Spg11 (NM_145531) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6030465E24Rik; A330015I11; C530005A01Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225315 representing NM_145531 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCAGCCAGGGCCCGGAAGTGCTGCCTCCCCCGGCTGTGCCGGGGCCATGGAGCGGGTCCTGC CCATGCTGCTGGTGCGGATCCCAGCTGAGGAGACGGCGCAGCTGGGACCGCGGGCGCAGCTGCACCGAGA GCTGGAGGCCCTGGGGAGCCTGACGGCTGCGGGCAGTCTCCAAGTGCTTTCCTTGGCGCCGGGCAGCAGG GGTGGGAGCAGCTGCTGCCTCCCAGGACCCTTTAGGCAGTTCCTATGGGAGGAGTCTCAGAACAGCACAT CAAGAGACAGGCCCAAGCTGCTTGTTGCCAGAGAAGATTATGAGCTGCTCGTATATGAATTTGACTTGAG AGATGGAAGATGTGACGCAGCCTTATTACACGGCTGTTGTGGGAGGACACTGCAGAAGCTCACTGAAGAT CAAGGTGTCAGTATTTCCTTCAAGTCTTTGAGAATTCTGTCATTTCACAACAACACGTCATTACTGCTCA TCAACAGATGTCTCATCCTACGCGTTGTGTTTCCGGGGAAAGAACCTGGGGTCCCAGTGCTGGACTGCCT CTCACTGCCCCTGCCTGCACAGGCAGCGGACATGATCATTGATGCACAGCTCTGTGAGAGATTTCTTTTT GTTCTGAGCACAGTGGGCTTGATCTATATTTTTAACACTATGGATGGCACGCAGGTAGCGCAAGTGGACC TGGCTCTTCTCAGAGAAGATTCTGAGCAGCTGGAGCCCGCCAGTGTCTCTTCCTTTACATCGCTGAGAGT GTCTCAGGACTTGGATGTTCTGGTGCTGGTCAGCTCAGCCAGTACTGCAGTTGCTCTTAACCTACATTTG TATTTCAGGCAACATCCGGGACACCTACTGTGTGAAGGAACACTGGAAGACCTTCCTATTGAAGGGCCTC CAGGGATAGATGAAGACGACCTGGTTAACTCTGCCCACAACATGAAACTGAGCAAGGTGTCCTTTCAAGT TGATAGGTCCTGGAGAGCCCAGCTGTTGTCACTGAATGAATCAGTGAGGGGCTCGGAACCAGAGGTTTCC TGCTGTGCTCCGTGGTTCCAGAGTGCTTTGCGTTTGGAGTCCCTGGAATCTGCTGACCACACCCCCACTG TGCCAACCCACGTTTTCATTCCCGGGGATGTCCCACGTGGCCGGTGTGCTTTTCCTCAGAAGGAGCACGT GAAGAGCAGTGATCCAGGAAGGCCGTGGAAGACAATGCACCTGAGTGAACATGAGCAGCCCACGGAGCTC ACGTGCCTGTCTGTGACGGGATTTACTGCACTGTTTACCTGGGCAGTAGGAGCGACCAGCTGCACCATCG GCCTCTGGGACTTGGAGACCCAGAGCATGCAGTGTTTTTCTCTCAGCCAGAAGTGTACTCCTGTAGACAT TGGCGGAGACCAGCAGCTGTGCCTTGCTTTGACAGATGATGGTCTGTCTCTGATTTTGTTTGGTTTAACC CAAGAGGAGTTTTTGAACAGACTAATGATCCACGGCAGCGCCAGCACTGTGGACTCCCTTTGTCATCTCA ACGGTTGGGGACGGTGCTCAATCCCTATCCACGCTCTAGAGGCTGGCATAGAAAATCGTCAGCTGGACAC AGTAGATTTCTTCTTGAAGAGCAAGGAAAATCTTCTTACTCCGTCTTCACAATCTCCTGCTCCTGATCAG CAGCATCCCTTTTCATCCCATCTGTACCTAAGACAGGTAGAAGAGATGAGCCCAGCCCTGGATTTACTAT