Prune2 (NM_181348) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330414G02Rik; A230083H22Rik; A330102H22Rik; Bmcc1; mKIAA0367
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225458 representing NM_181348 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAATTTTTGCAACGCGCCAAATCTAAACTGGATCGAAGTAAACAATTGGAACAGGTCCACGCAG TTATTGGACCTAAGTCATGTGACCTGGATTCTCTCATTTCTGCCTTCACATATGCATACTTTCTGGACAA GGTCAGTCCTCCCGGGGTCCTCTGCTTGCCAGTGCTGAATATCCCAAGAACTGAATTCAACTACTTCACA GAGACAAGGTTTATTTTAGAAGAGCTGAACATCCCTGAATCATTCCACATATTCCGAGATGAGATTAACC TGCATCAGCTGAACGATGAAGGGAAACTGTCGATAACGCTCGTGGGCAGCCACGTCCTGGGGAGTGAGGA CAGAACTTTGGAATCGGCAGTTGTCAGAGTCATTAACCCCGGAGAGCAGAGTGATGGTGAGCTGGGGTTC CCGGAGACTTCCTCCTCTCTGGTGCTGAAGGAGCTCCTGCGTGAGGCTCCAGAACTCATCACCCAGCAGC TGGCACATCTCCTCAGAGGCAGCATCCTGTTCACGTGGATGAGTATGGATCCTGAGCTCCCGGAGAAGCA GGAGGAAATCCTCTCCATCCTGGAAGAACAGTTTCCCAACCTCCCTCCGAGGGATGATATCATCAACGTG CTGCAAGAGAGTCAGCTCAGTGCTCAGGGTTTAAGTCTTGAACAAACAATGCTGAAAGATCTAAAGGAGC TGTCAGACGGTGAAATAAAAGTGGCCATCAGCACCGTGAACATGACCCTGGAGGACTACCTGCTTCACGG CAACATCACCAGTGATTTAAAAGCCTTCACAGACAAGTTCGGCTTTGACGTCCTCATTCTGATCTCAAGC TTCACATGGGAGGAACAGCAGAGGCAGCAGATCGCCGTGTACTCCCAGAACCTGGAACTGTGCAGCCAGA TTTGCTGTGAGCTAGAAGAAAGTCAGAACCCTTGCCTGGAACTGGAGCCCTTCGAATGTGGCTGTGATGA GATCCTGGTATACCAGCAGGAAGACCCTTCTGTGACCTCCGACCAGGTGTTTCTTCTTCTTAAGGAAGTC ATCAATAGGAGGTGTGCAGAGATGGTCTCTAACAGTCGGACATCCTCAACAGAAGCCGTGGCAGGCAGCG CCCCGCTGTCCCAGGGCTCTTCCGGGATTATGGAATTGTATGGATCTGACATAGAGCCACAGCCCAGCTC TGTGAATTTCATAGAAAACCCCCCAGAGCTCAACGATTCTAACCAAGCTCAGGCGGATGGCAACATAGAC CTGGTTAGCCCTGACAGTGGGTTGGCCACCATCAGAAGCAGTCGCTCGTCCAAGGAAAGCTCCGTCTTCC TCAGTGACGACAGCCCCGTGGGAGACGGTGGTGCGCCTCACCATAGCCTTCTCCCAGGATTTGACTCCTA CAGCCCCATTCCTGAAGGGATAGTTGCGGAGGAGCATGCTCACTCCGGGGAACACAGTGAACACTTTGAC CTCTTCAACTTTGATTCAGCGCCCATAGCTTCAGAGCAGTCCCAGCCGTCTTCCCACTCTGCAGACTACT CGCCAGAAGACGACTTCCCCAACAGCGATTCATCAGAAGGGAACCTCTCTGCCGGGCCTAAGGGGCTTGG TGAGATGGGGATCAACATGTCCAATTACTCCTCTAGTTCTCTTTTGTCAGAGGCTGGTAAAGACAGCCTT GTGGAATTTGATGAAGAGTTTATCCAGCGACAAGAAAGCTCAGGGGATAACTCTGAAAGAAATTTAAGCC