Muc5ac (NM_010844) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210005L13Rik; MGM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225400 representing NM_010844 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTCGGCCGGAGAAAGTTGGTCCCATTCTGGGTCCTAGCCCTGGCCCTGGCCTGCAGCCAATGCA CAGGCCAGGCTCAACAGGACTCTCTGAAATCGTACCATGAACACCGCTCTGATGTTCCTCACCCTCAAGG GCATGTTGGTACCCCACTCAATAGGGTGACCATCATCCCACCTCTGAAGACCATCCCTGTGGTACGAGCC TTCAACCCAGGACATACCAGGCGGGTGTGCAGCACATGGGGCAACTTCCACTACAAGACCTTTGATGGAC AGGTCTTCTACTTCCCTGGTCTCTGCAACTATGTGTTCTCTGCACACTGTGGGGATGCCTATGAGGACTT CAATATCCAGCTACGCCGTGTCCAGGAGTCTAATACCACCACTCTGAGTAGGGTCACCATGAAGCTTGAT GGCCTAGTTGTTGAGCTGACCAAGAGCTCCGTCTTAGTCAATAACCACCCGGTCCAGCTGCCCTTTAGCC AGTCTGGGGTCCTCATTGAGCTCAGCAATGGCTACCTGAAGGTGGTGGCTAGGCTGGGGCTGCTCTTCGT GTGGAACGAGGATGACAGTCTTCTGCTGGAGTTGGACACCAAATATACCAACAAGACTTGTGGTCTCTGT GGAGACTTCAACGGCAGTCCAAAATCCAACGAGTTCCTCTCCAACAATGTTAGGCTGACACCCCTTGAGT TTGGGAACCTTCAGAAGATGGACGGCCCCACAGAGCAGTGCCAAGACCCCCTCCCTGTGCCCCAGAAGAA CTGCTCCGCCAGATCTGGTATCTGTGAAATGATCCTAAAAGGTGAGCTGTTCTCAGGCTGTGCGGCCCTG GTGGACATCAGCAGCTACGTGGAGGCTTGCCGGCAGGACGTCTGTCTCTGTGAGAGCTTGGACCCGTCTG ACTGCATCTGCCATACCCTCGCTGAGTACTCCCGGCAGTGTGCACATGCTGGAGGGCAGCCCCAGGACTG GCGGGGCCCCAACCTCTGCTCCCAGACATGTCCCCTCAACATGCAGCACCAGGAATGTGGCTCACCCTGT GTGGACACCTGCTCCAACCCCCAGCATTCCCAGGTCTGTGAGGACCACTGTATTGCTGGCTGCTTCTGTC CTGAGGGTATGGTGCTTGATGACATTAATCAGATGGGCTGTGTTCCTGTGTCCCAGTGTGCCTGCCTGTA CAATGGGACACTTTATGCACCGGGTACCAATTACTCTACTGACTGCACCAACACATGTTCTGGAGGGCAG TGGAGCTGCCAGGACATCCCCTGCGCTGGCACCTGCTCAGTAATGGGAGGCTCCCACATGTCCACATTTG ATGGAAGGCAGTACACAGTACATGGGGACTGCACCTATGTGCTGAGCAAGCCTTGTGACAGTAATGCCTT CACTGTGCTGGTTGAGCTGCGAAAGTGTGGACTGACGGAAAGTGAGACTTGTCTGAAGACTGTGACATTG AACCTGGGTGGGGGGCAGACGGAGATCATGGTGAAAGCTACTGGAGAGGTCTTTGTGAACCAGATCTACA CCCAGTTGCCAGTGTCTACAGCCAATGCTACCTTCTTCCGGCCTTCAACCTTCTTCATCGTTGGTGAGAC CAACCTGGGTTTGCAGCTTGAGATCCAGCTGAGTCCAATTATGCAAACATCTGTGCGTCTGAAACCTGGG CTCAGGGGGCTAACCTGTGGGCTCTGTGGTAACTTCAACAGTATGCAGGCTGATGACTTCCAGACTATCA GTGGGGTTGTGGAGGGCACAGCAGCTGCTTTCTTCAACACCTTCAAGACTCAGGCGGCTTGTCCCAATGT CAAGAATATCTTTCAGGACCCCTGCTCGCTCAGCGTGGAGAATGAAAAGTATGCTCAGCACTGGTGTTCT CTGCTGACTAACGCCAGTGGTCCATTTTCCCAGTGCCACGCCACCGTGAACCCCAGCACCTTCTTCTCGA