GCTCAGCAATTAGAGAAAGTGACTCGGAAACCCAAAGCAAACACTTTGCTGAGCAGCTGCTTCACCTCAC ACTGTCCTTCCTTAACAAACAGATAAGGGAGCTTTGTGTTCACACTGAAGAGCTAGATGAGCATTTTCAG AAAGGAGTGGCCATTTTGACCAGCTACATCAATGAGCTTCGGACTTTTATGATAAAGTTTCCTTGGAAAC CAGGAGATGCCATAGATGAATCTGATGTAAATGAAGGTGTCGTCACAGTGAAGGAGGACCGGGTTTGGGA GGAACTCAGCTTTGAGGAAGTTATTGCTGATGCCATTTTAAATAACAGAATACCAGAGGCACAAACTTTC TTTAGGATTAGTGGTCATTCCGCTCAACGACTGGAGGAACTGGTTAGGATCGGCCTAGATCTGGCATTTG ACAGTTTAAAAAAGAACAACGTGGAGGAGGCCTCGAGGCTCCTGAGGAACATGGGCTTCAGTGTGGAAGA TGAGTTGCTTAAGATATGCTTTTATACAACTGATAAAAATATACGGGACTTTTTGGTTGAAATTTTAAAA GAAAAGGAATGTTTTTCTGAAAAAGAAAGAAGAACTGTAGACTTTGTTCATCAACTTGAAGCACTTTATT CTGGACACTTCCAGGAAAATGCACAGACGCAGGCTTTCCCCAGGTATTGGATAAAGGAACAGGACTGTTT CAAACACAAATCTGTCTTGGACACATTCCTCAAATATGATAAAAAAGATGAGTTTCACAAACAGGACCAT AGAATTGCATTGAATTGGGCTCACCGGTGGGATCTCCAAACACAGGAATGTATCCTTCTCCGCAGACTAA GTCCAGAAGAATACAAAGCGCGCTCCCCGGAAGCCCTGTGGAGACACCTCACGGCTCGCCATGACTGCTC AAGCATCAGCTTGTGGATGGAAGAATTTCAGACCCAGGAAACCGAGTCACCTCAGCAGAGCAAGTGGCCC CCTCTGAGTGCTGACATCATTGAGCAGGGCACTTGCTGCCACAGTCACATGAGGAATGAGATTTTAGACA AGCTGGCCAGAAGTGGCATTTTTCTTGCATCTGAGCTGGAAGACTTTGAACGTCTCCTCCTGAGGCTGAG CCGGATTGGGGGCGTAATGCAGGACAGCCTCCCCGTGCAGAGCTACAAATCCCGTGCAGGGTGTGACTTT CATTCCCGGTTCATCCTCTACTGTTTAGAGCACGGCCTACAGCATCTTCTCTACACCTATCTTGACTATT ACAAACTTAGTCCTGGAAATTGTCCTTTTTTGGAAAAGAAAGAACTACATGAAGCTCACCCTTGGCTTGA ATTTTTGGTTCAATGTCGGCAAGTCTCCAGTAACTTGACAGATCCCAAACTCATCTTTCAGGCTAGCCTT GCAAATGCTCAGATTTTGATTCCCACCAATCAGGCCAGTGTGAGCAGTATGCTGTTGGAAGGACACACCC TCCTGGCCCTTGCTACCACCATGTATGCTCCAGGGGGTGTCAGCCAGGTCATTCAGAATGAAGACAGCGA GAACTGTTTGAAGAAAGTGGACCCCCAGCTACTGAAGGTGGCTTTAACACCTTACCCAAAGCTCAAAGCT GCGCTCTTCCCACAGTACACTGCTCCTAGTATTCTGCCCTCTGATATTACACTCTACCACCTCATCCAGT CCTTGCCGCCCTTTGATCCTAGCCGGCTGTTCGTCTGGCAGTCTGCCAACACACTAGCTATAGGAGACAC AGGCAGTCAGCTCCCACACTTTTCCAGCCCTGACCTGGTGAGTAAATACGCTGTGCTGGAACGGCTGAAC TACGCGTATTATCTACATCACGGACGCCCGTCGTTTGCCTTTGGCACCTTTCTGGTCCAGGAGTTGATCA AGAGTAAGACTCCCAAACAACTGATCCAGCAAGTAGGCAAGGAGGCCTATACCTTAGGGCTCTCTTCCTT CACCAACCCTTCGGTAGGGGCTGCCTGTGTCTGCTTCTTAGAATTGCTTGGCCTCAGCAGCCTCAAGCTC