Spag17 (NM_028892) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4931427F14Rik; PF6; Spag17-ps
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225273 representing NM_028892 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCCAAGAAGGAGAAACCTACCGGGAGCGCAAACTATAAGATATGGGAACCTTCCCTCATAGCTG CACACTTGAACCAGAATGATTGGAAGGCCTCCATCGCCTTCGTGGTTGGGAACCGAGTTGAGGATGATCT TCTCATTCATGCCCTTGACCTGGCTGTTCGACTCCCTCAGCGGAAACTCTTCAGTATTGTGTCATGGGAA GACATTCTGCAGCAGATGGATGAAATACAGTCACTTGCTGAAAGTGCTTCAGCTAAAAAGGGGAAAAAGC CCACATCTGTCAATTTACCGTTACATTACGAGGTGTTCCTGGCAGCAAAGATAATCATGGAGAGTGGAGA GAAACTAACCTTACCATTGATAGGGAAACTCTTGAAGTGTCAACTTCTCCATATTAAATCCAAGGACCAG CAGAGACGAGAAAATGAAAAGAAGATGGTAGAAGAAAGAACAAAATCAGAAAAAGACAAAGGAAAAGGGA AGTCTCCAAAGGAGAAGAAAGTTGCCAGTGCCAAGCCTGGGAAGGGAAAGAGCAAGGACCAGCCTGAGGC CACTGTAACAGTGAGGAAGACCACACAGTTAAAACGGAGGGGAGAAGATGACGAGGCCAAATCCTATATC GATGATGAGCCAGACGATGGTGCCCAATATTACATTATTGTTGTGGGCTTCAACAATCCTCAGCTATTGG CCATTATGACTGAGCTTGGCATTCCTATAACCAGTGTGATTAAAATATCTTCAGAGAACTATGAACCTCT ACAGACACACCTGGCAGCAGTCAGGCAGCAACAGGAAGCTGTCCTTCAGCCAGAAGATATAGAAGCTGAA AAATTGAAGAGAAAAAATTCCATCAAGGAACTTGAGGTTTTCTGGAAGTACTTGGAACCAATCCTGAATA ATGAGAAACTTGAAATACATCTCTTTGATGTTGCTCGTCTCCAGTACATGGTCAAGGCAACATATTTTCC TTCTGATTGGTCAGACAATGAACAGATGCTTGCATTGGGCACCGAGATCTTTGAAAACATTGCCTGTTTG ATGTACGATAGCCTGGACTGGAAGAGGCAACATCATCACTATTTGCAAAGCATGCAGCTCATCAATGTCC CACAGGTGGTCAGTGAGAAGACCGTGCTAGAGGCAATCACAATCCCAGAGCCTCCACCATCAACTGCTCC AGCCCCCACTGGGAAGAAGAAAGCACAGTATGAGGAGTCGCATGCTCCACCAACAGTGGCTTTTATCATC ACAACTGAAGTAGACATGCGGTATTACAATGACCTGCTGAATCCAATTCCAGAGGAATTTATTTCTGTGT CCTTGATACTGCACTGCATGGTGGAACAGGTTGTAGCAACTGAAGAAGATCTCATCCCGCCAAGTTTGGT GGAGCCAGCCCCCAGAGCAGACGGGTTAGACTACAGAATCGCAGCTCACATTGTGTCGATCCTGCCTTCA CTCTGCCTCTCCGAAAAGGAAAAGAAGAATCTTCGTGAGATATTCTTAACTGAAGGAGAGAGTGAAAGCA AAGCCCTGCCCAAAGGTCCCCTCCTACTGAACTACCACGATGCGCATGCCCACAAGAAGTATGCACTGAA GGACCAAAAGAATTTTGACCCGGTTCAAGTTGAGCAGGAGATGCAGTCCAAGCTGCCACTGTGGGAATTC CTTCAGTTCCCTCTCCCCCCTCCATGGAACAGCACTAAACGACTCGCCACTATTCACGAGCTTATGCATT