Htt (NM_010414) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI256365; C430023I11Rik; Hd; Hdh; IT15
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225462 representing NM_010414 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACCCTGGAAAAGCTGATGAAGGCTTTCGAGTCGCTCAAGTCGTTTCAGCAGCAACAGCAGCAGC AGCCACCGCCGCAGGCGCCGCCGCCACCGCCGCCGCCGCCTCCGCCTCAACCCCCTCAGCCGCCGCCTCA GGGGCAGCCGCCGCCGCCACCACCGCCGCTGCCAGGTCCGGCAGAGGAACCGCTGCACCGACCAAAGAAG GAACTCTCAGCCACCAAGAAAGACCGTGTGAATCATTGTCTAACAATATGTGAAAACATTGTGGCACAGT CTCTCAGAAATTCTCCAGAATTTCAGAAACTCTTGGGCATCGCTATGGAACTGTTTCTGCTGTGCAGTGA CGATGCGGAGTCAGATGTCAGAATGGTGGCTGATGAGTGCCTCAACAAAGTCATCAAAGCTTTGATGGAT TCTAATCTTCCAAGGCTACAGTTAGAACTCTATAAGGAAATTAAAAAGAATGGTGCTCCTCGAAGTTTGC GTGCTGCCCTGTGGAGGTTTGCTGAGCTGGCTCACCTGGTTCGACCTCAGAAGTGCAGGCCTTACCTGGT GAATCTTCTTCCATGCCTGACCCGAACAAGCAAAAGACCGGAGGAATCAGTTCAGGAGACCTTGGCTGCA GCTGTTCCTAAAATTATGGCTTCTTTTGGCAATTTCGCAAATGACAATGAAATTAAGGTTCTGTTGAAAG CTTTCATAGCAAATCTGAAGTCAAGCTCTCCCACCGTGCGGCGGACAGCAGCCGGCTCAGCCGTGAGCAT CTGCCAACATTCTAGGAGGACACAGTACTTCTACAACTGGCTCCTTAATGTCCTCCTAGGTCTGCTGGTT CCCATGGAAGAAGAGCACTCCACTCTCCTGATCCTCGGTGTGTTGCTCACATTGAGGTGTCTAGTGCCCT TGCTCCAGCAGCAGGTCAAGGACACAAGTCTAAAAGGCAGCTTTGGGGTGACACGGAAAGAAATGGAAGT CTCTCCTTCTACAGAGCAGCTTGTCCAGGTTTATGAACTGACTTTGCATCATACTCAGCACCAAGACCAC AATGTGGTGACAGGGGCACTGGAGCTCCTGCAGCAGCTCTTCCGTACCCCTCCACCTGAACTCCTGCAAG CACTGACCACACCAGGAGGGCTTGGGCAGCTCACTCTGGTTCAAGAAGAGGCCCGGGGCCGAGGCCGCAG CGGGAGCATCGTGGAGCTTTTAGCTGGAGGGGGTTCCTCGTGCAGCCCTGTCCTCTCAAGAAAGCAGAAA GGCAAAGTGCTCTTAGGAGAGGAAGAAGCCTTGGAAGATGACTCGGAGTCCAGGTCAGATGTCAGCAGCT CAGCCTTTGCAGCCTCTGTGAAGAGTGAGATTGGTGGAGAGCTCGCTGCTTCTTCAGGTGTTTCCACTCC TGGTTCTGTTGGTCACGACATCATCACTGAGCAGCCTAGATCCCAGCACACACTTCAAGCAGACTCTGTG GATTTGTCCGGCTGTGACCTGACCAGTGCTGCTACTGATGGGGATGAGGAGGACATCTTGAGCCACAGCT CCAGCCAGTTCAGTGCTGTCCCATCCGACCCTGCCATGGACCTGAATGATGGGACCCAGGCCTCCTCACC CATCAGTGACAGTTCTCAGACCACCACTGAAGGACCTGATTCAGCTGTGACTCCTTCGGACAGTTCTGAA ATTGTGTTAGATGGTGCCGATAGCCAGTATTTAGGCATGCAGATAGGACAGCCACAGGAGGACGATGAGG