Folr1 (NM_001252553) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	FBP1; Folbp-1; Folbp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226610 representing NM_001252553 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCACCTGATGACTGTGCAGTTGTTGCTCCTGGTGATGTGGATGGCCGAATGTGCTCAGTCCAGAG CTACTCGGGCCAGGACTGAACTTCTCAATGTCTGCATGGATGCCAAACACCACAAAGAAAAACCGGGCCC TGAGGACAATTTACACGACCAGTGCAGCCCCTGGAAGACGAATTCCTGCTGTTCCACGAACACAAGCCAG GAAGCACATAAGGACATTTCCTACCTGTACCGGTTCAACTGGAACCACTGCGGAACTATGACATCGGAAT GCAAACGGCACTTTATCCAAGACACCTGCCTCTATGAGTGTTCCCCGAACTTGGGACCCTGGATCCAGCA GGTGGACCAGAGCTGGCGCAAAGAGCGGATCCTTGATGTTCCCCTGTGCAAAGAGGACTGTCAGCAGTGG TGGGAGGACTGCCAGAGCTCTTTTACCTGCAAGAGCAATTGGCACAAGGGATGGAACTGGTCCTCGGGGC ATAACGAGTGTCCTGTGGGAGCCTCCTGCCATCCCTTCACCTTCTACTTCCCCACATCTGCTGCTCTGTG TGAGGAAATCTGGAGTCACTCCTACAAGCTCAGCAACTACAGTCGAGGGAGCGGCCGCTGCATTCAGATG TGGTTCGACCCAGCCCAGGGCAACCCCAACGAGGAAGTGGCGAGGTTCTATGCCGAGGCCATGAGTGGAG CTGGGTTTCATGGGACCTGGCCACTCTTGTGCAGCCTGTCCTTAGTGCTGCTCTGGGTGATCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252553
Insert Size	768 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252553.1, NP_001239482.1
RefSeq Size	1337 bp
RefSeq ORF	768 bp
Locus ID	14275
UniProt ID	P35846
Gene Summary	Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. Required for renal folate reabsorption.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses two different splice sites in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 all encode the same protein.

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