Cenpe (NM_173762) Mouse Untagged Clone

CAT#: MC225329

Cenpe (untagged) - Mouse centromere protein E (Cenpe), (10ug)



  "NM_173762" in other vectors (2)

CNY 39,620.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 312kDa; AU019344; BC049989; C530022J18; CENP-E; Kif10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225329 representing NM_173762
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGAGGCTTCAGTGGCTGTCTGTGTTCGTGTGCGACCCCTCAACAGCAGAGAAGAAGAACTTG
GAGAAGCCACCCACATTTACTGGAAAACAGATAAAAACGCTATTTATCAGAGCGATGGGGGTAAATCTTT
CCAATTTGATCGTGTCTTTGACAGTAATGAAACCACGAAGAATGTGTATGAAGAGATTGCGGTGCCCATC
ATCAGCTCCGCCATACAAGGCTACAATGGTACTATATTTGCCTATGGACAAACTGCATCAGGAAAGACAC
ACACGATGATGGGTTCTGAAGATTGTTTGGGAGTTATACCCAGGGCAATTCATGACATTTTCCAAAGGAT
AAAGAAGTTTCCTGAGAGAGAATTTCTCCTACGAGTTTCTTACATGGAGATCTACAATGAAACCATTACA
GATTTACTCTGTAATGCTCAAAAAATGAAACCTTTGATAATTCGGGAAGACACCAATAGGACTGTATATG
TTTCTGATCTCACAGAAGAAGTGGTTTATACAGCAGAAATGGCTTTGAAATGGCTCGCAACAGGAGAAAA
AAACAGACATTATGGAATAACCAAAATGAATCAAAGAAGCAGTCGTTCTCATACGATTTTTAGGATGATT
CTGGAAAGTAGAGAAAAGGCTGAGCCTTCTAACTGCGATGGCTCCGTCAAAGTGTCTCATCTGAATTTGG
TCGATCTTGCAGGCAGTGAAAGAGCTGCTCAAACAGGAGCTGAAGGTGTGCGATTGAAGGAGGGCTGTTT
TATAAATCGGAACTTGTTTATTTTGGGACAAGTGATTAAGAAACTCAGTGATGGACAGGTTGGGGGTTTC
ATAAATTACCGAGATAGCAAATTGACACGAATTCTTCAGAATTCCTTGGGAGGAAATGCCAAGACGCGCA
TCATCTGCACGATCACCCCAGCGTCCCTGGACGAAACCCTGACGACGCTGCAGTTTGCCAGCACTGCTAA
GTATATGAAGAATACTCCTTACGTTAATGAAGTGTCGAATGATGAAGCTCTCTTGAAAAGATACAGAAGA
GAAATAGCGGATCTTAGAAAACAATTAGAGGAGGTAAATACAAAGACTCGGGCACAGGAAATGGAAAAGG
ATCAACTGGCCCAGCTTCTGGATGAAAAAGACTTGCTTCAGAAAGTTCAGGATGAGAAAATTAATAACCT
GAAGCGGATGCTGGTGACGTCATCTTCTATTGCATTGCAGCAGGAGCTAGAGATTAAAAGGAAACGAAGA
GTTACTTGGTGCTATGGCAAAATGAAGGATTCAAACTATGAAAAGGAATTTAAAGTCCCGACAAGCATAA
CAACAAGAAAACGTAAAACTTCTGTGACTTCTTTGAGAGAAAATTCTCTCATGAAGTTCGGAGAGAGTGC
AGCCTCTTCCGAGTTTGAAATGTTGAATAACACTCTTGAGTCATTGGCTGAGGTAGAGTGGAGCTCGGCA
ACAACGCTCCTAAGCGAGGAAAATGTAGAAAGTGAGTTGAACTCACTTAATGCTCAATATAATGATCTGG
TATTAGACTATGAACAGCTCAGAAGAGAAAATGAAGACTTGAAATTGAAATTAAAAGAAAAAAATGAGTT
AGAGGAATTTGAGCTTCTAGAACAAAAAGAAGAAAGAGATCAAGAGATGCAACTAATGCATGAAGTTTCC
