Polq (NM_029977) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A430110D14Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225353 representing NM_029977 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCTTCCGCGCCGGAGTAGGAAACGGCGGCGTTCATCGTCCGGCTCCGACACGTTCTCGGGAGATG GTGATAGCTTTGTTAGCCCTCAGCTCCGGTGTGGACCGGTGCTGAGTCCACCTCCGGGGCTGGGACGCGG CCGGAGGCTCACAGGGACAGGAACAAACAAGCGAAGAGTTTCTGATGACCAGATAGACCAGCTGCTGTTG GCAAATTGGGGACTTCCTAAAGCAGTTCTGGAGAAATACCATAGTTTTGGTGTAAGAAAGATGTTCGAAT GGCAGGCAGAATGCCTTTTGCTTGGTCACGTCTTGGAAGGAAAGAATCTAGTTTATTCAGCTCCTACAAG TGCTGGGAAGACTCTTGTTGCAGAGTTACTTATTCTGAAGCGGGTTTTGGAAACACGGAAGAAAGCGTTG TTTATTTTGCCCTTTGTGTCTGTGGCTAAAGAGAAGAAATGCTACCTCCAGAGTCTGTTTCAGGAAGTAG GACTAAAGGTAGATGGCTATATGGGCAGCACCTCACCAACGGGACAGTTCTCCTCCTTGGACATTGCTGT TTGCACTATTGAGAGAGCCAATGGGCTGGTCAATCGCCTCATTGAGGAAAATAAGATGGATCTGTTAGGA ATGGTGGTCGTGGATGAGCTGCACATGCTGGGGGACTCTCACCGAGGGTATCTGCTGGAGCTGTTGCTGA CCAAGATTTGCTACGTCACTCGTAAATCAGCATCGCACCAGGCAGAGTCAGCCAGCACTCTGTCTAATGC TGTGCAGATTGTTGGCATGAGTGCTACTCTTCCTAATTTGCAGCTTGTGGCTTCCTGGCTGAATGCTGAA CTTTACCATACCGACTTTCGCCCTGTACCACTTTTGGAATCAATAAAAATAGGAAATTCCATATATGATT CTTCAATGAAGCTTGTGAGAGAATTTCAACCCCTGCTCCAAGTGAAGGGAGATGAAGACCACATTGTTAG TTTATGCTATGAGACCATTCAGGATAACCATTCAGTATTAATCTTTTGTCCATCAAAAAAATGGTGTGAG AAGGTAGCAGATATCATTGCCCGAGAGTTTTACAATCTGCACCATCAACCTGAGGGGTTGGTAAAATCAT CTGAATTTCCACCAGTGATTCTAGACCAAAAGAGCCTTCTGGAAGTGATGGATCAGTTAAAACGTTCACC TTCAGGACTAGACTCTGTGTTGAAGAATACTGTGCCATGGGGAGTAGCGTTTCATCATGCAGGTCTTACT TTTGAGGAGAGGGATATCATCGAAGGAGCCTTCCGTCAAGGTTTCATTCGGGTCTTGGCAGCAACGTCTA CTCTTTCTTCTGGAGTGAATCTTCCTGCTCGTCGGGTGATTATTCGCACTCCAATTTTCAGTGGTCAGCC TTTAGATATCCTCACTTATAAGCAGATGGTTGGGCGTGCTGGCAGGAAAGGAGTAGACACAATGGGTGAA AGTATCCTAGTTTGTAAGAACTCTGAGAAATCCAAGGGCATTGCTCTGCTTCAAGGATCTCTGGAGCCTG TGCACAGCTGTCTGCAGAGACAGGGAGAAGTCACTGCTAGCATGATACGAGCTATTCTGGAGATAATCGT TGGTGGGGTGGCCAGTACGTCACAAGATATGCAGACTTATGCCGCCTGCACCTTCTTGGCTGCTGCTATA CAGGAAGGGAAGCAAGGGATGCAGAGAAATCAAGATGATGCTCAGCTTGGAGCGATTGATGCCTGTGTGA CGTGGCTGTTAGAAAATGAGTTCATCCAGGTGGCAGAGCCCGGGGACGGCACAGGAGGAAAGGTGTATCA CCCAACACATCTTGGATCAGCTACTCTTTCTTCTTCACTGTCTCCAACTGATACCCTTGATATTTTTGCT GACCTCCAAAGAGCAATGAAAGGCTTTGTTTTAGAGAATGACCTTCATATTGTCTACCTGGTTACACCTG