Polq (NM_001159369) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A430110D14Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225250 representing NM_001159369 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCTTCCGCGCCGGAGTAGGAAACGGCGGCGTTCATCGTCCGGCTCCGACACGTTCTCGGGAGATG GTGATAGCTTTGTTAGCCCTCAGCTCCGGTGTGGACCGGTGCTGAGTCCACCTCCGGGGCTGGGACGCGG CCGGAGGCTCACAGGGACAGGAACAAACAAGCGAAGAGTTTCTGATGACCAGATAGACCAGCTGCTGTTG GCAAATTGGGGACTTCCTAAAGCAGTTCTGGAGAAATACCATAGTTTTGGTGTAAGAAAGATGTTCGAAT GGCAGGCAGAATGCCTTTTGCTTGGTCACGTCTTGGAAGGAAAGAATCTAGTTTATTCAGCTCCTACAAG TGCTGGGAAGACTCTTGTTGCAGAGTTACTTATTCTGAAGCGGGTTTTGGAAACACGGAAGAAAGCGTTG TTTATTTTGCCCTTTGTGTCTGTGGCTAAAGAGAAGAAATGCTACCTCCAGAGTCTGTTTCAGGAAGTAG GACTAAAGGTAGATGGCTATATGGGCAGCACCTCACCAACGGGACAGTTCTCCTCCTTGGACATTGCTGT TTGCACTATTGAGAGAGCCAATGGGCTGGTCAATCGCCTCATTGAGGAAAATAAGATGGATCTGTTAGGT GAAAGTATCCTAGTTTGTAAGAACTCTGAGAAATCCAAGGGCATTGCTCTGCTTCAAGGATCTCTGGAGC CTGTGCACAGCTGTCTGCAGAGACAGGGAGAAGTCACTGCTAGCATGATACGAGCTATTCTGGAGATAAT CGTTGGTGGGGTGGCCAGTACGTCACAAGATATGCAGACTTATGCCGCCTGCACCTTCTTGGCTGCTGCT ATACAGGAAGGGAAGCAAGGGATGCAGAGAAATCAAGATGATGCTCAGCTTGGAGCGATTGATGCCTGTG TGACGTGGCTGTTAGAAAATGAGTTCATCCAGGTGGCAGAGCCCGGGGACGGCACAGGAGGAAAGGTGTA TCACCCAACACATCTTGGATCAGCTACTCTTTCTTCTTCACTGTCTCCAACTGATACCCTTGATATTTTT GCTGACCTCCAAAGAGCAATGAAAGGCTTTGTTTTAGAGAATGACCTTCATATTGTCTACCTGGTTACAC CTGTGTTTGAAGATTGGATTAGTATTGATTGGTACCGATTTTTCTGTTTATGGGAAAAATTACCAACTTC TATGAAAAGGGTGGCAGAGCTGGTTGGAGTGGAGGAAGGGTTCTTGGCTCGATGTGTGAAAGGAAAAGTA GTAGCTAGAACTGAGAGACAGCATCGGCAGATGGCCATCCATAAAAGGTTTTTCACAAGTCTTGTGTTAC TGGATTTAATCAGTGAAATTCCCTTAAAGGACATTAATCAGAAGTATGGATGTAATCGTGGACAGATTCA GTCTTTGCAACAGTCAGCTGCTGTCTATGCAGGAATGATTACGGTGTTTTCCAACCGCCTGGGCTGGCAC AACATGGAACTACTGCTTTCCCAGTTTCAGAAGCGTCTTACGTTTGGCATCCAGAGAGAGCTGTGTGACC TCATCCGGGTGTCCTTACTCAACGCGCAGAGAGCCAGGTTCCTGTATGCCTCTGGCTTTCTCACCGTGGC AGATCTTGCCAGAGCTGATAGTGCGGAGGTAGAGGTGGCTCTGAAGAACTCTTTGCCTTTCAAAAGTGCC CGGAAGGCAGTGGATGAAGAAGAGGAAGCAGCTGAAGAACGGCGGAGTATGAGAACTATTTGGGTGACTG