Pdyn (NM_001286502) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Dy; Dyn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226553 representing NM_001286502 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGGTCCAGGCTGATGCTGGCAGCTTGCCTCCTCGTGATGCCCTCTAATGTTATGGCGGACTGCC TGTCCCTGTGCTCCCTGTGTGCAGTGAGGATTCAGGATGGGCCCCGTCCCATCAACCCCCTGATTTGCTC CCTGGAGTGCCAGGACCTGGTGCCGCCCTCAGAGGAGTGGGAGACATGCCGGGGCTTCTCATCTTTTCTC ACCCTGACGGTCTCTGGGCTCCGTGGCAAGGATGACTTGGAAGATGAGGTTGCTTTGGAAGAAGGCTACA GTGCACTAGCCAAGCTCTTGGAACCCGTCCTGAAGGAGCTGGAGAAAAGCCGACTCCTTACCAGCGTCCC AGAGGAAAAGTTCAGGGGTCTCTCCAGCAGCTTTGGCAACGGAAAAGAATCTGAGCTGGCGGGTGCTGAC CGGATGAATGATGAAGCCGCACAGGCGGGCACGCTCCATTTTAATGAGGAGGACTTGAGAAAACAGGCCA AACGCTATGGCGGCTTTTTGCGCAAATACCCCAAGAGGAGTTCCGAGATGGCCCGGGATGAGGACGGGGG CCAGGATGGGGATCAGGTAGGGCATGAGGACCTGTACAAACGCTATGGGGGCTTCCTGCGGCGCATTCGC CCCAAGCTGAAGTGGGACAACCAGAAGCGCTATGGTGGTTTCCTGCGGCGTCAGTTCAAGGTGGTGACGC GGTCCCAGGAGAACCCCAATACCTATTCTGAAGATTTAGATGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286502
Insert Size	747 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286502.1, NP_001273431.1
RefSeq Size	2316 bp
RefSeq ORF	747 bp
Locus ID	18610
Gene Summary	This gene encodes a preproprotein that is proteolytically cleaved to yield a number of active opium-like peptides. These peptides are the endogenous ligands for the Kappa-opioid receptor and similar G-protein-coupled receptors and are thought to function as the body's natural way to control addiction. These peptides have been associated with depression, stress, anxiety, response to pain, and maintenance of homeostasis via circadian rhythms and control of appetite. Mutations in the related human gene have been linked to the neurodegenerative disease spinocerebellar ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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