Mgme1 (NM_001289631) Mouse Untagged Clone

CAT#: MC227123

Mgme1 (untagged) - Mouse mitochondrial genome maintainance exonuclease 1 (Mgme1), transcript variant 3



  "NM_001289631" in other vectors (1)

CNY 3,330.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 8430406I07Rik; AI426476
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227123 representing NM_001289631
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACTGCCTCTGACCTTCTGCAGGCTGCTTAGCAGGTTAAACAGATTTTCTGTAAAAGCAAGCCCTC
CTGTGAGTTTCTCCACTTTCTCTTATTTGTGTAGCCAGAAGAAGAAAAACTCTTACGAAGCAGTAGACCA
AGCGAAGTACTCTCGTTTAGTACGCTCTGTCTTGTCCAGAGGCCCGGCCCAGACTCCAGAGTCGTTGTTC
AAGGAAGATGATGTACTGTATGGACCAGTGAGTAAGCATAAGGCTGCAGAGCCGGAGCCACAGGCCAGAG
TCCCACAACACTGCTTTCCTATCTTCAATGAAGAGAGAACCGGGAAACCACACACAGATGCTTCTTCAAG
CCCTTTGAAGATCCCTTTGCAAAGGAACTCGATACCCAGTGTGACCCGCATCCTTCAGCAGACCATGCCA
CCTGAACAGAGCTTCTTTTTGGAGAGGTGGAAAGAGCGGATGGTTCTGGAGCTGGGAGAAGACGGGTTTG
CAGAATATACTTCAAATGTGTTTTTACAAGGCAAACAGTTCCATAAAGCCTTGGAAAGCATACTGTCACC
CCAGGAGAACCTAACAGGGGGAGAAGAGCACCCCCAGTGTGGCTACATCGAAAGCATCCAGCATATTCTG
ACAGAAATCAGTGGTGTGCAAGCTCTGGAGAGTGCCGTCCAGCATGAGGCCTTGAAGTATGTAGGGCTGC
TGGACTGTGTGGCTGAGTACCGGGGCAAGCTGTGTGTGATTGATTGGAAGACATCAGAAAAACCAAAACC
TTTAATTCGAAATACATATGACAACCCGCTGCAAGTTGTGGCGTACATGGGTGCCGTAAACCATGATGCC
CACTACAGTTTTCAGGTTCAGTGTGGATTAATTGTGGTGGCCTATAAGGATGGGTCCCCTGCGCACCCTC
ACTTCATGGATGAAGAGCTCTGTTCCAAGTATTGGGCCAAGTGGCTTCTCCGACTAGAAGAATATACAGA
AAAGCAAAAGAACCTGAGCGCTCCAGAGCCAGCGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001289631
Insert Size 1017 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001289631.1, NP_001276560.1
RefSeq Size 2778 bp
RefSeq ORF 1017 bp
Locus ID 74528
UniProt ID Q9CXC3
Gene Summary Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All four variants encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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