Piezo1 (NM_001037298) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9630020g22; Fam38a; mKIAA0233
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225355 representing NM_001037298 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCACGTGCTGGGCGCCGGGCTCTACTGGCTGTTGCTGCCCTGCACGCTCCTGGCGGCCTCCC TGTTACGCTTCAATGCTCTCTCGCTGGTCTATTTGCTGTTTCTACTGCTGCTGCCCTGGCTTCCAGGCCC CTCAAGACACAGCATACCAGGTCACACAGGTCGCCTGCTCCGTGCACTACTCTGCCTCAGCCTCCTCTTC CTGGTGGCCCACCTTGCCTTTCAGATATGCCTACACACCGTGCCTCACCTGGACCAGTTTCTGGGACAAA ACGGTAGCCTTTGGGTGAAGGTGTCTCAACACATAGGGGTTACGAGGCTGGACCTGAAGGACATCTTTAA CACCACCAGGCTGGTAGCACCTGACCTGGGAGTGCTGCTGGCGTCCTCCCTTTGCCTTGGCCTCTGTGGA CGCCTCACGAGGAAAGCCCGGCAGAGTCGGCGCACCCAGGAGCTGGATGATGACGATGACGATGATGACG ACGATGAAGACATAGATGCTGCCCCAGCCGTGGGGCTGAAGGGAGCCCCTGCCCTGGCAACCAAACGCAG GCTGTGGCTGGCCTCCCGCTTCCGGGTCACGGCCCACTGGCTGCTGATGACCTCTGGACGGACGCTGGTC ATTGTGTTGCTGGCCCTGGCAGGCATAGCCCACCCTTCGGCCTTCTCCAGCGTCTACCTGGTGGTGTTCC TGGCCATCTGCACCTGGTGGTCCTGTCACTTTCCTCTCAGCCCCCTGGGCTTTAACACCCTCTGTGTCAT GGTGAGCTGTTTTGGTGCCGGCCATCTCATTTGCCTATACTGCTATCAGACACCATTTATCCAGGACATG TTACCCCCTGGGAACATCTGGGCCAGGCTATTTGGTCTCAAGAACTTCGTAGACCTCCCTAACTACTCCA GCCCCAACGCCCTGGTACTCAACACTAAGCACGCCTGGCCCATCTATGTGAGTCCCGGAATCCTGCTGCT GCTATATTACACAGCCACCTCTCTCCTGAAGCTCCACAAGAGCTGTCCCTCAGAGCTGAGGAAGGAGACA CCCAGGGAGGATGAGGAGCATGAGCTGGAACTGGACCACCTGGAGCCAGAGCCCCAGGCTAGGGACGCCA CCCAGGGTGAGATGCCCATGACCACGGAACCTGACCTTGACAACTGCACCGTGCATGTACTAACCAGCCA GAGCCCTGTCCGCCAGCGTCCAGTTCGCCCCAGGCTGGCTGAGCTGAAAGAGATGTCACCGCTACATGGC CTGGGCCACCTCATCATGGACCAGAGCTATGTGTGTGCCCTGATTGCCATGATGGTGTGGAGCATCATGT ACCACAGCTGGCTGACCTTCGTCCTGCTGCTCTGGGCCTGCCTCATCTGGACGGTGCGTAGCCGCCACCA GCTGGCTATGCTCTGCTCGCCCTGCATCCTGCTGTATGGGCTGACGCTCTGCTGCCTGAGGTACGTGTGG GCCATGGAACTTCCTGAGCTGCCCACCACCCTGGGCCCTGTCAGCCTGCACCAGTTGGGACTGGAACACA CACGCTACCCTTGCCTGGACCTCGGTGCCATGCTCCTCTATCTGCTCACATTCTGGCTCCTTCTGCGTCA GTTCGTGAAGGAGAAGCTGCTGAAGAAGCAAAAGGTGCCTGCGGCGCTTTTGGAGGTCACGGTGGCCGAC ACTGAGCCCACACAGACCCAGACGCTGCTGCGGAGCCTGGGGGAGCTGGTCACCGGCATCTACGTCAAAT ACTGGATCTATGTGTGCGCCGGCATGTTCATTGTGGTCAGCTTCGCCGGCCGCCTGGTGGTCTACAAAAT