTCCTTGGATGGTGAAGGCAATTTTAACTGGAGATTTGTTTTCCCATTTGACTACCTCCCTG
CTGAGCAGCTCTGTATAGTCGCTAAAAAAGAGCATTTCTGGAGTATTGATCAAACAGAATTTCGAGTCCC
TCCTCGGCTGATCATTCAGATATGGGACAATGACAAGTTTTCCCTGGACGACTACTTGGGTTTTCTGGAA
CTTGACTTACATCGTACGATCATCCCAGCCAAGACATCAGAGAAATGCAGTTTGGACATGATTCCAGACC
TCAAAGCCATGGACCCTCTTAAAGCTAAGACTGCCTCACTCTTTGAACAGAGGTCCATGAAAGGATGGTG
GCCGTGCTACGCAGACAAAGATGGCACCCGGGTGATGGCTGGGAAAGTGGAGATGACACTGGAGGTGCTC
AACGAGAGGGAGGCCGACGAGAGGCCAGCCGGGAAGGGGCGGAGTGAACCCAACATGAACCCCAAGCTTG
ACCCACCAAACCGACCGGAAACCTCCTTCCTCTGGTTCACGAACCCTTGCAAGACCATGCGCTTCATCGT
GTGGCGCCGGTTCAAGTGGGTGATCATCGGCCTGCTTCTGCTGCTCATCCTGTTGCTGTTCGTGGCTGTG
CTCCTGTACTCTCTTCCGAACTATTTATCAATGAAGATTGTGAGGCCGAATGCATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001099634
Insert Size 6147 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001099634.1, NP_001093104.1
RefSeq Size 7094 bp
RefSeq ORF 6147 bp
Locus ID 226101
UniProt ID Q69ZN7
Gene Summary The protein encoded by this gene is a member of the ferlin family of proteins, which have been implicated in fusion events in muscle tissue. Members of this family have a carboxy-terminal single pass transmembrane domain and multiple C2 domains, which bind negatively charged phospholipids in the presence of calcium ions. This gene is expressed at high levels in myoblasts and upregulated in damaged skeletal muscle. Mice deficient in this protein display defects in myoblast fusion, muscle regeneration, and angiogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Transcript Variant: This variant (2) lacks an in-frame exon in the coding region compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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