Mlst8 (NM_001252463) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610033N12Rik; AA409454; AI505104; AI851821; Gbl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227045 representing NM_001252463 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATACCACCCCAGGCACAGTGGGCAGTGACCCTGTCATCTTAGCAACTGCAGGCTATGACCACACGG TGCGCTTTTGGCAGGCTCACAGTGGAATCTGCACTCGAACAGTGCAGCATCAGGACTCTCAGGTAAATGC ATTGGAGATTACTCCGGACCGAAGCATGATTGCTGCTGCAGGTTACCAACATATCCGCATGTATGATCTC AACTCCAATAACCCCAACCCCATCATCAGTTATGACGGAGTCAGTAAGAACATTGCATCAGTGGGCTTTC ACGAGGATGGTCGCTGGATGTATACAGGTGGCGAGGACTGCACAGCTCGCATCTGGGACCTCAGGTCCCG GAACTTGCAGTGTCAGCGTATCTTCCAGGTGAACGCACCCATTAATTGCGTGTGTCTGCATCCCAACCAG GCAGAACTCATTGTGGGTGATCAGAGCGGTGCTATCCACATCTGGGACCTGAAGACAGACCACAATGAGC AGCTGATTCCCGAGCCTGAGTCTTCCATCACGTCTGCTCACATCGACCCAGATGCTAGCTACATGGCAGC CGTCAATAGTGCCGGAAACTGCTATGTCTGGAACCTGACAGGGGGCATTGGTGACGATGTGACTCAGCTC ATCCCTAAGACCAAGATCCCAGCCCATACACGCTATGCCCTGCAATGCCGCTTCAGCCCTGATTCCACGC TTCTTGCCACCTGTTCAGCTGACCAGACATGTAAAATCTGGAGGACATCCAACTTCTCCCTGATGACAGA GCTTAGCATCAAGAGTAGTAACCCTGGAGAGTCATCCCGTGGCTGGATGTGGGGCTGTGCCTTCTCAGGG GATTCCCAGTACATTGTCACAGCTTCTTCTGACAACCTAGCCCGGCTCTGGTGTGTAGAGACTGGAGAAA TCAAGAGAGAGTATGGTGGCCATCAGAAAGCTGTCGTCTGCTTGGCCTTCAATGACAGTGTGCTGGGTTA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252463
Insert Size	981 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252463.1, NP_001239392.1
RefSeq Size	3366 bp
RefSeq ORF	981 bp
Locus ID	56716
UniProt ID	Q9DCJ1
Gene Summary	Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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