AGGGAGCTGCAGGTGTTCTTTCTGGTGAAGTCTCAGATGTTTTCAGAAACTCTTCTCTGGCCCTTCAACA GGCACACTTGTTGGAAAGAATGGGCCATAGCAGGCAGCCTTCCGACAGCAGTATAGATAAGTATGTAACA AGAGATGAGGTTGCTGAAGCCAGTGATCCAGAAAGCAAGCCTTGCCGAATCAAAGGTGACATAGGACAGC CTAATGATGATGATTCTGCTCCTCTGGTACATTGTGTCCGTCTTTTATCTGCTTCCTTTTTGTTAACTGG TGAAAAGAAAGCACTGGTTCCAGACAGAGACGTGAGAGTCAGTGTGAAGGCCCTGGCCCTCAGCTGCATT GGTGCGGCTGTGGCCCTTCATCCAGAGTCGTTCTTCAGCAGACTGTACAAAGTACCTCTTAATACCACGG AAAGTACTGAGGAACAGTATGTTTCTGACATCTTGAACTACATCGATCATGGAGACCCACAGGTCCGAGG AGCTACTGCCATTCTCTGTGGGACCCTTGTCTACTCCATCCTCAGTAGGTCCCGTCTCCGTGTTGGTGAC TGGCTGGGCAACATCAGAACCCTGACAGGAAATACATTTTCTCTGGTGGACTGCATTCCTTTACTGCAGA AAACGTTGAAGGATGAATCTTCTGTTACTTGCAAGTTGGCTTGTACAGCTGTGAGGCACTGTGTCCTGAG TCTTTGCAGCAGCAGCTACAGTGACTTGGGATTACAACTGCTTATTGATATGCTGCCTCTGAAGAACAGC TCCTACTGGCTGGTGAGGACCGAACTGCTGGACACTCTGGCAGAGATTGACTTCAGGCTCGTGAGTTTTT TGGAGGCAAAAGCAGAAAGTTTACACCGAGGGGCTCATCATTATACAGGGTTTCTAAAACTACAAGAACG AGTACTCAATAATGTGGTCATTTATTTGCTTGGAGATGAAGACCCCAGGGTTCGACATGTTGCTGCAACA TCATTAACAAGGCTTGTCCCAAAGCTGTTTTACAAGTGTGACCAAGGACAAGCTGATCCAGTTGTGGCTG TAGCGAGGGATCAGAGCAGTGTCTACCTGAAGCTCCTCATGCATGAGACCCAGCCACCATCACACTTTTC TGTCAGCACCATCACCAGAATCTATAGAGGCTATAGCTTACTGCCAAGTATAACAGATGTCACCATGGAA AACAATCTCTCAAGAGTTGTTGCCGCAGTTTCTCATGAACTCATTACGTCAACAACACGGGCACTCACAT TTGGATGCTGTGAAGCCTTGTGTCTTCTCTCAGCAGCCTTTCCAGTTTGCACTTGGAGTTTAGGATGGCA CTGTGGAGTGCCCCCACTGAGTGCCTCTGATGAGTCCAGGAAGAGCTGCACTGTTGGGATGGCCTCCATG ATTCTCACCTTGCTTTCATCAGCTTGGTTCCCACTGGATCTCTCAGCCCATCAGGATGCCTTGATTTTGG CTGGAAACTTGCTAGCAGCGAGTGCCCCCAAGTCTCTGAGAAGTTCATGGACCTCTGAAGAAGAAGCCAA CTCAGCAGCCACCAGACAGGAGGAAATCTGGCCTGCTCTGGGGGATCGGACTCTAGTGCCCTTGGTGGAG CAGCTTTTCTCCCACCTGCTGAAGGTGATCAATATCTGTGCTCATGTCTTGGACGATGTGACTCCTGGAC CAGCAATCAAGGCAGCCTTGCCTTCTCTAACAAACCCCCCTTCTCTAAGTCCTATTCGACGGAAAGGGAA GGAGAAAGAACCTGGAGAACAAGCTTCTACTCCAATGAGTCCCAAGAAAGTTGGTGAGGCCAGTGCAGCC TCTCGACAATCAGACACCTCAGGACCTGTCACAGCAAGTAAATCATCCTCACTGGGGAGTTTCTACCATC TCCCCTCCTACCTCAAACTGCATGATGTCCTGAAAGCCACTCACGCCAACTATAAGGTCACCTTAGATCT