Dao (NM_001286397) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI987963; DAAO; DAMOX; Dao-1; Dao1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226563 representing NM_001286397 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGTGGCCGTGATCGGAGCAGGAGTCATTGGGCTCTCCACAGCCCTCTGCATTCATGAGCGTTACC ACCCAACACAGCCACTGCACATGAAGATCTATGCAGATCGATTCACCCCGTTCACCACGAGCGATGTGGC CGCCGGCCTCTGGCAGCCTTATCTCTCTGACCCCAGCAACCCTCAGGAGGCGGAGTGGAGCCAGCAAACG TTTGATTACCTGCTGAGCTGCCTCCATTCTCCAAACGCTGAAAAAATGGGCCTGGCCCTAATCTCAGGCT ACAACCTCTTCCGAGATGAAGTTCCGGACCCTTTCTGGAAAAACGCAGTTCTGGGATTCCGGAAGCTGAC CCCCAGTGAGATGGACCTGTTCCCTGATTATGGCTACGGCTGGTTCAATACAAGCCTCCTTCTAGAGGGG AAGAGCTACCTGCCATGGCTAACTGAGAGGTTAACTGAGAGGGGAGTGAAGCTTATCCATCGGAAGGTGG AGTCTCTCGAAGAGGTGGCAAGAGGAGTGGATGTGATTATCAACTGCACCGGGGTGTGGGCCGGGGCCCT GCAAGCAGATGCCTCCCTGCAGCCAGGCCGGGGCCAGATCATCCAGGTGGAGGCCCCTTGGATTAAACAC TTCATCCTCACCCATGATCCTAGCCTTGGTATCTACAACTCTCCGTACATCATCCCAGGAATGCAAGAAT TGTGGGTGAACTCACTGGCTTCCGGCCAGTCCGGCCTCAGGTCCGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286397
Insert Size	750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286397.1, NP_001273326.1
RefSeq Size	1751 bp
RefSeq ORF	750 bp
Locus ID	13142
Gene Summary	Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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