Chd8 (NM_201637) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5830451P18Rik; AU015341; Chd-8; Du; Duplin; HELSNF1; mKIAA1564
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225364 representing NM_201637 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCCCATCATGGATCTTTTTGATGACCCAAACTTATTTGGCCTGGACTCTCTGACTGATGACA GCTTTAATCAGGTCACTCAAGACCCCATTGAAGAAGCACTTGGACTGCCAAGCTCTCTGGACTCCTTGGA TCAGATGAACCAAGATGGTGGAGGTGGTGATGTGGGGAATTCATCAGCAAGTGACCTGGTTCCTCCACCA GAGGAAACAGCTTCCACTGAACTTCCCAAAGAATCCACAGCTCCAGCTCCAGAATCCTTAACCTTGCATG ATTATACCACTCAGCCCACCAGCCAGGAGCAGCCAGCCCAGCCTGTCTTGCAGACATCAACACCAACAGC AGGACTCTTGCAGGTCTCCAAGAGCCAGGAGATCTTGAGCCAAGGGAATCCTTTCATGGGTGTCTCTGCC ACAGGTGTCTCCCCCAGTAATACTGGAGGACAACCATCTCAGTCAGCTCCTAAGATTGTTATTCTTAAAG CTCCACCAAACTCCTCCGTCACAGGCACCCATGTGGCACAAATTCAGGCCCAAGGTATCACCAGTACAGC TCAGCCCCTAGTGGCTGGGACAGCCAACGGTGGAAAAGTCACTTTTACCAAAGTGCTGACTGGTACACCC CTTCGACCAGGTGTATCCATTGTCTCTGGTAATACAGTGTTGGCCACGAAGGTCCCTGGGAACCAGGCTG CTGTTCAGCGCATTGTCCAGCCAAGCCGACCAGTAAAACAGCTGGTCCTCCAGCCAGTAAAGGGTTCAGC TCCTGCTGGGAATCCTGGGGCTGCCGGGCCCCCACTGAAGCCTGCAGTTACACTGACGTCTACACCTACC CAGGGTGAATCGAAACGCATCACTTTGGTCCTTCAACAGCCACAGTCCGGAGGTCCCCAAGGACATCGGC ATGTTGTGTTAGGGAGTCTACCAGGCAAGATAGTGTTACAGGGCAACCAGCTAGCAGCCTTGACTCAAGC CAAGAATGCCCAGGGGCAGCCTGCCAAAGTAGTAACTATTCAGCTGCAGGTGCAGCAGCCACAGCAGAAA ATCCAGATTGTACCTCAGCCTCCATCATCACAACCACAGCCACAGCCGCAGCCGCCACCCTCAGCCCAGC CGTTGACCCTGTCCTCTGTGCAGCAGGCTCAGATAATGGGGCCAGGCCAAAACCCAGGGCAGAGACTTTC TGTACCGCTCAAGATGGTGCTGCAGCCACAGGCTGGCTCTTCTCAGGGAGCCTCTTCTGGACTCTCTGTG GTTAAAGTTCTAAGTGCCAGTGAAGTGGCAGCCCTGTCATCCCCAGCAAGCTGTGCCCCTCATACTGCAG GGAAGACTGGGATGGAGGAGAACCGCAGGTTGGAGCACCAGAAAAAGCAAGAGAAAGCAAATCGGATTGT GGCAGAGGCCATTGCTAGGGCCCGGGCCAGGGGCGAGCAAAACATACCTCGAGTCCTGAATGAGGATGAG CTGCCTAGTGTCCGGCCAGAGGAGGAGGGTGAAAAGAAACGCAGGAAGAAGAGCAGTGGGGAAAGGCTGA AGGAGGAGAAGCCAAAGAAGAGCAAGACTGCTGCTGCCTCCAAAACGAAGGGCAAGAGTAAGCTAAACAC CATCACTCCTGTAGTGGGCAAGAAGAGAAAGCGTAACACATCGTCTGACAACTCTGACGTGGAAGTTATG CCTGCACAATCGCCCCGGGAGGACGAAGAGAGCAGCATCCAGAAGAGACGTTCAAACCGCCAAGTTAAGC GAAAAAAATATACAGAGGACCTGGATATAAAGATAACAGACGATGAAGAGGAGGAGGAGGTCGATGTAAC TGGTCCAATAAAACCCGAGCCCATCCTCCCAGAACCAGTGCAAGAGCCTGATGGCGAGACTCTTCCTTCC ATGCAGTTCTTTGTGGAGAATCCTAGTGAAGAAGATGCAGCTATTGTGGACAAAGTGCTTTCCATGCGAG