Fasn (NM_007988) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A630082H08Rik; FAS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225339 representing NM_007988 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGTGGTGATAGCCGGTATGTCGGGGAAGTTGCCCGAGTCAGAGAACCTACAGGAGTTCTGGG CCAACCTCATTGGTGGTGTGGACATGGTCACAGATGATGACAGGAGATGGAAGGCTGGGCTCTATGGATT ACCCAAGCGGTCTGGAAAGCTGAAGGATCTCTCCAAGTTCGACGCCTCCTTTTTTGGGGTCCACCCCAAG CAGGCACACACAATGGACCCCCAGCTTCGGCTGCTGTTGGAAGTCAGCTATGAAGCAATTGTGGATGGAG GTATCAACCCAGCCTCACTCCGAGGAACGAACACTGGCGTCTGGGTGGGTGTGAGTGGTTCAGAGGCATC CGAGGCCCTTAGCAGAGATCCCGAGACGCTTCTGGGCTACAGCATGGTGGGCTGCCAGCGTGCAATGATG GCCAACCGGCTCTCTTTCTTCTTCGACTTCAAAGGACCAAGCATTGCCCTGGACACAGCCTGCTCCTCCA GCTTGCTGGCACTACAGAATGCCTACCAGGCCATCCGTAGTGGGGAATGCCCCGCGGCCCTTGTGGGTGG GATCAACCTGCTCCTGAAGCCGAACACCTCTGTGCAGTTCATGAAGCTGGGCATGCTCAGCCCGGACGGC ACCTGCAGATCCTTTGATGATTCAGGGAGTGGATATTGTCGCTCTGAGGCTGTTGTAGCAGTTCTGCTGA CTAAGAAGTCCCTGGCTCGGCGGGTCTATGCCACGATTCTGAATGCCGGCACCAATACAGATGGCAGCAA GGAGCAAGGTGTAACATTCCCCTCTGGAGAAGTCCAAGAACAACTCATCTGCTCTCTGTATCAGCCAGCT GGTCTGGCCCCGGAGTCGCTTGAGTATATTGAAGCCCATGGCACGGGCACCAAGGTGGGTGACCCCCAGG AACTGAATGGCATTACTCGGTCCCTGTGCGCCTTCCGCCAGGCCCCTCTGTTAATTGGCTCCACCAAATC CAACATGGGACACCCTGAGCCTGCCTCTGGGCTTGCAGCCCTGACCAAGGTGCTGTTATCCCTGGAGCAT GGGGTCTGGGCCCCTAACCTGCACTTCCACAACCCCAACCCTGAGATCCCAGCACTTCTTGATGGGCGGC TGCAGGTGGTCGATAGGCCCCTGCCTGTTCGTGGTGGCAACGTGGGCATCAACTCATTTGGCTTCGGAGG CTCCAATGTTCATGTCATCCTCCAGCCCAACACACGGCAGGCCCCTGCGCCCACTGCACACGCTGCCCTT CCCCATTTGCTGCACGCCAGTGGACGCACCTTAGAGGCAGTGCAGGACCTGCTGGAACAGGGCCGCCAGC ACAGCCAGGACCTGGCCTTTGTGAGCATGCTCAATGACATTGCGGCAACCCCTACAGCAGCCATGCCCTT CAGGGGTTACACTGTGCTAGGTGTTGAGGGCCGTGTCCAAGAAGTGCAGCAAGTGTCCACCAACAAGCGC CCACTCTGGTTCATCTGCTCAGGGATGGGCACGCAGTGGCGCGGGATGGGGCTGAGCCTCATGCGCCTGG ACAGCTTCCGTGAGTCTATCCTGCGCTCCGATGAGGCTGTGAAGCCGTTGGGAGTGAAAGTGTCAGATCT GCTGTTGAGCACAGATGAGCGCACCTTTGATGACATCGTGCATGCCTTTGTGAGCCTCACTGCCATCCAG ATTGCCCTCATCGACCTACTGACTTCTGTGGGACTGAAACCTGACGGCATCATTGGGCACTCCTTGGGAG AGGTTGCCTGTGGCTATGCAGATGGCTGTCTCTCCCAGAGAGAGGCTGTGCTTGCAGCTTACTGGCGAGG