Epg5 (NM_001195633) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4732475F16; 5430411K18Rik; AI661957; AW456499; mKIAA1632
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225362 representing NM_001195633 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCTGTGAAGCCCCGCCGGGCCAAGGCCAAGGCCAGCCGCACCAAAGGCAAGGAAAAGAAGA AGCATGAAGCCCTTCAGACTTGTGATGCAGGTCCCCTGCCAGAGACCTGTCGCGAGCAGGAGAGCCCGTG TCCAGCTTCTGAACTCAAAGGAGATGACCTGAAGTCGTCTGCTGACCCCCAGCTCCACAGTGATGTGTGT GGATGGAATGAAAGTGAGATGTTTGATATACCACTCACCTCCTTGACTATAGGGGATGAAGGTCCCCCGG TACAGGACACAGAGGACCTAAAAGAAAGGGGAGAGGTCACAGCCGGTGATGGAGATGATGAAATGGAGTT GAAGGTGGACCCTGGGGACAACGTTATAGCTAAAGGTGAACCTTGTAAGAACTTCCCGGAAGTAGAGGAC CACACACTCATCCAGTGTGGGCCTCCAGAGAGCACACTGCAGCCTGACTTTCCCTGCACTCAGCAGGCAG TGGAGGGCTCACATGCCAGAGAACATCCGACTAGGAAGCAAGATGAAGCCGCCCTCGGCTGTTCAAAGGT GTTTCAGAATGTTAGCTTGCACAGTTCTTATGAAGCCAAAGAAGTTTCTCAGCCACCGAGAGTGAAGAAA CTATATCCTGAGTTGCCAGCTGAAATTGCTGAAGTACCAGCTCTGGTGGCAGTGAAGCCATTGCTCCGTA GTGAGCGACTCTACCCAGAACTCCCGTCTCAACCAGAAGTGACACCCTTCACTAAAGAACAGCTCAAACT CTTGGAGCCTGGCTCATGGCTGGAAAATGTTGCATCATATGTAGAAGAATTCGATAACATTGCTCATCAG GACAGACACGAGTTTTATGAGCTGCTTTTGAATTACTCACGGTGCAGGAAGCAGTTGCTGTTGGCTGAAG CTGAGCTGCTCACTCTGATGTCTGACTGCCACAGTGCCAAAAGTCGACTGTGGCACTTTAAGGATGAGCA GATGGCCGTACAGGGCATCTGTGCAGATCAAGTGAAAGTCTACGGGCATCACCACTACCAGCGAGTAGAG ATGAATGAAAATGTCCTGGGCGAGCTGAAGAAGCTGTTTGATGCCAAATCTGAGCACCTCCACCAGACCC TGACCCTCCACTCGTACACTTCTGTGCTCTCGAGGTTGCAAGTGGAGTCCTACATCTTCACACTGCTCAA TAGCTCAGCTGCTCTGAGATCTTTAGCAGTTTATCAGGCAGACCAAGTTCCTAAACTGACAGAAAGCATC CCCTCTGATGTGTGTCAGCTGAAGGAGTGCATTAGTGTCTTGTTCATGTTCACCAGGAGAGTGAGTGAGG ATGCCCAGTTCCATGAGGACATCCTGCTCTGGCTGCAGAAGCTGGTGTCCGTGTTACAACGAGTCGGCTG TCCTGGGGATCACTTCTTTCTCCTGAACCACGTTCTCCGCTGCCCAGCTGGCATTCGTAAATGGGCTGTT CCTTTTATACAGATCAAAGTGTTGAATAACCCTTCAGGGGTCTTTCATTTTATGCAGTCCCTTGCCCTGC TGATGTCTCCTGTCAAAAACCGAGCGGAGTTTATGTGCCACATGAAGCCCAGTGAGTGGAAGCCATCATC TTCAGGGCCAGCATCTGGGAACTGGACACTTGTAGATGAAGCGGGGGAAGAGGATGAAGACCCTGAGACC AGTTGGATTCTCCTTAATGAAGACGACTTGGTCACCCTTTTGTCCCAGTTTCCCTTTCAGGAGCTCTTTC AGCATCTTCTTGGGTTTAAAGCCAAAGGTGATTATTTACCTGAAACAACAAGGCCCCAAGAGATGATGAA AATTTTTGCCTTTGCCAACTCATTAGTGGAGCTTCTGGCTGTGGGACTAGACACCTTTAACAGAGCCCGC