CGTCTACATGTTCCTCTTCCTGCTGTGCCTCACCTTGTTCCAGGTCTACTACACCCTGTGGAGGAAGCTG CTGCGTGTCTTCTGGTGGCTCGTGGTGGCCTATACAATGCTCGTGCTCATCGCTGTGTACACCTTCCAGT TCCAGGACTTCCCCACCTATTGGCGCAACCTCACGGGCTTCACGGACGAGCAGTTGGGCGACCTGGGCCT GGAGCAGTTCAGTGTGTCGGAGCTCTTTTCCAGTATCCTCATCCCTGGCTTCTTCCTGCTGGCCTGCATC CTGCAGCTGCACTACTTCCACAGACCGTTCATGCAGCTCACTGACCTGGAGCACGTGCCGCCACCAGGCA CCCGCCACCCTCGATGGGCTCACAGGCAGGATGCAGTGAGCGAGGCCCCTCTGCTTGAGCATCAGGAGGA AGAGGAAGTCTTCAGGGAAGATGGGCAGAGCATGGATGGGCCCCACCAGGCCACACAGGTCCCTGAGGGT ACGGCCAGCAAGTGGGGCCTGGTGGCTGACCGGCTGCTGGACCTAGCGGCCAGCTTCTCGGCTGTCCTCA CCCGAATCCAGGTGTTCGTTCGGCGCTTGCTAGAACTTCACGTCTTCAAGCTGGTGGCCCTCTACACTGT CTGGGTGGCCCTGAAAGAAGTGTCTGTGATGAACCTGCTGCTGGTGGTGCTATGGGCCTTCGCCTTGCCC TATCCGCGCTTCCGGCCCATGGCTTCCTGCCTGTCCACCGTGTGGACCTGTATCATCATTGTATGCAAGA TGCTCTATCAGCTCAAGATTGTCAACCCGCATGAGTACTCCAGCAACTGCACTGAGCCCTTCCCCAACAA TACCAACTTGCAGCCTTTGGAGATCAACCAGTCTTTGCTGTACCGTGGCCCTGTTGACCCTGCCAACTGG TTTGGGGTGCGGAAGGGTTACCCCAACTTGGGCTATATCCAGAACCACCTGCAGATCCTTCTGTTGCTGG TGTTTGAGGCCGTGGTGTACCGGCGCCAAGAGCACTACCGCCGGCAGCACCAGCAGGCCCCTCTGCCCGC CCAGGCTGTGTGCGCAGATGGCACCCGCCAGAGGTTGGACCAGGACCTACTTAGCTGCCTCAAGTATTTC ATCAACTTCTTCTTCTACAAATTCGGGCTGGAGATCTGCTTCTTGATGGCCGTGAATGTGATTGGGCAGC GTATGAACTTCATGGTGATCTTACACGGTTGCTGGTTGGTGGCCATCCTTACACGCCGGCGCCGTGAGGC CATCGCCCGCCTCTGGCCTAACTACTGTCTGTTCCTCACGCTGTTCCTGCTGTACCAGTACCTGCTGTGT TTGGGCATGCCCCCTGCACTGTGCATTGACTATCCATGGCGCTGGAGCAAGGCCATCCCCATGAATTCCG CCCTCATCAAGTGGCTGTACCTACCTGACTTCTTCAGAGCCCCCAACTCCACCAACCTTATCAGTGACTT CCTCCTGCTGCTTTGCGCCTCCCAGCAGTGGCAGGTCTTCTCAGCGGAGCGAACGGAGGAGTGGCAACGC ATGGCGGGCATCAACACTGACCACCTGGAGCCCCTGCGTGGGGAGCCCAACCCTATACCCAACTTCATCC ACTGCAGGTCCTATCTGGATATGCTGAAGGTGGCCGTCTTCCGCTACCTGTTCTGGCTGGTGCTCGTTGT GGTGTTTGTTGCGGGGGCCACCCGCATAAGCATCTTCGGGCTGGGGTACCTGCTAGCCTGCTTCTACCTG CTGCTGTTTGGCACTACCCTGCTGCAGAAGGACACGCGAGCCCAGCTCGTGCTGTGGGACTGCCTCATCC TCTATAATGTCACTGTCATCATCTCTAAGAATATGCTGTCGCTCCTGTCCTGTGTCTTCGTGGAGCAAAT GCAGAGCAACTTCTGCTGGGTCATCCAGCTCTTCAGCCTCGTGTGCACAGTCAAAGGCTACTATGATCCC AAAGAGATGATGACCAGGGACCGGGACTGCCTGCTGCCTGTGGAGGAGGCCGGGATCATCTGGGACAGTA