TGACATGTTTTGCGGGAGAGGAGCCTTCTTCCCCTGAAAGGCTGAAGAATCCTGGAAAGATGATCCCACC AACACCTATGAACAGCTTTGTGGAAATCTCACCATCAAATGAGGAGCCAACTCCACTCTATCCTGAAGAT ATAATCCAAAATGCAATTGACACCGGACATCTGGGCCCACCTCAGGCCCGTGCACGGTGCAGAAGCTGGT GGGGTGGCTTGGAGATTGACTCTAAAAACGTTGTAGATACATGGAACGCCAGTGAACAAGAATCTGTCTT CCAGAGCCCTGAACCATGGAAAGATCCTAAGCCTGAGCCAGTTGAGAGGAGAACCTCAGATTCCACATTC CAACCAAAGAGTCTTGAATTCTCAAAGTCAGATCCTTGGGAATCTGAGTTTGGACAGCCTGAACTGGGCA ACAAAGAAGCCCAGGACCAGAAGGAGGAAAGCTTGCAATACCAGCACTTGCCTACAGTAAGGCCACATTT GACAGATGCCTCTCCTCATGGGACAAACCACTTGATAGAGGACTTTGCTGCTTTGTGGCATTCAGGACAC TCACCAACAACAATGCCTGAGCCCTGGGGAAACCCCACCGATGCTGGTGAAGCTGCAGTTACGATGTCCT TCCCCACCTGGGGTGCATTTGATAAAGAAGAAGATAATGCTGACACATTAAAGAATACTTGGAATCTCCA TCCCACTAACAACGAGACACCTTCAGGGCAGGAACCAAGTGAATGGGCTATGGGCCAAAGTGGGTTTTCA TTCCCTGCAGCAGACCTACTTGACAATCCCCTCATTGAGGTAAATAAAGATGCAGCTCCAGAAATCTGGG GCAAGAACAACAGCTCCAAGGATACCAGTCTTACGTCTGGAAGTCCCACTTCAGATCTGGGTCAAACGTG GAATAATTCTAAGCTACCAGGGGAAGACCAGAATGGCTTGGTGGATCCTAAAGCTACAGGGAAGGTATAT GAGAAGGAAGGGTCCTGGAGCCTCTTTGAGGAGAGTGCTAAGAAAAGAGGGGCTGATGTTTTAGCTCCTT GGGAAGATTCCTTCTTGTCCTATAAATGTTCTGATTACAGTGCATCCAACATAGGAGAAGATTCAGTCCC ATCTCCATTAGACACCAACTACTCCACCTCTGACTCTTACACATCACCAACATATGCTGGGGATGAAAAA GAAATAGCAAACAAGCCAGTTGACAAAGATAATGGTTTTGAGGCAAAAGATGCTGAATTTCCTGCAGAGG GGCTTGAGGTTCTTGCAACATCATCACAGCAGTCTCAGAGAAATCGAATTGGTTCTGGTCCTGGGAATCT AGACATGTGGGCATTGCCTCACACAGAAGATAAGCCTGAAGGAAATGACGCACATCACCCAGATAGCGAT GCGCTCAAGACGGAGCACGCAGAAGACAAAAATGCTTCCATGGAGGATGACGTGAGGGAAAGCAGCCCGT CCAGTTACGATGACCCTAGCATGATGCGACTGTACGAGGCAAACCGGCAGCTCACCCTTCTACACAGCAA CACCAACTCACGCCAGGCAGCCCCTGACAGCCTTGACACATGGAACCGAGTGACTCTGGAGGACACGCAG TCCACTGCAACGATCTCGGACATGGATAATGACTTGGATTGGGATGACTGCAGTGGGGGCGTGGCGATCA GTGGGGATGGTCAAGCAGAAGGTTACATAGCTGCAAACAGTGAACCTGAAACCCGGTTCTCAGTGAAGCA GCTGGAACCATGGGGTACAGAACATCAGGAAGCAAATCAGGTAGACTGGGATCTCTCTGCCTCTGCTGAG CCCACAGGGGATCCTGGTCCCAGTGAATACCAGACCCTAAATGAGAAAACTGGGCAGTTAATTGCAAACA GTATTTGGGATTCTGTCATGGGAGATAAAAATATGCCATCATTCAGATTACCTAGCCCACCAAATACTGT