Col7a1 (NM_007738) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW209154
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225435 representing NM_007738 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGCGACTCCTGGTCGCTGCGCTCTGCGCTGCAGAGATCCTGATGGGAGCGCCGGAAGTGTGGG CCCAGCCCAGAGATAGAGTGACCTGCACACGCCTTTATGCTGCTGACATCGTGTTCTTACTCGATGGCTC GTCGTCCATTGGCCGCAGCAACTTTCGAGAAGTGCGAGGATTCCTGGAGGGGCTGGTGCTGCCTTTCTCA GGAGCAGCCAGTGCACAGGGCGTCCGGTTTGCCACAGTGCAGTACAGTGATGATCCACAGACAGAATTTG GTCTGGATACACTTGGCTCTGGGAGTGACACCATCCGAGCCATCCGTGAGCTCAGCTATAAAGGTGGTAA CACTCGCACGGGGGCCGCTCTTCACCACGTTTCTGACCGTGTCTTCCTGCCTCGTCTAACACGTCCTGGT GTCCCCAAGGTCTGCATCCTGATCACAGATGGGAAATCCCAAGATTTAGTGGACACAGCTGCCCAAAAGC TGAAGGGGCAGGGAGTCAAGCTGTTTGCTGTGGGAATCAAGAATGCCGACCCTGAGGAACTGAAGCGTGT GGCCTCACAGCCAACCAGCGATTTCTTCTTCTTCGTCAATGACTTCAGCATCTTGAGGACCCTGTTGCCT CTCATTTCCAGGAGGGTGTGCACAACTGCTGGTGGTGTGCCTGTGACCCTGCCTTCTGATGACACTCCGT CTGGGCCCCGGGACCTGGTGCTCTCTGAGCCAAGTAGCCAATCCTTGCGAGTACAATGGACAGCAGCCAG TGGCCCTGTGACTGGCTACAAGGTTCAGTACACACCTCTGACAGGGCTAGGGCAGCCACTGCCAAGTGAG CGACAGGAGGTGAACATCCCGGCTGGTGAGACCAGCACGCGTCTCCAGGGTCTCCGGCCACTAACGGACT ACCAAGTGACTGTGGTTGCCCTCTATGCCAACAGCATCGGGGAGGCCGTGAGTGGGACAGCTCGGACCAC TGCCAAGGAAGGGCTAGAATTGAGCCTCCAGAACATCACATCGCACAGCCTCCTGGTGGCCTGGAGGAGG GTACCAGGTGCTAATGGCTACCGTGTAACATGGCGGGACCTCAGTGGTGGGCCAACCCAGCAGCAGGACC TGAGCCCTGGACAGGGGTCAGTGTTTCTAGATCACTTGGAGCCTGGCACGGACTATGAGGTGACCGTGAG CGCCCTATTTGGCCACAGCGTGGGGCCTGCCGCTTCTCTGACTGCCCGCACCGCATCTTCTGTTGAGCAA ACCCTGCATCCCATCATCTTGAGCCCGACATCCATCCTCCTTTCCTGGAACTTGGTGCCTGAGGCCCGCG GCTACCGCTTGGAGTGGCGTCGTGAGAGTGGCTTGGAAACACCACAGAAAGTGGAACTGCCCCCTGATGT GACCCGCCATCAGCTGGATGGTTTGCAGCCAGGCACTGAGTACCGTCTCACGCTGTATACTCTGCTGGAG GGCCGTGAGGTGGCCACTCCTGCAACCGTGGTTCCTACAGGGCTAGAGCAGCTGGTGAGCCCGGTGATGA ATTTACAAGCCATAGAACTACCTGGGCAGCGGGTGCGAGTGTCTTGGAACCCAGTCCCTGGGGCCACTGA GTACCGTTTCACTGTACGCACCACCCAGGGGGTCGAGCGGACTCTATTGCTTCCTGGGAGCCAGACAACC