Cacna1i (NM_001044308) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ca(V)3.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225224 representing NM_001044308 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACAGCAACTTACCACCCTCATCTTCAGCAGCCCCGGACCCTGAGCCGGGAATCACTGAGCAGC CGGGGCCCCGGAGTCCCCCTCCATCCCCTCCAGGCCTGGAGGAGCCATTGGATGGAACCAACCCTGACGT CCCGCATCCAGACCTGGCACCCGTTGCCTTCTTCTGCCTGCGCCAGACCACGAGCCCACGGAACTGGTGC ATCAAGATGGTATGTAACCCGTGGTTCGAGTGTGTGAGCATGCTGGTCATCCTGCTGAACTGTGTGACCC TGGGCATGTACCAGCCATGTGACGATATGGAGTGCCTCTCAGACCGCTGCAAGATCCTGCAGGTCTTTGA TGACTTCATCTTCATCTTCTTCGCCATGGAGATGGTGCTTAAGATGGTGGCCCTGGGCATTTTTGGCAAG AAGTGCTACCTCGGGGACACATGGAACCGCTTGGACTTCTTCATTGTCATGGCAGGGATGGTCGAGTACT CTCTGGACCTACAGAACATCAACCTGTCAGCCATCCGCACTGTGCGTGTCCTGAGGCCTCTCAAAGCCAT CAACCGTGTTCCCAGCATGCGGATTCTGGTGAACCTGCTGCTGGACACGCTGCCCATGCTGGGAAACGTG CTCCTGCTCTGTTTCTTCGTCTTCTTCATCTTTGGCATCATCGGCGTGCAGCTCTGGGCAGGCCTGCTGC GGAACCGCTGCTTCCTGGAGGAGAACTTCACCATACAAGGGGATGTGGCCCTGCCCCCTTACTACCAACC AGAAGAGGACGACGAGATGCCCTTCATCTGCTCCCTGTCGGGGGACAATGGCATTATGGGCTGTCACGAG ATCCCTCCACTCAAGGAGCAGGGCCGAGAGTGCTGCCTGTCCAAAGATGACATGTACGACTTCGGGGCGG GACGCCAAGACCTCAACGCCAGCGGCCTCTGCGTCAACTGGAACCGCTACTACAACGTCTGCCGCACGGG CAACGCCAACCCTCACAAGGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGTGATCTTTCAG GTGATCACTCTGGAAGGCTGGGTGGAGATCATGTACTACGTGATGGATGCACATTCTTTCTACAACTTCA TCTACTTCATCCTGCTCATCATAGTGGGCTCCTTCTTCATGATCAACCTGTGCCTCGTTGTCATAGCGAC CCAGTTCTCTGAGACCAAGCAACGGGAGCACCGGCTGATGCTGGAGCAACGCCAGCGCTACCTGTCCTCC AGCACGGTGGCCAGTTACGCCGAGCCCGGCGACTGCTATGAGGAGATCTTCCAATATGTCTGTCACATCC TTCGCAAGGCCAAGCGCCGTGCTCTAGGCCTCTACCAGGCCCTGCAGAACCGGCGCCAGGCCACGGGCCC CGGGACACCAGCCCCTGCCAAGCCTGGGCCCCATGCCAAGGAGCCCAGCCACTGCAAGCTGTGCCCACGA CACAGTCCCCTGGACACCACTCCCCACACACTGGTGCAGCCCATCTCTGCCATTCTGGCCTCCGATCCCA GCAGCTGCCCTCGCTGCCAGCACGAGGCAGGCAGGCGGCCCTCTGGCCTGGGCAGCACTGACTCGGGCCA GGAAGGCTCAGGTTCTGGCGGTTCTGCAGAGGCTGAAGCCAATGGCGATGGACCCCAAAGCAGTGAGGAT GGGGTCTCCTCAGGTTTGGGGAAGGAGGAGGAACAGGAGGACGGGGCAGCCCGACTGTGTGGGGATGTGT GGCGAGAGACACGGGCCAAGCTGCGGGGCATTGTGGACAGCAAGTACTTCAACAGAGGCATCATGATGGC TATTCTGGTTAACACTGTCAGCATGGGCATCGAGCACCATGAACAGCCAGAGGAACTGACCAACATCCTG GAGATCTGCAACGTGGTCTTCACCAGCATGTTTGCCCTGGAGATGATTCTGAAGCTGGCTGCCTTTGGGC TCTTTGACTACCTGCGGAACCCTTACAACATCTTCGACAGCATCATTGTCATCATCAGCATCTGGGAAAT CGTGGGGCAGGCTGACGGCGGCCTGTCCGTGCTGCGCACCTTCCGGCTGCTGCGGGTGCTGAAGCTGGTG CGCTTCATGCCGGCGCTGCGGCGCCAGCTCGTGGTGCTCATGAAGACCATGGACAACGTGGCCACCTTCT