Ralgapa1 (NM_001003719) Mouse Untagged Clone

CAT#: MC225162

Ralgapa1 (untagged) - Mouse Ral GTPase activating protein, alpha subunit 1 (Ralgapa1), transcript variant 3, (10ug)



  "NM_001003719" in other vectors (2)

CNY 33,440.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2310003F20Rik; 4930400K19Rik; AI563624; Garnl1; GRIPE; mKIAA0884; Tulip1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225162 representing NM_001003719
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG
CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGACCTTAAACAGTTTTT
TGACCAACATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA
CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC
TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA
CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG
GATAACTGCAGCAAAGAACAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTCTCAGCACCACAATCCG
AATATGGACCTCGGACTTTAGATAATCTTATTAATCCTCCACTCAGCCTTCAAGAAACTCAAGTAACTAT
AGAGGAAGTCACTCCTCTTGTTCCGCCACAGTCAGGAGATAAAGGACAAGAAGACCTCACAAGCTATTTT
CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG
AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACATTTG
CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGATCAGACCAAAGCCTCATTATGTGATG
ATAAAGAAGGATGCTGAAACCAATGAAACAATATATTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTTA
TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATTCAGGACCTAATATTCCTGGGAT
GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTTTCCAGAGAAGAAAGCAAA
AATGATACTGTTGATAAAGTAGACAAGTCTGCAGAGCCTGAGCAGTCTCATTCCAACACAAGCACTCTCA
CGGAGCGAGAGCCTAGCTCTTCCAGTCTCTGCAGCATCGACGAAGAACATCTCACAGACATCGAAATTGT
TCGCAGAGTGTTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA
TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTGAAAGTGTATCAAGAATGGATTCAACAAGAGG
AAAAGCCTTTGTTTATGCAAGAGCCTGAGGACACTGCGATCACTTGTTCAGATATACCCTGCAGCGAAAC
TGTTGCAGACCATGACAGTGCAATAGAAGATGGAGAGAAAAGAGAAGAGGAAAATGGGACCAGTACTTCT
GAACATGTTCGCAATTCTAGCTGGACAAAAAATGGTTCCTACCAAGAAGCTTTTCATGTCTGTGAAGAAG
CCACAGAACAGAATATTCAAGCTGGCACTCAGGCCGTTTTGCAGGTGTTTATTATAAACTCATCGAACAT
ATTTCTTCTTGAACCTGCAAATGAAATAAAGAATCTTCTGGATGAGCATACAGATATGTGTAAACGCATT
CTTAATATTTATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTGCTCG
TGCTGCTCAGAGTCACGGAGTCCGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA
AAGCATGACCCTGGCGGGTCGACTTGCAGGACCACTTTTTCAGACTCTCATTGTTGCCTGGATCAAAGCA
AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCGGTATTGTCATCATTGACCTATT
GGGAAGAATTGGCCACTGAGTGGTCACTGACCATGGAGACACTGACTAAAGTTTTGGCTAGAAATTTATA
TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA
GGAGTTGGACACGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC
CTGGCCAGGCACCTATGAGACAGAGAAGTGCAACGACCACTGGCTCCCCAGGAACTGAGAAGGCCAGGAG
TATAGTACGGCAAAAAACTGTTGATATTGATGATGCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACAT
TTTTCACAGAGTGAAGACACTGGAAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGAA
GTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGAG
CCCCAAACTGCCTCCTCTTAATAGTGAGACTGGTGGCAACAGCGCCAATGTTCCTGACCTGATGGATGAG
TTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGGAGTAGTCCAGGCAGCC
TTGAAATCCCGAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGGCCTGTTGATGACCCTGG
TGTACCCTCCGAATGGACGTCTCCAGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTCG
GATTCCTTCAGTGCCTTCCAGTGTGAGGGACGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGGA
TCCCATCTTCTGCTGATGTGGATTTAGGTTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCCAG
CCTAACTACGCTTCATTTAGATTCAGAAACAAGCAGCCTTAATCAGCAAGCCTTCTCTGCAGAAGTTGCA
ACCGTTACTGGTTCAGAAAGTGCTTCCCCCGTTCATTCAGCTCTGGGATCCAGGTCGCAGACCCCCTCCC
CATCTACACTGAGCAGAGCTCACATAGAACAGAAGGACCTACAGCTCGATGAGAAGCTCCACCACTCTGT
TCTTCAGACGCCGGATGACCTAGAAATCAGTGAATTTCCATCTGAATGTTGTAGCGTGATGGCAGGAGGT
ACTCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGCAGAATGCTGGGCATTTTGGGAGATG
TCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCT
AGCCAAGATCAGAGATAACCTTGGCATTTCAGCTGACAACCTGACTTCCCCTTCTCCACCCGTCCTGATT
CCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGTA
AATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCTCCAAATAGAGATTT
TTTAACACATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATTGTCAATACGATC
ATCAAGCACTGCTCACCTCAGTTTTTTTCACTCGGTTTGCCTGGGGCCACGATGCTTATTATGGATTTTA
TTATAGCAGCTGGGAGAGTGGCTTCTTCGGCTTTTCTTAATGCACCAAGAGTGGAAGCACAAGTTCTTCT
GGGATCTTTAGTTTGCTTTCCCAACTTATATTGTGAATTGCCTGCTCTCCATCCCAACATTCCTGATATT
GCTGTGTCTCAATTTACAGATGTTAAGGAACTTATAATTAAAACTGTATTAAGCTCGGCAAGAGATGAGC
CCTCTGGTCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATTTGGATTTGTGAAGAATTAGTCCACGA
ATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAACA
GTTGCCCACGTCGCCTGTAACATGCTTCACATGCTGGTTCACTATGTCCCTAGACTTCAGATTCACCAGC
CGCAGTCTCCCCTGAAAATTATTCAGATCCTAATAGCAACTATCACCCACCTTTTGCCAAGTACAGAAGC
TTCATCATATGAAATGGACAAGAGGTTGGTTGTCTCATTACTTCTTTGCCTTCTGGACTGGATCATGGCC
TTACCTTTAAAGACACTGCTCCAACCAGTCCATGCTACAGGAGCAGAAAATGATAAGACCGAAAAGTCTG
TTCTCAACTGCATTTATAAGGTATTACATGGGTGTGTCTATGGAGCGCAAAGTTTTAGCAATCCCAAGTA
CTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGATCCGTTTATGCATTTGGAAAGTCTGAAA
GAACCTGAACCTTTACACTCTCCAGATTCAGAGCGGTCTTCTAAACTGCAGCCTGTGACAGAAGTGAAAA
CTCAGATGCAACAAGGATTAATCTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACTTGGG
CCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCATGACAATCATTAC
AGTGAAAGTACTGAACTTTCTCCTGAGCTCTTTGAAAGTCCAAACATCCAGTTCTTTGTGCTGAATAATA
CAACCTTAGTGTCCTGTATCCAGATAAGATCGGAAGAGAGTATGCCTGGAGGAGGTCTAGCTGCTGGCCT
TGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGCC
ATACTGTATGGACCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTGACC
AAGAGAAACCGGAAGAGCCTCCTACATCTAACGAATGCTTAGAAGACATCGCAGTAAAAGATGGGCTTTC
TCTCCAGCTTAGAAGATTTAGAGAAACTGTACCAACTTGGTCTACGATACGAGAGGAAGAAGATGTTCTT
GACGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAGTGTTTACAGAGAACGGGAATCTCCCTCAATG
TTCCTGCTCCACAGCCTCTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGCA
ATACACGGAAGAAAAAGAGTTTGTAGAGAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGAT
GAGCCAACACCTCAGAAACCTCAGTCAGCTTTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATGA
ATTCCTGGGACAAACGGAGGAGCTTTCATCTCTTGAAGAAAAATGAAAAGCTGCTCAGAGAACTTAGGAA
CTTGGATTCAAGGCAATGCCGAGAGACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGAT
AAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTAGCTGGCCTTGGTTGGG
AGGTAAACCTTACAAACCACTGTGGCTTCATGGGAGGCCTTCAGAAGAACAGAAGCACTGGACTGACCAC
TCCCTATTTTGCTACCTCGACAGTAGAAGTAATATTTCATGTATCTACACGAATGCCTTCTGATTCTGAT
GACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTGCACATTGTTTGGTCAGAACATACTA
GAGACTACAGGAGAGGAATTATTCCTACTGAGTTTGGTGATGTCCTCATTGTAATATACCCAATGAAAAA
CCACATGTTCAGTATCCAGATCATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCACTTTTTGATGGTGCT
ATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAAT
CACTGATTCCATTGTATCAAAATTTCTATGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACCA
TTTAGAGCCAACAACATTTGAAGACTTTGCAGCACAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCCT
GCTGACGCAGTTGGATCCTACCCAGAGATTCCACCCAGTGACGCTGCCCCAGCAGCACAGGTAGACGGGG
CTGACCTGGCCTCTCCCATGTCTCCTCGAACTAGCAAAAGCCGCATGTCCATGAAGCTGCGTCGGTCCTC
TGGCTCAGCCAATAAATCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001003719
Insert Size 6252 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001003719.2, NP_001003719.1
RefSeq Size 8249 bp
RefSeq ORF 6252 bp
Locus ID 56784
UniProt ID Q6GYP7
Gene Summary Catalytic subunit of the heterodimeric RalGAP1 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB (By similarity). May interact with the HLH region of TCF3/isoform E12.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region and an internal exon in the 3' region which results in an alternate downstream termination codon, compared to variant 4. The resulting isoform (3) lacks an internal segment but has a longer and distinct C-terminus, compared to isoform 4.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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