Ubr5 (NM_001112721) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Edd; Edd1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225409 representing NM_001112721 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCCATCCATTTCGTGGTCCACCCGCTGCCGGGCACCGAGGACCAGCTCAATGACAGGTTACGGG AAGTTTCAGAGAAGCTGAACAAGTACAATTTGAACAGCCACCCACCTCTGAATGTACTGGAGCAGGCTAC TATTAAACAGTGTGTGGTGGGACCCAATCACGCCGCGTTTCTTCTTGAGGATGGCAGAATTTGTAGGATT GGTTTTTCAGTACAACCAGACAGACTGGAATTGGGTAAACCTGATAATAATGATGGGTCAAAACTGAACA GCAGCTCGGGGACAGGGAGGACCTCTAGGCCAGGAAGGACAAGTGACTCCCCGTGGTTTCTCTCAGGTTC TGAGACTCTGGGCAGGTTGGCAGGCAACACTCTGGGAAGCCGCTGGAGTTCTGGCGTGGGGGGAAGTGGA GGAGGCTCCTCTGGTCGGTCGTCTGCTGGAGCTCGTGATTCCCGGCGACAGACTCGAGTCATTCGGACTG GCCGGGATCGGGGGTCTGGACTCCTGGGCAGTCAGCCCCAGCCAGTTATTCCAGCATCTGTCATTCCTGA GGAGCTGATATCACAGGCACAAGTTGTTCTACAAGGCAAATCGAGAAGTGTCATTATTCGAGAACTTCAG AGAACAAATCTTGATGTCAACCTTGCTGTAAATAACTTACTGAGCCGGGATGATGAGGATGGAGATGACG GTGATGACACAGCCAGTGAATCTTACCTGCCGGGAGAAGATCTTATGTCTCTTCTTGACGCTGACATTCA CTCTGCCCACCCAAGTGTCATTATTGATGCTGATGCCATGTTTTCTGAGGACATTAGCTATTTTGGTTAT CCTTCTTTTCGTCGTTCATCACTCTCTAGGCTAGGCTCATCACGAGAGAGAGACTCAGAGCTTCTGCGGG AACGGGAGTCTGTTCTACGTTTACGGGAGCGGCGGTGGCTTGATGGAGCCTCATTTGATAATGAAAGGGG CTCTACCAGCAAAGAGGGAGAGTCAAACCCGGATAAGAAGAACACGCCTGTTCAGAGTCCAGTGTCTCTG GGAGAAGACTTACAGTGGTGGCCTGATAAGGATGGAACAAAATTTACCTGCATTGGGGCTCTGTATTCTG AGCTTCTTGCTGTCAGCAGCAAAGGAGAACTTTATCAATGGAAATGGAGTGAATCTGAGCCTTACAGAAA TGCCCAGAATCCTTCATTGCATCATCCACGAGCAACATTTTTGGGGTTGACCAATGAAAAGATAGTCCTC TTGTCTGCAAATAGCATAAGAGCAACTGTAGCTACTGAAAATAACAAGGTTGCTACATGGGTGGATGAAA CATTAAGTTCTGTGGCTTCTAAGTTAGAACACACAGCACAGACTTACTCTGAACTTCAAGGAGAACGGAT AGTTTCTTTACATTGCTGTGCCCTTTATACCTGTGCCCAGCTGGAGAATAATTTGTATTGGTGGGGTGTT GTTCCTTTCAGTCAAAGGAAGAAAATGTTAGAGAAAGCTAGAGCAAAAAATAAAAAGCCCAAATCCAGTG CTGGTATTTCTTCCATGCCAAACATCACTGTTGGAACTCAGGTGTGCTTGAGAAATAACCCTCTCTACCA TGCTGGAGCGGTTGCCTTTTCAATTAGTGCTGGCATTCCTAAAGTTGGTGTCTTGATGGAGTCAGTATGG AATATGAATGATAGCTGTAGATTTCAACTTCGGTCTCCAGAGAGCCTGAAGAGCATGGAAAAAGCCAGCA AAACTCTTGAGACAAAGCCCGAGAGTAAGCAGGAACCAGTAAAAACAGAAATGGGCCCTCCACCATCACC AGCATCCACCTGCAGCGACGCGTCCTCAATTGCCAGCAGTGCATCCATGCCATACAAACGACGTCGGTCA ACTCCTGCCCCGAGAGAAGAGGAGAAGGTGAACGAGGAGCAGTGGCCCCTTCGGGAAGTTGTTTTTGTTG AAGATGTTAAGAACGTTCCTGTTGGCAAGGTGCTGAAGGTAGATGGTGCCTATGTCGCTGTAAAGTTTCC AGGAACATCTACTAACACCACCTGTCAGAACAGCTCTGGTCCAGACGCCGACCCTTCCTCGCTCCTGCAG