TACAGGCAGTTTGTGAAGCGCATTGGATACCTGATCAGGATGACCCTTGGCTATGTCAGTGACCACTGGG CTCAGTATGTGAGCCACAGCACAGGCGCGGGGCTAACACCGCAGCCCTACTCCATGGAGAAACTGCAGGT GGAATTTGATGAGCTGTTTCTCAGGGCTGTCCTCCATGTCCTGAAAGCCAAAAGACTTGGCATTTGGCTG TTCATGTCTGAGATGCCCTTCGGCACGCTGTCGGTCCAGATGCTCTGGAAGCTCTTGTACCTCATGCACC AGGTGGAGAGTGGGGACCTGCAGCAACTCTGTGCCTCCCTGCAGCCGGCCGAGTGCAAGAGGCGACTCCA AGACCCAGAACATTTTGCGAGCTTTGAGAAGTGTCTTTCTTCCATCAACAGCTCAGAGGAGATCTGTCTC CTCACGGCCTTTGCTCAGATGGCTCGAGCCAGAAGAACCAATGTGGATGAAGACTTCATAAAAATCATTG TCCTGGAGATCTATGAGGTATCTTATGTCACACTGTCTACCAGAGAGACTTTCTCAAAGGTTGGTCGAGA GCTTCTGGGAGCCATTGCAGCTGTTCACCCTGAGATTATCTCTGTCCTTTTGGACAGAGTGCAGGAGACC ATTGACCAAGTTGGAATGGTTTCTTTATACTTGTTTAAAGAACTGCCTTTGTATCTTTGGCGACCTTCTG CCCCAGAGATAGCTGTGATTCGGGACTGGTTATTGAATAATAATCTGACAGCAGTGAAGAACAAGTTGGC CTGTGTGATCCTAGAAGGACTGAATTGGGGATTTACTGAACAGGGTACCCTTCATTTGGATCAAGCATTG CACACGGAAGTGGCCTTACTGGTTCTCGAGGCTTATCAGAAATACCTGGCACAGAAACCTTATACTGGGC TTATTTCTGAAAGTATGAAACAGGTTTCCTATTTGGCCAGTATTGTTCGATATGGAGAGACTCCTGAGAC CTCATTTAACCAGTGGGCCTGGAATTTAATCTTGAGGTTAAAATTGCACAAAAATGATTTTGGAAGACAG AACTTTCCAGTCATTCCCTTCTGCAGCACAGTACCTGACATGACAGAATCGTCCATGTTTCACCCTCTCT TGAAGGCTGTGAAATCAGGCTTGCCCATTGGCTGCTATCTCGCTTTAGCTGTGACAGCTGTTGGCCACAG CCTTGAGAAGTTCTGTGCAGAAGGCATCCCACTGCTGGGCGTGCTGGTCCAGTCACGGCATCTGAGAGCA GTGGTCCATGCCCTAGATAAGATCCTGCCTGTGTTCTACCCTTACCAGTGCTACCTTCTGAAGAACGAGC AGTTTTTGTCGAACCTTCTCCTCTTCCTACAATTGGACAGCGGTGTTCCCCAGGGGGTCACACAGCAGGT CACCCACAGGGTGGCCCAGCATCTGACAGGAGCCGTCCATGGAGACAATGTGAAACTTCTCAGCAGCATG ATCCAGGCACACATATGTGTAAGCACTCAGCCGGATGGAGTGGGTCCTGTGGCTGTGTTGGAGTTCTGGG TCCAGGCTCTCATAAGCCAGCATCTGTGGTACCGAGAACAGCCCATTCTCTTCCTCATGGACCACTTGTG TAAGACAGCGTTTCACCTGATGCAGGAAGATTGTGTGCAAAAATTGCTCTACCAACAGCACAAGAATGCT TTGGGTTACCACTGTGACCGGAGTCTGCTCTCTTCCTTGGTGAACTGGATTGTGGCAGGGAACATCACGC CTTCCTTCGTGGAGGGCTTGTCCACGTCCACACAGGTTTGGTTTGCATGGACTGTGTTGAACATGGAATC CATCTTTGAAGAGGACTCTCAGCTCCGAAGAGTTGTTGAAAGGGAATTGGTTATAAATGCGTTTAGCCCT GACCAAGCTCTAAAGAAAGCCCAGGTCCAGCTGAAGCTGCCCATCGTCCCGTCCCTGCAGCGGCTGTTGA TTTATCGCTGGGCCCACCAGGCTCTGGTCACACCTTCTGATCATCCCCTTTTGCCACTCATTTGGCAGAA GTTCTTCCTCCTGTATCTTCATCGCCCAGGACCACAATATGGGTTACCTGTGGATGGTTGTATTGGAAGA AGGTTTTTTCAAAGTCCCTCGCACGTTAATTTGTTGAAAGACATGAAGAGACGCCTGACGGAGGTGGCTG ACTTCCACTATGCTGCCAGCAAGGCCCTCCGTGTTCCAGCAGAGGGCAGTGAAGGGACACCAGAAGGGCA