AGATATGGAACACGGCACTTGGCCTTCTGAAAGTCCCAGGCACTCAAATGGTAAGGACAGCCACATGTTA GAAGCCTCTAGACTCAGTGAGTCGGGAGGGCTTACGTCTCAACCTGTCAACCAGGACACGTGGGGAGACT CCCAAGGTGACACAGCATCCTCGGTGACCGGACTGGCAAGTCCAGAACACTTTGCACAGTCAGATCCATG GACAGGCCATACTTATGGGCAGAGTGAAAGTGAGATTGAAGGCCTGGTGGCCTCTGATTGTGAGCATCTT GACAAGGAGGCAGCGCTAGGCTCTGGAGTGAATGGTGCCCCGTGGGCTTTTGGAAAAAAGCCCAGGGACC AGGAATTCTCTTCTTCAGATGCATTTGAACACCAAGACATCTCCAGTGCATCAGGCAAAATCAGCTCTCT TTCAGTCACCTCCAGTCCTCAGAGTGAGGAACCAGGGGAGGCCTTAGAAGTCGAGAAGGAGCCTTTCATT CTAGACTCCCTTGCTGTGCAAACAGAGACATTCACATGGGATTTGCAAAGCAAAGACACCCATGAAGAAT CTCTAGTGGATCATAGAAATTTAGGTGAAGCCAACACAACATTGGATAGGATTAATCCAATGAAAAACAG GCCCTTATCTGGAATGGAACTTGAGAAAACTGAAGCCTGTACCATTCTGAAGCCAGAAAGAGCAAATGGA AAACTACTTTATGAATCTTCTCAAGACTTTGGTGTTTGGGATGGCCCGATGGATAGTGATGTGTGGGATA GTCATATAAGTTATGAGACAGCTATGAATTCTACAGGTCAGAGAACTGAGGAAAGATCTCTAGAAGCCCT TTCACCAGGAAACTATGACAGAGACAGCCTCTCTAGTGGGTGTACGCATTCCAGTGCATCAAGTCCTGAC CTACATGATTCCTCAGTAGCCTTGTCCTCCTGGACTTACGGACCCAGTGCTGAACATCAGAAAGAGAATC ATGATGATGCGAACAAACAGATTCACCAAGAATCAGAACTATTTACCACCGAAGCCCATGTAGATGTCAT TACTGAAATGAAGGACTTTGTAGAAAACAGGGAGGATGGCTTTGGAAAGATGTCCAATCAGGAAGATCCT CAATTCCCAGAGATTCCAAATGATCCCTTCAACAGTGGTCCCTCATCCTCTTCATCCAGCCTTGGAGCAG ATAAATATTCAGAATATTCTCATGCATATCAGGAAGGGGATCTAACGACTAAACGTCAAGAGGAAAATGA ACTTGGTTTTCTGGAAATTGTGGAGCCAGAGGGTACCAGGATCATATCCACAAGCTCAGGTTCTGGCAAT GACAGTGGGGGTGACGAAGAGTTGCTAGAGAAGGAGCTCCATTTGGCCACAGTTGCTCAGAGTGAAGCTG GGGCTTGCACTTCATTGCATGAACCTGCGTTCTCGGCCACAGAGCATTCCAAGCCTGAGTTTTCTGTCTT TGTGGGTAGTTTGGAGTCAATAGAGAAAGAGAACAAGTCAAGCCCGTTCAGTGACAGTCAACAGAGCAGC CCTGGTCAGTGGATTTTGTCACCACTCATGCAGGCTGACACACAGGACACATGTAAGGAGGAGACCAGAG CTGCAGAAACGGGGACAATGGATACCACGTGGCATGGATCTGCCAGCACGGAAGCTAAGAATGGGGACCC TGACAAATTGGAAATGCTTGGCTTCTCAGCTGACAGCACTGAGTGGTGGAACGCTGGGGCACAGGAAGGG AGAGCAAACGCCGGTATGTCTGCAGAGGAGTTGTCTAACTCAGAGGGTGAGCTAGAGCCTACTTCTCCAG TGTTCCAGAACGCTGGTCCGTGGAGCTTACCCATTCAGAATGATAGTGAACCTGTGGACACAGGCAGCAC CAATCCTTTCCGTGGTAAACTAAAGTCACCCGTCCTAGATAGTCATGGAGACAAGTCACAGGAGAAACTT