CCAGTGCATCAAAGATGCCCACCTCCCGCCTGGATCCATGGCAGCTGTTGGTTTGTCCTGGGAGGAATGT AAACAGCGCTGCCCCGCTGGCGTGGTGCCTGCCTGCCACAACTCTGAGGACACCGTGACCATCTCTGGAC CTCAGGCTGCAGTGAATGAATTTGTGGAGCAGCTAAAGCAAGAAGGTGTGTTTGCCAAGGAGGTACGAAC AGGAGGCCTGGCTTTCCACTCCTACTTCATGGAAGGAATTGCCCCCACATTGCTGCAGGCTCTCAAGAAG GTGATCCGGGAACCACGGCCGCGCTCGGCTCGATGGCTCAGCACCTCTATCCCTGAGGCCCAGTGGCAGA GCAGCCTGGCCCGCACATCTTCTGCCGAGTACAATGTCAACAACCTGGTGAGCCCTGTGCTCTTCCAGGA AGCACTGTGGCACATCCCTGAGCATGCCGTGGTGCTGGAGATTGCGCCCCACGCACTGTTGCAGGCTGTC CTGAAGCGAGGCGTGAAGTCCAGCTGCACCATCATTCCCTTGATGAAGAGGGATCATAAAGATAACTTGG AGTTCTTTCTCACCAACCTTGGCAAGGTGCACCTCACAGGCATCAATGTCAACCCTAACGCCTTGTTCCC ACCTGTGGAGTTCCCGGCTCCCCGAGGGACTCCTCTCATCTCCCCTCACATCAAGTGGGACCACAGTCAG ACTTGGGATGTCCCGGTTGCTGAGGACTTCCCAAACGGCTCCAGCTCCTCCTCTGCTACAGTCTACAGCA TCGACGCCAGTCCTGAGTCGCCCGACCACTACCTGGTAGACCACTGCATTGACGGCCGGGTCATCTTCCC TGGCACTGGCTACCTGTGCCTGGTGTGGAAGACACTGGCTCGCAGCCTGGGCTTGTCCCTAGAAGAGACC CCTGTGGTATTTGAGAATGTGTCGTTTCATCAGGCCACTATACTACCCAAGACAGGAACCGTGGCGCTGG AGGTGAGGCTGCTAGAGGCCTCCCATGCCTTTGAGGTGTCTGACACTGGCAATCTGATTGTGAGCGGAAA AGTGTACCTGTGGGAAGACCCGAACTCCAAGTTATTCGACCACCCAGAAGTCCCAACACCCCCTGAGTCT GCATCGGTCTCCCGCCTGACCCAGGGAGAAGTATACAAGGAGCTGCGGCTGCGTGGCTATGATTATGGCC CTCAGTTCCAGGGCATCTGTGAGGCCACCCTTGAAGGTGAACAAGGCAAGCTGCTCTGGAAAGATAACTG GGTGACCTTCATGGACACAATGCTGCAGGTATCCATTCTGGGTTCTAGCCAGCAGAGTCTACAGCTACCT ACCCGTGTGACCGCCATCTATATCGACCCTGCCACCCACCGTCAGAAGGTGTACAGGCTGAAGGAGGACA CTCAAGTGGCTGATGTGACAACGAGCCGCTGTCTGGGCATAACGGTCTCTGGTGGTATCCACATCTCAAG ACTACAGACGACAGCAACCTCACGGCGGCAGCAAGAACAGCTGGTCCCCACCTTGGAAAAGTTCGTTTTC ACACCGCACATGGAGGCTGAGTGCCTGTCTGAGAGCACTGCCCTGCAGAAGGAGCTGCAACTGTGCAAGG GTCTGGCACGGGCTCTGCAGACCAAGGCCACCCAGCAAGGGCTGAAGGCGGCAATGCTTGGGCAAGAGGA CCCTCCACAGCACGGGCTGCCTCGACTCCTGGCAGCTGCTTGCCAGTTGCAGCTCAACGGGAACCTGCAG CTGGAGCTGGGAGAAGCGCTGGCTCAAGAGAGGCTCCTGCTGCCAGAAGACCCTCTGATCAGTGGCCTCC TCAACTCCCAGGCCCTCAAGGCCTGCGTAGACACAGCCCTGGAGAACTTGTCTACTCTCAAGATGAAGGT GGCAGAGGTGCTGGCTGGAGAAGGCCACTTGTATTCCCGAATCCCGGCACTGCTCAACACCCAGCCCATG CTACAACTGGAATACACAGCCACCGACCGGCACCCCCAGGCCCTGAAGGATGTTCAGACCAAACTGCAGC