Cbx5 (NM_001076789) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610029O15Rik; C75991; HP1; Hp1a; Hp1alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226181 representing NM_001076789 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAAGAAGACCAAGAGGACAGCCGACAGCTCTTCTTCAGAGGATGAGGAGGAATATGTGGTGGAAA AGGTGTTGGACAGGCGCATGGTTAAGGGGCAAGTGGAATATCTGTTGAAGTGGAAAGGCTTTTCTGAGGA GCACAATACTTGGGAACCTGAGAAGAACTTGGATTGTCCTGAACTAATTTCTGAGTTTATGAAAAAGTAT AAGAAGATGAAGGAGGGTGAAAACAATAAGCCCAGGGAGAAATCAGAAGGAAACAAGAGGAAATCCAGTT TCTCCAACAGCGCTGATGATATTAAATCTAAAAAAAAGAGAGAGCAAAGCAATGATATCGCTCGGGGCTT TGAGAGAGGACTGGAACCAGAAAAGATCATCGGAGCAACAGATTCCTGCGGTGACTTAATGTTCTTAATG AAATGGAAAGACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAACGTGAAGTGTCCACAGATTG TGATAGCATTTTATGAAGAGAGACTGACGTGGCACGCATATCCAGAGGATGCGGAAAACAAAGAAAAAGA AAGCGCGAAGAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001076789
Insert Size	576 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001076789.1, NP_001070257.1
RefSeq Size	8801 bp
RefSeq ORF	576 bp
Locus ID	12419
UniProt ID	Q61686
Gene Summary	Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.