GCAAGGGCTTATCTGCAAGGGAAGCAGCAGCCTTAATAGTGGAAGAAGCTAAAATGATTCTACAGCAGGA CTTAATTGAAATGGGAGTACGGTGGGATCCGAAGTCACCCTTGAGTTCTAGCACACACTCACGGACCAGC ACGTCAGAAGTAAAGGAACACACGTTCAAATCCCAGACTAAGAGTTCTCATAAAAGGTTAGCATCAATGG GCAGAAATAGCATTAGAGCAAGTGGGTCTAATGACAAGCCGTCCCCAGATGCAGAGCGAGGCATAGATGA CTGCAGCGAGCATGCAGACTCTCTCTGTAAGTTCCAGGGCAATTTCGAGCCCCAGACACCCTCCATTTGT ACAGCAAGAAAACGGACTTCTTTAGGTATAAACAAAGAAATGCTTCGAAAGTCTCTGAAAGAAGGAAAGC CAAGCACTAAGGAAGTTCTTCAAACTTTCTCATCGGAGAAAACAAGAAAAACTGCTTTGAGTTTTAGTTC AGAGCAAGTGAACAACACTTTACCATCTGGGAGAGACAGAAAGTATCAGAAAAAATCTTGGGGCAGTAGT CCTGTGCGTGACTCTGGGATGCATAGAGGTGATTTACAGGGACAGACTATGTGTACATCTGCTCTATGTG AAGACTCACAGAAGTCCTTAGAGGAGCAGAATGCAGAATTTAGAAGTCCAGGGCTATTTGCAAAACATCT ACCTTCTTGTGCCAAAGAAAAATGCAAGAAGCCCTCACTCCCTTTACAAAGGCAGCAGGCTTGCTCAAGG AGAAGCACAGAGAGCTGTGCTGCTGTGGGGCATCCCGCTGCAGGGTCGTCCCCTGCTGCTGCTAGAGACA GAAGAGGACTGGCTGCTCGGGAGACAGAAAAGGGAAATGAGGCGCTCACGGAGAATGGTGGAGAAAGCCA GCTCCAAGACACCTACCCAGTCAGCCAGTACCTGGAATACCACAGTGAGAAACACACAAACACTTGCACC AGACAGAAGACATTAACAGAGGGACAAGCTGGTAGTAGTTATGTGGCCAGAGACTCAAATGATGCTGCAC CCATCAAATGTGAACGTATGAAGCTTAATAGCAAAGACCGCGACTCAAATCCTTGTCGTCAGGCATTGGG AAGTTACACAGGCAGGACTGAGGCACTTCAGTCAACTGCAAAACTTGGCCAAGCAGGAGGCCAGTGTGAA AATCTTCTAAATTCTTCTGGAGTACAAGGAAAAACAGGTGCTCATGCAACAAATAGAACTGAACATAGTC ATGCTTCTAACCCAGCCTTCTGTGACTTTGGAGATAGCTTAGACTTGGATACTCAGTCAGAGGAAATAAT TGAGCAGATGGCAACTGAAAATACCATGCAAGGAGCAAAAGCAGTAGTGATAATGGAAGAGGGTTCCGCC ATGCAGAACAAGTGTCACAGCACTCCAGGTGACCAGCATGTCCCAGGAGCAGCCAACACAGACCATGTGG ACAGTAAGAAGGTTGAGTCTGTGAAAGCAAACACCGAAAAGAACATCAATAGAGGCGCTCCAGTCAGCCT CATCTTCCATACTCAAGGGGAAAATGGCGCTTGCTTTAAAGGGAATGAGCATTCTGTTACTGATTCCCAA TTAAACAGTTTTCTTCAAGGGTTTGAAACACAAGAAATAGTGAAGCCAATCATACCGCTGGCTCCTCAAA TGAGAACTCCTACTGGTGTGGAGGAAGAAAGTCTGCCTGAAACCAGTTTGAATATGAGTGATAGTATACT ATTTGACAGTTTTGGAGAAGATGGCTTCGGACAAGGACAGTCACCTGATATAAAGGCAAATCAGCCACTC CTTTCTGAAATGACACCAAACCATTTCAGCAATCCTCCACACCCACAAGAAGACCCAGTTATGACACCAA CTGTGAGTGAGCCTCAGGGTACTCAGCAGCAAGGAGTTTGTCTCAGTGGTGAATCTATTATATTTTCAGA