TCTGTACGAACGAAGTTCTGAGCTGGAATGAAGTAGAACGAGCCTTCAAGGTATTTACTTTTGAGAGCCT GAAGCTCTCAGAGGTGGATGAGGAAGGGAGGCTCAAGCCCACAGAAACGACCAGTGACACAGATGTTGAA AATTTCAACATTCCTTGGGACAATCCTGCCAGGTTCGCTAAGCTGATAAGGCAGCGGTACATTCACAGGA TGAGCATGCAGAAGGCCCCGCCAGTAGTAGTAGAGATTGAAAACACTGAGAGAACACTATTTGTGAACAA GAATTTTGCCAAAGCTGAGCAAGATGCACAAGGAGATGAAAATTCCCCAAATTCTGACGAGCCTGATGCT ATTAGTGTGACGGGTTCTACTTCCAACTCCACAAAACCCTGGAATTCTAGTAATAGACAGTTCTCAGAAA AAGAGACCAGTGGGTCCATGTGGCCCCAGCCAGAGTCCATGGATCAGACCATGGACACTGAGATCAAAGA TGACGCAGCCACAAAGGATGATTCTCCTGAAAAAAAACCCAAGAAGATGGTGGTGGAAGCCGACATAGAG GACATTAAAAAAACGCAACAGCGCAGTTTAATGGACTGGAGCTTTACTGAATATTTTCAGCCAAAAGTCC TGCTTCAGGTTCTTCAGGAGGCTCACCAGCAATACCGATGTGTTGACTCTTACTACCACACGCAGGATAA CTCTTTACTTCTGGTTTTTCATAATCCAATGAATCTACAGCGTCTGCAATGTGAACATTGGAATATTGCT CTTCACTCCAACGTCGGATTCAGGAATTACTTAGAACTTGTTGCAAAATCTATTGAAGACTGGGTCACAC AGGAAGAAGCGAAGTACCAGGAAGCCAAAATGGCTGAGGAGCTCAACAGGATCAGGATTGAGTTGGAACT GAAAGCCACTGTCAAAACTTCTGCTAGCAAAATTCCTGGCCCCAAAAGATCTAAAACTAACAAAGTATCC AGCAAAACAGAGCTATCAGACCAAGAAAAAGATAAAGAAAAGGAAAAAGACAAGATCCCCTTCGTATTAG AAGGCTCTCTCAAGGCATGGAAGGAAGAACAAGAGAGGTTGGCTGAGGAGGAACGCCTGAAGGAGGAAAA GAAAGCAGAGAAGAAGGGAAAGGATACAGGGAAAAAGAAAGGCAAGGATAAGGCCGACAAAGACGATGCT AAGGCTTTGAAGAAAAAGTCTTCTTCCAAGGAGAAGCCCAAAGAAGAGCCAGCAAAGACGCTGGAAGTGA TAGAGGAAACCGCCCCACTTCCGGTACCGGAAGTCGTTTATCCGTTCCGAGGATACAATATGGGAGACAT ACCTATCCAAATCTCAGGAACAAATTACTATCTGTATCCTTCTGATGGTGGGCAGATTGAAGTGGAAAAG ACCAGGTTTGAACGAGGGTCAACTTTCATCAAAGTCAAAGTAAAAAAAGATAAACACAATTTTATAATTC ACCTGAAAGACCCAAAGGAAATTGTGAAAAAGGAAAAGGAAGAGAAGAACTCAGAAGAGGAGGAGGAGGA GGAAGAGGAGAAGGAGGAGGTAGAAGAGAAGAAGCCGAAGGAGGGGGAAGAGGAAAAGGTTAAACAGAAA GTGGAAATGAAAAGAGCACAGATAGAGAAGGAAGCTGTCAGCAAGTTTGGATCTTTTTCTGCCACCTTGG AAAATGGAATCTGCCTCTCGATAAGTTACTATGGATCAAATGGAATGGCACCAGAGGTTATAAATTCTGA GTTGGAAGCAATGATGAATATTCCTTCAGCTATGACAGCAACAGTGGTTCCTGCTGTAGTGACTGTTCCC CAAGGCAAAGGGAAAGCTAAGCCGAAAGGCAAAGAAAAACACAAAGATTCCATTAAAGAAGAGGAACTTC CAAAAGAAGAAGAGAAGAAGAATCACATACAGGAAGAAGTAGAACCAGAGATCGTTATACAGGAATCACC