Sparc (NM_001290817) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BM-40; ON
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226897 representing NM_001290817 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCCTGGCAGCCCCTCAGACTGAAGTTG CTGAGGAGATAGTGGAGGAGGAAACCGTGGTGGAGGAGACAGGGGTACCTGTGGGTGCCAACCCAGTCCA GGTGGAAATGGGAGAATTTGAGGACGGTGCAGAGGAAACGGTCGAGGAGGTGGTGGCTGACAACCCCTGC CAGAACCATCATTGCAAACATGGCAAGGTGTGTGAGCTGGACGAGAGCAACACCCCCATGTGTGTGTGCC AGGACCCCACCAGCTGCCCTGCTCCCATTGGCGAGTTTGAGAAGGTATGCAGCAATGACAACAAGACCTT CGACTCTTCCTGCCACTTCTTTGCCACCAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAGCTCCAC CTGGACTACATCGGACCATGCAAATACATCGCCCCCTGCCTGGATTCCGAGCTGACTGAATTCCCTCTGC GCATGCGTGACTGGCTCAAAAATGTCCTGGTCACCTTGTACGAGAGAGATGAGGGCAACAACCTCCTCAC TGAGAAGCAGAAGCTGCGTGTGAAGAAGATCCATGAGAATGAGAAGCGCCTGGAGGCTGGAGACCACCCC GTGGAGCTGTTGGCCCGAGACTTTGAGAAGAACTACAATATGTACATCTTCCCTGTCCACTGGCAGTTTG GCCAGCTGGATCAGCACCCTATTGATGGGTACCTGTCCCACACTGAGCTGGCCCCACTGCGCGCTCCCCT CATCCCCATGGAACATTGCACCACACGTTTCTTTGAGACCTGTGACCTAGACAACGACAAGTACATTGCC CTGGAGGAATGGGCCGGCTGCTTTGGCATCAAGGAGCAGGACATCAACAAGGATCTGGTGATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290817
Insert Size	906 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290817.1, NP_001277746.1
RefSeq Size	2638 bp
RefSeq ORF	906 bp
Locus ID	20692
Gene Summary	Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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