Lef1 (NM_001276403) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3000002B05; AI451430; Lef-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227075 representing NM_001276403 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGGTGGTCAGACAAGCCCCGTCCTCTCAGGAGCCCTACCACGACAAGGCCAGAGAACACCCTG ATGAAGGAAAGCATCCAGACGGAGGCCTGTACAACAAGGGACCCTCCTACTCCAGTTACTCTGGCTACAT AATGATGCCCAATATGAACAGCGACCCGTACATGTCAAATGGGTCCCTTTCTCCACCCATCCCGAGGACA TCAAATAAAGTGCCCGTGGTGCAGCCCTCTCACGCGGTCCACCCGCTCACCCCCCTCATCACCTACAGCG ACGAGCACTTTTCTCCGGGATCCCACCCGTCACACATCCCGTCAGATGTCAACTCCAAGCAAGGCATGTC CAGACACCCTCCAGCTCCTGAAATCCCCACCTTCTACCCCCTGTCTCCGGGCGGCGTTGGACAGATCACC CCACCCATTGGCTGGCAAGGTCAGCCTGTTTATCCCATCACGGGTGGATTCAGGCAACCCTACCCATCCT CACTGTCAGGCGACACTTCCATGTCCAGGTTTTCCCATCATATGATTCCTGGTCCCCCTGGCCCCCACAC AACTGGCATCCCTCATCCAGCTATTGTAACACCTCAGGTCAAACAGGAGCACCCCCACACGGACAGTGAC CTAATGCACGTGAAGCCTCAACACGAACAGAGAAAGGAGCAGGAGCCCAAAAGACCTCATATTAAGAAGC CTCTGAATGCTTTCATGTTATATATGAAAGAAATGAGAGCGAATGTCGTAGCTGAGTGCACGCTAAAGGA GAGTGCAGCTATCAACCAGATCCTGGGCAGAAGATGGCACGCCCTCTCCCGGGAAGAGCAGGCCAAATAC TATGAACTAGCACGGAAAGAGAGACAGCTACACATGCAGCTTTATCCAGGCTGGTCAGCGCGAGACAATT ATGGCAAGAAGAAGAAGAGGAAGAGAGAGAAGCTACAGGAGTCGACTTCAGGTACAGGTCCCAGAATGAC AGCTGCCTACATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276403
Insert Size	996 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276403.1, NP_001263332.1
RefSeq Size	2305 bp
RefSeq ORF	996 bp
Locus ID	16842
Gene Summary	Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate 5' exon and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter and has a distinct N-terminus, compared to isoform a.

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