TGGAACATTCAACCTAAGCAGCTGGATTCAGATGCCAATCAACTCAGCCAGCTTGTGATCCTGGACCAAA TGAAGGACAAAGATTCTGGACAGCAAACAGCCATGTCTCCTGCTGCTGGTGATCTTCCTGCAGAAACTCT CACCCAAGGGCAGGGCCGGGAATCTATGCTGTCTGTCTGGGACCGTGCAGAACCAGCATTAACTCACAGA GATGAAAATGGATGTGTTAGCACTGGGGTTTCACCCACTGAGTGCCAACAAGAGAACCAGTGGGAACCAG AAAAACCCTATCTCAGCCACGTGACACATTCAAGCACTCCCACAGAAAATGCCCTCGAGAGCAATGCCCC CACACAACTGATGAGGAAGCTGGACTCTGATTGGAATAGCCCCAGCCCCAGCGAACCCCAACATAACTTT GTTCCAGATATTTTACATGGCAACTTCGAAGAGGGTGGGCAGCTGGCCTCGGCGTCACCTGATTTGTGGA TGGATGTCAAGCAACCCTTCAGTTTTAAAGTAGATAGCGAGAATCCTGATATATTGACTCACTGTGACCA TGACAGCAACTCTCAGGCATCCAGCAGCCCTGACGTGTGCCACGATTCTGAAGGGGAGCAAAAGATGGAG AAGCATACTGCTGTTTACCTGGGACTTGAAGTGGAACCCAGTGAGTTTTCTCTCACCGAGCCAAACATGA ATGATGAACCAACTTGGGAACCTGAGCAGGAAAGTCTCCCACACAATTCAGAACTCCATTCCGAACACGC AATGCCCTTGCCTCCAATCGACAGTCAGAATGATATAAATAACTCATCTAAGCCTGCCTCTTCAAGAAGT TCCCCAGAACCCTCTGATATGCGCGGAGACAACAACACAAGTGTAACATCGATGGAAGAAGACACCAACC CAGAGGTAGAAGCAGTGGACAGCGTGACGATCCCAGGTCACTTCCCCAGAAGTGAAGACGCTGACACTTT TGAAGCTCACCAAGAGGTCAGTGTGGAAGTCGACGACTCTTGGGTGTCAAAAGACCTTTGTCCTGAAAGT CAGACAGGCACTAGAGCTCTGCTTGACTGTGAGCAACCGTTTGCTTCCGAGAGCCCTGCTGTACTTACGG ACATCTTCCTAACCTCAGACACCTGCCTGGATGTAAGCGAAGCTGCCTTGGATCACAGTTTCAGTGATGC CTCCGGCCTCAACACTTCCACAGGCACTATAGACGACATGAGTAAGCTGACTCTATCGGAAGGCCATCCC GAAACACCAGTTGACGGGGATGCAGGGAAGCAAGATGTCTGTTCATCTGAAGCTTCCTGGGGTGATTTTG AATATGATGCAATGGGTCAGAATATTGACGAAGAGCTGATGAGAGAGCCTGAACACTTCCTCTATGGTGG TGATCTTCCGTCAGAGGAAAGCGCGCTGAAGCAATCACTGACACCATACACACCCCCCTTTGATTTGTCC TATCTCACGGAACCTACTGGATGCACTGAAACTGCACAGGGAGCTGAGTCTCCAGGTGATGAATCTCTGG GAAGTGACGCGGCAGAGATGTTGCTTTCTGCTCTTCCAGATCACAGGGAGGAAGACAAGGCTGAGACCAA CATCAGGAAACCTAGATACCAGATGACTGTGTTGCATATTCATGAAGACCCTGAGGCTCTTTCATCACCT GTGGGAGGAACAGGTTCCAATAATGAGTCATCTCCATCAAACATTGACTGGGAAATAGAAACAGATAATT CAGATTCACCAGCAGGTGGCGACATGAAACCACCAAACGGCAAGGAGATACTGGAGTTAGAAGAAGATGA GAAAGTAATTCCCACGAAAGGGCCAAAGCAAACAGAATTAGAATACAAAGAAGAAAAACAGCCAGAGCAA AGTGAAGACCATCAGGTACTAGCTGTGGACTACATCCTTGTAAGCCATGAAAAAGATTCACCATTGAAAC