TTTGACTTAGATGATGTTCGAGCTGGGCTTAGCTACACGGTGCGGGTGTCTGCTCGAGTGGGTGCTCAGG AGGGTGATGCCAGCATCCTAACGATCCACAGAGATCCAGAAGCCCCACTTGTTGTCCCAGGACTGCGGGT TGTCGCATCAGATGCAACAAGAATTAGGGTGGCCTGGGGACTTGTCCCTGGAGCCAGTGGATTTCGGATC AGCTGGAGGACAGGCAGTGGTCCAGAGTCCAGCCGGACGCTGACCCCAGACTCCACTGTCACGGACATCC TGGGGTTGCAGCCTAGCACCTCTTATCAAGTCGCTGTCTCAGCACTTAGAGGGAGAGAAGAGGGGCCCCC TGTGGTCATCGTGGCTAGAACTGATCCACTGGGCCCAGTGAGGAGAGTCCACTTGACTCAGGCTGGCAGC TCATCTGTCAGCATCACCTGGACTGGGGTTCCCGGTGCCACAGGATACAGGGTTTCCTGGCACTCAGGCC ATGGTCCAGAGAAATCTCTGTTGGTTTCTGGAGATGCCACGGTGGCTGAAATAGATGGGCTGGAGCCGGA CACAGAGTATATAGTGCGTGTGAGAACCCACGTAGCTGGTGTGGATGGAGCCCCCGCCTCTGTGGTTGTG AGGACTGCTCCTGAGCCTGTGGGCAGTGTGTCGAAACTGCAAATCCTTAATGCTTCCAGTGATGTTCTAC GGGTCACTTGGGTAGGAGTCCCTGGGGCCACATCCTATAAACTGGCGTGGGGACGTAGTGAAGGTGGCCC TATGAAACACCGGATACTCCCAGGAAACAAAGAGTCTGCAGAGATACGGGATCTCGAAGGAGGAGTCAGC TACTCCGTGAGAGTGACGGCGCTTGTTGGAGACCGAGAGGGTGCACCGGTGTCCATTGTCATCACCACAC CGCCTGCGACCCCAGCACTTCTGGAGACTCTTCAAGTTGTGCAGAGCGGGGAGCACTCCTTGAGGCTGCG ATGGGAACCAGTACCCGGAGCACCAGGCTTCCGCCTGCACTGGCAACCTGAGGGTGGCCAGGAACAGTCC CTGACCTTGGGACCTGAGTCTAACAGCTACAACCTGGTTGGTCTGGAGCCAGCAACAAAGTACCAAGTAT GGCTGACTGTCCTGGGCCAGACTGGAGAAGGTCCCCCTAGGAAGGTGACTGCATACACTGAGCCCTCTCA CATCCCGAGCACTGAACTACGCGTGGTGGATACCTCTATTGACTCAGTGACTTTGACCTGGACCCCAGTG TCTGGGGCATCCAGTTATATCTTGTCCTGGCGGCCGCTCAGAGGAACTGGCCAGGAAGTGCCGAGGGCCC CACAGACACTGCCAGGGACCTCAAGCTCCCACAGAGTGACAGGGCTGGAACCTGGTATTTCCTATGTTTT CTCTCTGACACCTATCCAGAGCGGTGTACGAGGTTCTGAGATCTCTGTCACACAAACACCAGCGTGTTCC CATGGCCCAGTGGACGTGGTGTTCCTCTTGCATGCCACTCGAGACAATGCTCACAACGCAGAGGCTGTGA GGAGGGTCCTGGAAAGGCTGGTGTCTGCACTTGGGCCTCTTGGTCCACAGGCTGCTCAGGTCGGCCTGCT GACTTACAGCCACCGGCCCTCCCCACTGTTCCCACTGAATAGTTCCCATGATCTTGGCATCATCTTGCGA AAGATCCGTGACATCCCCTACGTGGACCCAAGTGGTAACAACCTCGGCACAGCCGTGACCACAGCTCACA GATACCTCTTAGCATCCAATGCTCCTGGTCGCCGTCAGCAAGTGCCAGGCGTGATGGTCCTGTTAGTGGA TGAGCCTCTGAGAGGGGACATACTCAGCCCCATTCGTGAGGCCCAGACTTCTGGGCTTAAGGTGATGGCA