Nipbl (NM_027707) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Idn3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225415 representing NM_027707 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGGGATATGCCCCATGTCCCCATAACTACGCTTGCGGGGATTGCTAGTCTTACGGACCTCCTGA ACCAGCTGCCTCTCCCATCTCCTTTACCTGCTACAACTACAAAGAGCCTCCTCTTTAATTCACGAATAGC AGAAGAGGTGAACTGCCTTTTGGCCTGTAGGGATGACAATTTGGTTTCACAGCTTGTCCATAGTCTCAAC CAGGTATCAACAGATCACATAGAACTGAAAGATAACCTTGGCAGTGATGATCCCGAAGGCGATATACCCG TCTTGTTGCAGGCCGTCCTGGCAAGGAGTCCGAACGTTTTCAGGGAGAAAAGCATGCAGAACAGATATGT ACAGAGTGGAATGATGATGTCTCAGTATAAACTTTCTCAGAATTCCATGCACAGTAGTCCTGCATCTTCC AATTACCAACAAACCACTATCTCACATAGCCCCTCCAGCCGGTTTGTGCCACCACAGACAAGCTCTGGGA ACAGATTTATGCCACAACAAAATAGCCCAGTGCCTAGTCCATATGCCCCACAGAGTCCTGCAGGATACAT GCCATATTCCCATCCTTCAAGTTACACAACACATCCGCAGATGCAGCAAGCATCGGTATCAAGTCCTATT GTTGCCGGTGGCTTGAGAAACATCCATGATAACAAAGTTTCTGGTCCATTATCTGGCAACTCAGCTAACC ATCATGCTGATAACCCTAGACATGGCTCAAGTGACGACTACCTACACATGGTGCACAGGCTAAGTAGTGA TGATGGCGATTCTTCAACAATGAGGAATGCTGCATCTTTCCCCTTAAGATCTCCTCAACCAGTGTGTTCT CCTGCTGGAAGTGACGGAACACCTAAAGGCTCAAGACCACCTTTAATTCTACAGTCTCAGTCTTTACCTT GTTCATCACCTCGAGATGTTCCTCCAGATATCTTACTAGATTCTCCAGAAAGAAAACAAAAAAAGCAAAA GAAAATTAAATTAGGCAAGGATGAAAAAGACCAGAACGAGAAAGCTGCAATGTATGATATAATTAGCTCT CCAACCAAGGACTCCACTAAACTTACATTAAGACTTTCTCGTGTAAGATCTTCAGATATGGACCAGCAAG ACGATATGCTATCTGGTATGGAAAATAGCAATGTTTCAGAAAATGATATTCCTTTTAATGTGCAGTATCC AGGACAGACTTCAAAAACACCCATTACTCCACAGGACGTAAACCGCCCACTTAATGCTGCTCAGTGTTTG TCGCAGCAAGAACAAACAGCATTCCTTCCAGCAAATCAAGTGCCTGTTTTACAACAGAACACTTCAGTTG CTACAAAACAACCACAGACTTCTGTAGTACAGAATCAGCAGCAGGTATCACAACAGGGGCCGATATATGA TGAAGTCGAATTGGACGCGTTGGCAGAAATTGAACGGATAGAGCGAGAATCAGCCATTGAAAGGGAGCGC TTCTCAAAGGAAGTTCAAGATAAGGATAAGCCTTTGAAAAAAAGAAAACAAGATTCTTACCCACAGGAGG CTGGGGGTGCTACAGGAGGTAATAGACCAGCTTCTCAGGAGACGGGTTCTACGGGAAATGGGTCAAGGCC AGCATTAATGGTTAGCATTGATCTTCATCAGGCAGGAAGAGTGGACTCTCAGGCTTCTATAACTCAGGAT TCAGACTCCATAAAAAAGCCTGAAGAAACCAAACAGTGTAATGATGCGCCCATTTCTGTTCTTCAGGAAG ATATTGTTGGAAGTCTTAAGTCTATACCAGAAAACCATCCTGAGACTCCTAAAAAAAAGTCTGATCCTGA GCTTTCAAAAAGCGAAATGAAACAAAATGAAAGTAGGTTGTCAGAATCTAAACCAAATGAAAATCAGCTG GGGGAGTCAAAATCAAATGAAAGTAAGTTAGAAACTAAAACTGAGACCCCAACAGAAGAACTTAAGCAGA ACGAGAACAAAACGACAGAGTCCAAACAGAGCGAGAGCGCTGTAGTTGAGCCTAAACAGAATGAAAATAG ACCGTGTGACACAAAACCAAATGACAACAAACAGAACAACACCAGGTCAGAAAACACAAAAGCAAGACCT GAAACCCCAAAGCAAAAAGCTGAAAGCCGGCCTGAAACCCCAAAGCAAAAGAGTGAAGGCCGGCCTGAAA