AACTTAAAGAATTTAATTAAACACGCAGAAGAATATAATCAAGACCTTGAGAATGACCTAAGTTCAAAAG
TGAAGCTTCTTAAAGAAAAAGAGGAGCAGATTAAGAACCTGCAGGAATACATAGATGCCCAGAAGTCAGA
AAAGATGAAGATAGACCTGTCATACACATCGGACGCCACTGAAGACCTGAAACAGGCGATGCGCACCCTG
TCTGATTTGGACACAGTGGCCCTTGACGCTAAGAAAGAGTCAGCCTTCCTTAGAAGTGAAAATCTGGAGC
TGAAGGAGAAAATTAATGAACTTTCGGATTCCCGTAAGCAAATGGAAAGTGACATTCAGATGTATCAACG
CCAACTGGAAGCAAAAAAGAAAATGCAAACTGATCTGGATAAGGAATTACAGTTGGCTTTTCAAGAAATA
TCAAAACTCAGCGCCCTGGTAGATGGCAAAGGTTTACTGTCTAATTTGGAATTGGAGAAAAGGATTACTG
ATCTCCAAAAAGAACTAAATAAAGAAGCTGAAGAAAAACAAACTTTGCAAGAAGAAGTTAACTTGCTGTC
GGAGTTGAAATCTTTACCGTCTGAGGTGGAAACGCTGAGGAGAGAGCTGTATGAGAAATCTGAAGAGCTC
CATATAATAACCACAGAAAGAGAGAAACTGTTTTCTGAAATGGCTCATAAAGACAGTAGGATTCAAGGTT
TACTTGAGGAAATTGGAAACACTAGAGATGACCTTGCCACTTCCCAGCTGAGTCGGAGGGGCAGTGATGG
AGAATGGCAAGCCCTGGAAAGCCTGCACGCAGAGCTGGAGCACAGACACGCGGGGGTCCTGGAGGAGAGA
GAGAGGCTGAAGCAGGAGATTGGGGCTCTCTCCAAAGAAGCAGAAAGCCTGGCTTTCAGTTTGGATTCCG
TGAAGGCCGAGCTTTCTCACAAGACTCAAGAGCTTGAGCAGAAAACAGTTGAGGGTCAAGAAAGGCTAAA
TAAAATGGAGGCGCTGAGAGAAGAGTTAGAAAGCAGAGACTCCAGCCTGCAGTCTGTAGAGAAGGAGAAA
GTCCTGCTCACTGAGAAGCTGCAGCAAGCCTTGAAGGAAGTAAAAGCCTTAACCCAAGAAAAGAAAAACC
TGAAGCAGCTCCAGGAGAGCCTGCAAACTGAGAGAGACCAACTCAGAAGTGACATTCAGGACACTGTGAA
CATGAACATAGATACTCAAGAGCAGTTACTAAATGCTCTGGAGTCTCTGAAACAACACCAAGAAACAATT
AACATGCTGAAAATGAAAGCTGCTGAGGAACTATCCGATAATTTGCATGTAAAGGACAGGGGAGGAGCTA
GAGATGAAGCTCAGCAAAAGATGGATGGCATAGATGAACAGAATGAGAGTGCCCACACATTGCTTGGTGG
TGGTAAGGACAATGAGGTAACTGAGGAACAGAGGAAAATCGACTCTCTAATGCAGGAGAATAGCGGACTC
CAACAGACACTGGAAAGTGTTAGAGCAGAAAAGGAACAACTGAAGATGGACCTAAAGGAAAATATCGAAA
TGAGTATTGAAAACCAGGAAGAGTTAAGAATTCTTAGAGATGAACTGAAAAGACAACAAGAGGTAGCTGC
ACAGGAAAAGGATCATGCCACCGAGAAGACCCAGGAGCTCTCCAGGACGCAGGAAAGATTGGCAAAAACA
GAAGAGAAGTTAGAGGAAAAGAACCAGAAACTCCAAGAGACACAGCAGCAACTTCTTAGTACACAAGAAG
CTATGAGTAAGCTGCAGGCAAAGGTGATTGACATGGAAAGCTTACAGAATGAATTCAGGAACCAAGGATT
GGCATTGGAGCGTGTGGAGACAGAGAAACTGGAGTTAGCTCAGAGACTTCATGAAAGTTATGAGGAGGTG
AAATCTATAACCAAAGAAAGAAATGATCTAAAGGAATTGCAGGAGTCATTTGAAATAGAGAAGAAGCAAC
TTAAAGAATATGCAAGGGAAATAGAAGCCGCGGGCCTACAAACAAAAGAAGAACTAAACATTGCCCACGC
GAATCTAAAAGAGTACCAGGAAATCATCACAGAGCTAAGAGGAAGCATTTCTGAAAACGAAGCACAAGGA
GCAAGCACCCAGGACACGGCAAAAAGCGCCCCTGAGCTACAAGGAGAGGTTCCAGAGCAAGAGCTGCTTC
