Gja5 (NM_001271628) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730555N10Rik; Cnx40; Cx40; Gja-5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227252 representing NM_001271628 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGACTGGAGCTTCCTGGGGGAGTTCCTGGAGGAGGTCCACAAGCACTCCACAGTCATCGGCAAGG TCTGGCTCACTGTCCTGTTCATTTTCCGCATGCTGGTCCTGGGCACCGCTGCTGAGTCCTCCTGGGGAGA TGAGCAGGCCGACTTCCGGTGCGATACCATTCAGCCTGGTTGCCAAAATGTCTGCTATGACCAAGCCTTC CCCATCTCCCACATTCGTTATTGGGTACTGCAGATCATCTTTGTGTCCACGCCTTCTCTAGTGTACATGG GCCATGCCATGCACACTGTGCGCATGCAGGAAAAGCAGAAATTGCGGGATGCTGAGAAAGCTAAAGAGGC CCACCGCACTGGTGCCTATGAGTACCCAGTAGCCGAAAAGGCCGAGCTGTCCTGCTGGAAAGAAGTAGAT GGGAAGATTGTCCTCCAGGGCACCCTACTCAACACCTATGTCTGCACCATTCTGATCCGCACCACCATGG AGGTGGCCTTCATCGTAGGCCAGTACCTCCTCTATGGGATCTTCCTGGATACCCTGCATGTCTGCCGCAG GAGTCCCTGTCCCCACCCAGTCAACTGTTATGTTTCGAGGCCCACGGAGAAGAATGTCTTCATTGTCTTT ATGATGGCTGTGGCTGGACTGTCTCTGTTTCTCAGCCTGGCTGAACTCTACCACCTGGGCTGGAAGAAGA TCCGACAGCGCTTTGGCAAGTCACGGCAGGGTGTGGACAAGCACCAGCTGCCTGGCCCTCCCACCAGCCT CGTCCAGAGCCTCACTCCTCCCCCTGACTTCAATCAGTGCCTAAAGAACAGCTCCGGAGAGAAATTCTTC AGCGACTTCAGTAATAACATGGGCTCCCGGAAGAATCCAGACGCTCTGGCCACTGGGGAAGTGCCAAACC AGGAGCAGATTCCAGGGGAAGGCTTCATCCACATGCACTATAGCCAGAAGCCAGAGTACGCCAGTGGAGC CTCTGCGGGCCACCGCCTTCCTCAGGGCTACCATAGTGACAAACGGCGCCTTAGTAAGGCCAGCAGCAAA GCAAGGTCAGATGACCTGTCAGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271628
Insert Size	1077 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271628.1, NP_001258557.1
RefSeq Size	3180 bp
RefSeq ORF	1077 bp
Locus ID	14613
UniProt ID	Q01231
Gene Summary	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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