TCAGAACAGCACTGAAAAGTTTGGGGGGTTCCTGCGCTCTGCCTTGGACGTCCTTTCTCAGATTCTAGAG CTGGCGACACTGCAGGACATTGGAAAGTGTGTTGAAGAGGTCCTTGGATACCTGAAATCCTGCTTTAGTC GAGAACCAATGATGGCAACTGTCTGTGTGCAGCAGCTATTGAAGACTCTCTTTGGGACAAACTTAGCCTC ACAGTTTGATGGCTTATCTTCCAACCCCAGCAAGTCTCAGTGCCGAGCTCAGCGCCTTGGCTCTTCAAGT GTGAGGCCCGGCTTATATCACTACTGCTTCATGGCACCATACACGCACTTCACACAGGCCTTGGCTGACG CAAGCCTGAGGAACATGGTGCAGGCGGAGCAGGAGCGTGATGCCTCGGGGTGGTTTGATGTACTCCAGAA AGTGTCTGCCCAATTGAAGACGAACCTAACAAGCGTCACAAAGAACCGTGCAGATAAGAATGCTATTCAT AATCACATTAGGTTATTTGAGCCTCTTGTTATAAAAGCATTGAAGCAGTACACCACGACAACATCTGTAC AATTGCAGAAGCAGGTTTTGGATTTGCTGGCACAGCTGGTTCAGCTACGGGTCAATTACTGTCTACTGGA TTCAGACCAGGTGTTCATCGGGTTTGTGCTGAAGCAGTTTGAGTACATTGAAGTGGGCCAGTTCAGGGAA TCAGAGGCAATTATTCCAAATATATTTTTCTTCCTGGTATTACTGTCTTATGAGCGCTACCATTCAAAAC AGATCATTGGAATTCCTAAAATCATCCAGCTGTGTGATGGCATCATGGCCAGTGGAAGGAAGGCCGTTAC ACATGCTATACCTGCTCTGCAGCCCATTGTCCATGACCTCTTTGTGTTACGAGGAACAAATAAAGCTGAT GCAGGGAAAGAGCTTGAGACACAGAAGGAGGTGGTGGTCTCCATGCTGTTACGACTCATCCAGTACCATC AGGTGCTGGAGATGTTCATCCTTGTCCTGCAGCAGTGCCACAAGGAGAATGAGGACAAGTGGAAACGGCT CTCTCGGCAGGTCGCAGACATCATCCTGCCCATGTTGGCCAAGCAGCAGATGCATATTGACTCTCATGAA GCCCTTGGAGTGTTAAATACCTTGTTTGAGATTTTGGCTCCTTCCTCCCTACGTCCTGTGGACATGCTTT TGCGGAGTATGTTCATCACTCCAAGCACAATGGCATCTGTAAGCACTGTGCAGCTGTGGATATCTGGAAT CCTCGCCATTCTGAGGGTTCTCATTTCCCAGTCAACCGAGGACATTGTTCTTTGTCGTATTCAGGAGCTC TCCTTCTCTCCACACTTGCTCTCCTGTCCAGTGATTAACAGGTTAAGGGGTGGAGGCGGTAATGTAACAC TAGGAGAATGCAGCGAAGGGAAACAAAAGAGTTTGCCAGAAGATACATTCTCAAGGTTTCTTTTACAGCT GGTTGGTATTCTTCTAGAAGACATCGTTACAAAACAGCTCAAAGTGGACATGAGTGAACAGCAGCATACG TTCTACTGCCAAGAGCTAGGCACACTGCTCATGTGTCTGATCCACATATTCAAATCTGGAATGTTCCGGA GAATCACAGCAGCTGCCACTAGACTCTTCACCAGTGATGGCTGTGAAGGCAGCTTCTATACTCTAGAGAG CCTGAATGCACGGGTCCGATCCATGGTGCCCACGCACCCAGCCCTGGTACTGCTCTGGTGTCAGATCCTA CTTCTCATCAACCACACTGACCACCGGTGGTGGGCAGAGGTGCAGCAGACACCCAAGAGACACAGTCTGT CCTGCACGAAGTCACTTAACCCCCAGAAGTCTGGCGAAGAGGAGGATTCTGGCTCGGCAGCTCAGCTGGG AATGTGCAATAGAGAAATAGTGCGAAGAGGGGCCCTTATTCTCTTCTGTGATTATGTCTGTCAGAATCTC