TGTTTGAAGATTGGATTAGTATTGATTGGTACCGATTTTTCTGTTTATGGGAAAAATTACCAACTTCTAT GAAAAGGGTGGCAGAGCTGGTTGGAGTGGAGGAAGGGTTCTTGGCTCGATGTGTGAAAGGAAAAGTAGTA GCTAGAACTGAGAGACAGCATCGGCAGATGGCCATCCATAAAAGGTTTTTCACAAGTCTTGTGTTACTGG ATTTAATCAGTGAAATTCCCTTAAAGGACATTAATCAGAAGTATGGATGTAATCGTGGACAGATTCAGTC TTTGCAACAGTCAGCTGCTGTCTATGCAGGAATGATTACGGTGTTTTCCAACCGCCTGGGCTGGCACAAC ATGGAACTACTGCTTTCCCAGTTTCAGAAGCGTCTTACGTTTGGCATCCAGAGAGAGCTGTGTGACCTCA TCCGGGTGTCCTTACTCAACGCGCAGAGAGCCAGGTTCCTGTATGCCTCTGGCTTTCTCACCGTGGCAGA TCTTGCCAGAGCTGATAGTGCGGAGGTAGAGGTGGCTCTGAAGAACTCTTTGCCTTTCAAAAGTGCCCGG AAGGCAGTGGATGAAGAAGAGGAAGCAGCTGAAGAACGGCGGAGTATGAGAACTATTTGGGTGACTGGCA AGGGCTTATCTGCAAGGGAAGCAGCAGCCTTAATAGTGGAAGAAGCTAAAATGATTCTACAGCAGGACTT AATTGAAATGGGAGTACGGTGGGATCCGAAGTCACCCTTGAGTTCTAGCACACACTCACGGACCAGCACG TCAGAAGTAAAGGAACACACGTTCAAATCCCAGACTAAGAGTTCTCATAAAAGGTTAGCATCAATGGGCA GAAATAGCATTAGAGCAAGTGGGTCTAATGACAAGCCGTCCCCAGATGCAGAGCGAGGCATAGATGACTG CAGCGAGCATGCAGACTCTCTCTGTAAGTTCCAGGGCAATTTCGAGCCCCAGACACCCTCCATTTGTACA GCAAGAAAACGGACTTCTTTAGGTATAAACAAAGAAATGCTTCGAAAGTCTCTGAAAGAAGGAAAGCCAA GCACTAAGGAAGTTCTTCAAACTTTCTCATCGGAGAAAACAAGAAAAACTGCTTTGAGTTTTAGTTCAGA GCAAGTGAACAACACTTTACCATCTGGGAGAGACAGAAAGTATCAGAAAAAATCTTGGGGCAGTAGTCCT GTGCGTGACTCTGGGATGCATAGAGGTGATTTACAGGGACAGACTATGTGTACATCTGCTCTATGTGAAG ACTCACAGAAGTCCTTAGAGGAGCAGAATGCAGAATTTAGAAGTCCAGGGCTATTTGCAAAACATCTACC TTCTTGTGCCAAAGAAAAATGCAAGAAGCCCTCACTCCCTTTACAAAGGCAGCAGGCTTGCTCAAGGAGA AGCACAGAGAGCTGTGCTGCTGTGGGGCATCCCGCTGCAGGGTCGTCCCCTGCTGCTGCTAGAGACAGAA GAGGACTGGCTGCTCGGGAGACAGAAAAGGGAAATGAGGCGCTCACGGAGAATGGTGGAGAAAGCCAGCT CCAAGACACCTACCCAGTCAGCCAGTACCTGGAATACCACAGTGAGAAACACACAAACACTTGCACCAGA CAGAAGACATTAACAGAGGGACAAGCTGGTAGTAGTTATGTGGCCAGAGACTCAAATGATGCTGCACCCA TCAAATGTGAACGTATGAAGCTTAATAGCAAAGACCGCGACTCAAATCCTTGTCGTCAGGCATTGGGAAG TTACACAGGCAGGACTGAGGCACTTCAGTCAACTGCAAAACTTGGCCAAGCAGGAGGCCAGTGTGAAAAT CTTCTAAATTCTTCTGGAGTACAAGGAAAAACAGGTGCTCATGCAACAAATAGAACTGAACATAGTCATG CTTCTAACCCAGCCTTCTGTGACTTTGGAGATAGCTTAGACTTGGATACTCAGTCAGAGGAAATAATTGA GCAGATGGCAACTGAAAATACCATGCAAGGAGCAAAAGCAGTAGTGATAATGGAAGAGGGTTCCGCCATG CAGAACAAGTGTCACAGCACTCCAGGTGACCAGCATGTCCCAGGAGCAGCCAACACAGACCATGTGGACA GTAAGAAGGTTGAGTCTGTGAAAGCAAACACCGAAAAGAACATCAATAGAGGCGCTCCAGTCAGCCTCAT