GGCTGGCACCCCTGGCTTCCTGACTTCCCCAGAACTGCACAGGGAACTAGTGAGGCTCTTTAATGTATAT GTATTATGGCTAGAAGATGAAAATTTTCAGAAAGGAGATACCTATATTCCTTCTTTACCAAAGCACTACG ATGTTCACAGGCTAGCAAAAGTGATGCAGAATCAGCAGGACCTGTGGATGGAGTATGTGAATATGGAGCG CATACAGCACGAGTTCCAGGAGACAGTTGCTCTGTGGACACAGGCCAAGCTTGAGTCCCATGCCGCACCC TGCAGCTCTTCAGCACAGCTGGATTTCACGGACCCCTTGCTGGCTAAAGCAAGGGTTCTGAGTAACTTAG AGAAGCACGAGGCTCCACATCCTCCTCTTCTTCTGCACCCCGTGAGGCCTCCCGTGCCACTCATTCCCTC TGCTGCGCTGTTGACACAGAAGGACAGCACCCAGCTCATGTGCACAGACCTGAATCTGCTGCAGCAGCAG GCCAGGAGTGCCACTCTGCGGGAGTCGCAGCAGGTTGCCTTGGATGGCGAGCTGTTGGAGACCATGCCCA AACAGTACGTTAACCGGGAAGATCAGGCCACCCTGCATCTGGAGTGCAGAGGCAGCAGTGGCAAGAAGTG CCAAGGAGCAGCAGTTGTAACGGTCCAGTTTGAAGGCATGAACAAGAATGAAGCCGTAAGCCAGCAGATC CATGTTCTGCAGAAAGAAGTGAGGCAGCTCCAAGCGGAAGCTGCTCAGCCCCCGGCACTGAATGTTGTGG AGGCCGCTGTGCATGCAGAAAACCTGATCACGGCCTTAGTGAACACTTATAAGTTACAGCCTACACCTGG AGTTCAGAAACTTGGCATTAGCCTTTTCTTTACTGTTGTGGACCATGTCAGCGATGAGACCCAGCGCCAT CCTCCTACAAGGCAGTTCTTCACTTCATGCATCGAGATCTTGGGACAGGTGTTTGTTAGCGGCACTAAGT CCGAGTGCAGGAAGCTGCTCCAGACCATCCTGAAGAACAGGCGGCTCTGCTCGCTGCTGGCTCCTTTCTT CACGCCCAATGCTGCGCCCGCCGAGTTCATCCAGCTGTATGAGAGAGTGGTGACTTGTCTGCGCGAGGAC AACAGCGACGTGATCTTCATGCTGCTCACCAAGTTTGATATTCAGCAGTGGTTAAACAGCACTAAGCCCC CTCTGTCTGACCGCACCAGGCTCCTGGAGTCCATTCACTTGGCACTCACTGCCTGGGGCCTTGAACCAGA GGAAGATATTCTGATGCCATTTAATCTTTTCTGCAAGCACTGGACTCACCTCCTACTCTACCAGTTCCCT GACCAGTACAGTGATGTCCTCAGGCTGCTGGTGCAGAGCTCTGCCGAGCAGCTGCTGAGTCCCGAGTGCT GGAAAGCCACTTTGAGAGCCCTGGGCTGTTATGCCCCAAGCAGCCAGCAGGGGGCGGCGTCTGTGGAGAG CTCTGGGCTTCACAGTGCCTCCCGTGTTCTCCTGTCAGACAAGCAGGTAATGGAGACGGTACAGTGGCTT TCTGACTTCTTTTATAAGTTGCGGCTCTCCAAGTTGGACTTTAAAAGCTTTGGATTATTCTCAAAGTGGA GTCCTTACATGGCTGATGTGAAGACATTTCTGGGATACCTTGTGAAAAGGCTGACTGACTTAGAAATAGC CTCCTTGTCTCAGGACCCAACCGCCAGCAGCAAAGAAGTGCTGAGGTCCCTGCACGCGCAGATCATCCAG CTCTTTAAGCCCTGGATCCTGGTTTTAGAGGACGCGGAAAGTAGCCACCAGCGACATTACCCGTGGCTGG AGAGTGACACTGCGGTGGCCTCCAGCATCGTGCAGCTCTTCTCTGACTGTGTCGGCTCATTGCACACGAG TTTTAAAGACAGGCTGTTGCCAGGCGATGAGGGAGCCCTGCGGTTGCACCTGCTGCACTACTGTGAAACA TGCACAGCACCCAAGATGCCGGAGTTCATTCTCTATGCCTTCCACAGTGCATACCAGAGACTGGAGTGGA AGGACTTGCACCCTGACCAGAGGCTCATGGAGGCCTTCTTTAAGGTGGAACGTGGAAGCCCCAAGAGTTG TTTCTTGTTTTTGGGGTCTGTACTTTGCAGAGTCAACTGGGTTAGTGTGCTGTCTGACGCCTGGAATCCC