Ing1 (NM_001302457) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610028J21Rik; AA407184; AI875420; mING1h; p33Ing1; p37Ing1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226136 representing NM_001302457 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGAGCTGGTGGAGAACCGCAGCAGACAGGTGGACAGTCACGTGGAGCTCTTCGAAGCACACCAGG ACATCAGTGACGGCACTGGTGGCAGCGGCAAGGCGGGCCAGGACAAGTCGAAGAGTGAGGCCATCACACA GGCAGATAAGCCGAATAACAAGCGGTCCAGGAGGCAGCGAAACAATGAGAATCGAGAGAACGCGTCGAAT AATCACGACCATGATGACATCACCTCAGGAACGCCCAAGGAGAAGAAAGCAAAAACCTCAAAGAAGAAGA AACGCTCCAAGGCCAAAGCAGAGAGGGAAGCGTCTCCTGCCGACCTTCCCATCGACCCCAACGAGCCCAC GTACTGTCTGTGCAACCAGGTCTCCTACGGGGAGATGATCGGCTGTGACAACGACGAATGCCCCATCGAG TGGTTCCACTTCTCCTGCGTGGGGCTCAACCATAAACCAAAGGGCAAGTGGTACTGCCCCAAGTGCCGTG GGGAGAGCGAGAAGACCATGGACAAAGCCCTGGAGAAGTCCAAGAAAGAGAGGGCTTACAACAGGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001302457
Insert Size	558 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302457.1, NP_001289386.1
RefSeq Size	1877 bp
RefSeq ORF	558 bp
Locus ID	26356
UniProt ID	Q9QXV3
Gene Summary	Isoform 1 inhibits p53-dependent transcriptional activation and may function as an oncoprotein. Isoform 2 acts as a negative growth regulator by cooperating with p53 in transcriptional activation of p53-responsive genes and may act as a tumor suppressor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an upstream alternate 5' terminal exon and lacks an exon in the 5' region, which results in the use of a downstream start codon, compared to variant 1. It encodes isoform 3, which has a shorter N-terminus, compared to isoform 1. Variant 3, 4, 5 and 6 encode the same protein.

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