Tssk4 (NM_001253889) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700020B19Rik; 4933424F08Rik; TSK-4; TSSK-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226845 representing NM_001253889 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGGAGACACCTCGGAAACAGCATCAGCCACCCCAGCCTATCGCTCTGTCATGGAGGAGTATG GTTATGAGGTGGGCAAGATCATTGGCCATGGCTCCTATGGAACTGTCTATGAGGCATACTACACAAAGCA GAAGGTCATGGTGGCTGTCAAGATCATCTCGAAGAAGAAGGCCTCTGAAGACTATCTCAACAAGTTCCTA CCACGTGAGATACAGGTAATGAAAGTCCTACGGCACAAGTACCTCATCAACTTCTATCAGGCCATTGAGA CCACATCCCGAGTATACATCATTTTGGAGCTGGCTCAGGGCGGTGATGTCCTCGAATGGATCCAACGATA TGGGGCCTGTGCTGAGACCCTTGCTGGCAAGTGGTTCTCCCAGATGGCTTTGGGCATCGCCTACCTGCAC AGCAAGGGCATCGTGCACCGCCTGACCCCCAGCCTTTCTGCTGCTGGTAGGGATTTAAAGTTGGAGAACC TGTTGCTGGACAAGCGGGAGAATGTGAAGATATCGGACTTTGGCTTCGCCAAGATGGTGCCTTCTAGCCA GCCTGTGCATAGTAGCCCTTCTTACCGCCAAATGAACAGCCTTTCCCACCTCAGCCAGACCTACTGTGGC AGCTTTGCTTACGCCTGCCCGGAGATCTTGCTAGGCTTGCCCTACAACCCTTTCCTGTCTGACACCTGGA GCATGGGCGTCATCCTCTACACTCTAGTGGTTGCACGGCTGCCCTTTGATGACACCAATCTCAAGAAGCT GCTGAGAGAAACCCAGAAGGAGGTCACTTTCCCAGCTAACTTGACCATCTCCCAGGAGTGCAAGGGCTCC AGCATAAAACCAGGGCCCCAGCCCCTTTCACCAGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253889
Insert Size	879 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253889.1, NP_001240818.1
RefSeq Size	1420 bp
RefSeq ORF	879 bp
Locus ID	71099
UniProt ID	Q9D411
Gene Summary	Isoform 1: Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (PubMed:17927909, PubMed:23599433, PubMed:23054012, PubMed:25361759, PubMed:26940607). May be involved in the Cre/Creb signaling pathway (PubMed:26940607). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells (By similarity). Phosphorylates CREM on 'Ser-116' in vitro (PubMed:26940607). Phosphorylates ODF2 on 'Ser-95' (PubMed:26961893).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses two alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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