CTTCCATACTCAAGGGGAAAATGGCGCTTGCTTTAAAGGGAATGAGCATTCTGTTACTGATTCCCAATTA AACAGTTTTCTTCAAGGGTTTGAAACACAAGAAATAGTGAAGCCAATCATACCGCTGGCTCCTCAAATGA GAACTCCTACTGGTGTGGAGGAAGAAAGTCTGCCTGAAACCAGTTTGAATATGAGTGATAGTATACTATT TGACAGTTTTGGAGAAGATGGCTTCGGACAAGGACAGTCACCTGATATAAAGGCAAATCAGCCACTCCTT TCTGAAATGACACCAAACCATTTCAGCAATCCTCCACACCCACAAGAAGACCCAGTTATGACACCAACTG TGAGTGAGCCTCAGGGTACTCAGCAGCAAGGAGTTTGTCTCAGTGGTGAATCTATTATATTTTCAGACAT AGACTCTGCTCAGGTCATTGAAGCCTTAGACAATATGGCTGCATTTCATGTGCAAGAGAACTGTAACTCA GTAGCCCTTAAAACATTAGAACCCAGTGATTCTGCAGTGCTGGGTAATGAGTGTCCCCAAGGAAAACTGG TCAGAGGAGATCAAAATGAAGGGTCCCCAAAGCCCAAACTCACAGAGACAAATCAAGATAATTCATTCAC TTGGTCAGGAGCATCATTTAACTTAAGTCCAGAACTGCAAAGGATTTTAGATAAAGTATCTAGTCCTCGA GAAAATGAAAAGCCCAAGATGATACATGTAAACTTGTCTTCCTTTGAAGGAAACAGCAAAGAGTCACATG AAAGAGAGGAAATAAATTCAGATTTGGGGACAGTTCAGAGAACTTCAGTTTTCCCCAGTAATGAAGTTAA AAACAGGACTGAAGGGCTAGAGAGCAAAGCCAGGCATGGTGGAGCCTCATCTCCCTTGCCTCGTAAAGAA AGTGCTGCAGCTGATGACAATGGCCTCATTCCTCCTACACCCGTTCCTGCTTCTGCTTCTAAGGTAGCCT TTCCAGAGATTCTCGGAACATCTGTAAAACGTCAGAAAGCCAGCAGTGCTTTACAGCCAGGGGAAAGTTG TTTATTTGGCTCACCTTCAGACAATCAAAACCAGGATTTGAGTCAAGAGCTTAGAGACAGCCTCAAGGAC TATGATGGTTCTGTTGCAGACACAAGTTTTTTTCTGCAGTCTCAGGATGGACTGCTGTTAACTCAAGCCT CATGCAGCTCAGAGAGTCTGGCCATAATTGACGTAGCGAGTGATCAGATTCTCTTTCAGACATTTGTGAA GGAGTGGCAATGCCAAAAGCGCTTTTCCATCTCTCTGGCTTGTGAAAAGATGACAAGTTCAATGTCTTCC AAAACTGCTACCATAGGTGGAAAGTTGAAGCAAGTTAGCTTGCCACAGGAAGCCACTGTTGAAGATGCTG GGTTCCCTGTGCGTGGCTGTGATGGTGCTGTGGTAGTTGGACTAGCAGTGTGCTGGGGTGCAAAGGATGC CTATTATTTGTCACTGCAGAAGGAACAAAAGCAGTCTGAAATAAGTCCCAGTTTGGCCCCACCACCTCTG GACGCAACACTAACTGTGAAGGAGAGGATGGAGTGCCTTCAGTCCTGCTTGCAAAAGAAGTCTGATCGAG AGCGGTCTGTTGTCACCTATGATTTCATCCAGACCTATAAAGTCCTCCTCCTGTCATGTGGCATCTCCCT GGAGCCCAGTTATGAAGACCCCAAGGTGGCATGCTGGCTTCTCGACCCAGATTCTAAGGAGCCAACTCTT CATAGCATAGTGACCAGTTTTCTCCCCCATGAGCTAGCACTCCTAGAAGGAATGGAGACAGGCCCAGGAA TTCAGAGCCTGGGGCTAAATGTGAACACTGAACATTCTGGGAGGTACAGAGCATCTGTGGAGTCTGTTCT CATCTTCAACTCCATGAATCAACTCAACTCTTTGTTGCAGAAGGAAAACCTTCATGATATTTTCTGCAAA GTGGAGATGCCCTCTCAGTACTGCCTGGCCTTGCTAGAACTAAATGGAATTGGCTTTAGTACTGCGGAGT GTGAAAGCCAGAAGCATGTTATGCAAGCCAAGCTGGACGCCATTGAGACCCAAGCCTATCAGCTGGCTGG