ACTGCATGTATGACACGTGCAACTGTGAGAAGAGCGAGGACTGCATGTGTGCAGCCCTGTCCTCCTATGT GCGTGCTTGTGCTGCCAAAGGCGTGCTGCTCAGTGACTGGAGGGATGGCATCTGCACAAAGCCTACAATT ACCTGTCCCAAGTCAATGACCTATCAATACCATATCAGCACCTGCCAGCCCACCTGCCGTGCTCTGAATG AGAAAGATGTCACCTGCCATGTCAGCTTCATCCCTGTAGATGGCTGCACCTGCCCCAAAGGCACCTTCTT AGATGATTTGGGCAAATGTGTACAGGCTACCAGCTGTCCTTGCTACTACAAGGGATCCACAGTTCCCAAT GGCGAGTCTGTGCAGGACAGTGGGGCCATTTGCACCTGCACCCAAGGAGCACTAACTTGTATTGGAGGTC CTGCCCCGACTCCAGTGTGTGATGCACCCATGATCTATTTTGACTGTCACAATGCCACACCTGGTGACAC TGGAGCTGGATGTCAGAAGAGCTGTCACACCCTAGACATGACCTGTTATAGCTCCGAGTGCGTGCCTGGC TGTGTGTGCCCCAATGGGCTGGTGGCAGATGGAAATGGAGGCTGTGTTGTTACTGAGGACTGTCCTTGTG TGCACAATGAGGCCACCTATAGGCCTGGGGAGACCATCCAAGTGGGATGCAACAACTGCACCTGTGAGAA CAGGATGTGGCAATGCACAGACAAGCCTTGCCTGGCCACCTGTGCTGTGTATGGAGATGGCCACTACATC ACTTTTGATGGGCAGCGCTACAGTTTCAATGGGGACTGCGAGTACACACTGCTACAGGACAACTGTGGTG GGAATGGCAGCTCCCAGGATGCCTTTCGTGTTATCACTGAGAACATCCCCTGTGGTACTACAGGAACCAC CTGCTCCAAGAGCATCAAGATCTTCCTGGGGAACTATGAGCTGAAGTTGAGTGACAGCAAGATGGAGGTG GTCCAGAAGGATGTGGGGCAGGAGCCCCCTTACTTTGTCCACCAGATGGGCAACTACCTGGTGGTGGAAA CTGACATTGGCCTGGTGCTTTTGTGGGACAAGAAGACTAGCATCTTTCTCAGACTCAGCCCTGAGTTCAA GGGCAGGGTCTGTGGCCTGTGTGGGAACTTTGATGACAATGCCATCAATGACTTCACCACACGCAGCCAG TCCGTGGTCAGTGACATGTTGGAGTTTGGAAATAGCTGGAAGTTGTCTCCATCCTGCCCGGATGTCCTGG TGCCCAAGGACCCCTGCACTGCCAACCCTTACCGCAAGTCCTGGGCCCAAAAGCAATGCAGCATCATCAA CAGCGAAACTTTCTCCGCCTGCCATGCTCATGTGGAGCCGGCCAAGTACTATGAAGCTTGTGTGAATGAC GCCTGTGCCTGTGACTCAGGGGGTGACTGCGAGTGTTTCTGCACCACTGTGGCCGCCTATGCCCAGGCCT GCCATGAAGTGGGAGTGTGTGTGTCCTGGAGGACACCAGACATCTGCCCACTGTTCTGTGACTACTACAA CCCAGAGGGTCAGTGTGAGTGGCACTACCAGCCGTGTGGGGCCCCCTGCATGCGTACCTGCCAGAACCCT ACTGGACAGTGCCTACAAGATCTCCGTGGTCTGGAAGGATGCTATCCCAAGTGCCCACCAACAGCCCCCA TCTTTGATGAGGGCACAATGCAGTGTGTATCCAACTGTACAGTCACCTTCCCCTGCCGCGTCAATGGAAA GTTGTACCGGCCAGGTGCATCAGTACCTTCAGACAAAAACTGCGATTCCTGCATCTGCACGGAGAGTGGT GTGCGCTGTACCCACAATGCTGGTGCCTGTGTCTGTACCTACAACGGGCAACAGTTCCATCCTGGGGAGA TCATCTACCACACAACAGATGGCATAGGAGGCTGCATCTCTGCACACTGCAGGGCCAATGGTACCATCGA GAGGAGCGTTGACACCTGCAACTCCACCACCCCTACACCCCCCACCACTTTCTCCTTCTCCACACCGCCC GTCATGACCTCAATGCAACCCTCCAGCACACATTCCAGCCCTACCCCGAGTGTAGGGTCCTCAGGGGCCT CAAGCAAGGCTGCATCGACAACCAGCAGCATATTGTCTGTGAAGAGCCCTGTTACAGCTCCTATGACCAT