CTGTTGTCAAAGAAGCCAGGCACAGTGCAGAAAAAGTGAACGGACTGGAGCCAGTGGGAGCACATTCCAG
AACAGTGCACTCCATGACAATGGAGGGCATAGAAATAGAAAACCTCAGGCTGACCAAGAAACTTGAAGAA
AGTCAGATGGAGATCAGCTGTCTCACTAGAGAGAGAGAAGACCTGAGAAGGACACAGGAAACACTTCAGG
TCGAATGTACCCAGCTGAAAGAAGATGCCAGAAGAACTTTGGCTAATCATCTAGAAACTGAAGAGGAACT
TAACCTTGCTCGTTGTTGCCTGAAAGAGCAGGAGAACAAGATTGACACCCTAATAACGAGCCTCTCGCAG
AGGGAAACTGAGCTGTCCAGTGTGCGAGGACAACTAGCGCTGACTACTGCTGAACTGGAGAGAAAGGTTC
AAGAACTCTGTGAGAAACAGGAAGAACTTACTAGGAAAGAAACTAGCGAGGCTCAAGGGAAGATGAGTGA
ACTAGAGCAACTCAGGGAGCTGCTGCTTGCCCAGGCCTCGGCACTGCAGAACGCAGAGAGTGACAGGCTG
CGGCTGAATACACAACTGGAAGAAAGTCAAGAGGAAATGAAAACTCTAAGGGAGGAAAGAGAAGAGCTGA
GAAGAATGCAAGAGGCTCTTCACGTGGAAAGTGAGCAGCAGAAAGAAAGCATGAAGGAAATCAGCTCAAA
ACTCCAAGAACTACAGAACAAAGAGTATGAATGTCTTGCAATGAAGACTATTAATGAGACTCAAGGAAGC
AGATGTGAAATGGACCACTTGAACCAGCAGCTGGAGGCCCAGAAGTCAACTCTGGAAAAGGTGGAGATGG
AGAATGTAAACTTGACTCAAAGACTTCATGAGACCCTTGAGGAAATGAGATCTGTAGCAAAAGAAAGAGA
TGAGCTTTGGAGCATGGAGGAGCGCCTCACAGTAGAGAGAGACCAACTGAAGAAGAGCCTTGAAGAAACA
GTAACTAAAGGTATGGAAAAAGAGGAAGAGCTAAGAGTTGCGCATGTGCATCTGGAAGAGCACCAGGAAA
CTATCAACAAGCTCAGAAAGATGGTGTCTGACTACACAGATGAAATATCACATACTCAAGGGGACCTAAA
GCACACAAATGCCGTTGTAGAAGCTCAGAACCAAGATCTCCGGGAAAAAGAACATCAACTTTCTCAAGTA
AAAGCTGATCTCAGAGAAACCGTGGATCAAATGGAGCAACTGAAGAAGAAGCTCGAAGCCCAGAGTTCGA
CTCTAGAAAGCAGAGAAATAGAGAAGTTAGAATTGACTCAGCAACTTAACGAAAACCTTAAAAAAATAAC
ACTTGTCACTAAGGAAAATGATAGCCTAAAAATTATGGATGAAGCCCTCAGGGAAGAACGAGACCAGCTA
CGGAAAAGTCTTCAGCAAACAGAGGCAAGGGATCTGGAAAATCAAGAGAAACTAAGAATTGCTCACATGA
ATCTAAAAGAGCACCAAGAAACTATTGACCGACTCATGGAGACTATGTCTGAGAAGACAGAGGAAATATC
AAATATGAAAATGGAGTTAGAAAATGTAAACATGAAATTACAAGAAAAGGTTCAAGAACTTAAGACAAGT
GAGCGTCAACGTGTTAAGTTAAAAGCAGATGCCAGTGAAGCCAAGAAGGAGCTCAAGGAGCAGGGCTTAA
CTCTGAGTAAGATAGAGATGGAGAACTTAAATCTCGCTCAGAAGATTCATGAGAACCTTGAAGAAATGAA
ATCTGTAAGAAAAGAAAGAGATGATCTAAAGAAACTTGAGGAGATACTCAGAATGGAGAGAGACCAGCTC
AAGGACAACCTGAGAGAAGCCATGCTTAAGGCCCACCAGAACCATGAAGAGACAATGAAATGTGGAAAGG
GGTTACTGTGTGCTGGAGAATACTGTACAGGAAGACTTCGAGAAAAGTGCTTCAGGATAGAAAAACTCCT
GAAAAGATACTCAGAAATGGCTAATGATTATGAGTGCTTGAATAAAGTCTCTCTTGACTTGGAGAGAGAA
ACTAAAACCCAAAAAGAGCTGTCAGTTACTGTAAGAACAAAGCTCTCACTTCCGCACACACAGACCAAAG