AGCATGATGTGGCGCAGGGCCAGTGGAACCCTTCCGACCCTGCGCCCAGCAGCCTGGGTGCCCTTGACCT TCTGGTGTGCAACTGTGCATTAGCCACCCTGGGGGATCCAGCCTTGGCCCTGGACAACATGGTAGCTGCC CTCAAGGAAGGTGGTTTCCTGCTAGTGCACACAGTGCTCAAAGGACATGCCCTTGGGGAGACCCTGGCCT GCCTACCCTCTGAGGTGCAGCCTGCGCCCAGCCTCCTAAGCCAGGAGGAGTGGGAGAGCCTGTTCTCGAG GAAGGCACTACACCTGGTGGGCCTTAAAAGGTCCTTCTACGGTACTGCGCTGTTCCTGTGCCGGCGAGCC ATCCCACAGGAGAAACCTATCTTCCTGTCTGTGGAGGATACCAGCTTCCAGTGGGTGGACTCTCTGAAGA GCACTCTGGCCACGTCCTCCTCCCAGCCTGTGTGGCTAACGGCCATGGACTGCCCCACCTCGGGTGTGGT GGGTTTGGTGAATTGTCTCCGAAAAGAGCCGGGTGGACACCGGATTCGGTGTATCCTGCTGTCCAACCTC AGCAACACATCTCACGCCCCCAAGTTGGACCCTGGCTCTCCAGAGCTACAGCAGGTGCTAAAGCATGACC TCGTGATGAACGTGTACCGGGACGGGGCCTGGGGTGCCTTCCGTCACTTCCAGTTAGAGCAGGACAAGCC CAAGGAGCAGACAGCGCATGCCTTTGTAAACGTCCTCACCCGAGGGGACCTCGCCTCCATCCGCTGGGTC TCCTCCCCCCTGAAGCACACGCAGCCCTCGAGCTCAGGAGCACAGCTCTGCACTGTCTACTACGCCTCAC TGAACTTCCGAGACATCATGCTGGCCACGGGCAAGCTGTCCCCTGATGCCATTCCAGGTAAATGGGCCAG CCGAGACTGCATGCTCGGCATGGAGTTCTCAGGCCGGGATAGGTGTGGCCGGCGTGTGATGGGGCTGGTT CCTGCAGAAGGCCTGGCCACCTCAGTCCTGCTATCATCTGACTTCCTCTGGGATGTACCCTCCAGCTGGA CCCTGGAGGAGGCGGCCTCTGTGCCCGTCGTCTATACCACTGCTTACTACTCGTTAGTGGTTCGCGGGCG CATCCAGCGTGGGGAGACCGTGCTCATCCACTCAGGTTCAGGTGGTGTGGGCCAAGCGGCCATTTCCATT GCCCTCAGTCTGGGCTGCCGCGTCTTCACCACTGTGGGCTCTGCAGAGAAGCGAGCATACCTCCAGGCCA GGTTCCCTCAGCTTGATGACACCAGCTTTGCCAACTCGAGGGACACATCATTTGAGCAGCACGTGTTACT GCACACAGGTGGCAAAGGGGTCGACCTGGTCCTCAACTCACTGGCAGAAGAGAAGCTGCAGGCCAGTGTG CGGTGCTTGGCTCAGCATGGTCGCTTCTTAGAGATTGGCAAATTTGATCTTTCTAACAACCACCCTCTGG GCATGGCTATCTTCTTGAAGAACGTCACTTTCCATGGGATCCTGCTGGACGCCCTTTTTGAGGAGGCCAA TGACAGCTGGCGGGAGGTGGCGGCACTCCTGAAGGCTGGCATTCGTGATGGAGTCGTGAAGCCCCTCAAG TGCACAGTGTTTCCCAAGGCCCAGGTGGAAGATGCCTTCCGCTACATGGCTCAGGGGAAACACATTGGCA AAGTCCTTGTCCAGGTACGGGAGGAGGAGCCTGAGGCTGTGCTGCCAGGGGCTCAGCCCACCCTGATTTC TGCCATCTCCAAGACCTTCTGCCCAGCCCATAAGAGTTACATCATCACTGGTGGCCTAGGTGGCTTTGGC CTGGAGCTGGCCCGGTGGCTCGTGCTTCGCGGAGCCCAGAGGCTTGTGCTGACTTCCCGATCTGGAATCC GCACCGGCTACCAAGCCAAGCACATTCGGGAGTGGAGACGCCAGGGCATCCAAGTGCTCGTGTCAACAAG CAACGTGAGCTCACTGGAGGGGGCCCGTGCTCTCATCGCCGAAGCCACAAAGCTGGGGCCCGTTGGGGGT