CCCCAAAACAGAAGGGTGATGGACGACCTGAGACTCCAAAGCAAAAGAGTGAAGGCCGGCCTGAAACTCC AAAGCAAAAAGGTGAAGGCCGGCCTGAAACTCCAAAACATAGGCATGAAAATAGGAGGGATTCTGGAAAG CCATCAACAGAGAAGAAACCCGATGTGTCTAAGCATAAACAGGATATCAAATCTGACTCACCTCGATTAA AATCAGAAAGAGCTGAAGCCTTAAAGCAGAGACCTGATGGGCGATGGGAGTCACTAAGACGTGACCATGA TAGTAAACAGAAATCAGATGACAGGGGTGAGTCAGAGCGACATCGGGGTGATCAGTCAAGGGTTCGAAGA CCAGAAACATTGAGATCTTCTAGTAGAAATGACCATAGCACTAAATCAGATGGTTCAAAAACTGAAAAGC TAGAAAGAAAACACAGGCATGAATCAGGGGACTCACGGGACAGACCTTCTGGGGAACAAAAATCAAGACC AGACAGTCCTCGTGTAAAACAAGGAGATACTAATAAATCAAGACCTGGTTTTAAGTCACCAAATAGTAAA GATGACAAAAGGACAGAGGGTAACAGAAGTAAAGTAGACAGTAATAAAGCGCACACTGATAATAAGGCCG AATTTCCAAGCTATTTGTTGGGGGGGAGGTCTGGTGCATTGAAAAATTTCGTCATTCCAAAAATCAAGAG GGATAAAGATGGCAACATAACTCAGGAAACTAAGAAAATGGACATGAAAGGAGAGCAGAAAGACAAAGTA GAAAAAATGGGATTAGTTGAAGATCTAAACAAAGGAGCTAAGCCTGTAGTTGTTCTGCAAAAACTATCGC TGGATGATGTCCAGAAGCTAATTAAAGATAGAGAGGAGAAATCAAGAAGTTCCCTTAAGTCTATCAAGAA TAAGCCATCAAAGTCAAATAAAGGTAGTATAGATCAATCAGTGTTAAAAGAATTGCCCCCTGAGCTCCTG GCAGAAATTGAATCTACCATGCCACTTTGTGAACGTGTGAAAATGAACAAACGCAAGCGTAGCACAGTTA ATGAAAAGCCAAAATATGCTGAAATCAGTTCAGATGAAGATAATGATAGTGATGAAGCGTTTGAATCCTC TAGGAAACGGCATAAAAAAGATGACGATAAAGCTTGGGAATACGAAGAGCGCGACAGAAGAAGCTCTGGG GATCATAGGAGAAGTGGCCACTCCCATGACGGAAGGAGGAGCTCGGGTGGTGGTCGTTACCGAAACCGAA GTCCGTCCGATTCTGACATGGAAGATTACTCCCCTCCTCCCAGCCTTAGTGAGGTTGCTAGAAAAATGAA GAAAAAAGAAAAACAAAAGAAAAGGAAAGCTTACGAGCCAAAGCTAACGCCCGAAGAAATGATGGATTCT TCAACTTTTAAGAGATTTACAGCCTCAATAGAGAATATTTTGGATAATTTAGAAGATATGGATTTTACTG CATTTGGTGATGACGATGAGATTCCTCAGGAACTGCTTTTAGGAAAACATCAGCTTAATGAACTTGGGAG TGAATCTGCTAAAATTAAAGCCATGGGTATAATGGACAAGCTTTCAACTGACAAAACTGTGAAAGTCCTA AATATATTGGAGAAGAATATTCAGGATGGATCAAAGCTTTCCACTTTGTTAAATCATAATAATGATACTG AAGAAGAAGAAAGATTGTGGAGAGACCTAATTATGGAAAGAGTTACAAAGTCAGCAGATGCCTGTCTTAC AACTATCAACATTATGACATCTCCTAACATGCCAAAAGCTGTATACATTGAAGATGTAATTGAAAGGGTT ATACAGTACACTAAGTTTCATTTACAGAACACACTCTATCCCCAGTATGACCCTGTTTACAGAGTAGATC CACATGGAGGAGGGTTATTAAGTTCAAAGGCCAAACGGGCTAAATGTTCTACTCATAAGCAGAGAGTAAT TGTAATGCTTTACAACAAAGTTTGTGACATTGTTAGCAGTTTATCAGAATTGCTAGAGATACAGCTTCTT ACAGACACAACAATTCTTCAGGTTTCATCAATGGGAATAACACCCTTTTTTGTGGAAAATGTCAGTGAAC TACAATTGTGTGCCATTAAGTTAGTCACTGCAGTATTTTCAAGATATGAAAAGCATAGGCAGTTAATATT GGAAGAAATTTTTACTTCACTTGCAAGATTACCAACTAGCAAGAGAAGTTTAAGGAATTTCAGGTTAAAT AGTAGTGATGTGGATGGAGAACCTATGTATATTCAGATGGTTACAGCTCTAGTTTTACAACTTATTCAGT