Fcer2a (NM_001253746) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CD23; Fce2; Fcer2; Ly-42
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226946 representing NM_001253746 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCTCAAAACCAGGGATACTGGGAACCTCCTAGAAAGCGTTGCTGCTGTGCAAGACGTGGGACAC AGCTCATGTTGGTGGGGCTGCTGAGCACAGCAATGTGGGCTGGCCTGCTGGCCCTGCTTCTTCTGTGGCA CTGGGAAACGGAGAAGAATCTAAAACAGCTGGGAGACACTGCAATTCAGAATGTCTCTCATGTTACCAAG GACTTACAAAAATTCCAGAGTAATCAATTGGCCCAGAAGTCCCAGGACTCTCGGCTCTCCCAGAACCTGA CCGGACTCCAGGAGGATCTAAGGAACGCCCAATCCCAGAACTCAAAACTCTCCCAGAACCTGAACAGACT CCAAGACGATCTAGTCAACATCAAATCCCTGGGCTTGAATGAGAAGCGCACAGCCTCCGATTCTCTAGAG AAACTCCAGGAAGAGGTGGCAAAGCTGTGGATAGAGATACTGATTTCAAAGGGAACTGCATGCAACATAT GTCCCAAGAACTGGCTCCATTTCCAACAGAAGTGCTACTATTTTGGCAAGGGCTCCAAGCAGTGGATCCA GGCCAGGTTCGCCTGCAGTGACCTGCAAGGGCGACTAGTCAGCATCCACAGCCAAAAGGAACAGGACTTC CTGATGCAACACATCAACAAGAAGGATTCCTGGATTGGCCTCCAGGATCTCAATATGGAGGGAGAGTTTG TATGGTCGGACGGGAGCCCTGTGGGTTATAGCAACTGGAATCCAGGGGAGCCCAATAACGGGGGCCAGGG TGAGGACTGTGTGATGATGCGGGGATCCGGCCAGTGGAACGACGCCTTCTGCCGCAGCTACTTGGATGCA TGGGTGTGTGAGCAGCTGGCAACATGTGAGATATCTGCCCCCTTAGCCTCTGTGACTCCAACAAGGCCCA CCCCAAAAAGTGAACCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253746
Insert Size	930 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253746.1, NP_001240675.1
RefSeq Size	2037 bp
RefSeq ORF	930 bp
Locus ID	14128
UniProt ID	P20693
Gene Summary	Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) has a different 5' end with an alternate start codon and lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform F) has a shorter and distinct N-terminus when it is compared to isoform A. Although variants 5 and 6 encode a protein of the same length (309 aa), the sequence of the two proteins (isoform E and isoform F) are different. Isoform F has also been called Fc epsilon receptor II subtype b variant b delta 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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