Fhl2 (NM_001289533) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	FHL-2; SL; SLIM-3; SLIM3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226747 representing NM_001289533 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAACGCTTTGACTGCCACCACTGCAATGAATCTCTGTACGGCAAGAAGTACATTCTAAAGGAGG AGAACCCACACTGTGTGGCCTGCTTTGAGGAACTCTATGCCAATACCTGTGAGGAGTGTGGAACACCCAT CGGCTGCGACTGCAAGGACTTGTCCTACAAGGATCGGCACTGGCATGAAGGCTGCTTCCATTGCTCCCGG TGTGGAAGCTCGCTGGTGGACAAGCCCTTTGCTGCCAAGGAGGAACAGCTGCTGTGCACCGACTGCTATT CCAATGAATACTCGTCCAAGTGCCAGGAATGCAAGAAAACCATCATGCCAGGTACCCGCAAGATGGAATA CAAGGGCAGCAGCTGGCACGAGACCTGCTTCACCTGTCAGCGCTGCCAGCAGCCCATTGGAACCAAGAGC TTCATACCTAAGGAGAATCAGAACTTCTGCGTGCCCTGCTATGAGAAGCAGTATGCCCTGCAGTGCGTGC AGTGCAAAAAGCCTATCACCACAGGAGGTGTTACTTACCGGGAGCAGCCCTGGCACAAGGAATGCTTTGT GTGCACAGCCTGCAAGAAGCAGCTATCTGGGCAACGCTTCACAGCACGGGATGAGTTTCCATACTGCCTG ACCTGCTTCTGTGACTTGTACGCTAAGAAATGTGCTGGGTGCACCAACCCCATTAGTGGTCTGGGTGGCA CAAAGTACATCTCTTTCGAGGAACGCCAATGGCACAACGACTGCTTTAACTGTAAGAAGTGCTCCCTGTC TCTGGTGGGACGAGGCTTCCTCACAGAGAGAGATGACATCCTCTGTCCTGACTGTGGAAAGGATATTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289533
Insert Size	840 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289533.1, NP_001276462.1
RefSeq Size	1417 bp
RefSeq ORF	840 bp
Locus ID	14200
UniProt ID	O70433
Gene Summary	This gene encodes a member of the four-and-a-half-LIM-only protein family. The encoded protein functions as a regulator in numerous signaling pathways and cellular processes in development and cellular differentiation, including development and maintenance of the cardiovascular system and striated muscles. This gene also plays a role in bone formation and regulates and bone mineral content and bone mineral density. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.