GACTGCAGGCTGCTTCGAATTGATGAGCTGCAGGTTGTCAAAACGGGCGGCACCCCAAAAGTTCCCGACT GTTTTCAGAGGACTCCCAAAAAGCTTTGTATACCCGAGAAGACTGAAATACTAGCAGTAAATGTGGATTC CAAAGGTGTCCATGCTGTTCTGAAGACTGGAAGCTGGGTTCGGTACTGTGTCTTTGATCTTGCCACAGGG AAGGCTGAACAAGAGAATAATTTCCCAACAAGCAGTGTTGCTTTTCTTGGTCAGGATGAGAGGAGTGTCG CTATCTTCACTGCTGGGCAGGAATCTCCTATTGTTCTTCGAGATGGAAATGGTACTATCTACCCAATGGC CAAGGATTGCATGGGAGGAATACGGGATCCTGACTGGCTTGATCTCCCACCAATCAGTAGTCTTGGGATG GGTGTACATTCTTTAATAAATCTTCCTGCCAACTCAACAATCAAAAAGAAAGCAGCTATTATCATCATGG CTGTAGAGAAGCAAACCTTAATGCAGCACATTCTCAGATGTGACTACGAGGCCTGCCGACAGTATCTCGT GAATCTGGAGCAAGCCGTGGTTTTAGAGCAGAACCTGCAGATGTTACAGACGTTCATCAGCCACAGATGT GATGGGAACCGGAACATTTTACATGCTTGTGTATCAGTTTGCTTTCCAACCAGCAATAAGGAAACTAAAG AAGAAGAGGAAGCTGAGCGCTCAGAAAGAAATACATTTGCTGAAAGGCTTTCTGCTGTGGAGGCCATTGC AAATGCAATATCAGTTGTTTCAAGTAACGGCCCAGGTAATCGGGCTGGATCATCAAATAGTCGAAGTTTG AGATTACGAGAAATGATGAGACGTTCATTGAGAGCAGCTGGTTTGGGTAGACATGAGGCTGGAGCTTCAT CCAGTGACCACCAGGATCCAGTTTCACCCCCCATAGCTCCCCCTAGCTGGGTTCCTGACCCTCCTTCAAT GGATCCTGATGGTGATATTGATTTTATCCTGGCCCCCGCTGTGGGATCTCTTACCACAGCAGCGACTGGC AGTGGTCAAGGACCCAGCACCTCCACCATTCCAGGTCCTTCCACAGAGCCATCTGTAGTAGAATCCAAGG ATCGAAAGGCAAACGCACATTTCATATTAAAATTACTATGCGACAGTGCGGTTCTGCAGCCTTATCTACG AGAGCTTCTTTCTGCCAAGGATGCAAGAGGAATGACCCCGTTTATGTCAGCTGTAAGTGGCCGGGCTTAT CCTGCTGCAATCACCATCCTAGAAACAGCCCAGAAGATTGCCAAAGCTGAAGTATCTGCAAGTGAAAAGG AGGAAGATGTGTTCATGGGAATGGTTTGCCCGTCAGGTACCAACCCTGACGACTCCCCTTTGTATGTCTT ATGTTGCAACGACACCTGCAGCTTTACCTGGACTGGAGCAGAGCACATTAACCAGGACATTTTTGAATGT CGGACTTGTGGTTTACTGGAGTCGCTTTGTTGTTGTACAGAATGTGCAAGGGTTTGTCATAAAGGGCACG ATTGCAAACTCAAGAGGACTTCACCAACAGCTTATTGTGATTGTTGGGAGAAATGTAAATGTAAAACATT GATTGCTGGACAGAAGTCTGCTCGTCTTGATCTACTTTATCGCCTGCTTACTGCTACAAATCTGGTCACC CTACCAAACAGTAGGGGAGAGCACCTTTTGCTGTTCTTAGTACAGACCGTGGCCAGGCAGACAGTGGAGC ACTGTCAGTATAGGCCTCCACGAATCAGGGAGGATCGCAACAGGAAAACAGCCAGTCCTGAAGATTCAGA TATGCCTGACCATGACTTAGAGCCCCCAAGGTTTGCCCAGCTTGCCCTAGAGCGCGTCCTTCAGGACTGG AATGCTTTGAGATCTATGATTATGTTTGGGTCACAAGAGAACAAAGATCCTCTGAGTGCCAGCAGCAGGA TAGGCCATCTTTTGCCAGAAGAGCAGGTCTACCTCAACCAGCAGAGCGGCACGATTCGGCTCGACTGTTT CACTCATTGCCTTATTGTCAAGTGTACAGCAGATATTTTGCTTTTAGATACTCTGCTAGGCACCTTAGTA AAAGAACTCCAAAACAAGTACACACCCGGACGCCGGGAAGAAGCAATTGCCGTGACAATGAGGTTTCTAC GATCTGTGGCAAGAGTGTTTGTCATTCTTAGTGTGGAAATGGCTTCGTCCAAAAAGAAAAACAACTTTAT TCCACAGCCAATTGGAAAATGCAAACGTGTGTTTCAAGCACTGCTTCCTTATGCTGTAGAAGAATTGTGT