GCATGCTGCTCATGCTCTTCATTTTCATCTTCAGCATCCTTGGGATGCATATCTTTGGCTGCAAATTCAG CCTCCGCACGGACACTGGAGACACAGTCCCTGACAGGAAGAACTTCGATTCCTTACTGTGGGCCATCGTC ACCGTGTTTCAGATCCTCACTCAGGAGGACTGGAACGTTGTCCTGTACAATGGCATGGCCTCCACCACCC CCTGGGCCTCCCTCTATTTTGTTGCCCTCATGACCTTTGGCAACTATGTTCTCTTCAATCTCCTGGTGGC TATCCTGGTCGAGGGCTTCCAGGCTGAGGGTGATGCTAATCGCTCCTACTCAGACGAGGACCAGAGCTCA TCCAACTTGGAGGAGCTTGATAAGCTCCCAGAGGGCCTGGACAGCAGCAGAGATCTCAAGCTCTGCCCAA TACCCATGACACCCAATGGACACCTGGACCCTAGTCTCCCTCTGGGTGGACATCTGGGTCCTGCTGGTGC CATGGGTGCTGCCCCCCGCCTCTCACTGCAGCCAGACCCAGTGCTGGTGGCCCTAGAATCCCGGAAAAGC AGTGTCATGTCACTGGGCAGGATGAGCTATGATCAGCGATCTTTGTCCAGCTCCCGGAGCTCCTACTATG GGCCCTGGGGCCGCAGCGGGACCTGGGCCAGCCGCCGCTCCAGCTGGAACAGCCTGAAACACAAGCCACC CTCAGCTGAGCACGAGTCCCTGCTCTCCGGGGAGCGTGGTGGTAGTTGCGTCAGGGCCTGTGAAGGCGCC CGGGAGGATGCGCCACCTCGCGCTGCACCCCTGCATGCCCCGCACACCCACCACGCGCATCATGGACCCC ACTTGGCACACCGTCACCGCCACCACCGTCGGACACTGTCCCTTGATACCAGGGACTCTGTTGACCTGGC AGAGCTGGTGCCCGTGGTGGGTGCCCACTCGCGGGCCGCTTGGAGGGCGGCAGGCCAGGCCCCTGGGCAC GAGGACTGCAATGGCAGAATGCCCAACATAGCCAAGGATGTCTTCACCAAGATGGACGACCGCCGCGACC GTGGGGAGGACGAGGAGGAGATCGACTACACCCTGTGTTTCCGCGTCCGCAAGATGATTGATGTGTACAA GCCTGACTGGTGCGAAGTCCGCGAGGACTGGTCGGTCTACCTCTTCTCCCCTGAGAACAAGTTCCGGATC CTGTGTCAGACCATTATTGCTCACAAGCTTTTTGACTACGTGGTCTTGGCCTTCATCTTCCTCAACTGTA TCACCATTGCTCTGGAGAGGCCCCAGATCGAAGCTGGTAGCACTGAGCGCATCTTCCTCACGGTGTCTAA CTACATCTTCACAGCCATCTTTGTGGGCGAGATGACACTGAAGGTGGTCTCTTTGGGCCTATACTTCGGT GAGCAAGCATACTTGCGTAGCAGCTGGAACGTCCTGGATGGTTTCCTGGTCTTTGTGTCTATCATCGATA TCGTGGTATCCGTGGCCTCTGCAGGGGGAGCCAAGATCCTGGGGGTCCTCCGGGTCCTGCGGCTCCTGCG TACCTTACGTCCTTTGAGGGTCATCAGCCGGGCCCCTGGGCTGAAGCTGGTGGTAGAGACGCTCATCTCC TCCCTCAAGCCCATTGGAAACATTGTCCTCATCTGCTGCGCCTTCTTCATCATCTTCGGTATCCTGGGGG TGCAGCTTTTCAAAGGCAAGTTCTATCACTGTCTGGGAGTGGACACCCGAAACATCACCAACCGATCAGA CTGTGTGGCCGCCAACTACCGCTGGGTGCATCACAAATACAACTTTGATAACCTGGGCCAGGCTTTGATG TCCCTCTTTGTCTTGGCCTCCAAGGATGGCTGGGTGAACATCATGTATAATGGATTGGATGCTGTTGCTG TGGACCAGCAGCCGGTGACAAACCACAACCCCTGGATGCTGCTGTACTTCATCTCCTTCCTGCTCATCGT CAGCTTCTTCGTGCTCAACATGTTCGTGGGAGTGGTCGTGGAGAACTTCCACAAGTGCCGGCAGCATCAG GAGGCTGAGGAGGCACGGCGGCGTGAGGAGAAACGGCTACGGCGCCTGGAAAAGAAGCGCCGTAAGGCTC AGAGGCTGCCCTACTATGCCACCTACTGTCCCACAAGACTGCTCATCCACTCCATGTGCACTAGCCACTA CCTGGACATCTTCATCACCTTCATCATCTGCCTCAACGTGGTCACCATGTCCCTGGAGCACTACAACCAG CCTACATCCCTAGAGACAGCCCTCAAGTACTGCAACTACATGTTCACCACTGTCTTTGTGCTGGAGGCTG