Atm (NM_007499) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI256621; C030026E19Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225456 representing NM_007499 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCTAGCACTCAATGATCTGCTCATTTGCTGCCGGCAGTTAGAGCATGACAGAGCTACAGAAAGAA GGAAAGAAGTGGATAAATTTAAGCGCCTGATTCAGGATCCTGAAACAGTTCAACATTTAGATAGGCATTC TGATTCCAAACAAGGAAAATATCTGAATTGGGATGCTGTTTTCAGGTTTTTACAGAAGTACATTCAAAAA GAAATGGAAAGTCTGAGAACAGCAAAATCAAATGTATCAGCCACCACACAGAGCTCCAGACAGAAGAAGA TGCAAGAGATCAGCAGTTTGGTCAGATACTTCATCAAATGTGCAAACAAAAGAGCACCCAGGCTAAAATG TCAAGACCTCTTGAATTATGTCATGGATACAGTGAAAGACTCATCTAATGGTCTAACGTATGGAGCTGAC TGTAGCAACATACTACTCAAAGACATTCTTTCTGTGAGAAAATACTGGTGTGAAGTATCTCAGCAACAGT GGCTAGAATTGTTTTCACTGTACTTCAGGCTGTATCTCAAGCCATCACAGGACATTAATAGAGTTTTAGT GGCTAGAATAATTCATGCTGTCACCAGAGGATGCTGTTCACAGACTGATGGATTACCTTCAAAGTTTTTA GATCTTTTTTCCAAGGCTATTCAGTATGCCAGACAAGAAAAGAGCTCTCCTGGCTTAAGTCACATCTTAG CAGCCCTTAACATTTTCCTCAAGTCTCTGGCTGTCAACTTCCGAAAACGGGTGTGTGAAGCAGGAGATGA AATTCTTCCTACCTTACTATATATTTGGACTCAACATAGACTTAATGATTCTTTAAAAGAAGTAATTATT GAACTAATTCAACTGCAGATTTATATCCATCATCCACAAGGAGCCAGAGCTCCTGAAGAAGGTGCTTATG AATCCATGAAATGGAAAAGTATCTTGTACAACTTATATGACTTGCTAGTGAATGAGATAAGTCATATAGG AAGCCGAGGGAAATATTCCTCAGGATCTCGTAATATTGCTGTCAAGGAAAATCTGATTGACCTGATGGCA GATATCTGTTACCAGCTTTTTGATGCAGATACCAGATCCGTGGAGATTTCTCAATCTTATGTGACACAAA GGGAATCCACTGATTACAGTGTACCTTGCAAAAGAAGGAAAATAGACGTAGGCTGGGAAGTGATAAAAGA TTATCTTCAGAAGTCACAGAGTGATTTTGATCTCGTGCCTTGGCTACAGATTACAACCCGATTAATATCA AAATATCCTTCCAGTTTACCTAACTGTGAGCTGTCTCCATTAATACTGATACTGTACCAGCTTCTGCCTC AACAGCGACGTGGAGAACGCATCCCATATGTGTTACGATGCCTTAAGGAAGTTGCCTTATGTCAAGGCAA GAAATCAAACCTGGAAAGCTCTCAGAAGTCAGATTTATTGAAACTATGGATCAAAATTTGGTCTATTACC TTTCGTGGTATAAGTTCTGGACAAACACAAACTGAAAACTTTGGTTTACTTGAGGCCATCATTCAAGGTA GTTTAGTTGAACTTGACAGAGAATTCTGGAAGTTATTTACTGGCTCAGCCTGTAAACCTTCTAGTCCTTC AGTATGCTGCTTGACTTTGGCACTTAGCATCTGTGTAGTTCCAGATGCAATAAAAATGGGAACAGAACAA AGTGTGTGTGAAGCAAATAGAAGTTTTTCTGTAAAGGAGTCAATAATGAGGTGGCTCTTATTCTACCAGT