TTGTGAAGAAAGAGCTTCCTTCTGGACAATACACTGAGGCTGAAGAATTCTTCGTCAAGTACAAGAACTA CTCTTATCTGCACTGTGAGTGGGCAACGATCTCCCAGCTGGAGAAGGATAAGAGGATCCACCAGAAACTA AAACGGTTCAAAACAAAAATGGCTCAGATGAGACACTTCTTTCATGAAGATGAAGAGCCTTTCAATCCAG ACTACGTAGAGGTGGATAGGATACTGGACGAGTCTCACAGTGTTGACAAGGATAATGGTGAGCCTGTAAT TTACTACCTGGTAAAATGGTGCTCTCTGCCCTATGAGGACAGTACGTGGGAGCTAAAAGAGGATGTTGAT GAGGGCAAGATTCGGGAATTTAAACGGATCCAGTCAAGGCACCCAGAACTGAGAAGGGTGAATCGTCCAC AGGCAAATGCCTGGAAGAAATTGGAGTTATCACATGAATATAAAAACAGAAACCAATTACGGGAATATCA GTTAGAAGGGGTCAACTGGCTCCTCTTTAATTGGTATAACAGGCAGAACTGCATCCTGGCTGATGAAATG GGATTGGGCAAAACTATTCAGTCAATCGCCTTCTTGCAGGAGGTATATAATGTAGGCATCCATGGTCCCT TTTTGGTCATTGCTCCATTGTCCACCATTACTAACTGGGAGCGTGAATTCAATACATGGACAGAGATGAA CACTATTGTGTACCATGGCAGCCTGGCCAGCCGGCAGATGATCCAGCAGTATGAAATGTACTGTAAAGAC TCACGGGGTCGCCTCATCCCTGGTGCATATAAGTTTGATGCCTTGATCACCACTTTTGAGATGATTTTGT CAGACTGTCCTGAGCTTCGTGAAATTGAATGGCGTTGTGTTATCATTGATGAAGCCCATAGGCTGAAGAA CCGTAATTGCAAGCTACTTGATAGTCTCAAGCACATGGACCTGGAGCATAAAGTGTTACTCACAGGGACA CCGTTACAAAATACCGTAGAGGAACTGTTCAGTCTACTGCATTTCTTGGAACCTTCTCAGTTCCCCTCAG AATCAGAATTCCTTAAGGATTTTGGGGATCTCAAGACCGAGGAGCAGGTTCAAAAGCTTCAGGCCATTCT AAAGCCAATGATGCTGAGGAGACTCAAAGAGGATGTTGAAAAAAATTTGGCTCCCAAACAGGAAACTATT ATTGAAGTAGAGCTAACCAACATCCAGAAGAAATACTACAGAGCTATTTTAGAGAAGAATTTCTCCTTCC TTTCCAAAGGGGCAGGTCATACCAATATGCCTAATCTACTGAACACAATGATGGAGCTACGAAAGTGCTG CAATCACCCGTACCTCATCAATGGTGCAGAAGAGAAAATCCTGATGGAATTTCGGGAAGCTTGCCATATT ATACCTCAAGATTTCCACCTGCAGGCTATGGTTCGGTCAGCTGGCAAGTTAGTTCTCATTGACAAGTTAC TTCCAAAGCTTAAAGCTGGTGGCCATAAAGTTCTGATCTTCTCCCAGATGGTACGCTGTCTAGACATTCT GGAGGATTATCTGATCCAGAGGAGGTACTTATATGAACGTATTGATGGGCGAGTTAGAGGCAACCTTCGA CAAGCTGCTATCGACCGCTTTAGCAAGCCTGACTCAGACCGCTTTGTCTTCTTGCTATGTACCCGTGCTG GTGGACTTGGTATTAATCTTACAGCTGCTGATACCTGTATTATCTTTGATTCAGACTGGAATCCACAGAA TGACCTGCAGGCCCAAGCACGTTGTCATCGAATTGGACAGAGCAAAGCTGTGAAGGTGTACCGACTCATC ACTCGTAATTCCTATGAGAGAGAGATGTTTGATAAAGCTAGCCTCAAGTTGGGATTGGATAAAGCTGTGC TTCAGTCCATGAGTGGTCGGGATGGCAACATTACTGGAATTCAACAGTTCTCCAAGAAAGAGATTGAAGA TCTCTTAAGGAAAGGTGCCTATGCGGCCATAATGGAGGAAGACGATGAGGGTTCTAAGTTTTGTGAGGAG GACATAGATCAGATCTTACTGAGACGGACCACAACCATCACTATTGAGTCTGAAGGGAAAGGTTCTACTT TTGCCAAGGCAAGCTTTGTTGCTTCTGAAAATAGGACAGATATTTCCCTGGATGATCCAAACTTTTGGCA