ACCCTATGTACCCACCTTCCAAAACTTAAATGTATCTTGCCCCAGTGGGCTCCTGCTGACCTTCATTGGG CAAGAATCTACAGATTATTCCATCGTAGATGAAGAACCCACCCGGAACCTCATGATCCGCCAGAGCTACC CCCAGCGGTTGAAGCACTATGAGTTCTATAAAGCAGTGATGCCACCCCTGGAGCAGGAGGCATCAAGAGT TGTCACCAGCCAGGGCACCGTTATCAAGTATATGCTGGATGGATCCACACAGATTCTCTTTGCAGATGGT GCTGTGAGCAGCAGCCCTGATTCGGGTCCTGTTTACACATCTCCTGAATTGCCTACAAGCCCCCATAATG GAGATTTGGTGGACAGTGCCTCTCAGCCCAAATCAGAAACAGGACCTGAGATCATCATTACTAAGAAAGG GAAAGGTCACAAAAATCAAACAGTGGCAAATAAGAGTGAAACCCATGATATTATCCCAGAGGTACCTCCA CCAACTCCCGTAGAGTCCCACATTGGCACCTGGTTTACCACAACACCTGATGGAAGGAGGATTGGCACCA AAGGACTAGAGAAAATTGAGGACTTGAAACCATATTTGTTCTTCCAGGCTACAGATCCCATCAATGGAAC GGTCATGACTACTCGAGAAGACAAGGTGATCATTGTTGAGAAGAAAGACGGGACTCGGGTTGTGGACCAT GCTGATGGTACCAGAATCACAACCTTTTACCAAGTGTATGAAGATCATATTACTCCCTCAAATGATGAAG AAACAACTGAAGGTCCAAGGACTGTCACTAGACAGGTGAAGTGTATGCGGATAGAAAGCTCACACTATGC CACTATTATCACCAACTGCGAGGACAGTAGCTGCTGTGCCACCTTTGGGGATGGAACATCTATTATTGCA AAGCCACAGGGGTCATACCAGGTGCTACCTCCAAACACAGGCTGCCTTTATATTGATAAAGACTGCTCAG CTACATACTGCCATGAATCAAGCAATAATTTGTACCATCCCTTCCAGAAGCGTGAGCAGCTGAGAGCCAG CAGGTATATCATGAAGCACACCTCAGAGGTGATCTGTGAGGTCCAGGATCCTGAGGGGAACACCTTTCAG GTCATGGCTGATGGCTCTGTATCAACAACATTACCTAAAAAGAAACTAGAAGATGACTTCAATGTACAGA TGGAGGGCTACGAAAGCTTATCGTCACTTCACCTTGAAAAGAATCACATGCAGATCTATGGTGAACATGT TCCCAGATTTTTTGTTGTCTATACTGATGGATCGGGAGTGGAACTTCTCCGAGACAGTGACATAGAAGAA TACTTATCCTTGGCGTATGGAGAGTCAACGACTGTGGTTCTCCAGGAACCAGTACAGGAATACCCAGGTG CACTGAGCATCACAGTCCTTCGCCCTTTCCATGAAGCATCACAATGGATCATGAAGAAGGAATTAGACAC CATAGTTCCTCCAAATCTCCAGTCAAGGTCATGGGAAAGGTTTCCTTCAGTTGAGAAAAAGACTCCAGGA CCACCATTTGGCACTCAAATTTGGAAAGGCCTCTCTATTGGGTCTAAGCAGCTGACAAACATCCCAGCCC CTATACTCGAAGGACCCAAAGTGCTACAGATGCGTCAGTTCATTCAGCATGAAGTCATAAAGAATGAGGT GAAACTAAAGCTACAGATTTCCCTTAAGGACTATATAAACCACATCCTGAAGAAAGAAGATGAGCTACAG GAGATGACAGTCAAGGATTCCAGAACCGAGGAGGAGAGGGGCAATGCTGCAGATCTCCTCAAGCTGGTTA TGTCTTTCCCGAAAATGGAGGAAACTACAAAAAGTCATATGACTAAAGTTGCAGCCCACCTAACAGTTTT ATTCAAGCAATCTATGGCTTCGGCTCCAAAATGTTCACCAGACAGCTACTCTAAAGAATTCCTTGAAAAG