TAGAGGATGACTTAGAAGACAGCACAGAGCTGCCTCCAATTCTTCAGAGTAATTTTCCTCATCTTGTAGT CGAAAAAATTCTTGTAAGTCTCACTATGAAAAACTCAAAAGCTGCAATGAAGTTTTTTCAAAGTGTGCCA GAATGTGAACAACACTGCGAAGATAAAGAAGAGCCTTCATTTTCAGAAGTAGAAGAACTGTTTCTTCAGA CTACTTTTGACAAGATGGATTTTTTAACTACTGTCAAAGAGTATGCTGTAGAAAAATTTCAGTCTAGTGT TGGCTTCTCTGTCCAGCAAAATCTCAAGGAATCATTGGATCACTATCTTCTGGGATTATCAGAACAGCTT TTAAGTAATTACTCTTCTGAGATTACAAGTTCTGAAACCCTTGTCCGGTGTTCAAGTCTTTTGGTGGGTG TTCTTGGCTGCTATTGTTACATGGGTATAATAACTGAAGACGAAGCCCATAAATCAGAATTATTCCAGAA AGCCAAGTCTCTGATGCAATGTGCAGGAGAAAGTATCTCTCTGTTTAAAAATAAAACAAATGAGGAATCA AGAATTGGTTCATTGAGAAATGTGATGCATCTGTGTACAAGTTGCTTGTGTATACATACCAAGCATACGC CAAACAAGATTGCCTCTGGCTTTTTCCTACGATTATTAACATCAAAGCTTATGAATGACATTGCAGATAT TTGTAAAAGTTTAGCATCCTGTACGAAAAAGCCATTGGATCACGGAGTACATCCAGGGGAAGATGATGAA GATGGTGGTGGTTGTGACAGTCTGATGGAGGCAGAGGGTCCATCGTCCACTGGTCTTTCTACTGCTTACC CCGCTAGTTCTGTGAGCGATGCAAATGATTATGGAGAGAACCAGAATGCTGTTGGTGCCATGAGTCCTTT AGCTGCCGACTACCTGTCCAAACAAGATCATCTTCTCTTAGACATGCTCAGGTTCTTAGGCCGATCTGTA ACTGCATCTCAGAGCCATACTGTGTCGTTTAGAGGAGCTGACATTAGAAGAAAATTGTTACTGTTGCTTG ATTCTAGCATACTCGATCTCATGAAGCCCCTCCACCTGCATATGTACTTAGTGCTCCTGAAGGATCTCCC TGGAAACGAGCACTCATTGCCAATGGAAGATGTTGTTGAACTTCTGCAACCATTATCCCTTGTGTGTTCT CTGCACCGACGTGACCAAGATGTCTGTAAAACGATTCTAAGCAATGTCCTTCATATAGTGACAAACCTAG GCCAGGGCAGTGTGGACATGGAGAGCACACGGATTGCTCAAGGACACTTCCTGACAGTGATGGGAGCATT TTGGCATTTGACAAAGGAAAAGAAATGTGTATTCTCTGTAAGAATGGCATTAGTAAAGTGTCTTCAAACA TTGCTTGAGGCTGATCCATATTCCGAATGGGCAATTCTTAATGTAAAAGGACAAGACTTTCCTGTAAATG AAGCTTTTTCACAATTTCTTGCTGACGATCATCATCAAGTTCGGATGTTGGCTGCAGGGTCAGTCAACAG ATTATTTCAGGATATGAGACAAGGCGATTTCTCCAGAAGCTTGAAAGCACTCCCTCTGAAGTTTCAGCAG ACATCTTTTAACAATGCATACACGACAGCAGAGGCGGGGATCAGAGGACTGTTATGTGATTCTCAGAACC CTGATCTGCTGGATGAGATCTATAACAGAAAATCTGTACTACTGATGATGATAGCTGTGGTCTTGCACTG TAGCCCAGTCTGTGAAAAGCAGGCTTTGTTTGCTTTATGCAAGTCTGTGAAGGAAAACAGACTAGAACCT CATCTTGTGAAAAAGGTTTTAGAGAAAGTCTCCGAATCGTTTGGATGTAGAAGTTTAGAAGACTTCATGA TTTCTCACCTAGACTACCTGGTTTTGGAATGGCTGAACCTTCAAGATACTGAATATAGCTTATCTTCTTT