AATGTGGCAGAGTCTCTGATTGTTCCTGTCAGAATGGGGATTGCCCGGCCGACAGCACCGTTTACTCTGG CCAGTACTAGCATAGATGCCATGCAGGGCAGTGAAGAATTATTTTCAGTAGAACCATTACCACCACGGCC GTCATCAGATCAGGCAAGCAGCTCCAGTCAGTCTCAGTCATCTTACATCATTCGGAACCCACAGCAGAGG CGCATCAGCCAGTCACAGCCCGTCAGAGGCCGAGACGAGGAGCAGGATGATATTGTGTCAGCAGACGTGG AAGAGGTTGAGGTGGTGGAAGGCGTGGCTGGAGAGGAGGATCATCATGATGAACAGGAAGAGCACGGGGA AGAGAATGCTGAGGCTGAGGGACATCACGATGAGCATGATGAAGACGGAAGTGACATGGAGCTAGATTTG TTAGCAGCAGCTGAAACGGAAAGTGACAGTGAAAGTAACCATAGTAACCAAGATAATGCTAGTGGGCGCA GAAGTGTTGTCACCGCAGCCACTGCTGGCTCAGAAGCAGGAGCGAGCAGTGTTCCTGCCTTTTTTTCTGA AGATGACTCTCAGTCAAATGACTCAAGTGACTCTGACAGCAGTAGCAGTCAGAGTGATGACATAGAGCAG GAGACCTTTATGCTCGATGAGCCACTAGAAAGGACAACGAATAGCTCCCATGCAAATGGTGCTGCCCAGG CTCCTCGCTCCATGCAGTGGGCTGTCCGAAATCCGCAGCATCAGCGAGCAGCAAGCACAGCTCCGTCCAG CACATCTACGCCAGCAGCAAGTTCTGCAGGTTTGATTTATATTGATCCTTCAAATTTACGCCGGAGCGGC ACCATCAGTACAAGTGCTGCAGCGGCAGCAGCTGCTTTGGAAGCTAGCAATGCCAGCAGCTACTTAACAT CTGCAAGCAGTTTAGCCAGGGCTTACAGCATTGTCATCAGACAAATCTCGGACTTGATGGGCCTTATCCC TAAGTATAATCACCTAGTATACTCTCAGATTCCAGCAGCTGTGAAATTGACTTACCAAGATGCAGTCAAC TTACAGAACTATGTAGAAGAAAAGCTTATTCCTACGTGGAACTGGATGGTCAGTGTTATGGATTCTACTG AAGCTCAATTACGTTATGGTTCTGCACTAGCATCTGCTGGTGACCCAGGACACCCAAATCATCCCCTTCA TGCCTCTCAGAATTCAGCTCGAAGAGAGAGGATGACTGCACGGGAAGAGGCCAGTCTAAGAACGCTTGAA GGCAGACGACGTGCTACATTGCTGAGCGCCCGCCAGGGAATGATGTCTGCTCGAGGAGACTTTCTCAATT ATGCTCTGTCTTTAATGAGATCTCATAATGATGAGCATTCTGATGTTCTTCCAGTATTGGATGTGTGCTC ATTGAAACATGTGGCATATGTTTTCCAAGCCCTTATATACTGGATTAAAGCAATGAATCAGCAGACAACA TTAGACACTCCTCAACTGGAACGCAAAAGGACACGAGAACTTCTGGAACTGGGTATTGATAATGAAGATT CAGAACATGAAAATGATGATGACACCAGTCAAAGTGCTACTTTGAATGATAAGGATGACGACTCTCTTCC TGCAGAAACTGGCCAAAACCATCCATTTTTCCGACGTTCAGACTCTATGACATTCCTTGGATGTATACCT CCAAATCCATTTGAAGTGCCTTTGGCTGAAGCCATACCCTTGGCTGATCAGCCACATTTGTTGCAGCCAA ATGCTAGAAAGGAAGATCTTTTTGGTCGCCCAAGTCAGGGTCTTTATTCCTCATCTGCCGGAAGTGGGAA ATGCATAGTAGAGGTTACAATGGACAGAAATTGTCTCGAGGTTCTTCCAACTAAAATGTCTTATGCTGCC AATCTAAAAAATGTAATGAACATGCAAAACCGGCAGAAGAAAGAAGGGGAAGAACAGAGCCTGCTGGCCG AAGAAGCTGACAGTTCAAAGCCAGGTCCATCCGCTCCTGATGTTGCTGCCCAGTTGAAAAGCAGTTTACT AGCAGAGATAGGACTCACGGAAAGTGAAGGGCCCCCCCTTACATCTTTCAGGCCTCAGTGCAGTTTCATG GGCATGGTTATCTCCCATGACATGCTGCTGGGACGCTGGCGCCTGTCTTTAGAACTGTTCGGCAGAGTAT TCATGGAAGATGTTGGTGCAGAGCCTGGATCGATTTTAACTGAATTGGGTGGTTTTGAGGTAAAAGAATC