Hmgb3 (NM_001293625) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Hmg2a; Hmg4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226255 representing NM_001293625 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAAGGTGACCCCAAGAAACCAAAGGGCAAGATGTCTGCTTATGCCTTCTTTGTGCAGACATGCA GGGAAGAACATAAGAAGAAAAACCCAGAGGTTCCCGTCAATTTTGCTGAGTTCTCCAAGAAGTGCTCGGA GAGGTGGAAGACCATGTCTAGCAAAGAGAAATCAAAGTTTGATGAAATGGCAAAGGCAGATAAAGTCCGA TATGATCGGGAGATGAAAGATTATGGACCAGCTAAAGGAGGCAAGAAGAAGAAGGACCCAAATGCCCCCA AAAGACCTCCGTCTGGATTTTTCTTATTCTGCTCTGAATTCCGCCCCAAGATCAAATCCACAAACCCTGG CATCTCCATTGGAGATGTGGCAAAAAAGCTGGGTGAGATGTGGAATAACTTAAGTGACAATGAAAAGCAG CCTTATGTCACCAAGGCAGCAAAGCTGAAGGAGAAGTATGAGAAGGATGTTGCTGACTATAAGTCTAAAG GGAAGTTTGATGGTGCCAAGGGTCCTGCTAAAGTTGCCCGGAAAAAGGTGGAAGAAGAGGAAGAGGAGGA GGAAGAGGAAGAAGAGGAGGAGGAAGAGGAGGAAGATGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293625
Insert Size	603 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293625.1, NP_001280554.1
RefSeq Size	1565 bp
RefSeq ORF	603 bp
Locus ID	15354
UniProt ID	O54879
Gene Summary	Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (PubMed:19890330). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (PubMed:12714519, PubMed:15358624, PubMed:16945912).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein.

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