Rnf144a (NM_001081977) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Rnf144; Ubce7ip4; UIP4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226842 representing NM_001081977 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACAGCAAGGTACCGGCCCACCTGGGACCTGGCCCTCGACCCACTGGTGTCCTGCAAGCTGTGCC TCGGGGAATACCCAGCGGAGCAGATGACAACCATCGCCCAATGCCAGTGCATCTTCTGTACCCTGTGCCT GAAACAGTATGTTGAGCTCTTGATCAAAGAAGGGCTAGAAACTGCAATTAGCTGCCCGGATGCTGCCTGT CCCAAACAGGGCCACCTTCAGGAGAATGAGATTGAGTGTATGGTTGCCGCAGAAATAATGCAGAGATATA AAAAGCTACAATTTGAAAGAGAGGTGCTCTTTGACCCTTGTCGGACGTGGTGCCCAGCATCCACCTGCCA GGCCGTGTGCCAACTCCAGGACATAGGGCTGCAGACCCCGCAGCTGGTACAGTGCAAAGCCTGTGACATG GAATTCTGCTCTGCTTGCAAAGCCCGCTGGCACCCTGGCCAAGGCTGCCCAGAGACCATGCCCATCACAT TCCTTCCCGGAGAGACCAGCTCTGCTTTCAAGATGGAAGAGGGCGACGCACCCATCAAACGCTGCCCCAA GTGCAGGGTGTACATCGAGCGGGATGAGGGCTGTGCACAGATGATGTGTAAGAACTGCAAGCATGCCTTC TGCTGGTACTGCCTGGAGTCTCTGGATGATGATTTCCTTCTGATACATTATGACAAGGGGCCCTGCCGCA ACAAGCTGGGCCACTCCCGAGCATCAGTGATCTGGCATCGGACACAGGTGGTGGGAATTTTTGCTGGATT TGGGCTCCTGCTCTTAGTGGCCTCCCCTTTCCTGCTCCTGGCCACACCCTTCGTGCTTTGCTGCAAATGC AAGTGCAGTAAAGGTGACGACGACCCACTGCCCACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081977
Insert Size	879 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081977.2, NP_001075446.1
RefSeq Size	5189 bp
RefSeq ORF	879 bp
Locus ID	108089
UniProt ID	Q925F3
Gene Summary	E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

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