AAAGTTCCGTAGAGAGATGGAAAAACTGAGAAACCAGCAATCAAGAGATTTATCACTAGAGGTCGATCGC GATCGGGATCTTCTCATTCAGCAGACTATGAGACAGCTTAACAACCATTTTGGCCGAAGATGTGCTACCA CGCCAATGGCTGTGCACAGAGTCAAAGTCACCTTTAAGGATGAACCAGGAGAGGGCAGTGGTGTAGCAAG AAGTTTCTATACAGCCATTGCACAAGCGTTTTTATCAAATGAAAAATTGCCAAATCTAGATTGTATCCAA AATGCCAACAAAGGTACTCATACAAGTTTAATGCAAAGATTGAGGAACAGAGGTGAAAGAGACCGGGAGA GAGAGAGAGAACGGGAAATGAGGAGGAGCAGTGGCTTGCGTGCAGGATCTCGAAGAGACAGGGATAGAGA CTTCAGGAGACAGCTCTCCATTGACACCAGGCCTTTCAGACCTGCTTCTGAGGGGAATCCTAGTGATGAC CCTGACCCTCTTCCAGCTCATCGGCAGGCACTTGGAGAGAGGCTTTACCCCCGAGTGCAAGCAATGCAGC CCGCATTTGCAAGTAAAATCACTGGCATGCTGTTGGAGTTATCCCCAGCTCAGCTGCTTCTCCTTCTAGC AAGTGAAGATTCTCTGAGGGCGAGAGTCGACGAGGCCATGGAACTCATTATTGCTCATGGACGGGAAAAT GGAGCTGACAGTATTTTGGACCTCGGATTATTAGACTCTTCAGAAAAGGTACAGGAAAACAGAAAGCGCC ATGGCTCTAGTAGAAGTGTAGTGGATATGGATTTAGAGGACACAGATGATGGTGATGACAACGCTCCCTT GTTTTACCAACCTGGGAAGAGAGGATTTTATACTCCGAGACCTGGCAAAAACACAGAAGCAAGGCTGAAT TGTTTCAGAAACATCGGCAGGATTCTTGGACTATGTCTGTTGCAAAATGAACTATGTCCTATCACATTGA ATAGGCATGTAATTAAAGTGTTGCTTGGTAGAAAAGTCAACTGGCATGACTTTGCTTTTTTCGACCCTGT AATGTATGAGAGTTTACGACAACTGATCCTTGCATCTCAGAGTTCAGATGCTGATGCTGTTTTCTCAGCA ATGGATTTGGCATTTGCAATTGACCTGTGTAAAGAAGAGGGTGGAGGACAGGTTGAACTTATTCCAAATG GTGTAAATATACCAGTCACTCCGCAGAATGTATATGAGTATGTAAGGAAATATGCAGAGCATAGAATGTT GGTGGTTGCAGAACAGCCATTGCACGCAATGAGAAAGGGTCTACTAGACGTGCTTCCAAAAAATTCCTTA GAAGACTTAACAGCTGAGGACTTCCGGCTCTTGGTGAACGGCTGTGGTGAAGTCAATGTGCAGATGCTGA TCAGTTTCACTTCTTTCAATGATGAGTCAGGAGAAAATGCTGAGAAGCTCCTCCAGTTCAAACGGTGGTT CTGGTCAATAGTAGAGAAAATGAGCATGACAGAACGGCAGGACCTGGTTTACTTCTGGACATCTAGCCCA TCATTGCCAGCCAGTGAAGAAGGTTTTCAGCCTATGCCCTCGATCACAATAAGACCACCAGATGACCAAC ATCTTCCTACTGCAAATACTTGTATTTCTCGACTTTATGTCCCACTCTATTCCTCTAAACAGATTCTCAA ACAAAAATTACTACTCGCCATTAAGACCAAGAATTTTGGTTTTGTGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001112721
Insert Size	8379 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001112721.2, NP_001106192.1
RefSeq Size	9773 bp
RefSeq ORF	8379 bp
Locus ID	70790
UniProt ID	Q80TP3
Gene Summary	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity). Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Plays an essential role in extraembryonic development. Regulates DNA topoisomerase II binding protein (TopBP1) for the DNA damage response.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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