GTCTACTTCAGCCTCCGCCGTAACCACATCAGGTTGCCGGGAGGAGTGCCTCTGGTCTCCCTGGATGGAT GTTAGCCGTCCTGGACGTGGCATTGACAGTGGTGACTTTGACACTCTGGAGAACCTCCGTGCCCATGGCT ACCCAATCTGCCAAGTGCCAAAAGCAGTAGAGTGCCGTGCTGAGGCTAGCCCCGGGGTGCCTCTTCCCGA GCTGCAGCAGCACCTGGAGTGTAGCACAACAGTGGGGCTGATCTGTTACAACAGTGATCAGCTGTCAGGG CTCTGTGACAACTACCAGATCAAAGTCCAGTGCTGTACCCCCGTCAGCTGTCCAACCTCCCAGACGACCC ATGTGATATCATCCTCCAGGACGACTAATTTGGATAACACAACCTCCTCAGTCCCTGTTACCTCAACTGA GCACCCATACAGCAGTACAGTTACCTCAGGTTCTTCTACCCACACCCCAGGTCTGTCTCCCTCTTCCTCA GTGCCTTCTTCACCAACTCCAGCTTCATCTACTCCTGCCCCAGTCAGTTCTACCACTGTTAAAACAACTC TGCCCATCACTAGCCCAACACCAGAGCCAACTCCAGCCATATCGTCTGTGTCCATCTCAACCTCAGGGTC CACCATGCCTTCCTCTGAAACAACTCATGAGTGCAAACAGGAGCTTTGCAATTGGACCAATTGGCTAGAT GGCAGTTACCCTGGGTCTGGCAGAAACAGTGGAGATTTTGACACCTTTGTGAACCTGAGATCCAAAGGAT ACAAGTTCTGTGAGAAGCCACGAAATGTTGAGTGCAGGGCTCAGTTCTTTCCCAACACACCACTGGAGGA GCTGGGGCAGAATGTGACCTGCAGCCGAGAGGAGGGTTTGATCTGTTTGAACAAGAACCAGCTGCCACCC ATGTGCTACAACTATGAGATCCGGATTGAGTGCTGCACAGTGGTAAACAACTGTTCCACAGCTTCAGTTA CCACACATCCCACCTCACATGGAGTCAGCACGAAAACAGAGACCAACTGGACCACCCATGTGTATTCCTC TCCCACAAAAGACACCAGTAGTCACTCAGCAACCATAGACACAAAGACCTGGACCTCAGGTATTTCACAC ACAACCACTCAACCAGTGACCACCCACTGCCAGCTACAGTGCAACTGGACCAAGTGGTTTGACACTGACT TCCCAGTGCCCGGGCCACATGGAGGGGACCTGGAAACCTATAGCAACATTGAGAGGAGCGGAGAGAGACT CTGTCACCGAGAGGAGATCACACAGTTGCAATGCAGGGCTAAGAACTACCCTGAGAGAGAGATGGAGGAT CTGGGTCAGGTGGTGAAGTGTGACCCCAGTGTGGGCCTGGTGTGCAACAACAGGGACCAGGGGGGCGACT CTGGGATGTGCCTCAACTATGAGGTGCGACTGCTGTGCTGTCACATTCCTGAAGACTGCCCCAGGACTGA TCAGACTTCTCCTGTGACATTATCCCATAAGCCCTCATCTGCAGTTGTGTCACCATCATCTGTGTCCCCG TCCTTGTCGACCAGCCATAGGGTTCACTCTACCACTCCCTGCTTCTGCAGCGTGTCAGGCCAGCTATACC CCTTGGGATCCATCATCTACAACCAGACAGACCTTGATGGCCACTGTTACTATGCGATGTGTAGCCAGGA TTGTCAGGTGGTCAAGAGGGTTAGTCAAGACTGCCCCTCCACCATGCCACCCCCTGCGACAACTCTATCT ACATCTACCACGCCACCTGTCACTGGGCGGGATCGGTGCAATGTGTTTCCTCCAAGACTGAGAGGAGAGA CCTGGCCAATGCCGAATTGTTCCCAAGCCACCTGTGAGGGCAACAATGTCATCTCCTTGAGCCCACGCCA GTGCCCAGAGCTGAATGAACCATCTTGTGCCAATGGTTACCCACCTCTGAAGGTGGATGACCAGGATGGT TGCTGCCAGCACTACCAGTGCCAATGTGTTTGCAGCGGGTGGGGTGATCCCCACTACATCACATTTGATG GCACCTACTACACTTTCCTGGATAACTGCACATACGTGCTGGTGCAGCAGATCGTGCCCGTGTTTGGATA CTTCCGTGTGCTCATTGACAACTACTACTGTGACGTGGGAGACAGCGTCTCCTGCCCACAGTCCATCATT