CTGAGTTTGGTGGGAGCTGACCCAGAGCAGCTGCGTCGCTTGGCACCAGGCACGGACCCCATCCAGAACT TCTTTGCTGTGGATAATGGCCCAGGCTTGGACAGAGCAGTCAGTGATCTGGCTGTAGCCCTGTGTCAGGC AGCTGTGACCATAGAGCCGCAAACAGGGCCTTGTGCTGTGCACTGTCCAAAGGGCCAGAAGGGGGAGCCT GGAGTAACGGGACTGCAAGGACAGGCTGGACCTCCTGGCCCCCCTGGTCTCCCGGGCAGGACCGGTGCTC CAGGCCCGCAAGGTCCTCCTGGAAGTACCCAGGCAAAAGGCGAGAGGGGCTTTCCTGGCCCAGAAGGGCC TCCAGGCAGTCCTGGCCTCCCTGGAGTTCCTGGGTCTCCTGGCATTAAGGGCTCCACGGGGCGGCCTGGC CCTCGTGGGGAACAGGGAGAACGAGGGCCTCAAGGTCCAAAGGGGGAGCCGGGTGAGCCTGGGCAAATCA CTGGCGGTGGAGGGCCAGGGTTTCCTGGAAAGAAAGGGGACCCTGGACCTTCGGGGCCCCCTGGATCTCG TGGCCCTGTGGGGGACCCAGGACCCCGTGGCCCCCCAGGGCTTCCTGGAATATCAGTGAAGGGTGACAAA GGTGATCGTGGGGAACGGGGTCCCCCGGGACCAGGCATTGGTGCCAGTGAACAGGGTGACCCTGGACTTC CGGGGCTTCCTGGAAGCCCTGGACCCCAAGGCCCAGCTGGCCGACCTGGAGAGAAGGGAGAAAAGGGTGA CTGTGAGGATGGAGGTCCAGGCCTCCCAGGACAGCCTGGGCCCCCAGGGGAGCCGGGCCTGCGGGGAGCT CCTGGAATGACTGGGCCCAAAGGTGATCGGGGACTGACGGGGACCCCGGGCGAGCCTGGAGTAAAGGGTG AACGGGGACATCCTGGTCCCGTGGGACCCCAGGGGCTCCCCGGGGCTGCTGGACACCCTGGAGTGGAGGG TCCTGAGGGGCCACCAGGACCCACTGGCCGCCGAGGAGAAAAGGGAGAACCTGGTCGCCCTGGGGATCCT GCAGTGGGTCCTGGTGGTGCTGGAGCCAAAGGAGAAAAGGGAGAGGCTGGGCTCCCTGGGCCCAGAGGAG CTTCTGGAAGCAAAGGAGAGCAGGGCGCACCTGGATTGGCTCTTCCTGGAGATCCTGGCCCCAAAGGAGA CCCTGGAGACAGGGGTCCCATTGGCCTCACTGGAAGGGCAGGACCCACAGGTGACTCAGGGCCTCCTGGA GAGAAAGGAGAGCCTGGTCGACCTGGTTCCCCAGGACCTGTTGGCCCCCGAGGAAGAGATGGTGAAGCTG GAGAGAAAGGTGACGAGGGGATCCCGGGTGAGCCAGGTTTGCCTGGAAAAGCAGGTGAACGTGGCCTCCG GGGGGCACCTGGGCCTCGGGGGCCTGTGGGTGAGAAGGGGGACCAGGGAGATCCTGGAGAAGACGGACGG AATGGCAGCCCTGGATCATCTGGACCCAAGGGTGACCGTGGGGAGCCTGGTCCCCCAGGTCCCCCTGGAC GGCTGGTGGATGCAGGCATTGAATCCAGAGACAAGGGAGAGCCTGGACAAGAAGGTCCCCGAGGACCCAA GGGTGACCCTGGGCCCCCTGGAGTCTCTGGAGAAAGGGGCATTGATGGGCTTCGGGGACCCCCAGGCCCA CAGGGAGACCCCGGTGTTCGAGGCCCAGCAGGAGACAAGGGTGATCGGGGACCCCCAGGGCTGGATGGCC GGAGTGGGCTGGATGGGAAACCCGGAGCCCCTGGCCCCCCAGGGCTACATGGTGCTTCAGGCAAAGCTGG GGACCCGGGGAGAGATGGACTTCCAGGCCTTCGAGGAGAACATGGCCCCCCTGGTCCCCCTGGCCCTCCT