Lpar1 (NM_001290486) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI326300; Edg2; Gpcr26; Kdt2; lpA1; vzg-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227182 representing NM_001290486 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGAACAACAGTGCTTCTACAATGAGTCTATCGCCTTCTTTTATAACCGGAGTGGGAAATATCTAG CCACAGAATGGAACACAGTGAGCAAGCTGGTGATGGGACTGGGCATCACTGTTTGCGTGTTCATCATGTT GGCCAATCTCCTGGTCATGGTGGCAATCTACGTCAACCGCCGCTTCCATTTCCCTATTTATTACTTGATG GCCAACCTGGCTGCTGCAGACTTCTTCGCTGGATTGGCCTACTTCTACCTGATGTTCAATACAGGACCTA ATACCCGGAGACTGACTGTTAGCACGTGGCTCCTCCGGCAGGGCCTCATTGACACCAGCCTGACAGCTTC TGTGGCCAACCTGCTGGCTATTGCTATCGAGAGGCACATCACGGTTTTCCGCATGCAGCTCCATACACGA ATGAGCAACCGGCGCGTGGTGGTGGTGATTGTAGTCATCTGGACTATGGCCATTGTGATGGGTGCTATAC CCAGTGTGGGCTGGAACTGCATCTGTGATATCGATCACTGTTCCAACATGGCACCCCTCTACAGTGACTC CTACTTAGTCTTCTGGGCCATTTTCAACCTGGTGACCTTTGTGGTCATGGTGGTTCTCTACGCTCACATC TTTGGCTATGTTCGCCAGAGGACTATGAGGATGTCTCGGCATAGTTCTGGACCCAGGAGGAATCGGGACA CCATGATGAGCCTTCTGAAGACTGTGGTCATTGTGCTTGGTGCCTTTATTGTCTGCTGGACTCCGGGATT GGTCTTGTTATTGCTGGATGTGTGCTGCCCGCAGTGCGATGTCCTGGCCTATGAGAAGTTCTTCCTCCTC CTGGCCGAGTTCAACTCTGCTATGAACCCCATCATCTACTCCTACCGCGACAAAGAGATGAGCGCCACCT TCAGGCAGATCCTGTGTTGCCAGCGCAACGAGAACCCTAATGGCCCCACGGAAGGCTCTGACCGCTCTGC CTCCTCCCTCAACCACACCATTCTGGCTGGAGTTCACAGCAACGACCACTCTGTGGTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290486
Insert Size	1041 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290486.1, NP_001277415.1
RefSeq Size	3247 bp
RefSeq ORF	1041 bp
Locus ID	14745
UniProt ID	P61793
Gene Summary	Receptor for lysophosphatidic acid (LPA) (PubMed:11087877, PubMed:18066075). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins (PubMed:8922387, PubMed:9600933, PubMed:11040035, PubMed:18157949, PubMed:18066075, PubMed:23478264). Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels (PubMed:11040035, PubMed:12215548). Signaling triggers an increase of cytoplasmic Ca(2+) levels (PubMed:12215548). Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate (PubMed:11040035, PubMed:12215548, PubMed:23478264). Signaling mediates activation of down-stream MAP kinases (PubMed:11040035). Contributes to the regulation of cell shape (PubMed:8922387, PubMed:9600933, PubMed:11040035, PubMed:11087877). Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction (PubMed:9600933, PubMed:11040035, PubMed:12181339). Promotes the activation of Rho and the formation of actin stress fibers (PubMed:9600933, PubMed:12215548). Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1 (PubMed:23478264). Through its function as lysophosphatidic acid receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding (PubMed:11087877, PubMed:18066075, PubMed:23478264). Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14 (PubMed:21821728). Promotes cell proliferation in response to lysophosphatidic acid (PubMed:9600933, PubMed:11087877, PubMed:12215548, PubMed:18157949, PubMed:17692995, PubMed:23478264). Required for normal skeleton development (PubMed:21569876). May play a role in osteoblast differentiation (PubMed:21569876). Required for normal brain development (PubMed:17656621, PubMed:18708146). Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus (PubMed:18708146). Plays a role in pain perception and in the initiation of neuropathic pain (PubMed:15195086, PubMed:19689455).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at a downstream start codon, compared to variant 2. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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