AGATGGAAAAGCTTCTGACTGCAAACCAGAGATGTTCCCTGGAGTTCCACAGAGCCCTCAAGAGACTTAA
GTACGTGTTAAGCTCCATTGCGAGGATAAAGGAAGAGCAGCATGAGTCCATCAATAAACGTGAAATGGCT
TTTATTCAGGAAGTGGAAAAGCAGAATGAACTGCAGATTCAAATCCAGAGCCTTTCACAGACTTACCGTA
TTCCAGCCAGAGACTTACAGATCAAACTGAGCCAGGAGATGGACCTCCATATTGAGGAAATGCTCAAAGA
TTTTTCAGAAAATGATTTCCTCACCATAAAAACTGAAGTCCAACAAGTATTAAATAATAGGAAAGAAATA
ACTGAGTTTTTGGGAAAATGGCTAAATACTCTTTTTGATACAGAAAATCTTAAGAGCACCATCCAGAAAG
AAAACAAAAGCATTGGGCTCGTGAATAACTTCTATCACAGCAGAATCACTGCCATGATAAATGAATCAAC
GGAGTTTGAGGAAAGAAGTGCTACCAGATCCAAAGACTTGGATCAGTACCTGAAGTCACTGAAAGAAACA
ACTGAGCAACTGTCTGAAGTCTACCAGACACTGACCGCCTCCCAGTCTGTTGTGCATCTCCATCCTACCG
TGCAGCCTTCCACACGGGACAGTGAGCGTCCTCAGGCTGCATCAGGAGCTGAACAACTGACCTCAAAGAA
CAAAATTGCTTTAGGAGCTGTGCCCTATAAAGAAGAAATTGAGGATCTCAAAATGCAGCTGGTGAAGAGT
GACCTAGAGAAGAAGGCGACCGCCAAGGAATTCGATAAGAAAATTCTCTCTCTAAAAGCCACTGTTGAAC
ATCAAGAAGAAATGATAAGGCTACTGAGAGAAAATCTTAGAGGACATCAACAGGCCCAGGATACCTCAAT
GATCTCAGAACAAGACTCTCAGCTTCTGAGTAAACCTCTAACCTGTGGAGGTGGCAGTGGAATTGTACAA
AGCACAAAAGCTCTTATTTTGAAAAGTGAATATAAAAGGATGGGAAGTGAGATTTCTAAGTTAAAGCAGC
AAAATGAGCAGCTAAGAAAGCAAAATAATCAACTATTAAGTGATAACTCTCAACTCTCCAATGAGGTTAA
AACCTGGGAGGAAAGAACTCTTAAAAGAGACAGTTACAGAGAAACAACTTGTGAGAATTCTCCAAAGTCT
CCTAAAGTGACTCGGACAGATTCTAAAAGGAGACAGAACACAACTTCTCAGTGCAGGGCACAAAATCTCC
AGGATCCTGTGCCAAAAGATTCCCCAAAATCATGGTTTTTTGATAACCGGTCAAAGTCTCTTCCAGCACC
TCACCCGATCCGCTATTTTGACAACTCCAGTCTAGGGCTGTGCCCAGAACCAGATGACGTAGAGAATGTG
GAGCCTAAGACAGACCTCTGCCAAGCCTCCTTGGAAAAAGATGTGTCCCAGTGCAAGACGCAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_173762
Insert Size 7416 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173762.4, NP_776123.3
RefSeq Size 7813 bp
RefSeq ORF 7416 bp
Locus ID 229841
UniProt ID Q6RT24
Gene Summary Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (By similarity). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:12925705). The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC (PubMed:12361599). Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (PubMed:12925705).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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