AGCCCCCTTCCGGAAACCCAGAGCATGGCTGTCTGCCTCCTTTTCATGATGGTTTTACTAGCAAAGGAGG CTCAGCTCGTTGACGAGCCAGATTCACCTTTACTGAGTCTCCTTGGACAGACAAGCTCACTCTCATGGCA TCTTGTGGACCTTGTGTCATACCAGAGTGTGTTGGGTTATTTCAGCAGTCATTACCCACCATCCGTCGTC CTGGCAAATGACTGTTCCTCGGAACTGATAGTGAAGCTGTTAAAAGTGTCTGCAGGGCTTTCTGCTCATG CTGACGGCCGGAAGCACGTGGACATAGTTCCAAAATGCCAGGCTTTCACTCATCAGATGGTTCAGTTTCT CAGTGCCTTGGAACAGACTGGAAAAATCACCTTCCCAGCACTGGAGCGGGAAATATCTAAGCTGTTGGAT GACATCATTATCTTTAACCCACCCGACATGGACAGCCAGACCCGCCACATGGCCCTCAGCAGCTTCTTTG TGGAAGTCCTGATGATGATGAACAATGCGGCTGTTCCAACAGCAGAGTTCCTTGCTGTCAGTATTCGGAC CTGGATTGGCCAAAGAGTGCATGGCTTGATAGTGCTGCCCCTCTTAACAGCAGCCTGCCAAAGCCTGGCG TCTGTCCGCCACATGGCGGAGATCACCGAAGCCTGCATCATGGCCTACTTCAAGGAAAGCTCCCTCGATC AGAACTTAGGATGGGGCCCCGTTCTGGTGTCCCTTCAAGTTCCCCAGCTCACCGCGCGAGACTTTCTGGA GGAGTGCTTAGCCCTCGGTAGTTGTTTGACTCTCTATGTCTACCTGCTTCAGTGTCTGAACAGTGAGCAG ACGGTGAAGAACGACATGAAAATGCTCCTCGTCGTCAGCGGCTGGCTGGAGCAGGTGTACCCGAGCTCTG CACAGGAAGAGGCAAAGCTGTTTCTGTGGTGGCATCAAATCCTGCAGCTGTCTCTGATTCAGTTGGAGCA GAACGACTCCGTCCTGACAGAGTCCGTCATCCGGATCCTGCTCATGCTGCAGAGCAGGCAGAGCCTTATG GCCGAGGAGAGGCTCAGCTCTGGGATTCTGGGGGCAATCGGGTTGGGCCGGAGATCACCCTTGTCCAACA GGTTCCGAGTGGCTGCCCGAAGCATGGCTGCCTTCCTGTTGGTTCAGGTCCCTGCAGAAGACCAGATTCG TCTGAAACCCAGCTCTGAGTTACATCTGGCTCCAAAAGCTCAGCAGGTGCTGACAGCTCTGGAGTCCATG ACGCTCAGTAAGCAGTATGTGGAGTACCAGGACCAGATACTGCATGCTCTTCAGTTTATAAGGCATCCTG GCCACTGCCTTCAGAATGGGAAAAGCTTCCTGGCCCTTCTTGTCAACCGCCTCTATCCAGAAGTGCATTA CCTGGACAACATCCGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001195633
Insert Size	7719 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001195633.1, NP_001182562.1
RefSeq Size	9672 bp
RefSeq ORF	7719 bp
Locus ID	100502841
UniProt ID	Q80TA9
Gene Summary	Involved in autophagy. May play a role in a late step of autophagy, such as clearance of autophagosomal cargo. Plays a key role in innate and adaptive immune response triggered by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides from pathogens, and mediated by the nucleotide-sensing receptor TLR9. It is necessary for the translocation of CpG dinucleotides from early endosomes to late endosomes and lysosomes, where TLR9 is located.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.