CATGACTCAGAACACTTAACATGGCTCATTGTGAATCACATTCAAGATCTGATCAGCTTGTCTCATGAGC CTCCAGTACAAGACTTTATTAGTGCCATTCATCGTAATTCTGCAGCTAGTGGTCTTTTTATCCAGGCAAT TCAGTCTCGCTGTGAAAATCTTTCAACGCCAACCACTCTGAAGAAAACACTTCAGTGCTTGGAAGGCATC CATCTCAGCCAGTCTGGTGCTGTGCTCACACTATATGTGGACAGGCTCCTGGGCACCCCCTTCCGTGCGC TGGCTCGCATGGTCGACACCCTGGCCTGTCGCCGGGTAGAAATGCTTTTGGCTGCAAATTTACAGAGCAG CATGGCCCAGTTGCCAGAGGAGGAACTAAACAGAATCCAAGAACACCTCCAGAACAGTGGGCTTGCACAA AGACACCAAAGGCTCTATTCACTGCTGGACAGATTCCGACTCTCTACTGTGCAGGACTCACTTAGCCCCT TGCCCCCAGTCACTTCCCACCCACTGGATGGGGATGGGCACACATCTCTGGAAACAGTGAGTCCAGACAA AGACTGGTACCTCCAGCTTGTCAGATCCCAGTGTTGGACCAGATCAGATTCTGCACTGCTGGAAGGTGCA GAGCTGGTCAACCGTATCCCTGCTGAAGATATGAATGACTTCATGATGAGCTCGGAGTTCAACCTAAGCC TTTTGGCTCCCTGTTTAAGCCTTGGCATGAGCGAGATTGCTAATGGCCAAAAGAGTCCCCTCTTTGAAGC AGCCCGTGGGGTGATTCTGAACCGGGTGACCAGTGTTGTTCAGCAGCTTCCTGCTGTCCATCAAGTCTTC CAGCCCTTCCTGCCTATAGAGCCCACGGCCTACTGGAACAAGTTGAATGATCTGCTTGGTGATACCACAT CATACCAGTCTCTGACCATACTTGCCCGTGCCCTGGCACAGTACCTGGTGGTGCTCTCCAAAGTGCCTGC TCATTTGCACCTTCCTCCTGAGAAGGAGGGGGACACGGTGAAGTTTGTGGTAATGACAGTTGAGGCCCTG TCATGGCATTTGATCCATGAGCAGATCCCACTGAGTCTGGACCTCCAAGCCGGGCTAGACTGCTGCTGCC TGGCACTACAGGTGCCTGGCCTCTGGGGGGTGCTGTCCTCCCCAGAGTACGTGACTCATGCCTGCTCCCT CATCCATTGTGTGCGATTCATCCTGGAAGCCATTGCAGTACAACCTGGAGACCAGCTTCTCGGTCCTGAA AGCAGGTCACATACTCCAAGAGCTGTCAGAAAGGAGGAAGTAGACTCAGATATACAAAACCTCAGTCATG TCACTTCGGCCTGCGAGATGGTGGCAGACATGGTGGAATCCCTGCAGTCAGTGCTGGCCTTGGGCCACAA GAGGAACAGCACCCTGCCTTCATTTCTCACAGCTGTGCTGAAGAACATTGTTATCAGTCTGGCCCGACTC CCCCTAGTTAACAGCTATACTCGTGTGCCTCCTCTGGTATGGAAACTCGGGTGGTCACCCAAGCCTGGAG GGGATTTTGGCACAGTGTTTCCTGAGATCCCTGTAGAGTTCCTCCAGGAGAAGGAGATCCTCAAGGAGTT CATCTACCGCATCAACACCCTAGGGTGGACCAATCGTACCCAGTTCGAAGAAACTTGGGCCACCCTCCTT GGTGTCCTGGTGACTCAGCCCCTGGTGATGGAACAGGAAGAGAGCCCACCAGAGGAAGACACAGAAAGAA CCCAGATCCATGTCCTGGCTGTGCAGGCCATCACCTCTCTAGTGCTCAGTGCAATGACCGTGCCTGTGGC TGGCAATCCAGCTGTAAGCTGCTTGGAGCAACAGCCCCGGAACAAGCCACTGAAGGCTCTCGATACCAGA TTTGGAAGAAAGCTGAGCATGATCAGAGGGATTGTAGAACAAGAAATCCAAGAGATGGTTTCCCAGAGAG