CCACAGTTTTTCCTTTACAAGTGCAGATGACATCGCACAGGTTTTATTTTTGGAACTAAAGTTACCACCG AATGGAGAGATGAAAACGCAAGGCAGCAAAAAGACTCTGGGTTCCACCAGAAGAGGGAATGAGAGTGGCC GCAGAATGAGGCTGGGAAGACAGTTCAGCACTAGTAAGGATATTTTAAATAAATTAAAGGGCTTGCATCC TTTACCAGGCCTGATACTGGAATGGAGAAGAATCAGTAATGCTATTACCAAGGTGGTCTTCCCTCTGCAG CGGGAAAAGCACCTGAACCCCCTTCTCAGAATGGAGAGGATCTACCCGGTGTCACAGTCACACACGGCTA CAGGACGGATAACCTTTACAGAACCCAATATCCAGAATGTGCCAAGAGATTTTGAGATTAAAATGCCAAC ACTAGTAAGGGAGAGCCCACCTTCTCAAGCTCCAAAAGGCCGGTTCCCAATGGCAATAGGACAAGATAAG AAGGTTTATGGCCTGCACCCCGGGCACAGAACACAGATGGAAGAGAAGGCCTCAGATAGAGGGGTGCCAT TTTCAGTGAGCATGCGCCATGCCTTTGTGCCTTTCCCAGGTGGGCTGATCCTGGCTGCCGACTACTCTCA GCTTGAACTGAGGATCCTGGCTCACTTGTCTCGTGACTGTCGTCTCATTCAAGTCTTAAATACGGGAGCG GATGTGTTCAGGAGCATTGCTGCCGAGTGGAAGATGATTGAGCCTGATGCTGTTGGTGACGATCTGAGGC AGCACGCAAAGCAGATTTGCTATGGAATTATATATGGAATGGGAGCTAAATCTTTAGGAGAGCAGATGGG CATTAAAGAAAATGATGCTGCTTCCTACATTGACTCTTTCAAGTCCAGATATAAAGGGATTAATCATTTC ATGAGGGACACAGTGAAGAACTGTAGAAAAAATGGATTTGTTGAGACAATTTTGGGAAGACGTAGATATT TGCCAGGAATCAAAGACGACAATCCTTATCATAAAGCACACGCTGAACGTCAGGCCATCAACACAACAGT CCAGGGATCAGCTGCTGATATAGTCAAAATAGCCACAGTTAATATTCAGAAGCAATTAGAGACCTTCCGT TCAACCTTCAAATCCCACGGTCATCGGGAAAGTATGCTCCAGAATGACCGAACAGGATTGTTGCCCAAGA GGAAACTTAAAGGGATGTTTTGTCCCATGAGAGGAGGATTCTTCATCCTTCAACTTCACGACGAGCTTTT ATATGAAGTGGCAGAAGAGGATGTTGTTCAGGTGGCTCAGATTGTCAAGAATGAAATGGAATGTGCTATA AAACTTTCTGTGAAGCTGAAAGTGAAAGTGAAAATAGGTGCCAGCTGGGGAGAGCTGAAGGACTTTGATG TGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_029977
Insert Size	7635 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029977.2, NP_084253.1
RefSeq Size	8569 bp
RefSeq ORF	7635 bp
Locus ID	77782
UniProt ID	Q8CGS6
Gene Summary	DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25275444, PubMed:25642963). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (PubMed:25642963). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates. Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (By similarity). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (PubMed:16222339, PubMed:16172387, PubMed:16890500, PubMed:17449470). However, POLQ does not play a major role in somatic hypermutation (PubMed:18485835).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.