TGCTGAAGCTGGTGGCGTTTGGCCTGAGGCGTTTCTTCAAGGACCGATGGAACCAGCTGGACTTGGCCAT TGTGCTGCTGTCTGTCATGGGCATCACACTGGAGGAGATTGAGATCAATGCGGCCCTGCCCATCAACCCC ACCATCATCCGTATCATGCGTGTTCTGCGTATTGCCCGGGTGTTGAAGCTGTTGAAGATGGCCACAGGAA TGCGGGCCCTGCTGGACACAGTGGTACAGGCTCTGCCCCAGGTGGGCAACCTGGGCCTCCTCTTCATGCT GCTCTTCTTCATCTACGCCGCTCTGGGAGTGGAGCTCTTCGGAAAGCTGGTCTGCAATGACGAGAACCCG TGTGAGGGCATGAGCCGGCACGCCACCTTCGAGAACTTCGGCATGGCCTTCCTCACACTCTTCCAGGTCT CCACAGGCGATAACTGGAATGGGATTATGAAGGACACTCTGCGAGACTGCACCCACGACGAGCGCAGCTG CCTGAGCAGCCTGCAGTTTGTGTCGCCTCTCTACTTTGTGAGCTTCGTACTCACAGCGCAGTTTGTGCTC ATCAACGTGGTGGTGGCCGTGCTGATGAAACATCTGGATGACAGCAACAAGGAGGCCCAGGAGGATGCAG AGATGGATGCTGAGATCGAGCTGGAGATGGCCCATGGCCTAGGCCCCGGGCCCGGCCCCTGTCCCTGTCC CTGTCCCTGCCCCTGCCCCTGTCCCTGTCCTGGCCCGAGGATGCCCACCAGTTCACCGGGGGCTCCAGGC CGAGGATCAGGAGGGGCAGGCGTTGGAGGCGACACTGAGAGCCACCTGTGCCGGCACTGCTATTCTCCAG CCCAGGAGACCCTGTGGCTGGACAGCGTCTCTTTAATCATCAAGGACTCCTTGGAGGGGGAGCTGACCAT CATTGACAACCTGTCTGGCTCCATCTTCCACCACTACTCCTCACCTGCCGGCTGTGACAAGTGTCACCAT GACAAGCAAGAGGTGCAGCTGGCTGAGACAGAGGCCTTCTCCCTGAACTCAGACAGGTCCTCGTCCGTCC TGCTGGGGGATGACCTGAGTCTTGAGGACCCCACGGCCTGCCCGCAGGGCCCCAAGGAGAGCAAGGGTGA GCTAGAGCCTCCGGAGCCCATGCAGGCTGGAGACCTGGATGAATGCTTCTTCCCCTTTGCCGGTGAGCCA GTGTCTGCAGGCCCAGAGAGCCTGCTGTGCGAGATGGGGGCCATTCCATTCAACCCCGTCCAGTCCTGGC TCAAACATGAGAGCAACCAAGCACCCCCAAGCCCTTTCTCCCCGGATGGTTCCAGCCCTCTGCTGCAGAT GCCTGCTGAGTTCTTCCACCCTGCTGTGTCTGCCAGCCAGAAGGGGCAGGAACCGGGCATGAGTTCAGGG ACCCTGCCCAAGATTGCACTTCAGGGGTCCTGGGCATCCCTGAGGTCACCGAGTGTCAACTGCACCCTCT TGCGCCAGGCTACTGTGAGTGACACGTCTTTGGATGCCAGCCCTAGCAGCTCAGCGGGCAGCCTGCAGAC CACACTGGAGGACAGTCTGACCCTGAGTGACAGTCCTCGGCGTGCCCTGGGACCACCAGTCCAGGTGCCA GGGCCACGGGCTAGCCTGTCACCTGCCACCCGGCGCCGCCTCAGCCTGCGGGGGCGTGGCCTGTTTAGTC TGCGTGGGCTGCGGGCCCATCAGCGTAGCCACAGCAGCGGTGGCTCCACCAGCCCTGGCTGCACTTACCA CGACTCCATGGACCCCTCTGATGAGGAGGGCCGCGGGGGCGCAGGTGGTGGGGGCGCGGGCAGTGAGCAC TCCGAGACCCTCAGCAGCCTGTCACTCACATCTCTCTTCTGCCTACCACCCACGCTGCCACCACCCGGTC TCACCCCAGCCAGGAAGTTCAGCAGTACCAGCAGCCTGGCTGCTGGACCTGGCCGTCCTGGCGCCACTGT CTCGGTCCGCGGCCTGGCCAGGAGTCCCTCATGGGCTGCAGACCGCAGCAAGGACCCACCAGGCCAGGCA CAGCTGGCCTCCGGCTTCGGCTCCTCAGCACCCGAGCCACAGCCACCTCCCGGGGAGTCAACGGATGCGG CGAGCAAAAGGAAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001044308
Insert Size	6600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001044308.2, NP_001037773.2
RefSeq Size	9833 bp
RefSeq ORF	6600 bp
Locus ID	239556

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