Sptbn1 (NM_009260) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9930031C03Rik; AL033301; elf1; elf3; mKIAA4049; Spnb-2; Spnb2; SPTB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225200 representing NM_009260 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGCAGAGGACATCCAGCATTTCAGGGCCGCTGTCGCCGGCCTACACCGGGCAGGTGCCTTACA ACTACAACCAACTGGAAGGAAGATTCAAACAGCTCCAAGATGAGCGTGAAGCTGTACAGAAGAAGACCTT CACCAAGTGGGTCAATTCCCACCTTGCGAGAGTGTCCTGCCGAATCACAGACCTGTACACGGACCTTCGA GATGGACGGATGCTCATCAAGCTACTGGAGGTCCTCTCTGGAGAGAGGCTGCCTAAACCCACTAAGGGAC GGATGCGGATCCACTGTCTGGAGAATGTCGACAAGGCTCTTCAATTCCTGAAAGAGCAGAGAGTCCATCT TGAGAACATGGGCTCCCATGACATTGTGGATGGAAACCACCGGCTGACCCTCGGCCTCATCTGGACAATT ATTCTGCGCTTCCAGATCCAGGATATTAGTGTGGAGACTGAAGATAACAAAGAGAAAAAGTCTGCTAAGG ATGCATTGCTGCTGTGGTGCCAGATGAAGACAGCTGGGTACCCCAATGTCAACATTCACAATTTCACCAC TAGCTGGAGGGATGGCATGGCCTTCAATGCACTGATACATAAACATCGGCCTGACCTGATAGATTTTGAT AAACTGAAGAAATCTAATGCACACTACAATCTGCAGAATGCATTTAACCTGGCAGAGCAGCACCTTGGCC TCACTAAACTGTTAGACCCTGAAGATATCAGTGTGGACCACCCTGATGAGAAGTCTATCATCACATACGT GGTGACTTACTACCACTACTTCTCCAAGATGAAGGCCTTGGCTGTCGAAGGAAAGCGCATTGGAAAGGTG CTTGATAATGCTATAGAAACAGAGAAAATGATTGAGAAGTACGAGTCACTTGCTTCTGACCTTCTGGAGT GGATTGAACAAACCATCATCATCCTAAACAACCGCAAATTTGCTAATTCACTGGTTGGGGTCCAGCAGCA GCTCCAGGCATTCAACACGTACCGCACAGTGGAGAAACCACCTAAGTTTACTGAGAAGGGGAATTTGGAG GTGCTCCTTTTCACAATTCAGAGCAAGATGCGAGCGAATAATCAGAAGGTCTACATGCCCCGCGAGGGGA AGCTCATCTCTGACATCAACAAGGCCTGGGAAAGACTGGAAAAAGCAGAACATGAGAGAGAACTGGCTCT GCGGAATGAGCTCATACGGCAGGAAAAACTGGAACAACTCGCCCGAAGATTTGATCGCAAGGCAGCTATG AGGGAGACATGGCTGAGTGAAAACCAGCGTCTTGTGTCTCAGGACAACTTTGGATTTGACCTTCCCGCTG TTGAGGCTGCTACCAAAAAACACGAGGCCATTGAGACAGACATCGCTGCATATGAAGAACGAGTTCAGGC CGTGGTGGCTGTGGCCAGGGAACTTGAAGCCGAGAACTACCATGACATCAAGCGCATCACAGCGAGGAAG GACAATGTCATCCGGCTCTGGGAATACTTGCTGGAACTGCTCAGGGCCAGGAGGCAGCGTCTTGAGATGA ACCTGGGATTGCAAAAGATATTCCAGGAAATGCTTTATATTATGGACTGGATGGATGAAATGAAGGTGCT ATTGCTGTCTCAAGACTATGGCAAACACTTACTTGGTGTTGAAGACCTGTTACAGAAGCATGCCCTGGTT GAAGCAGACATTGCAATCCAAGCAGAGCGTGTAAGAGGTGTGAATGCCTCTGCCCAGAAGTTTGCAACAG ATGGGGAAGGCTACAAGCCATGTGACCCCCAGGTAATTCGAGACCGTGTTGCCCACATGGAGTTCTGCTA TCAAGAGCTTTGTCAGCTGGCTGCCGAGCGTAGGGCTCGCCTGGAAGAGTCCCGTCGCCTCTGGAAGTTC TTCTGGGAGATGGCAGAAGAGGAAGGCTGGATACGAGAGAAGGAGAAGATCCTGTCCTCTGATGATTACG GGAAAGACTTGACCAGTGTCATGCGCCTGCTGAGCAAGCACCGGGCATTTGAGGATGAGATGAGTGGCCG TAGTGGCCATTTTGAGCAGGCCATTAAAGAAGGTGAAGACATGATTGCAGAGGAACACTTTGGATCGGAA AAGATCCGTGAGAGAATCATTTATATCCGGGAGCAGTGGGCCAACCTGGAACAGCTCTCAGCCATTAGGA