Zwint (NM_001293683) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2010007E07Rik; 2600001N01Rik; D10Ertd749e; Zwint-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226588 representing NM_001293683 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGCGGAGAAAAACGCTGTTGCGGAGAAAAACAACGCTGTTGCGACGAAAGAGGTTCTGGCTG AGGCAGCAGCCATTCTAGAACCTGTAGGCTTGCAGGAAGAAGCAGAGCTGCCTGCCAAGATCATGGAGGA GTTTATGAGGAACTCGCGGAAGAAAGACAAACTGCTCTGTAGCCAGCTTCAAGTAGTGAACTTCTTACAG ACTTTTCTGGCTCAGGAGGACACTGAGCAGAGCCCTGATGCTCTGGCTTCTGAAGATGCCAGTCGGCAAA AGGCAACTGAAACTAAGGAGCAGTGGAAGGACATGAAGGCCACATACATGGATCACGTGGATGTCATAAA ATGTGCCCTGTCTGAGGCCTTACCCCAGGTTAAAGAGGCTCACAGGAAGTACACAGAGCTTCAGAAGGCT TTTGAGCAACTCGAGGCTAAGAAGCGAGTCCTTGAGGAGAAACTTCAGCTGGCTCAGAAGCAGTGGGTAC TGCAACAGAAGCGTTTGCAGAACCTGACAAAGATTTCTGCAGAGGTCAAGAGGCGCCGGAAGAGGGCTCT GGAGAAGCTTGACGGATCCCATCAGGAACTGGAAACCTTGAAGCAGCAAGCGGGGCAGGAACAGGAGAAG CTGCAGAGGAACCAGAGCTACCTCCAGCTGCTGTGTTCGCTGCAGAATAAGCTGGTCATCTCTGAGGGCA AGGCTGAGGACAAAGATGTGAAAGGGCGAGCCCTTACAGCCAAATCCAAATCTCCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293683
Insert Size	759 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293683.1, NP_001280612.1
RefSeq Size	1851 bp
RefSeq ORF	759 bp
Locus ID	52696
UniProt ID	Q9CQU5
Gene Summary	Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.

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