TCCTTTTATGTTATTAAACTACACAAGCATTGAGGATTTCTATCGGTCTTGTTACAAGATTTTGATCCCA CATTTGGTAATCAGAAGCCATTTTGATGAGGTGAAGTCCATTGCTAATCAGATTCAAAAGTGCTGGAAAA GCCTGTTGGTAGATTGCTTTCCGAAGATTCTTGTGCACATCCTTCCTTACTTTGCCTACGAGGGCACGAG AGACAGCTACGTGTCACAGAAAAGAGAGACTGCTACCAAGGTCTACGATACTCTTAAAGGGGAAGACTTC CTAGGAAAACAGATTGACCAAGTATTCATTAGTAATTTGCCAGAGATTGTGGTGGAGTTGCTGATGACAT TGCATGAGACAGCTGACTCGGCTGACTCGGACGCCAGTCAAAGCGCCACCGCCTTGTGTGATTTTTCAGG GGATTTGGATCCTGCCCCCAACCCGCCATATTTCCCCTCACATGTCATTCAGGCAACGTTTGCTTACATC AGCAACTGTCATAAAACCAAGTTTAAAAGCATTCTAGAAATTCTTTCTAAAATCCCCGATTCCTATCAGA AAATACTTCTGGCCATTTGTGAACAAGCAGCTGAGACAAATAATGTCTTTAAAAAGCACAGAATTCTTAA AATATATCACCTGTTTGTTAGTTTATTACTGAAAGATATACAGAGTGGCCTGGGAGGGGCTTGGGCCTTT GTCCTTCGCGATGTTATTTATACTCTGATTCACTACATCAACAAAAGGTCTTCTCATTTCACAGATGTGT CGTTGCGTAGCTTTTCCCTTTGCTGTGACCTATTAAGTCGAGTTTGTCATACAGCTGTAACTCAATGTAA GGATGCTCTAGAAAGCCATCTTCACGTTATCGTTGGCACACTTATTCCCCTTGTGGATTATCAGGAAGTT CAAGAACAGGTATTGGACCTGTTGAAGTACTTAGTGATAGATAACAAAGACAATAAAAACCTCTCTGTCA CAATTAAGCTTTTGGATCCCTTTCCTGACCATGTTATTTTTAAGGACTTGCGTCTTACTCAACAGAAAAT CAAATATAGTGGAGGACCTTTTTCACTCTTAGAGGAAATAAACCATTTTCTCTCAGTAAGTGCTTACAAT CCACTTCCGCTGACCAGGCTTGAAGGACTGAAGGATCTTCGAAGACAACTGGAGCAACATAAAGATCAGA TGCTAGATCTTCTGAGAGCGTCTCAAGATAACCCACAAGATGGCATTGTGGTGAAGCTAGTTGTCAGCTT GTTGCAGTTATCCAAGATGGCAGTGAACCAGACTGGTGAAAGAGAAGTTTTAGAGGCTGTCGGAAGGTGT TTGGGAGAAATAGGTCCTCTGGATTTCTCCACCATAGCTGTCCAGCATAACAAAGATGTGTCCTATACCA AAGCCTACGGGTTACCTGAAGACAGAGAACTTCAGTGGACCTTGATAATGCTGACTGCCCTCAACAATAC CCTGGTAGAGGACAGTGTCAAAATTCGATCTGCTGCTGCTACCTGTTTGAAAAACATTTTGGCTACAAAG ATTGGACATATTTTCTGGGAGAATTATAAGACATCAGCGGATCCAATGCTGACCTATCTACAACCTTTTA GAACATCGAGGAAAAAGTTTTTAGAAGTGCCCCGATCTGTTAAAGAAGATGTTTTAGAAGGCCTGGATGC TGTGAATCTGTGGGTTCCTCAAAGTGAAAGTCATGACATTTGGATAAAGACACTGACGTGTGCCTTTCTG GACAGTGGAGGCATAAACAGTGAAATTCTCCAGTTATTAAAGCCAATGTGTGAAGTGAAAACCGACTTCT GTCAGATGTTGCTGCCATACTTGATCCATGATGTTTTACTGCAAGATACACATGAATCGTGGAGAACTCT GCTGTCTGCGCACGTCCGAGGATTTTTCACTAGTTGTTTTAAGCATTCCTCCCAAGCAAGCCGCTCAGCA