Pafah2 (NM_001285875) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310074E22Rik; AI507170
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226706 representing NM_001285875 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGCCTCTCGTGGCTTTGTGGTTGCTGCCGTAGAGCACAGGGACCAGTCAGCTGCGGCCACCT ACTTCTGCAAGCCGACCTCCCAGGAGAGCTCTCCCGCTGAATCTCTGGAGGAGGAGTGGCTCCCCTTCCG TCGCATCAAGGAAGGGGAGAAGGAGTTCCACGTCAGGAATCCTCAGGTGCACCAGCGGGTGAAAGAGTGT GTGCGAGTGCTGCGGATCCTTCAGGACGCCTCTGCTGGGAAGACTGTCGTCAATGTCTTCCCAGGAGGAC TGGATCTGATGACCTTGAAGGGCAGCATCGACAGGAACCGCGTAGCCGTGATGGGTCACTCCTTTGGAGG AGCTACGGCTGTTCTTGCTTTGACCCAGGAGGTCCAGTTTCGGTGTGCTATAGCCCTGGACGCTTGGATG TTCCCTCTGGAACGGGACTTTTACCCCAAAGCCCGGGGCCCTGTGTTCTTTATCAACGTCGAGAAATTCC AGACGGTAGAGAGCGTCAACTTGATGAAGAAGATCTGTGCCCAGCACGAGCAGTCCAGGATCGTGACTGT CCTAGGCGCTGTTCATCGGAGTCAAACTGACTTTGCCTTTGTGACGGGCAACCTGATTGCTAAATTCTTT TCCAGTAACTCTCGTGGTACCTTGGACCCTTACGAAAGTCAGGAGGTCATGGTGAGGGCCATCTTGGCCT TCCTGCAGAAGCACCTTGACCTGAAGGAAGACTACGATCAATGGAGCAGCTTCATCGAAGGCGTCGGACC ATCGCTCATCCAAGGGGCCCCGCACTATCTGTCTAGTCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285875
Insert Size	813 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285875.1, NP_001272804.1
RefSeq Size	3086 bp
RefSeq ORF	813 bp
Locus ID	100163
Gene Summary	Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an exon in the 5' region and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Both variants 4 and 5 encode isoform c.

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