Cysltr1 (NM_001281862) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BB147369; Cyslt1; CysLT1R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227128 representing NM_001281862 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAACTGAAAATCTGACGACATCTCTCATTAATAACACGTGTCATGACACAATTGATGAATTCC GAAATCAAGTATACTCCACTATGTATTCTGTGATCTCTGTTGTGGGTTTCTTTGGCAATAGCTTTGTGCT CTATGTCCTCATAAAAACATACCATGAGAAATCAGCCTTCCAAGTATACATGATTAATCTAGCCATAGCA GATCTACTCTGTGTATGTACATTGCCTCTCCGTGTGGTCTATTATGTTCACAAAGGCAAGTGGCTCTTTG GTGACTTTTTGTGCCGCCTCACCACCTATGCCTTGTACGTTAACCTCTATTGTAGCATCTTCTTTATGAC AGCCATGAGCTTTTTCCGGTGTGTTGCAATTGTCTTTCCAGTCCAGAACATTAATTTGGTTACACAGAAA AAAGCCAGGTTCGTTTGCATTGGAATTTGGATTTTTGTGATTTTGACAAGTTCTCCCTTTTTAATGTACA AATCTTACCAAGATGAGAAAAACAATACTAAATGCTTTGAGCCTCCACAGAACAATCAAGCTAAAAAATA CGTTTTGATCTTGCATTATGTGTCATTATTCTTTGGTTTCATCATCCCTTTTGTCACCATAATTGTCTGT TACACAATGATCATTCTGACCTTACTAAAAAATACAATGAAGAAAAACATGCCAAGTCGTAGGAAGGCTA TAGGGATGATCATAGTTGTGACAGCTGCCTTTTTAGTGAGCTTCATGCCATATCATATTCAACGAACTAT CCACCTTCACCTTTTACACAGTGAAACTAGACCCTGTGATTCTGTCCTTAGGATGCAGAAGTCAGTGGTC ATAACCTTATCTCTAGCTGCATCAAATTGTTGCTTTGATCCTCTGCTATATTTCTTTTCAGGTGGAAACT TTAGGAGAAGGCTATCTACATTTAGAAAGCATTCTTTGTCCAGTATGACTTATGTACCCAAGAAGAAAGC TTCCTTGCCAGAAAAAGGAGAAGAAATATGTAACGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281862
Insert Size	1020 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281862.1, NP_001268791.1
RefSeq Size	2802 bp
RefSeq ORF	1020 bp
Locus ID	58861
UniProt ID	Q99JA4
Gene Summary	Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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