AGAATACTGCCACTCACCATTCTCACCAGGCGTGGGATCCTGTCCCTTCTCTGTTACCAGCTACTACAGG TGCTCTTATCAGCCATGACAAGCTGCTGCTGCAGATCAACCCAGAGCGGGAGCCAGGCAACATGAGCTAC AAGCTGGGCCAGGTGTCCATACACTCCGTGTGGCTGGGAAATAACATCACACCCCTGAGAGAGGAGGAAT GGGATGAGGAAGAAGAGGAAGAAAGTGATGTCCCTGCACCAACGTCACCACCTGTGTCTCCAGTCAATTC CAGAAAACACCGTGCCGGGGTTGATATTCACTCCTGTTCGCAGTTTCTGCTTGAATTGTACAGCCGATGG ATCCTGCCATCCAGTGCAGCCAGAAGGACCCCCGTCATCCTGATCAGTGAAGTGGTTCGATCTCTTCTTG TAGTGTCAGACTTATTCACCGAACGTACCCAGTTTGAAATGATGTATCTGACGCTGACAGAACTACGGAG AGTGCACCCTTCAGAAGATGAGATCCTCATTCAGTACCTGGTGCCTGCCACCTGTAAGGCAGCTGCTGTC CTTGGAATGGACAAAACTGTGGCAGAGCCAGTCAGCCGCCTACTGGAGAGCACACTGAGGAGCAGCCACC TGCCCAGCCAGATCGGAGCCCTGCACGGCATCCTCTATGTGTTGGAGTGTGACCTCTTGGATGACACTGC AAAGCAGCTCATTCCAGTTGTTAGTGACTATCTGCTGTCCAACCTCAAAGGAATAGCCCACTGCGTGAAC ATTCACAGCCAGCAGCATGTGCTGGTAATGTGTGCCACTGCTTTCTACCTGATGGAAAACTACCCTCTGG ATGTGGGACCAGAATTTTCAGCATCTGTGATACAGATGTGTGGAGTAATGCTGTCTGGAAGTGAGGAGTC CACCCCCTCCATCATTTACCACTGTGCCCTCCGGGGTCTGGAGCGGCTCCTGCTGTCTGAGCAGCTATCT CGGCTAGACACAGAGTCCTTGGTCAAGCTAAGTGTGGACAGAGTGAATGTACAAAGCCCACACAGGGCCA TGGCAGCCCTAGGCCTGATGCTCACCTGCATGTACACAGGAAAGGAAAAAGCCAGTCCAGGCAGAGCTTC TGACCCCAGCCCTGCTACACCTGACAGCGAGTCTGTGATTGTAGCTATGGAGCGAGTGTCTGTTCTCTTT GATAGGATCCGCAAGGGATTTCCCTGTGAAGCCAGGGTTGTGGCAAGGATCCTGCCTCAGTTCCTAGATG ACTTCTTTCCACCTCAAGATGTCATGAACAAAGTCATTGGAGAGTTCCTGTCCAATCAGCAGCCATACCC ACAGTTCATGGCCACTGTAGTTTACAAGGTTTTTCAGACTCTGCACAGTGCTGGGCAGTCATCCATGGTC CGGGACTGGGTCATGCTGTCCCTGTCCAACTTCACACAAAGAACTCCAGTTGCCATGGCCATGTGGAGCC TCTCCTGCTTCCTTGTTAGCGCATCTACCAGCCCATGGGTTTCTGCGATCCTTCCACATGTCATCAGCAG GATGGGCAAACTGGAACAGGTGGATGTGAACCTTTTCTGCCTGGTTGCCACAGACTTCTACAGACACCAG ATAGAGGAGGAATTCGACCGCAGGGCTTTCCAGTCTGTGTTTGAGGTGGTGGCTGCACCAGGAAGTCCAT ACCACAGGCTGCTTGCTTGTTTGCAAAATGTTCACAAGGTCACCACCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010414
Insert Size	9363 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010414.3, NP_034544.1
RefSeq Size	13237 bp
RefSeq ORF	9363 bp
Locus ID	15194

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