Rph3al (NM_001291159) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm1753; Noc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225879 representing NM_001291159 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACACCATCTTCAGCAGTGGAAATGATCAGTGGGTTTGCCCCAACGATCGGCAGCTTGCTCTGA GAGCCAAGCTGCAGACGGGCTGGTCTGTGCACACGTACCAGACGGAGAAGCAGAGGAGGAGCCAGTGCCT GAGCCCCGGAGAGCTGGAGATCATCCTTCAGGTCATCCAGAGAGCAGAGAGGCTAGACATCCTGGAGCAG CAGAGAATTGGGCGGCTGGTGGAGCGGCTGGAGACAATGCAGAGGAACGTGATGGGCAACGGTCTCTCCC AGTGTCTGCTCTGCGGGGAGGTGCTGGGATTCCTGGGCAGCTCCTCCGTATTCTGCAAAGACTGCAGGAA GCTTGGGATCCGCTGGCTGCAGCACTTCCTAGAAAGCCTGCTGATTCTGAACAGAGATCTCACGTGCTCC TTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291159
Insert Size	426 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291159.1, NP_001278088.1
RefSeq Size	1708 bp
RefSeq ORF	426 bp
Locus ID	380714
UniProt ID	Q768S4
Gene Summary	Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells. Regulates the exocytosis of dense-core vesicles in neuroendocrine cells through interaction with RAB27A. Acts as a potential RAB3B effector protein in epithelial cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks several exons, and uses an alternate 3'-terminal exon, compared to variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.

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