AGAGTTGACCTGAAAATGGCAAATGTAATTTTGGGCTCCAAGCGTAGAGATGAAGATGCTCGTTCCAGCT TCATCAGAGAGTCTCTGGCTGAGAAGCTGTCTAAGCTTGCTGACGGTGACAGAGCAGCCACAGAAGAGCT GCTTGTTCTCTTGGAGGAAGGCGTGTGGGACAGCATTGAGCAGCAGGGCTTTAGCAGGTTGTCCAGTGAG TCCAGCAGCCAGTGGGCATTAGTGCTTCAGTTCTGCATGCTCCATGACAGGAAGCTGAGCGTGTCCTACC TTAGAGAATGTGCCAAAGCCAATGACTGGCTGCAGTTCCTGGTCCACAGCCAGCTGCATAACTACCACCC AGCAGAGGTGGAATCTCTTCTTCAGTATTTCAGTCCTGTCCTTCAGAGCCACTTGAAGCTGGCTTCTGAG AAACTGTCATCAGGGTCCATCTCTAGGGATGACAGCTGCCTCCAGGAACTCCAGAAAAACAAAGGAGAAA CGAGTAATTTCTTTGAAATTCTCCACCGGTGCTCAGATGAGTCCACCTCTTGGAGCTGGCTCCTGGCTGA GGCAGTGAGACATCGGGCTCCCATCCTCAGTGTCCTGGCCTCGTGTGTGCAGGGTGCCAGTGTTGTCTCC TGTCTCTGTGTTTGGATTGTCACCTCCGTGGAGGACAAGGTTGCAGCTGAAGCAATGGGACACATTCAGA TCTCTGTAGAGGACCATCACTGGAGCCTGAAGGACCTGTCCATCATCTGGAGAACTGTATTGACAAGACG CAAGAGCCACACTCTCATCCGAGGTTTCCAGCTCTTTATTAAGGATTCCCCACTTCTATTGATTATGGAG ATGTATGAACTGTGTATGTTCTTCAAGAATTACGAGAAAGCCAAAGTTAAACTTTTGGAGTTCCAGAAAA GCCTTGAAACACTTGACACAGTGGCCGCAAGGGTCCTTCCTATCATACCTGCCTCGTGGATGAAAGACCA GGTTTGTTTCCTTTTGAAACTTATGCCGCAGCAGTGTGAGACGCAGTATGAGCTGGGGAAACTCCTCCAA CTGTTTGTTGGCACTGAGCAGCTCTTCTCTGACGGGCCAGACGTGCAGAAGCTGTGTCTCCTCAGCCAAG TGCTGAAGGACACACCTATCGCCATTAGCCCTGCAGTCATTTCCAGCTACAGCACTGAGAACTTTCAGCG GGAATGTAGATCTATTTTGGAGAAGCTAAAAGCAGATGGGCAGTTTGCTGTGGCTAGGAGAGTGGCCGAG TTAGCAGCCCTGCCTGTGGACAGCTTGCTGATTGAACAGTTAACTCAGGAAATGCAAACACTAACACACA CTCCGCAGTGGTCCCTGAAACAAGAAAGACTTGGCTTCTGGAAAAAATGCCATGAAATTTTTAAGAAAAA CTCTATTTCAAAGAGAGCAGCCTCTTCCTTCTTCTCTAGCCAAGCCCCCGAGGTGAGTGAGCACCCTGCT GAACAGGGCAGCCTTGAGGAGCGCCATCTGCTGCTCACTCTGGCTGGCCACTGGCTTGCCCAAGAAGAGC CGGTTCCCGTGGAGGAACTGGAGGGGCTGGAGAAACAGATTTGGATCTGCCGCGTCGCCCAGCACACATG TGGAGGGGCTGAAGAGGAAGCCAAGCCCAGCCTCTCTCAACACAAGTTGGCTGCTGCTGAACTTTCTTTT GATAGCTTAGCCAGTGAGCTGTCCTTCTCCAAGCTCGCTGCTTTGAACACATCCAAATACTTAGGCCTTA ATGATCTTCCATCCAAGACGACGTGTGAGAACAGACTGAAACACAAAGAGCAGGAGTCACTGAACACTTT GATCGGGCAGCTGCTGGATGGTGGCTGTGTGCATGAAGCAAGCAGAGTGTGCCAGTATTTCCGTTTCTAT AGCCAGGATCTAGTGCTGGTGTTGCACTGCAGAGCCTTGGCCTCCGCAGAAGCCACCATGGAAGACTTGC