AAGTGGAGGAGTCTGTCACAGGGAACGAGCTGGAAAGAAAAGCTTGAGCAGCAGAGGAACAATATCAAGA AAACACAAAGCTACCTAATGCAAATTAAGACAAAAGAAGTAACACCTTATTTTAAGTCCGAATTGAGCTC GTTATTTAAGTCAAAGTATGATTATCTGGAGAAGTTTTCCAAAAGCCTGCCTCCTTTTGTGAAGAAAAAT GAGGCCAAACTCATGACAGCTGTTCCGGATCTCTACTCGGATTCTACACTGACCGTAGATACAGAAAAAG AAGCCTCAAATACACATCCCCTTCTAAACCAGGAGGTTGCTGAGAATATCCAAGAGTCTCCTAGGGAAAA AGAAACGGAATACGTAAACAAATCTCTCCAGACCTCTTCTTCCCAAAACCAATATGAAAATGTAACAATA TCTCCCAAGGAGTCTGTTTATCAAAGTCAAACTGAAATTGAAACCAAAGATACCAAGGAATCTGCTATCC AAAACTTCACTGAAAAGTTTAAGAAATATACCAAGAAGTCTGCTAGCCAAAACGAAATTGAAGATCTAAT AAAATCTACCAAGGAGTCTGTTAGCCAAAGACAAACTGAAAATGTCACCAGACCTCCTACTGAAGAGCCA GACATTTATATGCCAATCAAAATTCCAACCCAGTCATTGCTGCAGGATGTTACTGGACAAGCAAGAAAAG AGAAAGTGAGGTTACCTTATTACATGATGAGCTCCAAGCCCAAATCTCAGCCTTATGCCAAGGTGGAAGA TCCTGTTGGGGGAAGAGTGAATACATCCTCCATCGCATCTGCTGCTATGTATAACCCAAACGCATCCCCT TTCGGGTTCCACCTTCTCCCACCATCTGTGAAGTTTGGAGTGCTTAAAGAAGGACACACCTATGCGACCA TTGTGAAACTCAAGAATGTCGGCGTGGACTTCTGCAGGTTTAGAGTGAAGCAGCCCCCACCCAGCACTGG ACTGAAAGTGACATATAAACCTGGGCCTGTGGCTGCTGGTTTGCAAGCAGAGCTAAAGGTAGAGCTTTTT GCCATGGCTGTGGGTGAAGACGGTGCCAAGGGCTCAGCACACATCTCCCACAATATCGAAATCATGACGG AGCATGATGTTCTGTTCTTACCTGTGGAAGCAACAGTTTTAACAAGCAGTAACTATGACAATCGACCAAA AAATCTTCCCCAGGGAAAAGAAAATCCCATGGTCTTCAGGACTTCCACAATCTCCTCCTCTTCACTTGGA GTCGTCATGTCTCAAAAGGCCACTCATCATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_028892
Insert Size	6963 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_028892.4, NP_083168.3
RefSeq Size	7862 bp
RefSeq ORF	6963 bp
Locus ID	74362
UniProt ID	Q5S003
Gene Summary	Component of the central pair apparatus of ciliary axonemes. Plays a critical role in the function and structure of motile cilia (PubMed:23418344, PubMed:15827353). May play a role in endochondral bone formation, most likely because of a function in primary cilia of chondrocytes and osteoblasts (PubMed:26017218).[UniProtKB/Swiss-Prot Function]

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