ACTCCTGCAAATTCGGATTCAGAGTCAGAGAACTTTCTCCGATGCTGTTTGGATAAAAAGTCACAAAGAA CCATGCTTGCTGTTGTCGACTATCTGAGAAGGCAAAAGAGACCTTCCTCGGGAACAGCTTTTGATGACGC TTTCTGGCTGGATTTGAATTATCTTGAGGTTGCGAAGGTGGCTCAGTCCTGCTCTGCTCACTTCACGGCC TTGCTCTACGCAGAGATCTATTCAGATAAGAAAAGCACAGACGAGCAAGAGAAAAGAAGTCCAACATTTG AAGAAGGAAGTCAAGGAACAACTATTTCTAGTTTGAGTGAAAAAAGTAAAGAAGAAACTGGAATAAGCTT ACAGGATCTTCTCTTAGAGATCTACAGAAGTATAGGAGAGCCGGACAGCCTGTATGGCTGTGGAGGAGGG AAAATGTTACAACCCCTTACTAGAATACGGACATATGAACATGAAGCTACGTGGGAGAAAGCCTTAGTAA CTTACGACCTGGAGACCAGCATCTCCTCCTCCACCCGCCAGTCAGGAATCATCCAGGCCCTGCAGAATTT GGGGCTCTCCCATATCCTGTCTGTCTATCTGAAAGGATTAGACTATGAAAGACGAGAGTGGTGCGCTGAG CTGCAGGAGCTGCGTTACCAGGCGGCGTGGAGGAACATGCAGTGGGGCCTCTGCGCTTCTGCCGGCCAAG AAGTAGAAGGAACCAGTTACCATGAATCGTTGTATAATGCTCTGCAGTGTCTAAGAAACAGAGAATTCTC CACATTTTATGAAAGTCTCCGATATGCCAGTCTTTTCAGGGTGAAAGAAGTTGAAGAGTTGAGTAAGGGC AGCCTTGAGTCTGTATATTCGCTGTATCCCACACTTAGTAGATTGCAGGCAATTGGAGAACTGGAAAACA GTGGCGAGCTTTTCTCAAGGTCAGTCACAGACAGAGAGCGCTCTGAAGCATACTGGAAGTGGCAGAAGCA CTCCCAGCTTCTGAAAGACAGCGACTTCAGCTTTCAGGAGCCTCTCATGGCTCTGCGCACAGTCATTCTG GAGACCCTGGTACAGAAGGAAATGGAGCGCTCTCAAGGAGCATGCTCTAAGGACATTCTCACCAAACACC TCGTTGAATTCTCTGTTCTGGCTCGAACCTTCAAGAACACACAGCTCCCTGAAAGAGCAATATTCAAAAT TAAGCAATATAATTCAGCTATTTGTGGAATTTCTGAGTGGCATTTGGAAGAAGCACAAGTATTCTGGGCA AAAAAGGAGCAGAGTCTTGCTCTGAGTATTCTCAAGCAGATGATCAAGAAGTTGGACTCCAGCTTTAAAG ATAAAGAGAATGATGCAGGTCTCAAAGTCATATACGCAGAGTGTCTGAGGGTTTGTGGCAGCTGGCTGGC AGAAACTTGCTTAGAAAACCCTGCAGTCATCATGCAGACCTATCTAGAAAAGGCGGTGAAGGTTGCTGGA AGTTACGATGGCAACAGCAGAGAGCTCAGAAATGGACAGATGAAGGCCTTTCTCTCGTTGGCAAGGTTCT CTGATACTCAGTACCAGAGAATTGAAAACTACATGAAGTCATCAGAATTTGAAAACAAGCAAACTCTCTT AAAAAGAGCCAAAGAGGAAGTGGGCCTTCTAAGGGAACATAAAATTCAGACCAACAGATACACAGTAAAG GTTCAGCGAGAACTGGAGCTGGACGAATGTGCTCTCCGTGCACTGAGAGAGGATCGCAAGCGCTTCCTGT GTAAAGCAGTGGAGAACTACATCAACTGCTTACTAAGCGGGGAAGAACATGATCTGTGGGTGTTCCGGCT TTGCTCCCTCTGGCTTGAAAATTCTGGAGTTTCTGAAGTCAATGGCATGATGAAGAAAGATGGAATGAAG ATTTCATCCTATAAGTTTTTGCCTCTCATGTATCAATTGGCTGCTCGAATGGGGACCAAAATGACGGGAG