AGAAGCGCCTAGAGGAAGCCTCATTACTGCACCAGTTCCAGGCTGATGCTGATGATATTGATGCTTGGAT GTTAGATATACTCAAGATTGTCTCCAGCAATGATGTGGGCCATGATGAGTACTCCACGCAGTCTCTGGTC AAGAAGCATAAAGATGTAGCAGAAGAGATCACCAACTACAGGCCCACTATTGACACACTGCATGAGCAAG CCAGTGCCCTTCCACAAGCACATGCAGAGTCTCCAGATGTGAAGGGCCGGCTGGCAGGAATTGAGGAGCG CTGCAAGGAGATGGCAGAGTTAACACGGCTAAGGAAGCAGGCTCTGCAGGACACCCTGGCCCTGTACAAG ATGTTCAGTGAGGCTGATGCCTGTGAGCTCTGGATTGACGAGAAGGAGCAGTGGCTCAACAACATGCAGA TCCCAGAGAAGCTGGAGGACCTGGAAGTCATCCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAA CCAGGCTTCCCGGGTTGCTGTGGTGAACCAGATTGCACGGCAGCTGATGCACAATGGCCACCCCAGTGAA AAGGAAATCAGAGCTCAGCAAGACAAACTCAACACGAGGTGGAGTCAGTTCAGAGAACTGGTGGACAGGA AAAAGGATGCTCTTCTGTCTGCCCTGAGCATCCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTG GATCCGGGAGAAGACCAAGGTCATCGAGTCTACCCAAGACCTTGGCAATGACCTGGCAGGTGTCATGGCC CTGCAGCGCAAGCTGACTGGCATGGAACGAGACTTGGTAGCCATTGAGGCGAAGCTGAGTGACCTGCAGA AAGAAGCTGAGAAGCTGGAGTCCGAGCACCCTGACCAGGCTCAAGCTATCCTGTCTCGGCTGGCCGAGAT CAGTGATGTGTGGGAGGAAATGAAGACAACCCTGAAGAACCGAGAGGCCTCCCTGGGAGAGGCCAGCAAG CTGCAGCAGTTTCTGCGGGACTTGGACGACTTCCAGTCTTGGCTCTCCAGGACCCAGACTGCTATCGCCT CAGAGGACATGCCCAATACCCTCACTGAGGCAGAGAAGCTTCTCACACAGCACGAGAATATCAAAAATGA GATCGACAATTATGAGGAAGACTACCAGAAGATGCGGGACATGGGCGAGATGGTCACCCAGGGGCAGACT GATGCCCAGTATATGTTTCTGCGGCAGCGGCTGCAGGCCTTAGACACTGGCTGGAATGAGCTCCACAAAA TGTGGGAGAACAGGCAAAACCTCCTCTCCCAGTCCCATGCCTACCAGCAGTTCCTTAGGGACACCAAACA AGCTGAAGCTTTTCTTAATAACCAGGAGTATGTTTTGGCTCATACTGAAATGCCCACCACCCTGGAAGGA GCTGAAGCAGCCATTAAAAAGCAGGAGGACTTCATGACCACCATGGATGCCAACGAGGAGAAGATCAATG CTGTTGTGGAGACTGGCCGAAGACTGGTGAGCGATGGGAACATCAACTCCGACCGCATCCAGGAGAAGGT GGACTCTATTGACGACAGACACAGGAAGAATCGAGAAGCAGCCAGTGAACTTCTGATGAGGTTAAAGGAC AACCGTGATCTACAGAAGTTCCTGCAAGATTGTCAAGAGCTGTCCCTCTGGATCAATGAAAAGATGCTTA CAGCTCAAGACATGTCTTATGATGAAGCCAGAAATCTGCACAGTAAATGGTTAAAGCATCAAGCATTTAT GGCGGAACTTGCATCCAACAAAGAATGGCTTGACAAAATTGAGAAGGAAGGAATGCAGCTTATTTCAGAA AAGCCAGAAACAGAAGCTGTGGTAAAGGAAAAACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAAT CCACAACCCAGACCAAGGCCCAGCGGCTCTTTGATGCAAATAAGGCTGAGCTTTTCACACAAAGCTGCGC AGATCTTGACAAATGGCTACATGGCCTGGAGAGCCAGATTCAATCTGACGACTATGGCAAAGACCTTACC AGTGTCAATATTCTTCTGAAAAAGCAACAGATGCTGGAGAATCAGATGGAAGTTCGGAAGAAAGAGATCG AGGAACTGCAGAGCCAAGCCCAGGCGCTGAGTCAGGAGGGGAAGAGCACAGATGAGGTGGACAGCAAACG CCTTACTGTGCAGACCAAGTTCATGGAGCTTCTGGAGCCCTTGAGTGAGAGGAAGCATAACCTGTTAGCT TCCAAGGAGATCCATCAGTTCAACAGGGATGTGGAGGACGAAATCCTATGGGTTGGCGAGAGGATGCCTT TGGCAACTTCCACAGATCATGGCCATAACCTTCAAACTGTGCAGCTGTTAATAAAGAAAAACCAGACCCT