AAAATGGGCCAAAAAGGCAGACCTAGACATGGATCTGCTGAATAGTAAGAATAACTTAGTGATCGACACA CCTCGAGTTCGGAAACAGACTCGACACTTCAGCACTTTGAAAGATGATGACCTGGTTGAGTTTTCTGATT TGGAAAGTGAAGATGATGAACGGCCACGTTCCCGAAGACATGACCGGCATCACACCTATGGGCGCACTGA CTGCTTCCGGGTAGAAAAGCATCTCCTGGTATATGGTTGGGGACGTTGGAGAGATATTTTGTCTCACGGA CGATTTAAGCGGCGCATGACTGAAAGAGATGTTGAGACAATATGCCGTGCCATCCTCGTGTACTGTCTTC TACACTACCGTGGGGATGAAAACATCAAAAGCTTCATTTGGGACTTGATTAGCCCTGCTGAAAATGGCAA GACAAAAGAATTGCAGAATCATTCAGGTCTGTCTATCCCTGTGCCCCGTGGGCGTAAGGGGAAAAAAGTC AAGTCACAAAGTACTTTTGACATTCATAAGGCAGACTGGATCCGGAAATACAACCCTGATACTCTGTTCC AAGATGAGAGTTATAAGAAGCATTTGAAACATCAGTGTAACAAGGTACTGTTGAGAGTCCGGATGCTCTA CTACCTGAGACAGGAGGTTATTGGAGACCAGGCAGAGAAGGTGTTAGGAGGTGCCATAGCCAGTGAGATT GACATATGGTTCCCAGTGGTGGATCAGCTGGAGGTTCCTACAACTTGGTGGGATAGTGAGGCTGACAAAT CCCTGCTCATTGGCGTTTTTAAGCATGGCTATGAGAAATACAATACCATGAGGGCAGACCCTGCCTTGTG CTTCCTAGAAAAGGCTGGCAGGCCAGATGACAAAGCCATCGCAGCAGAACATAGAGTGTTGGATAATTTC TCCGACCTGGTAGAAGGGATTGACTTTGATAAGGATTGTGAAGATCCTGAATATAAACCCCTCCAGGGTC CTCCAAAGGACCCAGATGATGAGGGTGATCCCTTGATGATGATGGATGAGGAGATCTCAGTCATCGACGG AGAGGAAGCCCAGGTAACTCAACAGCCAGGGCATTTATTCTGGCCTCCAGGCTCCGCCCTCACAGCTAGG CTTCGCCGCCTAGTAACAGCCTATCAACGCAGCTACAAGAGAGAACAAATGAAGATGGAGGCTGCGGAAC GTGGGGACCGGAGACGCCGACGATGTGAAGCAGCCTTTAAGCTCAAAGAAATCGCAAGGCGGGAAAAACA ACAACGGTGGACAAGGCGTGAGCAAACTGATTTCTACCGAGTAGTATCTACCTTTGGTGTGGAGTATGAC CCTGACAACATGCAGTTTCACTGGGATCGCTTCCGCACTTTTGCTCGCCTGGACAAAAAAACAGATGAAA GCCTTACCAAGTACTTCCATGGTTTTGTGGCCATGTGTCGACAAGTGTGCCGCCTTCCCCCAGCTGCTGG TGATGAACCTCCAGACCCTAATCTGTTCATTGAGCCCATCACTGAAGAGAGAGCCTCGAGGACTCTCTAC CGAATTGAGTTGCTTCGACGCTTACGGGAACAAGTTTTATGCCACCCCCTTTTAGAAGATCGGCTGGCAT TGTGTCAGCCTCCAGGTCTTGAATTGCCCAAATGGTGGGAACCTGTCCGTCATGATGGGGAGCTCCTACG AGGAGCAGCCCGCCATGGGGTGAGCCAAACAGACTGCAACATCATGCAGGACCCAGACTTCTCTTTTCTG GCTGCCCGTATGAATTATATGCAGAACCATCAGGCGGGAGCATCAGCTGCATCCCTGTCGCGATGCTCCA CTCCACTGCTGCACCAGCAGTGTACCTCACGTACTGCTTCACCATCACCCCTGCGCCCAGATGCTCCTGT TGAAAAGTCACCTGAGGAAAGTACTGTTCAGGTCCCCAATCTGGAGAGTCTGACTTTAAAGTTAGAGGAT GAGGTTGTGGCTAGGAGCCGACTCACCTCACAAGACTATGAAGTACGCGTAGGCTCTTCAGATACAGCTC CTCTGTCCCGGAGTGTCCCACCAGTGAAACTGGAGGACGAGGATGATTCAGACTCTGAGCTGGACTTGAG CAAGTTGTCACCATCCTCCTCCTCTTCTTCATCCTCGTCCAGCTCCAGCTCCAGCACTGACGAGAGTGAA GACGAGAAGGAAGAGAAGTTAACTGCTGACCGGTCCCGCCCAAAGCTCTATGATGAAGAGAGCCTTCTGT