TCTGCTTCTTCTTCCTGCTCTTGCAACGGCGCATCTTTCTCAGCCACTACTTCCTGCATGTCAGCGCTGA CCTGAAAGCCACAGCCCTGCAGGCATCCAGGGGCTTTGCCCTCTACAATGCAGCCAACCTGAAGAGCATC AACTTCCATCGCCAGATTGAGGAGAAGTCCCTGGCCCAGCTGAAAAGACAGATGAAGCGCATCCGTGCCA AACAGGAGAAGTACAGGCAGAGCCAGGCAAGTCGTGGCCAACTCCAGTCCAAAGACCCTCAGGATCCCAG CCAGGAGCCAGGGCCTGACAGCCCAGGGGGCTCCTCCCCGCCACGGAGACAGTGGTGGCGCCCCTGGCTG GACCACGCCACAGTCATCCACTCTGGCGACTACTTCCTGTTTGAGTCAGATAGCGAGGAGGAAGAGGAGG CCCTACCTGAGGACCCCAGGCCTGCAGCTCAGAGTGCCTTCCAGATGGCATACCAGGCATGGGTAACCAA TGCCCAGACAGTGCTGAGGCAGCGTCGGGAGCGGGCACGGCAGGAGCGGGCAGAGCAGCTGGCTTCTGGA GGTGACTTGAACCCAGATGTGGAACCAGTAGATGTCCCAGAAGATGAGATGGCAGGCCGTAGCCACATGA TGCAGCGTGTGCTAAGCACCATGCAGTTCCTGTGGGTGCTGGGCCAGGCCACGGTAGACGGGCTGACGCG CTGGCTGCGTGCATTCACGAAGCACCACCGCACCATGAGCGATGTGCTGTGCGCAGAGCGCTACCTGCTC ACCCAGGAGCTTCTTCGGGTTGGAGAGGTACGCCGAGGTGTGCTGGACCAGCTTTATGTGGGTGAAGATG AGGCCACATTGTCAGGTCCCATGGAGACCCGGGATGGACCCAGCACAGCCTCAAGTGGGCTGGGAGCCGA AGAGCCTTTGAGTAGCATGACAGACGACACCAGCAGCCCCCTGAGCACAGGCTATAACACCCGCAGTGGC AGTGAGGAGATTGTCACCGACGCTGGGGACCTCCAGGCTGGGACCTCCCTGCACGGCTCCCAAGAGCTTT TAGCCAATGCTCGTACCCGGATGCGCACGGCCAGCGAGCTGCTACTGGATAGGCGCCTGCATATCCCTGA GCTGGAGGAGGCCGAGCGGTTTGAGGCACAGCAGGGCCGGACTCTGCGGCTGCTCAGGGCTGGGTACCAG TGCGTGGCGGCACACTCGGAGCTGCTCTGTTACTTCATCATCATCCTTAACCACATGGTGACAGCCTCGG CTGCCTCCCTGGTGCTGCCCGTGCTTGTGTTCCTGTGGGCCATGCTGACCATCCCGAGGCCTAGCAAGCG CTTTTGGATGACAGCTATCGTCTTCACTGAGGTCATGGTGGTCACCAAATACCTGTTCCAGTTCGGCTTC TTCCCCTGGAACAGCTACGTTGTGCTGCGGCGCTATGAGAACAAGCCCTACTTCCCTCCGCGAATCCTGG GCCTTGAGAAAACGGACAGCTACATCAAGTATGACCTGGTGCAGCTCATGGCCCTCTTCTTCCACCGCTC GCAGCTACTGTGTTATGGCCTCTGGGACCATGAGGAGGATCGCTATCCCAAGGACCATTGCAGGAGTAGT GTGAAGGACCGGGAGGCCAAGGAAGAGCCAGAAGCTAAGCTGGAATCGCAGTCTGAGACAGGCACTGGGC ATCCCAAGGAGCCAGTGTTGGCCGGTACTCCCAGGGACCACATCCAAGGGAAAGGAAGTATTAGATCCAA GGATGTTATCCAAGATCCCCCAGAGGACCTTAAGCCCCGGCACACGAGGCACATCAGCATACGCTTCAGG AGGCGCAAGGAGACTCCAGGACCCAAAGGAACAGCAGTCATGGAGACTGAGCACGAGGAGGGAGAAGGAA AAGAAACTACAGAGAGAAAGAGGCCGCGTCACACTCAAGAAAAATCGAAGTTTCGGGAGAGAATGAAGGC AGCTGGGCGCCGGCTGCAGAGCTTCTGTGTGTCACTGGCCCAGAGCTTCTACCAACCCTTGCAGCGTTTC