CATAGACTCTGCTCAGGTCATTGAAGCCTTAGACAATATGGCTGCATTTCATGTGCAAGAGAACTGTAAC TCAGTAGCCCTTAAAACATTAGAACCCAGTGATTCTGCAGTGCTGGGTAATGAGTGTCCCCAAGGAAAAC TGGTCAGAGGAGATCAAAATGAAGGGTCCCCAAAGCCCAAACTCACAGAGACAAATCAAGATAATTCATT CACTTGGTCAGGAGCATCATTTAACTTAAGTCCAGAACTGCAAAGGATTTTAGATAAAGTATCTAGTCCT CGAGAAAATGAAAAGCCCAAGATGATACATGTAAACTTGTCTTCCTTTGAAGGAAACAGCAAAGAGTCAC ATGAAAGAGAGGAAATAAATTCAGATTTGGGGACAGTTCAGAGAACTTCAGTTTTCCCCAGTAATGAAGT TAAAAACAGGACTGAAGGGCTAGAGAGCAAAGCCAGGCATGGTGGAGCCTCATCTCCCTTGCCTCGTAAA GAAAGTGCTGCAGCTGATGACAATGGCCTCATTCCTCCTACACCCGTTCCTGCTTCTGCTTCTAAGGTAG CCTTTCCAGAGATTCTCGGAACATCTGTAAAACGTCAGAAAGCCAGCAGTGCTTTACAGCCAGGGGAAAG TTGTTTATTTGGCTCACCTTCAGACAATCAAAACCAGGATTTGAGTCAAGAGCTTAGAGACAGCCTCAAG GACTATGATGGTTCTGTTGCAGACACAAGTTTTTTTCTGCAGTCTCAGGATGGACTGCTGTTAACTCAAG CCTCATGCAGCTCAGAGAGTCTGGCCATAATTGACGTAGCGAGTGATCAGATTCTCTTTCAGACATTTGT GAAGGAGTGGCAATGCCAAAAGCGCTTTTCCATCTCTCTGGCTTGTGAAAAGATGACAAGTTCAATGTCT TCCAAAACTGCTACCATAGGTGGAAAGTTGAAGCAAGTTAGCTTGCCACAGGAAGCCACTGTTGAAGATG CTGGGTTCCCTGTGCGTGGCTGTGATGGTGCTGTGGTAGTTGGACTAGCAGTGTGCTGGGGTGCAAAGGA TGCCTATTATTTGTCACTGCAGAAGGAACAAAAGCAGTCTGAAATAAGTCCCAGTTTGGCCCCACCACCT CTGGACGCAACACTAACTGTGAAGGAGAGGATGGAGTGCCTTCAGTCCTGCTTGCAAAAGAAGTCTGATC GAGAGCGGTCTGTTGTCACCTATGATTTCATCCAGACCTATAAAGTCCTCCTCCTGTCATGTGGCATCTC CCTGGAGCCCAGTTATGAAGACCCCAAGGTGGCATGCTGGCTTCTCGACCCAGATTCTAAGGAGCCAACT CTTCATAGCATAGTGACCAGTTTTCTCCCCCATGAGCTAGCACTCCTAGAAGGAATGGAGACAGGCCCAG GAATTCAGAGCCTGGGGCTAAATGTGAACACTGAACATTCTGGGAGGTACAGAGCATCTGTGGAGTCTGT TCTCATCTTCAACTCCATGAATCAACTCAACTCTTTGTTGCAGAAGGAAAACCTTCATGATATTTTCTGC AAAGTGGAGATGCCCTCTCAGTACTGCCTGGCCTTGCTAGAACTAAATGGAATTGGCTTTAGTACTGCGG AGTGTGAAAGCCAGAAGCATGTTATGCAAGCCAAGCTGGACGCCATTGAGACCCAAGCCTATCAGCTGGC TGGCCACAGTTTTTCCTTTACAAGTGCAGATGACATCGCACAGGTTTTATTTTTGGAACTAAAGTTACCA CCGAATGGAGAGATGAAAACGCAAGGCAGCAAAAAGACTCTGGGTTCCACCAGAAGAGGGAATGAGAGTG GCCGCAGAATGAGGCTGGGAAGACAGTTCAGCACTAGTAAGGATATTTTAAATAAATTAAAGGGCTTGCA TCCTTTACCAGGCCTGATACTGGAATGGAGAAGAATCAGTAATGCTATTACCAAGGTGGTCTTCCCTCTG