CCCTTACTATGTCCCAAGATGGATTCCCAAATGAAGATGGAGAACAAATGACCCCTGAGTTGCTGCTACT GCAGGAAAGACAAAGAGCCTCTGAATGGCCTAAGGATCGTGTTCTGATAAACCGCATTGACCTCGTCTGC CAGGCTGTACTCTCAGGGAAATGGCCTTCTAACCGCCGGAGCCAGGAAGTGACAGCAGGAGGAATTTTGG GGCCAGGCAACCATTTATTAGACAGTCCCTCTTTGACCCCAGGAGAAGATGGGGACTCTCCAGTCCCCAC GCCACGAAGTGGCAGTGCAGCTTCCATGGCGGAGGAAGAGGCATCTGCAGTCACCACAGCGGCGGCCCAG TTTACGAAACTTCGGCGAGGCATGGATGAGAAAGAGTTTACAGTTCAGATCAAAGATGAAGAAGGATTGA AGTTAACATTCCAGAAGCATAGATTGATGGCTAATGGTGTAATGGGAGATGGACATCCACTGTTTCATAA GAAGAAGGGGAACAGGAAAAAGCTAGTAGAGCTGGAGGTGGAGTGCATGGAAGAGCCTAATCACCTTGAT CTGGACCTGGAGACCCGGATCCCTGTCATCAATAAGGTGGATGGTACTTTGCTGGTGGGTGATGAGGCCC CTCGCCGGGCGGAGCTGGAGATGTGGTTACAGGGTCATCCAGAGTTTGCTGTTGATCCCCGATTTCTAGC GTATATGGAGGAACGTAGAAAACAGAAGTGGCAGAGATGTAAAAAAAATAATAAGGCAGAATTGAACTGT TTGGGAATGGAACCAGTACAGCCAGCGAACTCTAGAAATGGGAAAAAGGGTCACTATGCTGAAACTGCGT TCAACCGGGTTTTGCCAGGGCCTGTTGCACCAGAGAACAGCAAGAAACGGGTCCGTAGGACAAGACCAGA CCTTTCTAAGATGATGGCCCTGATGCAGGGTGGAAGCACTGGGTCCCTGTCTCTGCATAACACCTTTCAA CACAGCAGTAGTAACCTACAGTCTGTGTCGTCTCTGGGTCACAGCAGTACCACTTCTGCATCTCTGCCTT TTATGCCGTTTGTGATGGGTGCAGCAGCACCCCCTCATGTAGACTCCAGCACCATGCTTCATCATCACCA CCACCATCCCCACCCCCACCATCACCACCATCACCATCCAGGCTTGAGAACCACTGGCTACCCTTCTTCA CCAGCTACTACCACCTCTGGTACTGCCTTGCGGTTACCAACACTGCAACCTGAGGACGATGATGAAGAGG AGGATGAAGAAGATGATGATTTATCTCAGGGCTATGACAGCTCAGAACGGGACTTCTCACTCATTGATGA CCCTATGATGCCAGCCAACTCAGACTCCAGTGAAGATGCTGACGACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_201637
Insert Size	7749 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_201637.2, NP_963999.2
RefSeq Size	8190 bp
RefSeq ORF	7749 bp
Locus ID	67772
UniProt ID	Q09XV5
Gene Summary	This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which is common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. In mammals, this gene has been shown to function in several processes including transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Knockout of this gene causes early embryonic lethality due to widespread apoptosis. Heterozygous loss of function mutations result in autism spectrum disorder-like behaviors that include increased anxiety, repetitive behavior, and altered social behavior. [provided by RefSeq, Dec 2016]

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