TTCCATGACATTCTGCACACAAAGTACCGGGCGGCCACCGACGTCTACGCCCTCATGTTCCTGGCCGATA TTGTCGACATCATCATCATCATCTTTGGTTTTTGGGCTTTTGGGAAGCACTCTGCAGCCACAGACATTGC ATCCTCGCTGTCAGATGACCAGGTGCCACAGGCCTTCCTGTTCATGCTGCTGGTCCAGTTTGGCACCATG GTCATCGACCGTGCCCTCTACCTGCGCAAGACTGTCCTGGGAAAGCTGGCCTTTCAGGTGGTCCTGGTGG TGGCGATTCACATCTGGATGTTCTTTATCTTACCGGCTGTCACTGAGAGGATGTTCAGCCAGAATGCGGT GGCACAGCTGTGGTACTTCGTCAAGTGCATTTACTTTGCCCTGTCCGCCTACCAGATCCGCTGTGGCTAC CCCACCCGTATCTTGGGCAACTTCCTCACCAAGAAATACAACCATCTAAACCTCTTCCTTTTCCAGGGGT TCCGTCTAGTGCCGTTCCTGGTGGAGCTGCGGGCCGTCATGGACTGGGTGTGGACCGACACCACGCTGTC CCTGTCCAACTGGATGTGTGTGGAAGACATCTATGCCAACATCTTTATCATCAAGTGCAGCCGAGAGACA GAGAAGAAATACCCCCAGCCCAAGGGGCAGAAGAAGAAGAAAATTGTCAAGTATGGTATGGGAGGCCTCA TTATCCTCTTCCTCATCGCCATCATCTGGTTCCCTCTGCTCTTCATGTCACTGATCCGCTCTGTGGTCGG GGTCGTCAACCAGCCCATTGATGTCACCGTCACCCTCAAGCTAGGCGGCTATGAGCCACTGTTCACCATG AGCGCCCAGCAGCCATCCATTGTGCCATTCACACCCCAGGCCTACGAGGAGCTGTCCCAGCAGTTTGACC CCTATCCACTAGCCATGCAGTTCATTAGCCAGTACAGTCCTGAGGACATCGTCACTGCACAGATCGAGGG CAGCTCGGGGGCGCTGTGGCGCATCAGCCCACCCAGCCGAGCCCAGATGAAGCAGGAGCTGTACAACGGC ACAGCCGACATTACACTGCGCTTTACCTGGAATTTCCAAAGGGACCTGGCCAAGGGTGGCACTGTGGAGT ATACTAATGAGAAGCACACCTTGGAGCTGGCCCCCAACAGTACGGCACGAAGGCAGCTGGCCCAACTGCT CGAGGGCAGACCTGACCAGTCAGTGGTCATTCCCCATCTCTTCCCCAAGTACATTCGTGCTCCCAATGGG CCTGAAGCCAACCCTGTGAAGCAGCTGCAGCCAGATGAGGAAGAGGACTACCTTGGTGTGCGCATCCAGC TGCGGAGGGAGCAAGTGGGCACAGGGGCCTCTGGGGAGCAAGCGGGCACCAAGGCCTCCGACTTCCTCGA GTGGTGGGTCATCGAGCTGCAGGACTGCAAGGCTGACTGCAACCTGCTGCCCATGGTCATCTTCAGTGAC AAGGTCAGCCCACCTAGCCTGGGCTTCCTGGCCGGCTACGGGATTGTGGGGCTGTACGTCTCCATCGTGC TGGTGGTTGGCAAGTTTGTGCGGGGCTTCTTCAGCGAGATCTCTCACTCCATCATGTTCGAGGAACTGCC GTGTGTGGACCGCATCCTCAAGCTGTGCCAGGACATCTTCTTGGTGCGCGAGACCCGGGAGCTGGAGCTG GAGGAGGAGCTATACGCCAAGCTCATCTTCCTGTACCGATCTCCAGAGACCATGATTAAGTGGACACGTG AGAGGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001037298
Insert Size	7641 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001037298.1, NP_001032375.1
RefSeq Size	8216 bp
RefSeq ORF	7641 bp
Locus ID	234839

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