GCGTAGTTCACTTACCATCATCGGAGAAGGATCCTAATTCAGAAGAAGACTCAAACAAAAAAGTTGATCA GGATGTTGTCATTACGAACTCTTACGAAACAGCTATGAGAACAGCCCAAAACTTTCTCTCCATCTTTCTT AAAAAATGTGGTAGTAAACAAGGAGAAGAAGATTACAGACCGCTTTTTGAAAACTTTGTTCAAGACCTTC TCTCAACAGTCAATAAGCCCGAGTGGCCGGCTGCTGAACTACTCCTTAGCTTGTTAGGGAGGCTGTTGGT TCATCAGTTCAGTAACAAGTCAACAGAAATGGCTTTAAGAGTGGCATCTCTTGATTACCTTGGGACTGTT GCTGCGCGACTGAGAAAAGATGCTGTCACCAGCAAGATGGATCAAGGGTCTATAGAACGAATTTTAAAAC AGGTTTCTGGAGGAGAAGATGAGATCCAACAATTGCAAAAAGCATTGCTTGATTATCTGGATGAGAACAC TGAAACTGACCCTTCGCTAGTGTTTTCTCGTAAATTCTATATAGCCCAGTGGTTCCGTGACACAACTCTG GAAACAGAAAAAGCAATGAAATCACAAAAAGATGAAGAATCATCTGACGCAACACATCATGCAAAAGAAC TTGAGACAACTGGGCAGATCATGCATCGCGCTGAGAACCGGAAAAAGTTTCTTCGAAGCATTATCAAAAC CACACCTTCTCAGTTCAGCACATTAAAGATGAACTCTGACACTGTGGACTATGATGATGCGTGCTTGATT GTTCGATATTTGGCCTCTATGAGGCCGTTTGCCCAGAGCTTTGATATTTATTTGACACAGATACTACGAG TTCTTGGTGAAAATGCAATTGCCGTTCGAACAAAAGCCATGAAGTGTTTGTCTGAGGTTGTTGCTGTAGA CCCCAGTATTCTAGCAAGGCTTGACATGCAGCGCGGAGTTCATGGGCGACTAATGGATAACTCCACTAGT GTTCGAGAGGCAGCAGTGGAATTGCTAGGTCGCTTTGTCCTGTGTCGCCCTCAGCTTGCTGAGCAGTATT ATGACATGCTGATTGAAAGAATATTGGATACTGGGATCAGTGTCAGGAAAAGAGTAATAAAGATTTTAAG AGACATCTGTATTGAACAACCAACATTTCCAAAAATTACAGAAATGTGTGTAAAAATGATTCGTAGAGTC AATGATGAAGAGGGCATTAAGAAATTAGTAAATGAAACATTCCAAAAGCTCTGGTTTACTCCAACTCCAC ATAATGACAAAGAAGCAATGACAAGGAAGATTTTAAACATCACTGATGTGGTTGCAGCATGTAGAGATAC TGGATATGACTGGTTTGAGCAACTCCTTCAAAATTTGTTGAAGTCTGAAGAGGATTCCTCATATAAACCT GTGAAGAAAGCTTGTACTCAACTTGTTGATAACCTAGTTGAACATATTCTTAAATATGAGGAATCTCTAG CTGACTCAGACAATAAAGGTGTAAATTCTGGAAGATTGGTAGCTTGTATAACCACTTTGTTCTTATTCAG CAAAATAAGACCCCAACTCATGGTTAAACATGCCATGACTATGCAGCCGTACCTCACCACTAAGTGTAGT ACGCAAAATGATTTCATGGTTATCTGCAATGTTGCAAAAATCCTTGAGTTAGTTGTACCACTGATGGAAC ACCCAAGTGAAACTTTCCTTGCCACTATTGAGGAAGATCTGATGAAGCTGATCATCAAATACGGCATGAC TGTAGTACAACATTGTGTGAGCTGTCTTGGGGCTGTTGTAAATAAAGTGACCCAGAATTTTAAATTTGTG TGGGCCTGTTTCAATAGATACTATGGTGCCATTTCAAAATTAAAAAGTCAGCACCAAGAAGACCCAAATA ACACTTCACTTTTAACAAATAAACCAGCGCTTCTTAGGTCCCTCTTCACTGTTGGAGCACTTTGCCGCCA TTTTGATTTTGATCTGGAAGATTTTAAAGGCAACAGTAAGGTTAACATAAAGGATAAAGTACTTGAACTG TTGATGTATTTTACAAAACACTCAGATGAAGAAGTACAAACAAAAGCCATCATTGGTCTAGGATTTGCCT TTATACAGCATCCGAGTCTAATGTTTGAGCAAGAAGTGAAGAATCTGTATAATAGTATTTTATCTGATAA GAACTCCTCAGTGAATTTAAAAATACAAGTGTTAAAAAACCTCCAGACTTACCTACAAGAAGAAGACACG CGAATGCAACAAGCAGACAGAGACTGGAAGAAAGTTGCAAAACAGGAAGACCTGAAGGAAATGGGGGACG TCTCCTCGGGGATGAGCAGCTCCATCATGCAGCTGTACCTCAAGCAGGTGCTGGAGGCGTTCTTTCATAC