GGAGTTCCGGGAAAGGCAGGCGATGATGGCAAACCAGGCCTGAATGGGAAAAACGGAGACCCTGGAGACC CTGGAGAAGATGGAAGGAAGGGAGAAAAGGGAGATTCGGGTGCCCCTGGAAGAGAAGGCCCTGATGGCCC CAAGGGTGAACGTGGAGCTCCTGGCAATCCTGGACTCCAGGGTCCTCCGGGTCTCCCAGGGCAGGTCGGT CCTCCCGGCCAGGGTTTCCCTGGTGTCCCAGGAATTACGGGTCCTAAGGGTGACCGTGGAGAGACTGGAT CCAAAGGGGAACAGGGCCTTCCTGGAGAACGTGGCCTACGAGGAGAACCTGGGAGCTTGCCAAACGCGGA GCGTTTGCTGGAAACTGCTGGCATCAAGGTGTCGGCCCTGCGGGAGATCGTGGACACCTGGGATGAGAGT TCTGGCAGCTTCCTACCGGTGCCTGAGCGGAGACCTGGCCCCAAGGGGGACCCTGGTGACCGAGGCCCTC CAGGCAAGGAGGGCCTCATCGGCTTTCCTGGAGAACGTGGACTGAAGGGAGAGCGTGGAGACCCTGGCCC TCAGGGGCCTCCTGGCCTGGCCCTGGGTGAGAGGGGCCCACCTGGCCCACCCGGCCTTGCGGGGGAACCC GGAAAGCCTGGCATTCCTGGACTCCCAGGCCGGGCTGGTGGTTCAGGGGAAGCAGGAAGGCCAGGAGAGA GGGGAGAAAGGGGAGAGAAAGGAGAACGCGGGGATCAGGGCAGAGATGGCCTTCCCGGCCTCCCTGGACC ACCTGGCCCTCCTGGCCCCAAGGTGGCCATTGAAGAGCCAGGCCCTGGACTGGCTAGAGAGCAAGGACCT CCTGGACTCAAGGGTGCCAAGGGGGAGCCAGGCAGTGATGGTGACCCTGGACCTAAAGGAGACAGGGGTG TACCAGGCATCAAAGGCGATGTGGGAGAGCCTGGAAAGAGGGGTCATGATGGCAACCCAGGTCTCCCGGG AGAGCGTGGTGTGGCTGGGCCTGAAGGGAAGCCGGGTCTTCAGGGCCCAAGGGGGACCCCTGGCCCAGTG GGTAGCCATGGAGACCCTGGACCACCTGGTGCCCCGGGTCTTGCTGGCCCTGCGGGACCCCAGGGGCCTT CTGGCCTGAAGGGGGAGCCTGGAGAGACAGGACCTCCAGGACGGGGTCTGCCTGGACCTGTTGGAGCTGT GGGACTCCCTGGCCCTCCTGGCCCTTCCGGCCTCGTGGGTCCACAAGGCTCTCCAGGTTTGCCCGGACAA GTGGGGGAGACAGGGAAGCCAGGACCTCCAGGCCGTGACGGTAGCAGTGGAAAGGACGGAGATCGGGGAA GTCCTGGTGTGCCGGGGTCACCAGGTCTGCCCGGCCCTGTTGGACCTAAAGGAGAACCTGGGCCTGTGGG GGCTCCTGGACAGGTGGTGGTCGGGCCCCCTGGAGCAAAAGGCGAGAAGGGAGCCCCGGGAGACCTTGCT GGAGCCCTGCTGGGTGAGCCGGGAGCCAAAGGTGACCGAGGACTGCCTGGACCACGGGGTGAAAAGGGTG AAGCTGGCCGTGCAGGAGGACCTGGGGACCCTGGGGAAGATGGTCAAAAAGGGGCTCCAGGACTCAAAGG TCTTAAGGGTGAGCCAGGCATTGGGGTTCAGGGCCCCCCTGGCCCAAGTGGTCCTCCAGGTATGAAGGGA GACTTGGGCCCCCCTGGTGCCCCCGGGGCTCCTGGTGTTGTTGGGTTCCCTGGTCAGACAGGACCTCGAG GAGAGACGGGCCAGCCAGGGCCTGTTGGAGAGAGGGGTCTGGCAGGCCCTCCAGGGAGAGAAGGTGCCCC AGGTCCCTTGGGACCACCTGGACCACCAGGGTCAGCGGGAGCACCTGGCGCCTCTGGACTCAAAGGAGAC