Vps25 (NM_001284411) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110020N13Rik; AW107467; D11Wsu68e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226135 representing NM_001284411 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTACCGCTTCCCGCCCTTCTTTACGTTACAGCCGAACGTGG ACACCCGGCAGAAGCAGCTGGCCGCCTGGTGCTCTCTGGTTCTGTCCTTCTGCCGCCTGCACAAACAGTC CAGCATGACGGTGATGGAAGCCCAGGAGAGCCCGCTTTTCAACAACGTCAAGCTACAGCGCCATTCCCTT AACCTTAAGCCAGGGAAACTTCCTGTGGAGTCAATTCAGATTGTATTAGAAGAACTGAGAAAGAAAGGGA ACCTCGAGTGGTTGGATAAGAATAAGTCTAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAA ACTCATTTACCAATGGGTTTCCAGGAGTGGCCAGAATAACTCTGTGTTTACTCTGTATGAACTGACGAGT GGGGAAGACACAGAGGATGAAGAGTTCCATGGGCTGGACGAGGCGACCCTGCTGCGCGCTCTGCAGGCCC TACAGCAAGAGCACAAGGCTGAGATTATCACTGTCAGCGATGGCCGAGGAGTCAAGTTCTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284411
Insert Size	555 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284411.1, NP_001271340.1
RefSeq Size	1143 bp
RefSeq ORF	555 bp
Locus ID	28084
UniProt ID	Q9CQ80
Gene Summary	Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1).

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