ACTCAGAGATCCGTGCCCTCCTCTCAAGTGCCGCACAGCCTGAGGACCTGGAGAGCCCGAGTGTCCCTCT TCGGAAAGCCCATAGCTCTTCAAGTTTGGACAGTCAGTCATTTGTGATGGTACCACCCACTGATGAGGTA GCCCAGAACCTCCACACTCTCATAAGCAAGTGCCTCCATGGGAAGAACTACTGCCGGCAGGTCCTCTGTC TGTATGAACTTGCCAAGGATTTGGGCTGTTCCTATGGAGATGTTGCTGCTAGGGACAGTGAAGCCATGCT CCGAGCAATCTTGGCTTCTCAGCGGCCTGACCGATGTAGACAAGCCCAGGTCTTCATCAACACCCAGGGC CTTGAAGCAGACACTGTGGCTGAACTAGTGGCTGAAGAGGTGACACGGGAGCTACTGACTCCATCAGAGG GAACAGGAGAAAAGCAACCTTTCAACCCAGCAGAGGAAAGCCAGACATTTCTTCAGCTAACTGCTTTGTG TCAAGACCGTACATTGGTAGGCATGAAGTTGTTGGACAAGATTCCCTCTGTCCCCCACGGGGAGCTGTCT TGCACCACAGAGCTCCTGATCCTGGCCCACCACTGCTTTACCTTCACGTGCCACATGGAAGGCATCACCA GAGTCCTGCAAGCCGCCCGCATGCTCACAGATAACCACTTGGCTCCCAATGAGGAGTACGGATTAGTGGT CCGCCTCCTCACTGGCATCGGGCGCTACAATGAGATGACGTACATATTTGACTTGCTGCATCAAAAGCAT TATTTCGAAGTTCTGATGAGAAAGAAGCTAGACCCAACCGGCACCCTGAAGACAGCCCTACTAGACTACA TCAAACGTTGCCGCCCTGGAGACAGCGAAAAGCACAATATGATTGCCCTGTGCTTCAGTATGTGCCGGGA GATAGGAGAGAATCACGAGGCAGCAGCCTGCATCCAACTGAAGCTGATTGAGTCTCAGCCCTGGGAGGAA AGCCTCAAGGATGGAGCCCAACTAAAACAGCTCCTGCTTAAAGCCCTGACTCTGATGTTGGATGCAGCAG AGAGTTACGCCAAGGACTCCTGTGTTCGACAGGCCCTGCACTGTAACCGGCTCACCAAGTTGATAACACT ACAGATTCACTTTCTCAACAGTGGCCAGAATACAATGCTTATCAACTTGGGCCATCAGAAGCTAATGGAC TGTATCATGACCCTACCTCGGTTCTACCAGGCTTCTATTGTAGCTGAGGCCTATGACTTTGTTCCTGACT GGGCTGAAGTACTCTACCAACAAGTGATTCTTAAAGGAGACTTTAGTTATTTGGAAGAATTTAAGCAGCA GAAGTTACTAAGACCCAATATATTTGAAGACATTTCCAAAAAATATAAACAACACCAGCCTACCGACAGG GTCACAGAAAACCTGAAGAAGTTACTTTCATATTGTGAAGATATCTACCTGTATTACAAGTTGGCATATG AACACAAATTTTTTGAAATTGTAAATATGCTTCTGAAGGACCCTCAGACGGGATGCTGCCTTAAAGATAT GCTCGCAGGTTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_145531
Insert Size	7293 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_145531.2, NP_663506.2
RefSeq Size	7665 bp
RefSeq ORF	7293 bp
Locus ID	214585
UniProt ID	Q3UHA3
Gene Summary	May play a role in neurite plasticity by maintaining cytoskeleton stability and regulating synaptic vesicle transport.[UniProtKB/Swiss-Prot Function]

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