CCAGAAAGAAATCCAGGGACACCAGCCTCGTATTGATGACATCTTTGAGAGGAGTCAAAACATCATCACA GATAGCAGCAGCCTCAATGCCGAGGCTATCAGGCAGAGGCTCGCTGACCTGAAGCAGCTGTGGGGGCTCC TCATTGAGGAAACTGAGAAACGCCATAGACGGCTGGAGGAGGCACACAAGGCGCAGCAGTACTACTTTGA TGCAGCTGAAGCCGAGGCATGGATGAGTGAACAGGAGTTGTACATGATGTCTGAGGAAAAGGCCAAGGAT GAGCAGAGTGCTGTCTCTATGTTGAAAAAGCACCAGATTTTAGAGCAAGCTGTTGAGGACTATGCAGAGA CAGTACACCAGCTCTCCAAGACTAGCCGGGCGCTGGTGGCTGACAGCCATCCCGAAAGTGAGCGTATTAG CATGCGGCAGTCAAAGGTCGACAAGCTGTATGCTGGCCTGAAGGACCTTGCTGAGGAGAGGAGAGGAAAA CTTGATGAGAGGCACAGGCTGTTCCAGCTCAACAGAGAGGTGGATGACCTGGAACAGTGGATCGCTGAGA GGGAAGTGGTCGCAGGCTCCCATGAGTTGGGACAGGACTATGAGCATGTCACGATGTTACAAGAACGGTT CCGAGAATTTGCTCGAGACACAGGAAACATTGGGCAGGAGCGTGTGGATACAGTTAATAACATGGCAGAT GAACTCATCAACTCTGGACATTCAGATGCTGCCACCATTGCTGAGTGGAAAGATGGTCTCAATGAAGCCT GGGCTGACCTCCTGGAGCTCATTGACACAAGAACACAGATTCTTGCTGCCTCATATGAACTTCATAAGTT TTACCATGATGCCAAGGAGATCTTTGGCCGAATCCAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGA AGAGATCAAAACACTGTGGAAACTTTACAGAGAATGCACACCACCTTTGAGCACGACATCCAAGCTCTGG GCACTCAGGTGAGGCAGCTGCAGGAGGATGCAGCTCGCCTCCAGGCAGCCTATGCAGGGGACAAGGCTGA TGACATCCAGAAGCGTGAGAATGAGGTCCTGGAAGCCTGGAAGTCCCTGCTGGATGCTTGTGAGGGTCGC AGGGTGCGGCTGGTAGACACAGGAGACAAGTTCCGCTTCTTCAGCATGGTGCGTGACCTCATGCTCTGGA TGGAAGATGTCATCCGGCAGATCGAGGCCCAGGAGAAACCACGGGATGTGTCATCTGTTGAACTGTTAAT GAATAATCATCAAGGTATCAAAGCTGAAATTGATGCTCGTAATGACAGCTTTACAGCCTGCATTGAGCTT GGGAAATCCCTGCTGGCACGGAAACACTATGCTTCTGAGGAGATCAAGGAAAAGTTACTGCAGCTGACAG AGAAAAGAAAAGAAATGATTGACAAGTGGGAAGACCGGTGGGAGTGGTTAAGACTGATTTTGGAGGTCCA TCAGTTCTCAAGGGATGCCAGTGTGGCAGAGGCTTGGCTGCTTGGACAGGAACCATACCTATCCAGCCGT GAAATTGGCCAGAGTGTAGACGAAGTGGAGAAGCTTATTAAGCGCCATGAGGCGTTTGAAAAGTCTGCAG CGACCTGGGATGAGAGATTCTCTGCTCTGGAAAGGCTGACAACGTTGGAGCTACTGGAAGTGCGCAGACA GCAAGAGGAAGAAGAAAGAAAGAGGCGGCCACCTTCTCCGGACCCAAACACGAAGGTTTCAGAGGAGGCT GAGTCCCAGCAATGGGATACTTCAAAAGGAGACCAAGTTTCCCAGAATGGTTTGCCGGCTGAGCAGGGAT CTCCACGGGTTAGTTACCGCTCTCAAACGTACCAAAACTACAAAAACTTTAATAGCAGACGGACAGCCAG TGACCATTCATGGTCTGGAATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009260
Insert Size	6465 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009260.2, NP_033286.2
RefSeq Size	9049 bp
RefSeq ORF	6465 bp
Locus ID	20742
UniProt ID	Q62261
Gene Summary	Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains different segments for its 5' UTR, 5' coding region, 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 2) has shorter and distinct N- and C- termini when it is compared to isoform 1.

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