CAGCGGGAAAAGCACCTGAACCCCCTTCTCAGAATGGAGAGGATCTACCCGGTGTCACAGTCACACACGG CTACAGGACGGATAACCTTTACAGAACCCAATATCCAGAATGTGCCAAGAGATTTTGAGATTAAAATGCC AACACTAGTAAGGGAGAGCCCACCTTCTCAAGCTCCAAAAGGCCGGTTCCCAATGGCAATAGGACAAGAT AAGAAGGTTTATGGCCTGCACCCCGGGCACAGAACACAGATGGAAGAGAAGGCCTCAGATAGAGGGGTGC CATTTTCAGTGAGCATGCGCCATGCCTTTGTGCCTTTCCCAGGTGGGCTGATCCTGGCTGCCGACTACTC TCAGCTTGAACTGAGGATCCTGGCTCACTTGTCTCGTGACTGTCGTCTCATTCAAGTCTTAAATACGGGA GCGGATGTGTTCAGGAGCATTGCTGCCGAGTGGAAGATGATTGAGCCTGATGCTGTTGGTGACGATCTGA GGCAGCACGCAAAGCAGATTTGCTATGGAATTATATATGGAATGGGAGCTAAATCTTTAGGAGAGCAGAT GGGCATTAAAGAAAATGATGCTGCTTCCTACATTGACTCTTTCAAGTCCAGATATAAAGGGATTAATCAT TTCATGAGGGACACAGTGAAGAACTGTAGAAAAAATGGATTTGTTGAGACAATTTTGGGAAGACGTAGAT ATTTGCCAGGAATCAAAGACGACAATCCTTATCATAAAGCACACGCTGAACGTCAGGCCATCAACACAAC AGTCCAGGGATCAGCTGCTGATATAGTCAAAATAGCCACAGTTAATATTCAGAAGCAATTAGAGACCTTC CGTTCAACCTTCAAATCCCACGGTCATCGGGAAAGTATGCTCCAGAATGACCGAACAGGATTGTTGCCCA AGAGGAAACTTAAAGGGATGTTTTGTCCCATGAGAGGAGGATTCTTCATCCTTCAACTTCACGACGAGCT TTTATATGAAGTGGCAGAAGAGGATGTTGTTCAGGTGGCTCAGATTGTCAAGAATGAAATGGAATGTGCT ATAAAACTTTCTGTGAAGCTGAAAGTGAAAGTGAAAATAGGTGCCAGCTGGGGAGAGCTGAAGGACTTTG ATGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001159369
Insert Size	6798 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001159369.1, NP_001152841.1
RefSeq Size	7732 bp
RefSeq ORF	6798 bp
Locus ID	77782
UniProt ID	Q8CGS6
Gene Summary	DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25275444, PubMed:25642963). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (PubMed:25642963). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates. Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (By similarity). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (PubMed:16222339, PubMed:16172387, PubMed:16890500, PubMed:17449470). However, POLQ does not play a major role in somatic hypermutation (PubMed:18485835).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

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