GCCTAGGATTTCACGAAGTCCTCAATAATCTAATCTCTAGGATTTCACTGGATCACCCCCATCATACTTT GTTCATTATACTGGCCTTAGCAAATGCGAACAAAGATGAATTTTTGAGCAAACCAGAGACAACAAGAAGG AGTCGAATAACCAAAAGTACATCTAAAGAAAACTCTCACCTTGATGAGGATCGAACAGAGGCTGCAACCA GAATCATCCACTCCATCAGAAGTAAGCGATGTAAGATGGTGAAGGACATGGAGGCGCTCTGCGATGCCTA CATCATCTTGGCAAACATGGACGCCTCTCAGTGGAGGGCTCAGAGAAAAGGCATCAATATTCCAGCCAAC CAGCCAATCACTAAACTGAAGAATTTAGAAGATGTTGTTGTTCCCACTATGGAAATTAAGGTTGATCCCA CAGGAGAGTATGAAAATCTGGTGACTATAAAATCATTTAAAACAGAATTTCGCTTAGCTGGAGGCTTAAA TTTACCCAAAATAATAGATTGTGTGGGTTCTGATGGCAAGGAAAGGAGACAGCTTGTGAAGGGCCGTGAT GACCTGAGGCAAGATGCTGTCATGCAGCAGGTCTTCCAGATGTGCAATACACTACTGCAGAGAAACACTG AGACTAGAAAGAGGAAACTGACTATCTGCACATACAAGGTGGTTCCCCTTTCTCAGCGAAGCGGTGTTCT CGAGTGGTGCACAGGAACCGTTCCTATTGGTGAATATCTTGTTAACAGCGAAGACGGTGCACATAGAAGA TACAGGCCAAATGATTTCAGTGCCAATCAGTGCCAAAAGAAAATGATGGAAGTGCAGAAGAAGTCTTTTG AAGAGAAATATGATACCTTCATGACGATTTGCCAAAACTTTGAACCAGTTTTCCGTTACTTCTGCATGGA AAAATTCTTGGACCCAGCTGTTTGGTTTGAGAAACGATTGGCATATACACGCAGTGTGGCCACATCTTCT ATCGTCGGTTACATCCTTGGACTTGGCGACAGGCACGTACAGAATATCTTGATAAACGAGCAGTCGGCAG AGCTTGTGCACATAGACCTGGGAGTGGCTTTTGAACAGGGGAAGATCCTTCCCACTCCAGAAACAGTTCC TTTTAGACTCAGCAGAGATATTGTGGACGGGATGGGCATCACCGGTGTGGAAGGTGTCTTCAGAAGGTGC TGTGAAAAAACGATGGAAGTTATGCGGAGTTCTCAGGAAACCCTGCTGACCATTGTAGAGGTTCTTTTGT ACGATCCACTCTTTGATTGGACTATGAATCCTTTAAAAGCTCTGTATCTACAGCAGAGACCAGAAGATGA GTCCGACCTCCATTCCACCCCCAATGCAGATGATCAAGAATGCAAACAAAGTCTTAGTGATACTGACCAG AGTTTCAACAAAGTAGCTGAGCGTGTCTTGATGAGACTGCAAGAGAAACTGAAAGGCGTGGAGGAAGGCA CTGTGCTCAGTGTGGGTGGACAGGTGAACTTGCTTATCCAGCAGGCCATGGATCCCAAAAATCTCAGCCG ACTCTTCCCAGGATGGAAAGCTTGGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007499
Insert Size	9201 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007499.2, NP_031525.2
RefSeq Size	11964 bp
RefSeq ORF	9192 bp
Locus ID	11920
UniProt ID	Q62388
Gene Summary	Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Binds DNA ends. Plays a role in replication-dependent histone mRNA degradation. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.