CAGAGGCCAGGGAAGCAAGAGAAAACATACCCGAATTGGAACAATTATCCATAGGTTCCAGAGAAACAGG GCTACCAGAGACTCAGTTATCAGGCACCCCAGACACATGTCAGTCTGAGTTTTTAAATGATGTCAAAGTT CATTCTGCAGAGAGAATGTCTTCGTCTTCCAACCACGAGAGTGCATCTCTTGAAAACCCTGCACAAGACC AGAGTTGGATGGTCTTGAGCCATAGTGAGGTTGGTGACCCGCCTACAGAAACCAGGGACTCGGGGCCTGA GTCTCCTGGTAGAACTCCGGAGCCGTTCTTGAGTCTCAGTTTGGACAAGGGCCCCAAGTCCCAGGTTCTG GAAAGAAATAAGCCTCTAAATTCTTTAGCACTAGAGGAAGTTGCTGGTTTGAGCAGCCAATCAAGGAACA TCGAGAGACAAGGTCAAGCTGGTCTAGATGCAGTTCCAACCCAGGCGGCCACCCACGACAATGAATGGGA AATGCTTTCGCCACAGCTCTCCCGGAAAAACAGGAACCCTCCACAGGAAATGGAAGAAGAGACACAGTGC CCTGAGCCTGGACCCAGAAAGCCAAGACTGAAAGGACCCCCGTCAGAGGATGAAGGAATGGATATCCACT TCGAGGAGGGAGTGCTGAGTCCCAGTGCCGCAGACATGAGGCCCGAACCTCCCAATTCTCTGGATCTCAA TGGCAGTCACCCTAGAAGAATCAAGCTCACAGCCCCAAATATCAACCTCTCTCTGGACCAAAGTGAAGGA TCTATTCTCTCTGATGATAACCTAGACAGTCCGGATGAGATTGACATCAATGTGGATGAACTCGATACCC CTGATGAAGCAGACTCTTTTGAATATACTAACCATGAAGATCCCACGGCCAACAAAAGCTCTGGTCAAGA GTCAGAGTCTATTCCAGAATATACGGCTGAAGAGGAGCGAGAAGACAACCGGCTGTGGAGGACGGTGGTC ATCGGGGAGCAGGAGCAACGGATTGACATGAAGGTCATTGAACCCTACAGGAGAGTCATTTCTCACGGAG GATACTATGGGGACGGTCTGAATGCCATCATTGTGTTTGCTGCCTGCTTTCTGCCAGACAGCAGCCGGGC GGATTACCACTATGTCATGGAAAACCTTTTCCTATATGTAATAAGTACCTTAGAGCTGATGGTTGCTGAA GACTACATGATCGTGTACTTAAATGGTGCAACCCCAAGGCGGAAGATGCCAGGACTAGGTTGGATGAAGA AATGCTACCAGATGATTGACAGACGGTTGCGCAAGAATTTGAAGTCATTCATCATTGTTCACCCATCTTG GTTCATCAGGACAATCTTAGCAGTGACTCGGCCTTTTATAAGCTCTAAATTCAGCAGTAAAATTAAATAT GTGACTAGCTTATCTGAGCTCAGTGGCCTGATCCCCATGGACTGCATCCACATTCCAGAGAGCATCATCA AGTATGATGAAGAGAAATCTTTTAAGAGAAGTGTGAGACTGGATGAAGAACTGAGGGAGGCATCCGAAGC AGCTAAAACTAGCTGCCTTTACAATGATCCAGAAATGTCTTCTATGGAAAAGGACATTGACATGAAGCTG AAGGAGAAGCCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_181348
Insert Size	9255 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_181348.4, NP_851993.3
RefSeq Size	12512 bp
RefSeq ORF	9255 bp
Locus ID	353211
UniProt ID	Q52KR3
Gene Summary	May play an important role in regulating differentiation, survival and aggressiveness of the tumor cells.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.