GTCTTCAACCTGGCCATGGTTTTGAGGGATGCCATGCTGGAGAACCAGACCCCAGAGCTCTTCCAGGATG TCAACAAGCCCAAATACAATGGCACCCTGAACCTTGACAGGGCAACCCGGGAAGCCTGCCCTGAGCTGGA CTACTTTGTGGCCTTCTCCTCTGTAAGCTGCGGGCGTGGTAATGCTGGCCAAACTAACTACGGCTTCGCC AACTCTACCATGGAGCGTATATGTGAACAGCGCAGGCACGATGGCCTCCCAGGCCTTGCCGTGCAGTGGG GTGCCATTGGTGACGTGGGCATTGTCCTGGAAGCGATGGGCACCAATGACACAGTCATCGGAGGTACGCT GCCTCAGCGCATCTCCTCCTGCATGGAGGTACTGGACCTCTTCCTGAATCAGCCCCACGCAGTCCTGAGC AGCTTTGTGCTGGCAGAGAAGAAAGCTGTGGCCCATGGGGACGGGGACACCCAGAGGGATCTGGTGAAAG CTGTAGCACACATCCTAGGCATCCGAGACCTCGCAGGTATTAACCTGGACAGCACGCTGGCAGACCTCGG CCTGGACTCGCTCATGGGTGTGGAAGTTCGTCAGATCCTGGAACGAGAACACGATCTGGTGCTGCCCATG CGTGAGGTGCGGCAGCTCACGCTGCGGAAACTTCAGGAAATGTCCTCCAAGACTGACTCGGCTACTGACA CGACAGCCCCCAAGTCCAGGAGTGACACGTCTCTGAAGCAGAACCAACTGAACCTGAGCACACTGCTGGT GAACCCTGAGGGTCCTACCCTAACCCAGCTCAACTCGGTGCAGAGCTCTGAGCGGCCTCTGTTCCTTGTG CACCCCATTGAGGGTTCCACCACCGTGTTCCACAGTCTGGCTGCCAAGCTCAGTGTGCCCACCTACGGCC TGCAGTGCACCCAAGCTGCCCCCCTGGATAGCATTCCGAACCTGGCTGCCTACTACATAGATTGCATCAA GCAAGTGCAGCCTGAGGGACCCTACCGCATAGCTGGGTACTCATTTGGAGCCTGTGTAGCCTTCGAGATG TGCTCCCAGCTGCAGGCCCAGCAGGGCCCAGCCCCGACCCACAACAACCTCTTCCTGTTTGACGGCTCAC ACACCTACGTGTTGGCCTACACCCAGAGCTACCGGGCAAAGATGACCCCAGGCTGTGAAGCCGAGGCCGA GGCTGAGGCCTTATGCTTCTTCATAAAGCAGTTTCTTGATGTGGAACACAGCAAGGTGCTGGAGGCCCTG CTGCCACTGAAGAGCCTGGAAGATCGGGTGGCTGCCTCCGTGGACCTTATCACTAAGAGTCACCACAGCC TGGACCGCCGAGAGCTGAGCTTTGCTGCCGTGTCCTTCTACCACAAGCTCCGGGCAGCTGATCAGTATAA GCCCAAGGCCAAGTACCATGGCAACGTGACACTGCTGCGTGCCAAGACAGGCGGCACCTATGGCGAGGAC TTGGGTGCTGACTACAACCTCTCCCAGGTGTGTGACGGGAAGGTGTCTGTGCACATCATTGAGGGTGACC ACCGCACACTGCTGGAGGGCAGTGGCCTGGAATCCATCATCAACATCATCCATAGCTCCCTGGCTGAGCC ACGAGTGAGTGTACGGGAGGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007988
Insert Size	7515 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007988.3, NP_032014.3
RefSeq Size	9938 bp
RefSeq ORF	7515 bp
Locus ID	14104
UniProt ID	P19096
Gene Summary	Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.[UniProtKB/Swiss-Prot Function]

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