CCAGTCAAGTGTGCGCCACTTCGCCCTGAATGTCATTGCTCTGACTCTGAACCAAGGCCTCATCCATCCG GTTCAGTGTGTTCCATACTTAATTGCCATGGGAACAGATCCAGAACCTGCTATGCGAAACAAGGCTGATC AGCAGCTTGTGGAGATAGACAAAAAATATGCTGGGTTCATCCATATGAAAGCCGTGGCTGGTATGAAGAT GTCTTACCAGGTCCAACAGGCGATCAACACGTGCCTAAAAGACCCTGTAAGGGGTTTCCGACAAGATGAA TCCTCCAGTGCTCTGTGCTCACATCTATACTCCATGATCCGCGGGAACCGCCAACACAGACGCGCCTTTC TTATTTCTTTACTCAACCTCTTTGATGACACAGCAAAAACAGAAGTGACTATGCTCTTGTACATAGCAGA CAATCTAGCCTGTTTTCCATACCAGACTCAGGAAGAGCCGCTGTTTATAATGCACCACATAGACATTACG CTCTCTGTTTCCGGTAGTAACCTATTGCAGTCATTCAAGGAGTCTATGGTAAAAGACAAAAGGAAAGAGA GGAAAACGTCCCCTGCTAAAGAGAACGAGTCCAGTGAGAGTGAAGAAGAAGTGTCCAGACCTCGGAAGTC TCGAAAGCGGGTAGATTCGGAGTCAGACTCAGACTCTGAAGATGATATAAATTCAGTGATGAAGTGTTTG CCGGAAAATTCAGCCCCTTTGATTGAATTCGCAAATGTTTCCCAGGGTATTTTATTACTTCTTATGTTAA AGCAGCACCTGAAGAATCTCTGTGGATTTTCTGATAGTAAAATTCAAAAATATTCTCCATCTGAATCTGC AAAAGTGTATGATAAAGCCATTAATCGGAAAACAGGGGTTCATTTTCATCCCAAACAAACACTGGACTTC CTGCGAAGTGACATGGCTAATTCCAAACTGACGGAGGATGTGAAGAGGAGCATCGTAAGGCAGTACTTAG ACTTTAAACTTCTCATGGAACATCTAGACCCTGATGAAGAAGAAGAGGAAGGAGAGGTGTCAGCTAGCAC CAATGCTCGGAACAAAGCAATTACCTCGCTGCTTGGAGGAGGCAGCCCTAAAAATAACACAGCAGCAGAC ACAGAAGATGAGGAGAGTGATGGGGAGGATAGAGGAGGAGGCACTTCAGGGTCATTGAGAAGGTCAAAAC GAAATTCAGACTCTACGGAGTTGGCAGCACAGATGAATGAAAGTGTTGACGTCATGGATGTCATCGCTAT TTGCTGTCCAAAGTACAAAGACCGACCACAGATTGCAAGAGTAGTACAGAGAACCAGCAGTGGCGTCAGT GTCCAGTGGATGGCAGGCTCCTACAGCGGCTCCTGGACGGAGGCCAAGCGCCGCGATGGCCGCAAACTGG TGCCTTGGGTAGACACTATTAAAGAGTCAGACATTATTTACAAAAAAATTGCTCTAACGAGTGCTAATAA GCTGACTAATAAAGTTGTTCAGACTTTACGATCCCTGTATGCCGCCAAGGATGGGACTTCCAGCTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_027707
Insert Size	8397 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_027707.3, NP_081983.2
RefSeq Size	10216 bp
RefSeq ORF	8397 bp
Locus ID	71175
UniProt ID	Q6KCD5
Gene Summary	Plays an important role in the loading of the cohesin complex on to DNA (PubMed:29094699). Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin. Plays a role in cohesin loading at sites of DNA damage. Its recruitement to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitement to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner (By similarity). Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others (PubMed:28041881).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (a) encodes the longer isoform (a).

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