Vamp7 (NM_001302138) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Sybl1; TI-VAMP; VAMP-7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226116 representing NM_001302138 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGGTGTGGAGGAAACTTCCTGGAGTTATTTGTTTCATTACATCTGCCAAGACAGGATTGTGTATC TTTGTATCACTGATGATGATTTTGAGCGTTCTCGAGCGTTCAGTTTTTTGAATGAAGTAAAGAAGAGATT CCAGACAACTTACGGTTCAAGAGCACAAACTGCACTTCCTTATGCTATGAATAGCGAGTTTTCAAGTGTT TTGGCTGCACAACTGAAGCATCACTCTGAGAATAAGAGCCTAGACAAAGTGATGGAGACTCAAGCACAAG TGGATGAACTGAAAGGAATAATGGTCAGAAACATAGATTTAGTTGCTCAACGTGGAGAAAGGTTGGAATT GCTGATAGATAAAACAGAAAACCTTGTGGATTCATCTGTCACTTTCAAAACTACCAGCAGAAATCTTGCT CGAGCCATGTGTATGAAGAATATCAAGCTCACTATCATCATCATCATTGTATCAATTGTGTTCATCTATA TCATTGTGTCACTTCTCTGTGGTGGGTTTACATGGCCAAACTGTGTGAAGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302138
Insert Size	546 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302138.1, NP_001289067.1
RefSeq Size	2530 bp
RefSeq ORF	546 bp
Locus ID	20955
UniProt ID	P70280
Gene Summary	Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.

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