GTGGAGTACCACCAGGACCGTGTGGTGCTGACCCGCAGGCCAGTTAGCGGGGTCATGACCAACCAGATCA TCTTCAACAACAAAGTAGTCAGTCCTGGCTTCCAGCAGAATGGCATTGTCACCTCCCGTGTGGGTATCAA GATGTATGTTACCATCCAAGAGATTGGTGTCCGGGTCATGTTTTCAGGTCTCATCTTCTCCGTCGAGGTG CCTTTCAACTTGTTTGCCAACAACACAGAGGGCCAGTGCGGCACTTGCACCAATGACAAAAAGGATGAGT GCCGCCTGCCTGGGGGTTCCATAGCCTCCTCTTGTTCTGAGATGTCCCTCCACTGGAAGGTGCCCAACCA GCCTTCCTGCCAAGGGCCTCCACCAACTCCAACTTCAGTGGTACCCAGGCCTTCACCTACTCCGTGCCCA CCATCGCCACTCTGTGAGCTCATCCTAAGCAATACCTTCAAGCTCTGCCATGACGTCATCCCCCCGCTGC AGTTCTATCAAGGCTGCTTATTTGACTACTGCCACATGCTGGACCTGGAGGTTGTATGCTCAGGCCTAGA GCTCTATGCATCACTCTGTGCAGCCCAAGGCGTGTGCATTCCCTGGAGAAGCCAAACCAACAACACCTGC TCATTCACGTGCCCTGATAACCAAGTATACCAGCCTTGTGGCCCATCCAACCCTCACTACTGCTACAGGG ATGATAGCATCAGCCCAAGCCTGACCCTTCAAGAGGCTGGTCCCAAGACAGAGGGCTGCTTCTGTCCAGA CAGCACGACACTTTTCAGTACCAATGACTCAATCTGCGTGCCTTCCTGCCAATGGTGTCTGGGGCCTCGT GGGGAGCCTGTGGAGCCGGGTCACACCATTAGCATCGACTGCCAGGACTGCATCTGTAAGGAAGCCACGC TAACCTGCCAAAAGAAGGCCTGTCCTCAGCCCACCTGTCCAGAGCCTGGCTTTGTGCCAGTGCCTGTAGC CCTGGAGGCTGGCCAATGTTGTCCCCAGTTCAGCTGTGCCTGCAACTCCAGCCACTGCCCTCCGCCTCTG CACTGCCCGAAGAATTCTAGCCTGATAGTCACATACGAGGAGGGAGCTTGCTGCCCGACCCAGAACTGCA GCAGTCAGAAGGGCTGTGAAGTCAACGGGACTCTCTACCAGCCTGGTGACGTGGTCTCCTCCAGCCTATG TGAGAGATGCCTGTGTGAGGTTTCCAGCAATCCCCTTTCCGATGTCTTTATGGTCAGCTGTGAGACTGAG CTCTGTAACACCCAGTGTCCTAAGGGCTCTGAGTACCAGGCCATGCCAGGGCAGTGCTGTGGCAAGTGTA TACCGAAGACCTGCCCTTTTAAAAACAACAGTGGTTCTACCTACTTCTACCAGCCTGGTGAGTTATGGGC AGAACCTGGGAACCCGTGTGTGACTCATAAGTGTGAGAAATTCCAAGATGTACTCATGGTCGTGACAATG AAGACAGAGTGTCCCAAGATCAACTGTCCGCAGGGCCAGGCTCAGCTGAGAGAAGACGGATGCTGCTATG ACTGTCCCTTACCTAACCAGCAGAAATGTACGGTGCACCAAAGACAGCAGATCATCCGTCAGCAAAACTG CAGCTCTGAGGGCCCAGTGAGCATCTCCTACTGCCAAGGCAACTGCGGGGACAGCATCTCCATGTACTCC CTGGAGGCCAACAAGGTAGAGCACACATGTGAGTGTTGCCAGGAGCTGCAAACCTCTCAGAGGAATGTGA CTCTGCGCTGCGATGATGGCTCCAGTCAGACCTTCAGCTACACCCAGGTTGAGAAGTGTGGCTGCCTGGG CCAGCAGTGCCATGCTTTAGGGGACACAAGCCATGCAGAGTCCTCAGAACAAGAGTTCAAGTCCAAGGAA AGTGAGGAACATGGCCAGCAGTTGGCATTCAGGGTCAGCGAGGACATGCTTGGGCCTTTCCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010844
Insert Size	8256 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010844.1, NP_034974.1
RefSeq Size	8505 bp
RefSeq ORF	8256 bp
Locus ID	17833

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