AAGGGAGACCCGGGAGCAGGACTGCCGGGGCCCCGAGGTGAGCGTGGTGAGCCAGGTGTCCGGGGTGAAG ATGGCCACCCTGGCCAGGAAGGACCTCGTGGACTTGTGGGGCCCCCTGGCAGCCGGGGAGAGCAAGGAGA GAAGGGCGCTGCTGGCGCTGCAGGGCTCAAGGGTGACAAGGGTGACTCAGCTGTGATTGAGGGACCTCCC GGGCCACGAGGTGCCAAAGGGGATATGGGTGAACGAGGGCCTCGGGGCATAGATGGTGACAAAGGACCTC GGGGAGAAAGTGGGAATCCTGGTGACAAGGGTTCCAAAGGAGAGCCAGGTGACAAAGGCTCAGCTGGATC GATTGGAGTTCGAGGACTCACAGGACCCAAGGGAGAGCCAGGTGCTGCAGGGATCCCCGGTGAGCCGGGA GCCCCAGGAAAGGATGGAATCCCTGGTTTCCGAGGAGACAAAGGAGATATTGGCTTCATGGGTCCCAGGG GCCTCAAGGGTGAAAAAGGAATAAAGGGAACCTGTGGCCGTGACGGAGAGAGAGGAGATAAGGGAGAAGC TGGTTTTCCTGGTCGCCCTGGGTTGGCAGGAAAGAAAGGAGACATGGGGGAGCCTGGTCTGCCGGGTCAG TCTGGGGCTCCTGGGAAAGAAGGCCTGATCGGTCCCAAGGGTGACAGAGGCTTTGATGGGCAGTCAGGAC CCAAGGGTGACCAAGGCGAGAAAGGGGAGAGGGGGCCTCCAGGAGTTGGGGGCTTCCCAGGTCCCAGAGG AAACGATGGCTCTAGTGGTCCCCCAGGGCCACCTGGTGGTGTTGGTCCCAAGGGACCCGAAGGGCTTCAG GGTCAGAAGGGTGAGCGAGGTCCCCCTGGAGAGAGCGTGGTGGGGGCTCCTGGTGCTCCTGGCACCCCTG GAGAGAGAGGAGAGCAGGGACGACCAGGACCTGCTGGCCCCCGTGGGGAGAAGGGAGAAGCTGCGCTGAC TGAGGATGACATTCGGGACTTTGTGCGTCAGGAGATGAGTCAGCACTGTGCCTGCCAGGGCCAGTTTATT GCGTCTGGATCACGACCCCTCCCCGGTTATGCTGCGGATACAGCTGGTTCCCAGCTTCACCATGTTCCTG TGCTCCGGGTCTCTCATGTTGAGGAGGAAGGCCAGGTGCCCCCTGAAGATGACGATGACTTCTCTGAGTA CTCTGTGTATTCTGTGGAGGACTACCAGGAGCCTGAAGTTCCATGGGATGGTGAAGCCGAGATTAAGGGC TGGGACCAGAGAGGTTCAGATCTCTGCTCCCTACCCTTGGATGAGGGCTCCTGTACTGCCTATACCCTGC GCTGGTACCATCGAGCTGTGCCTGGTGGCACAGCCTGTCATCCCTTTGTCTATGGTGGCTGCGGAGGGAA TGCCAATCGTTTTGGAACCCGTGAGGCGTGTGAGCGCCGCTGCCCACCCCAAGGGGTCCACAGCCAGAAA ACAGGTGCTGCCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_007738
Insert Size	8835 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007738.3, NP_031764.2
RefSeq Size	9222 bp
RefSeq ORF	8835 bp
Locus ID	12836
UniProt ID	Q63870
Gene Summary	Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.[UniProtKB/Swiss-Prot Function]

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